LOCUS       sequence-406600-        9843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CBFB_MYH11 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CBFB_MYH11
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4604..5314,5315..5317,5318..5320))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4646..4667)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4995..5014
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5254..5275
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5317..5326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5402..5605)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5431..5451)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5606..5909
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6166..6210)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6395..6628
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6988..7105
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7597..7722
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7769..8026
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8028..8407
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8438..8542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8543..8611,8612..9403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8761..8780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9574..9843,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 cactagcctt cgttccgttg aaatctgcat cgcgtgtatc agtctcctcc tccgacccgt
     1861 ctgcgttttc aatgacgcga cggccccctg aacggcgact agggacgaaa cttgtctcgt
     1921 taccgcgacg caatttggat tttaacgcat taacctcacg gcccatagcc tcgttcgact
     1981 ccgtagcttc atcaagttca cgctgaagct tacggcggtt tgcattgata cgctgtgact
     2041 cctcttcagc ttcctccaat tgacgcttca attgtttaac gcgtgcgttc cctttctctg
     2101 cttgctcttt atattgctct gccatcttac gttcatcctc gacttgcagc agaatttcct
     2161 tcaacttttt atccttttgt ttaagagact tagttgcggc ctgcttttcg cgcgcttcct
     2221 gttcaacctg ttcttctaat tgtgcaattt tagcctccag cgctgcaatc gtacttttaa
     2281 acttgctttt caccgcgccc tccatttcgt gtaacttaga acgaagttct ttattctgac
     2341 gctccagttg ctgacgtgcg gattcattct tctgtgctgt gctacgttca gtagccaatt
     2401 cgttagacaa ctgctctgcc tgttgggtgg ctttacgcac acgatctgac atcgcttcca
     2461 tgtttccctg ctcctcctcc aattcttcct caagctgggc aatacgagcc tccaggcgac
     2521 gtttttcgtc ctgtaaggca ttacggccgg acagactcga agctaactcc tcggccagct
     2581 cctccttttc aaggtctgct tgcttacgcg cgcgttctgc ggctgccaaa tcttcctgca
     2641 gttgcatcaa gtcagcctcc aggctcttgg cttttttttc gttttcttta gctgtggcga
     2701 aaatttcgtc acgactagcg cgggcatctt ccagctcgcg ctggaagtcc ttcatttgcg
     2761 cctgcagttt gcgaagctgc ttgatagcct cttcgcggcc cttaattgcc gaatctgctt
     2821 gcaattctaa atccttcaga tcgccctcca gcttcttttt ggcggcagcc gccagcgcgc
     2881 gctgcttacg ttcatcctcc agctcagtct catactcatg cagttgacgc tgcagttggc
     2941 ggcgtttctc ctcgttctgt tcatcgcgcg cctgtaagtc acgctcaaac tgacctttta
     3001 aggcctgcat attgacctct aaacgaagct tagcatcctc ggtcgcctgc agctcgtctt
     3061 ccagctcttc aagctgagtt ttcatctctt ccatttgagt ttctaaggca cgctttgatt
     3121 tctctaactc atgcacattt ttacccacgt cgtcctttga actaacaagg tcctccattt
     3181 ccgctttcag cattttattg gtacgctcta actcctcctt ggcttccaag gcctcctcca
     3241 aagcacgtgc aaggctcaaa gccttagttt ccttttcgcg cgcctcagcc tcggcgcggt
     3301 cacgctcgtc agcgtacttt gatgaaatgt tcttctcttc tgctaacaac tggtcaaatt
     3361 tacgttgttt cttttccaag ttgcttacca attggcgttg gttatccaga tcgactacca
     3421 gatcgtctaa ctcttgttgc aggcggtttt tcgtcttctc aagtttgtcg tacgcggctg
     3481 ccttttcttc gtactgttga gtcaaattct cgatctcctt ttgaaagcgc ttttttccct
     3541 cttccaatgc ctcaacagtg ctcgcaaaat cttgcagttt ctttttcgaa tcgctcagct
     3601 ggatattcag tgtcgagata tggcgttcca agttctgttt agcttccatc tcttcgtcca
     3661 gctgatcctg aagcgagttg cgctcctctt caagttgacg aagtttagta ctcacgttta
     3721 acttctggcg cgtttcctcc tgcaacagtt cttgggtgtc ttgcagttgc gaactaagtg
     3781 acgctacatc cttcgccagt ttaatggctt tcccttcggc ctcattcagc attccagtaa
     3841 cagactcgac ttcattctga agtttatgta ctttatcatt caactcggcg cgtgcgcgct
     3901 cgccatctga acatttactc tgcaattcct gtacctgcgc ctccagtttt ttctttttat
     3961 gttcaacttc ttgtttcgct tgacccagca cacgaagttc tccagccaag tccgcatttt
     4021 ctttctccag agtttgctta ttcttatcaa ggttcgcctt ggcttccatc tcttcgcgat
     4081 gactgcgatc gcgatcctcg aactcgcggg tgcgacgacg tgcctcttca aacgcttgct
     4141 gggccaaagc atcttcttgt tgcgcgcgtt cctcgtcgaa ctcaagacaa cccatcccgt
     4201 caagacgctg aagatcgatc cagcctttcc aaatgacaca caccccgttt aagatcatgg
     4261 gggccttcaa atatactttc ccggcctcac gctctaagtc tacatattcg cgcgacggcg
     4321 tctgacgctg ctctccttgc caagacgctg ggaaaaattg taatgataaa ttcgtgccag
     4381 tggccacaaa ggcaatctcc gagcgcccgt cgcggcaagc attctggaag cgggcttggc
     4441 gttcttcgtg ggggcgatcg cggaaccccg tgtatttaat ttcgcactca cgcgacagtt
     4501 tacgaaagaa ctcctcgttc tcaaatttcg aacgttgatc tgggacgaca cgtggcattc
     4561 cccgggaagc ggccgcactt cccccggagc ctccgcttcc gcccttgtac agctcgtcca
     4621 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     4681 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
