LOCUS       sequence-406612-        9888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CLTC_VMP1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CLTC_VMP1
FEATURES             Location/Qualifiers
     source          1..9888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4649..5359,5360..5362,5363..5365))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4691..4712)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5040..5059
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5299..5320
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5362..5371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5447..5650)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5476..5496)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5651..5954
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6211..6255)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6440..6673
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7033..7150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7642..7767
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7814..8071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8073..8452
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8483..8587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8588..8656,8657..9448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8806..8825)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9619..9888,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 tggtcttctc ctcgctatta agacgttgtt ggatacgctt ggcgtacgac tgcgccattg
     1861 agttaataat cgaaagaata aagtagcaca ccatcacgac caccaacttc tcgaacatcc
     1921 aactcagcca gttctcgccc tggggcgtgc ccatttcgct tttgtggtgt aatttctggc
     1981 gctgggcctc caagtattcc tggaacggct tctgaagtga cggtccaatc cctggaacgg
     2041 cagagttgcg tttctctact tctgccacaa gctcgtcagt agagaactgg ccacgtacta
     2101 ctaagatcag gtttttaatc acgtcctcgg aacagtctac gtcaagaagt cccccaatta
     2161 caactggcaa gcgcgatggg ttgaccttct gcacgtagat ttcaatgtat ttttgaagat
     2221 tattacgata caaataaaga acaagatcat ggacaaaatc aaagcggtca cacacaataa
     2281 ttaagggtaa ctggtctgtc aatttagctt ctttcaggaa atttttgacg cgttctggat
     2341 cgtaacaatt gctctcacga caaatacgtt ccacttcctt gatttgcccc gttttgcaag
     2401 ccgcttggat gtatttaaag tgtacatcgg ggtcttgtga gaaattcacg atactaccaa
     2461 ggaagtaaaa caagccttca aaagacttga atgattcaaa aagttcaata agcgattgtg
     2521 tacttaactg ctcgtgatac ttgcttgcaa cctggacgca aatttgtaag ttttggcgga
     2581 tgttggctga taacatagcg cgaagacatt ccaagctatc ctccacagac agactaccaa
     2641 aatagttcac cagccattcg gggttaagca ggtgcgtatg tactactgcg cgtttaatat
     2701 cgtaaagatc tgtgaaatgc tctaaggcac gctgcagcag accagccttc tcgcacaact
     2761 gcgcgatgtg ggcgcgatca tagtgcgtga acatttgatt accaagaatc gcatcggcga
     2821 cctgcggagc gtgcatcaga ttcatctcca gcaggcgagt ctgcaaaggg ccctcgctgg
     2881 ggcgattgtt tttcagagca tccaacagga aagcggtaca ctgctggata agattgtatt
     2941 ccataaatac gtctacgatc tgggtaatgt ccgccagagg ttcttcatct tgtactaaca
     3001 tttgtgcaaa ttgttgccct tgatcaggag aaatgcgcat aacgttgcgc agcagaaaga
     3061 tccagtcagg cgtatatccc accttcttgg catacagcac aatcttctga acttgccctg
     3121 tttcggcaaa gcactgaatt actttatttg gtacgttcgc acgcaaatat acactcaatg
     3181 caagggttgg atcaactgac ttaacaagat ctcccagttc ttcgctacat tctaacttgt
     3241 cttccttcaa ccacttctcc aaaagttgct tgcggccttg ctgaagcacc gggcgacaca
     3301 gttccagtga ctcgtactta ttcaactgtc cctgatccaa caagatccca aagtattgaa
     3361 gcaacggact cgtttggcct ggctgagcag gcactgattg gaagcgacgg attgtgtctg
     3421 gcgtgcgaag aatccctttc ggtgcgttag ctgcaacctt ggctgcctcg gaataattac
     3481 cctgcgcgaa taaagcattg aacttgcgag cgaaaagctc ctccgcaccc gctaagttgt
     3541 tgcgtacggc catacgcagg gctaaatctg gattctgaag gacgttagtg atgtagggaa
     3601 tgatattttc ttcttcaacg cacacactca gaacttgtcc tttacggttg acaccaatga
     3661 taccggccgt ggcttcatga ggggcagtta caaagatcgt ttcccccgaa atgcggttca
     3721 tatagataca tgtgccggtt tctaagtcgt ataagtgaat atatccgtac ttcgtaatca
     3781 agaagactac atcatgtttc tcgctgattt gcatcgcaac tggaaagtca ttctgagcct
     3841 ctggggggaa gaacacatcc acggctttct tcggaaacgg ctgattgcca gttggaggtg
     3901 tgcccacttc gataatatgt aatttacctc cggcttgtcc acgcacggcg aagcagaata
     3961 acgtgctttc ttctgcgtta ccctccatct tgaactgggc gaatgaagcc gcgtgcccct
     4021 cgataggctg cgatacctta cgatccacgc tgtacagttg cattgctccc acgacgcggt
     4081 tctgttgagc agagatgcca gtcagcagca gccatttctg cttagcgtca gtgcggtagt
     4141 tgatgatttg gcaaccggcc agggaagaat ggcggtcgaa cattttgaca ggttgacttt
     4201 ctccttccat actccagtgg tatacggcgt tgtcggtcac taatgcgacc gtgttcaatg
     4261 aaatccattt ccagaacgta acatcatccg tcatagtatg tgctttcatt ttgctcttca
     4321 tctcgatgtt gaagatttgc agggttttcc cagcctttaa agcaatcact ttcgatgctg
     4381 ggttcatgat tgcgctgtcc gcgctgatcg ggcggcggat cgggttacta ggatcgttca
     4441 tatcaatgat cactacctgc gcctgctccc caactttttc acgaatgcaa atgaatttgt
     4501 cactttccat cgtaagagtg gagaagccga tgtttgctgg attaatacca agattctgca
     4561 attgcaaatg ttcctgaaag cggatgggta aaatctgcgc cattccccgg gaagcggccg
     4621 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     4681 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     4741 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     4801 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     4861 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     4921 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     4981 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     5041 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     5101 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     5161 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     5221 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     5281 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     5341 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     5401 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     5461 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     5521 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     5581 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     5641 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     5701 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     5761 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     5821 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     5881 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     5941 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     6001 actattaata actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg
     6061 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     6121 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     6181 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     6241 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     6301 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     6361 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     6421 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     6481 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     6541 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     6601 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     6661 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     6721 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     6781 ccaattgtcc ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc
     6841 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat
     6901 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc
     6961 cccttcacct ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct
     7021 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     7081 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     7141 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg
     7201 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg
     7261 actttgggga ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt
     7321 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     7381 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     7441 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     7501 ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     7561 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     7621 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     7681 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     7741 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     7801 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     7861 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     7921 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     7981 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     8041 ccactcccca gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag
     8101 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     8161 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     8221 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     8281 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     8341 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     8401 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg
     8461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8521 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8581 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8641 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8701 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8761 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8821 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8881 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8941 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9001 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9061 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9121 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9181 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9241 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9301 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9361 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9421 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9481 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9541 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9721 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9781 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
