LOCUS       sequence-406629-        9166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin
            reporter. Can be used to generate recombinant baculovirus 
            particles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: MultiMate-Cell-cycle
FEATURES             Location/Qualifiers
     source          1..9166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      629..638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             635..1312
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             635..1312
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             1343..1672
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     polyA_signal    1696..1817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1836..1856
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        1896..2199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2200..2403
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2459..3109
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     primer_bind     complement(3501..3518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3507..3731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3751..3771
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3798..3818
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    4005..4053
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4078..4097
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(4189..4298)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    complement(4329..4353)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        4402..4781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4782..4985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5043..5420
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      5433..5442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6640..6774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  6895..7060
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7250..7838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7739..7758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  7973..8196
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8263..8796)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8985..8990,8991..9007,9008..9013))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(9084..9117)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(9159..9166,1..17)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtacaaaaaa gcaggctgtt taaacgatat ccccatatat ggagttccgc gttacataac
       61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      541 tctatataag cagagctgga attctgcagt cgatcgacgg taccgcgggc ccgggccact
      601 agtaacggcc gccagtgtgc tggaattcgc caccatggtg agcgtgatca agcccgagat
      661 gaagatcaag ctgtgcatga ggggcaccgt gaacggccac aacttcgtga tcgagggcga
      721 gggcaagggc aacccctacg agggcaccca gatcctggac ctgaacgtga ccgagggcgc
      781 ccccctgccc ttcgcctacg acatcctgac caccgtgttc cagtacggca acagggcctt
      841 caccaagtac cccgccgaca tccaggacta cttcaagcag accttccccg agggctacca
      901 ctgggagagg agcatgacct acgaggacca gggcatctgc accgccacca gcaacatcag
      961 catgaggggc gactgcttct tctacgacat caggttcgac ggcaccaact tcccccccaa
     1021 cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga agatgtacgt
     1081 ggaggacggc gtgctgaagg gcgacgtgaa catgaggctg ctgctggagg gcggcggcca
     1141 ctacaggtgc gacttcaaga ccacctacaa ggccaagaag gaggtgaggc tgcccgacgc
     1201 ccacaagatc gaccacagga tcgagatcct gaagcacgac aaggactaca acaaggtgaa
     1261 gctgtacgag aacgccgtgg ccaggtactc catgctgccc agccaggcca agggatatcc
     1321 atcacactgg cggccgctcg agatgaatcc cagtatgaag cagaaacaag aagaaatcaa
     1381 agagaatata aagaatagtt ctgtcccaag aagaactctg aagatgattc agccttctgc
     1441 atctggatct cttgttggaa gagaaaatga gctgtccgca ggcttgtcca aaaggaaaca
     1501 tcggaatgac cacttaacat ctacaacttc cagccctggg gttattgtcc cagaatctag
     1561 tgaaaataaa aatcttggag gagtcaccca ggagtcattt gatcttatga ttaaagaaaa
     1621 tccatcctct cagtattgga aggaagtggc agaaaaacgg agaaaggcgc tgtaatgaat
     1681 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1741 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1801 ctcatcaatg tatcttagat atccctaggc ccgggcaact ttgtataata aagttggttt
     1861 aaacgatatc ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1921 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1981 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2041 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2101 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2161 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2221 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2281 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2341 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2401 gctggaattc tgcagtcgat cgacggtacc gcgggccctc cgcggccgcg ggtcccccat
     2461 ggtgagtgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg
     2521 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat
     2581 gacactacgc gtcacaatgg ccgagggcgg gccaatgcct ttcgcgtttg acttagtgtc
     2641 acacgtgttc tgttacggcc acagagtatt tactaaatat ccagaagaga taccagacta
     2701 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg
     2761 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc
     2821 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga
     2881 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac
     2941 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caaatgaaga ctacttacaa
     3001 ggcggcaaaa gagattcttg aaatgccagg agaccattac atcggccatc gcctcgtcag
     3061 gaaaaccgaa ggcaacatta ctgagcaggt agaagatgca gtagctcatt actcaatgtt
     3121 gccttcccag ggcggatatc catcacactg gcggccgctc gagcccagcc ccgccaggcc
     3181 cgcactccgc gccccgtcct ccgctaccag tggcagccgc aagcgcgccc gcccgcccgc
     3241 cgcccccgga cgcgaccagg ccaggccacc ggcccgcagg agactgcggc tgtcggtgga