     4741 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     4801 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     4861 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     4921 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     4981 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     5041 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     5101 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     5161 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     5221 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     5281 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatccc
     5341 gggcccgcgg taccgtcgac tgcagaattc ggttcactaa acgagcccgg ttcactaaac
     5401 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     5461 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     5521 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     5581 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     5641 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     5701 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     5761 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     5821 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5881 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     5941 accccgtaat tgattactat taataactag cacgcgttcg aagcttgagc tcggtcccct
     6001 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     6061 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     6121 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     6181 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     6241 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     6301 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     6361 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     6421 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     6481 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     6541 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     6601 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     6661 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     6721 atataattca cttctccaat tgtccctcat atctcctcct tatggtgttt tactaaactt
     6781 ttccatgttc taatcctcat cctgtctact tgccacacaa tcatcacctg ccatctgttt
     6841 tccataatcc ctaatgatct ttgcttttct tcttggcact acttttatgt cactattatc
     6901 ttgtattact actgcccctt cacctttcca gaggagcttt gctggtcctt tccaaagtgg
     6961 atttctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa
     7021 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
     7081 cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgta ctgctgtctt
     7141 aagatgttca gcctgatctc ttacctgtcc tataattttc tttaattctt tattcataga
     7201 ttctactact ccttgacttt ggggattgta gggaatttcc aggtctgaag atcctgctgc
     7261 ttgctgtgct tttttcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     7321 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     7381 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     7441 acagccttct gatgtttcta acaggccagt attaactgcg aatcgttcta gctccctgct
     7501 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     7561 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     7621 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     7681 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     7741 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     7801 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     7861 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     7921 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
     7981 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctcccat ggtaatagcg
     8041 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     8101 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     8161 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     8221 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     8281 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     8341 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
     8401 caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8641 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8701 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8761 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8821 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8881 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8941 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9001 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9061 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9121 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9181 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9241 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9301 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9361 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9421 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9481 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9721 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9841 cgg
//
