LOCUS       sequence-406623-       13653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All in one recombinant baculovirus production vector. Encodes Cas9, 
            sgRNA and donor for homology independent targeted integration in the
            ACTB locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 13653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: MultiMate-HITI-2c ACTB
FEATURES             Location/Qualifiers
     source          1..13653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             635..700
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             707..727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             752..4852
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             752..4852
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4853..4900
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    5163..5297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  5418..5583
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5773..6361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6262..6281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  6496..6719
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6786..7319)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7508..7513,7514..7530,7531..7536))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(7607..7640)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7682..7706
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7966..8670
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(8108..8126)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8376..8395
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8686..8739
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8740..9339
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8740..8759)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    9661..9681
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        9699..9947
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9699..9719
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9870..9889
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9968..10043
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10067..10087
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        10135..10226
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     10151..10168
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        10296..10330
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             10339..10350
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     polyA_signal    10828..10962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    11071..11091
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        11103..11482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11483..11686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      11731..11740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11737..11739,11740..11742,11743..12453)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11737..11739,11740..11742,11743..12453)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12043..12062)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12390..12411
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    12722..12856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    12977..13565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13030..13050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13448..13459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(13581..13605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgcgggcc catggactat aaggaccacg acggagacta
      661 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg
      721 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat
      781 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
      841 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
      901 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
      961 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     1021 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     1081 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     1141 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     1201 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     1261 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     1321 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     1381 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     1441 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     1501 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     1561 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     1621 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     1681 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     1741 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     1801 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     1861 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     1921 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     1981 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     2041 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     2101 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     2161 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     2221 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     2281 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     2341 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     2401 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     2461 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     2521 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     2581 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     2641 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     2701 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     2761 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     2821 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     2881 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     2941 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     3001 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     3061 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     3121 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     3181 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     3241 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     3301 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     3361 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     3421 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     3481 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     3541 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     3601 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     3661 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     3721 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     3781 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     3841 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     3901 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     3961 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     4021 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     4081 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     4141 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     4201 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     4261 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     4321 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     4381 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     4441 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     4501 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     4561 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     4621 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     4681 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     4741 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     4801 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc
     4861 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag gaattcggca gtggataggg
     4921 ggagacccaa gctggctagt ggatcccggt ccgaagcgcg cggaattcaa aggcctacgt
     4981 cgacgagctc acttgtcgcg gccgctttcg aatctagagc ctgcagtctc gacaagcttg
     5041 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     5101 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     5161 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5281 cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc
     5341 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata acccctaggg
     5401 tatacccatc taattggaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     5461 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     5521 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     5581 acaaccggtt gacttgggtc aactgtcaga ccaagtttac tcatatatac tttagattga
     5641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6421 gttctttcct gcgttatccc ctgattgact tgggtcgctc ttcctgtgga tgcgcagatg
     6481 ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa
     6541 ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
     6601 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
     6661 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatgtaaag actatattcg
     6721 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg
     6781 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
     6841 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
     6901 taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc
     6961 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc
     7021 tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca
     7081 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga
     7141 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc
     7201 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca
     7261 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc
     7321 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     7381 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     7441 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     7501 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt
     7561 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgccataa cttcgtatag
     7621 catacattat acgaagttat ctgtaactat aacggtccta aggtagcgag tttaaacatc
     7681 aacaagtttg tacaaaaaag caggctgttt aaacgatatc gcgggactct ggggttcgaa
     7741 atgagacagc tccccacaca ccacagggtg tgtggggagc tgtcacatcc agggtcctca
     7801 ctgcctgtcc ccttccctcc tcagatcatt gctcctcctg agcgcaagta ctccgtgtgg
     7861 atcggcggct ccatcctggc ctcgctgtcc accttccagc agatgtggat cagcaagcag
     7921 gagtatgacg agtccggccc ctccatcgtc caccgcaaat gcttcgtgag caagggcgag
     7981 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     8041 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     8101 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     8161 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     8221 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     8281 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     8341 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     8401 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     8461 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     8521 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     8581 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     8641 tccaccggcg gcatggacga gctgtacaag gaattcggca gtggagaggg cagaggaagt
     8701 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa tgaccgagta caagcccacg
     8761 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8821 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     9001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     9061 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     9121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     9181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccacaac
     9241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     9301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctagg cggactatga cttagttgcg
     9361 ttacaccctt tcttgacaaa acctaacttg cgcagaaaac aagatgagat tggcatggct
     9421 ttatttgttt tttttgtttt gttttggttt tttttttttt ttggcttgac tcaggattta
     9481 aaaactggaa cggtgaaggt gacagcagtc ggttggagcg agcatccccc aaagttcaca
     9541 atgtggccga ggactttgat tgcacattgt tgttttttta atagtcattc caaatgacag
     9601 ctccccacac accacagggc tgtccctctt ctcttatgaa gagatatccc taggcccggg
     9661 caactttgta taataaagtt ggtttaaacg atatcaccga gggcctattt cccatgattc
     9721 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     9781 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     9841 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     9901 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgac agctccccac
     9961 acaccacgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
    10021 aaaaagtggc accgagtcgg tgcttttttt atccctaggc ccgggccaac tttgtataga
    10081 aaagttggtt taaacactag tatcgattcg cgacctactc cggaatatta atagatcatg
    10141 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
    10201 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
    10261 gcggatcccg gtccgaagcg cgcggaattc aaaggttgac agctagctca gtcctaggta
    10321 taatgctagc tactagagaa agaggagaaa tactgaattc agatgctggg atccatgaat
    10381 attaatgact taattagaga aatcaaaaac aaagattaca cagtgaaatt gagtggtacg
    10441 gatagcaata gtatcacaca gctaattatt cgcgttaata atgatggcaa cgagtatgta
    10501 atttctgaaa gtgaaaatga atcaatcgtt gaaaaattca tctctgcatt caaaaacggt
    10561 tggaatcaag aatacgagga tgaagaagaa ttttataatg acatgcaaac aatcacctta
    10621 aaaagtgagt tgaactaacc tacgtcgacg agctcacttg tcgcggccgc tttcgaatct
    10681 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc
    10741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    10801 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    10861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10921 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
    10981 agaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
    11041 gcaataacta tcataacccc taggcccggg caactttgta tacaaaagtt ggtttaaacg
    11101 atgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    11161 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    11221 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    11281 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    11341 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    11401 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    11461 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
    11521 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    11581 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
    11641 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
    11701 agaacccact gcttactggc ttatgaattc gccgccatgg tgagcaaggg cgaggagctg
    11761 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
    11821 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
    11881 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
    11941 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
    12001 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
    12061 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
    12121 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
    12181 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
    12241 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
    12301 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
    12361 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
    12421 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg atagcccggg
    12481 gagacccaag ctggctagtg gatcccggtc cgaagcgcgc ggaattcaaa ggcctacgtc
    12541 gacgagctca cttgtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt
    12601 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
    12661 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    12721 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    12781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    12841 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
    12901 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa cccctagggt
    12961 atacccatct aattggaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
    13021 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
    13081 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
    13141 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
    13201 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
    13261 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
    13321 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
    13381 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
    13441 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
    13501 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
    13561 cccgcatccc taggcccggg agacagcttt cttgtacaaa gtggttgata agcttaaaca
    13621 ctagtatcga tcgcgatgta cgggccagat ata
//