     3301 cgaggtttcc agccccagta cccccgaggc cccagacatc ccagcctgcc cttctccggg
     3361 ccagaagata aagaaatcca ccccggcagc aggtcagccg ccccacctga catccgcgca
     3421 ggaccaggac accatctaag gggaattcct cgagaagctg ccgctcgagt ctagagggcc
     3481 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3541 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3601 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3661 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3721 gggctctatg ggatatccct aggcccgggc caactttgta tagaaaagtt ggtttaaacg
     3781 atatccctag gcccgggcaa ctttgtatac aaaagttggt ttaaacgtac ccgtagtggc
     3841 tatggcaggg cttgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     3901 gggttggggt ggggaaaagg aagaaacgcg ggcgtattgg tcccaatggg gtctcggtgg
     3961 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     4021 acccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     4081 tctccttccg tgtttcagtt agcctccccc atctcccggt accgcatgct atgcatcagc
     4141 tgctagcacc atggctcgag atcccgggtg atcaagtctt cgtcgagtga ttgtaaataa
     4201 aatgtaattt acagtatagt attttaatta atatacaaat gatttgataa taattcttat
     4261 ttaactataa tatattgtgt tgggttgaat taaaggtccg tatactagta tcgataccta
     4321 ggcccgggag acagctttct tgtacaaagt ggttgataag cttaaacact agtatcgatc
     4381 gcgatgtacg ggccagatat agacattgat tattgactag ttattaatag taatcaatta
     4441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     4501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     4561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     4621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     4681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     4741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     4801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     4861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     4921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     4981 gagctctctg gctaactaga gaacccactg cttactggct tatgtaccgc gggcccgccg
     5041 ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga
     5101 ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag agctattcca
     5161 tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcca
     5221 tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc
     5281 gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc
     5341 gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact aaggccatca
     5401 ccaagtacac cagcgctaag gatccaccgg tcgccaccat ggccgaaggg agcgtggcaa
     5461 gacagcccga tctgctgaca tgcgacgacg aaccaatcca cattccagga gctatccagc
     5521 ctcatggact gctgctggct ctggccgctg acatgactat cgtggcaggc tctgataacc
     5581 tgccagagct gaccgggctg gctatcggag cactgattgg ccggtccgca gccgacgtgt
     5641 tcgattctga gactcacaat agactgacca tcgccctggc tgaacctggg gctgcagtgg
     5701 gagccccaat taccgtcggc ttcacaatgc gaaaggacgc tggctttatc gggtcctggc
     5761 accgccatga tcagctgatt tttctggagc tggaaccacc tcagagagac gtggcagagc
     5821 cacaggcttt ctttcggaga acaaacagcg ccatcaggcg cctgcaggcc gctgagactc
     5881 tggaatccgc ttgcgcagcc gctgcacagg aagtgaggaa gatcaccggg ttcgatcgcg
     5941 tcatgatcta cagattcgcc tcagacttta gcggagaagt gatcgcagaa gatcgctgtg
     6001 ccgaggtcga aagtaaactg ggcctgcatt acccagcctc aaccgtgcct gcacaggcac
     6061 gacggctgta tacaatcaac cccgtgcgga tcattcccga cattaattac agaccagtgc
     6121 ccgtcacacc tgacctgaac ccagtgactg ggaggcccat cgatctgagc ttcgccattc
     6181 tgcgcagcgt gtcccctgtc cacctggagt ttatgaggaa tatcggaatg catggcacca
     6241 tgtctatcag tattctgaga ggcgaaaggc tgtgggggct gatcgtgtgc caccatcgga
     6301 caccttacta tgtggacctg gacggcaggc aggcttgtga actggtggct caggtgctgg
     6361 cttggcagat tggcgtgatg aaggaggaat aataagggga gacccaagct ggctagtgga
     6421 tcccggtccg aagcgcgcgg aattcaaagg cctacgtcga cgagctcact tgtcgcggcc
     6481 gctttcgaat ctagagcctg cagtctcgac aagcttgtcg agaagtacta gaggatcata
     6541 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     6601 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     6661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     6781 ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     6841 tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag
     6901 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     6961 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     7021 ttccacccct cccagttccc aactattttg tccgcccaca accggttgac ttgggtcaac
     7081 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7381 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7921 attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg ggtgtgggcg
     7981 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     8041 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     8101 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     8161 gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa
     8221 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     8281 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     8341 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     8401 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     8461 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     8521 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     8581 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     8641 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     8701 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     8761 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     8821 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     8881 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     8941 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     9001 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     9061 ccttgggcag cagcgaagtc gccataactt cgtatagcat acattatacg aagttatctg
     9121 taactataac ggtcctaagg tagcgagttt aaacatcaac aagttt
//
