LOCUS       sequence-406605-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CD44_PDHX from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CD44_PDHX
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3854..4564,4565..4567,4568..4570))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3896..3917)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4245..4264
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4504..4525
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4567..4576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4652..4855)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4681..4701)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4856..5159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5416..5460)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5645..5878
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6238..6355
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6847..6972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7019..7276
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7278..7657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7688..7792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7793..7861,7862..8653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8011..8030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8824..9093,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaag
     1801 ctaaacggat tgggttttct aaattggcct taaatgattt taaaaaacga gtagcaagtt
     1861 catcgtctac tacgcgtgaa tccgaactca ttgtgactgt aattaattga cgctgctgta
     1921 acttcgcatt gccttcctca tcttcggtca gtttaagtac tgggcggaaa cgacctacgg
     1981 caaggataca cgcttgaggg gggttgatca cagcggtgaa ctcgtcaatt ccaaacatgc
     2041 ccaagttgct gattgagaac gaccctccct ggtactcttc gggcagtaat ttaccgtcac
     2101 gtgccttctt gctcaaagcc ttcactgagt ccgcaatctc ctgaatgcct ttggctgcgg
     2161 catccttaat aatcggagta agtaatcctt tatcagtagc caccgctacg ctaatgtcaa
     2221 taaaaggtaa ttgcttcggg ccttcaccgt cccaactcac gtttacgtct ggcatctgtt
     2281 taagtgtaac agcagctgcc ttgataatga agtcatttac agataccttg atgtcatcct
     2341 tcaccaaatc ttggcgtact ttcaacacgg ctccaaggtc acagtcagcg gtcgcgtaag
     2401 cgtgcggtac agtagactta ctctcggtaa ggcgttttgc aataacacga cgaatattac
     2461 tggcaggaat ctcggtgaag gttccaacag cattgggctg acccggcgtt gacacagggg
     2521 gaattacggg gcgcgggtaa ctcggacccg ctgtagcttg aagtggacta ggcgcagtcg
     2581 gcgtagccgt gggcgcaggt gtagggcgag actcagtaat cttaccggtt tgcttcagtt
     2641 gtaccaattt caaagcgtct tccttggtaa aaataccacg tggtccagtc gctgttccct
     2701 gactggcgtc aagggagtgc ttttcaagga tgttacgtgc tgctggcgac aagcggaaac
     2761 gaagcgtccc agggatgtgt tccttcttca ccgggatcga gatttgtggc tccgggcttg
     2821 gacgcggttc gcttggcttg gacacaggtg ggggcggccc tacatctttc gggatttcta
     2881 catgtttcca atcttcccct tcttctacaa taagtccgat caaagacccc aaacgaatat
     2941 tcttagaccc ttcttccact acgattttgg ccaagatccc gtcatccgac gcatccagtg
     3001 taacgacagc tttgtccgtt tcgatttcgc ataatgcatc ccctgcacta acggcttcgc
     3061 cctccttttt caaccatttt acgatgttgc cttcctccat agtaggtgac aggctaggca
     3121 tcagaatttt aatcgggtcg ctagttgctg gaatgcggtc agtactgtcg gtaatccacg
     3181 gggaatcttc gtctgggatt gggtgaacgg tgctgaaagt atagaaaatg tagccccctg
     3241 acgtagacga gcgctcggat gacgagccgg aactaacgtc gtcgtctgtt ggatttgagg
     3301 gataaatatc ctcggggttg gtgcgatact cccccttttg tacgtaacgc gttccgtcgc
     3361 ggtttacaat tgtaatggtg atgggcccgt cgaaggcatt aggtaaatca gtgacgctag
     3421 tacagtcttc ttcgggtggg gcactagcat tgaaacaata ggtatcgtat tgcgacgtat
     3481 tagaggttaa aatatagaca cctgtgttat ttgccgcgca gatcgaattt gggtgaatgc
     3541 gggggatcac cacatgcccc tcaataaacc catagcgaca cgtctcaaac ccgatcgaaa
     3601 gcgctttttc catttgagcc atcgtcggca aagtgctatt aaaggctttg cataaatccg
     3661 cggcttcggt acgagagatg gagtagcgtc catttttttc gacgtgaaat acacctgcaa
     3721 agcggcacgt aatattaagg tcgatctgag caagtgacag aggaaccaaa cacagacccc
     3781 aggcagcgtg ccaccaaaat ttatccattc cccgggaagc ggccgcactt cccccggagc
     3841 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     3901 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     3961 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     4021 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     4081 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     4141 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     4201 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     4261 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     4321 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     4381 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     4441 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     4501 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     4561 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
     4621 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     4681 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     4741 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     4801 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     4861 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     4921 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     4981 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     5041 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     5101 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     5161 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     5221 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
     5281 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     5341 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     5401 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     5461 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     5521 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     5581 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     5641 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     5701 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     5761 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     5821 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
     5881 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
     5941 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     6001 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
     6061 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
     6121 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
     6181 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
     6241 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     6301 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     6361 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
     6421 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
     6481 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
     6541 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
     6601 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
     6661 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
     6721 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
     6781 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     6841 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     6901 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     6961 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     7021 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     7081 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     7141 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     7201 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     7261 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     7321 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     7381 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     7441 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     7501 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     7561 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     7621 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     7681 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7741 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7801 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7861 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7921 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7981 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8041 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8101 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8161 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8221 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8281 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8341 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8401 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8461 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8521 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8581 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8641 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8701 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8761 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8821 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8881 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8941 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9001 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9061 tgctgccagt ggcgataagt cgtgtcttac cgg
//
