LOCUS       sequence-406626-        9165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CSF2RA_CRLF2
FEATURES             Location/Qualifiers
     source          1..9165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3926..4636,4637..4639,4640..4642))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3968..3989)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4317..4336
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4576..4597
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4639..4648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4724..4927)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4753..4773)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4928..5231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5488..5532)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5717..5950
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6310..6427
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6919..7044
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7091..7348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7350..7729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7760..7864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7865..7933,7934..8725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8083..8102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8896..9165,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaaa
     1801 gggccacgta cgagcgatca ttcatcacga aagtgaaccc cccgatggtt actacatctc
     1861 cgccttgcaa cggttggtgg ggcaattgca acgacccgcc gctggcttcc ttttcttctg
     1921 tttgggggtc caacatgcga ggactttcag cttcagtctt tgccagttgg acgaccaaag
     1981 gttcctcagg gcccgactcc tgttcggcgc ccgccatctt gtgcagatgg gccacattct
     2041 gtgtatcggt aatccattct tgaaagtttc cctggtgaat ctcgaacagg ccagggaaga
     2101 tactttttgg atcaggtacg cttgggatta aaaatttttt cacacgccac aacttccaaa
     2161 gggacaataa aagcagtgat accattaaca gaatagctaa gctagagata aggataaact
     2221 ttgacaactt tggctttggc ggcgtagggg tttcggcgca agcgtcgcgg atttcgccgc
     2281 gttgccagca tgttacttca gaccagtcgc tggggtaggt atcgggcccg taaacatctt
     2341 ccattgcttt gacacgaacc caaaaactat aacacttctc cgcatctaat ccctcgatgg
     2401 tcacattgca agtattttct tgtttgcttt gccactccgt atcaaaaggg gaacgatatt
     2461 gcacctcata caacaagtcg ccgtaactaa gatcagagca agtaacggtt acggcatcct
     2521 gatgccaaga aaaacggacg tgcttggggc ttgacggttt caggtaatat accatccagc
     2581 gcgaagcggt gaagactgga tgtgtcccgt tgcgaataga gaagtaaaga atatcgtcgc
     2641 gttgttctgc gtccaacagg caaccactcg tatgaccctc ctgcagaagg tagttcgtgc
     2701 attggtcgta tgcctcatcg ccattaaagc ggtaatgaaa cgttaaatta gtgcgagagt
     2761 atttcgaggc gttccacgta acttgtacgg tttccagatt aaagtaaatg atctggattt
     2821 gtactccctc cgctggtaaa cgcaggcagc taaagcgttt aagacggggg gacggcggtt
     2881 gcaagctacc cagattatgc cactgaactc ctgcttgcgt tacggagtgc gaaccaaact
     2941 caatcgcctc tgaccaagaa ctccagttta agatgcgcac atcggcagca cggattttta
     3001 ccgagtgttt agcacgtggc tcagagctgg gaaaattata gcgattttca agatcgccgg
     3061 aaacattgat caataagttt tcggttccgg gttgcgtgtt cttgcggtgc acgtccaatt
     3121 ggtattggaa atcaagatat gacaatttct ggtacgtgcg tggttgtttc cagcggacca
     3181 ggcagtgagt tgtattgcag cgtactgtaa cgttagatgg aggattgaag cgttcgattt
     3241 ttttagtgtc cagaagagag tcaaagaact gaataccgat ttcgcgtgat gtcccgttta
     3301 ctaagaaata gttgcgagag gtaagccccg ataaattgtc cagatggcac ccaacgtgcg
     3361 tgccactgtc ctggatataa taggggcagc gaatctcgcg acggcgcttg gaattacgga
     3421 tataaaggaa atactgtacg tcacggggtg cagtgggtcc gcgtgcccag gtgcagttca
     3481 ttaaatctgc attatagatg aaacagctaa aattttgggc ggcagtccct tcacggccag
     3541 agttgggata cagcagcttc tgttgaaaac cacgttgact tgtgttgacg tgaacttcaa
     3601 aagtcacccc ttcgtgaaga cagatctcgc gaaaggtaca agaacactca ttgttcgata
     3661 aacgcggctc tacaacacga ttctttttgt ctgtcaagaa acacttgctg aaggtcgtgt
     3721 tttcttgaca atcccacgat aagttcatcg tgcgactatc gaagcgcacg tttaagctcg
     3781 atgctggggc taccgtacgc agatcggact tttccgggat taataagaag gcaggatgag
     3841 gaagctcgca caataataat gaggtcacca acaacaacat tccccgggaa gcggccgcac
     3901 ttcccccgga gcctccgctt ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     3961 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     4021 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     4081 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     4141 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     4201 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     4261 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     4321 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     4381 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     4441 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     4501 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     4561 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     4621 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
     4681 actgcagaat tcggttcact aaacgagccc ggttcactaa acgagctctg cttatataga
     4741 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     4801 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     4861 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     4921 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     4981 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     5041 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     5101 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     5161 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     5221 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     5281 attaataact agcacgcgtt cgaagcttga gctcggtccc ctcggggttg ggaggtgggt
     5341 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     5401 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     5461 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     5521 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     5581 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
     5641 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     5701 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     5761 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     5821 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     5881 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     5941 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     6001 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     6061 attgtccctc atatctcctc cttatggtgt tttactaaac ttttccatgt tctaatcctc
     6121 atcctgtcta cttgccacac aatcatcacc tgccatctgt tttccataat ccctaatgat
     6181 ctttgctttt cttcttggca ctacttttat gtcactatta tcttgtatta ctactgcccc
     6241 ttcacctttc cagaggagct ttgctggtcc tttccaaagt ggatttctgc tgtccctgta
     6301 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     6361 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     6421 tttaaaattg tggatgaata ctgccatttg tactgctgtc ttaagatgtt cagcctgatc
     6481 tcttacctgt cctataattt tctttaattc tttattcata gattctacta ctccttgact
     6541 ttggggattg tagggaattt ccaggtctga agatcctgct gcttgctgtg cttttttctt
     6601 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     6661 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     6721 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     6781 taacaggcca gtattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     6841 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     6901 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     6961 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     7021 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     7081 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     7141 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     7201 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     7261 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     7321 ctccccagtc ccgcccaggc cacgcctccc atggtaatag cgatgactaa tacgtagatg
     7381 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     7441 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     7501 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     7561 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     7621 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     7681 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
     7741 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7801 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7861 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7921 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7981 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8041 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8101 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8161 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8221 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8281 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8341 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8401 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8461 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8521 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8581 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8641 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8701 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8761 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8821 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8881 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8941 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9001 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9061 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9121 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgg
//
