LOCUS       sequence-406631-       13674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 13674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CUX1_NUTM1
FEATURES             Location/Qualifiers
     source          1..13674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(8435..9145,9146..9148,9149..9151))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8477..8498)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8826..8845
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9085..9106
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9148..9157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9233..9436)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9262..9282)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9437..9740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9997..10041)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10226..10459
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10819..10936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11428..11553
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11600..11857
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        11859..12238
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12269..12373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12374..12442,12443..13234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12592..12611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13405..13674,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatt
     1801 ggctacggcg acgcttcttg cgacggcccg tcacgaagct atcacaacgg cgtttacgag
     1861 gctgactagg acgcacaaca ccgagtgcca gcggtttctc tctcggaaca cccagttgcg
     1921 caccgctatg cggggtcttt tcagtaggcg ctggaccgcc agccaatgca cgtttgccgc
     1981 tcttcgccac cgggtacggc aattttgacg gacccttggc gcccgccggt aacaggtggg
     2041 aggccctctg gctgccctga ccaccgcttg catgatgcgg gctcagcggg tgctcacgcg
     2101 gactgaggga cagcttgctc gccagcaggt acgcgaagtt ggacagttcc tcgtcctcct
     2161 cttccgcttc cttctttttc ttctccttgc ctttcgcacg atcagcggaa cggtgcgttt
     2221 tgctgacgct ggtctctcgg ggcacaatcg cgttgcgggt gcctcggcgg gggagcgtag
     2281 agcccaatcc ctggtgggaa gacgtcgctt ttggagtccc ggtggtatag ctttcctgtg
     2341 actcctgcag cggtgccaga ttcttcggtt tggacagatc cgggtccacc ctgccctcac
     2401 cctgcgggtc gtaggaatgc acgttcagcg gacaggtgtc ttcgctaacg cgaagctgaa
     2461 gaccacagtc gtctttaacg tccaggatat taaccggttc aatcgtttct aacagagggc
     2521 tgaaggaatt acctcgtgat cccgcttctt ggctagcctc aggcaagctg ctcgcgtcct
     2581 cagtacccat gatcagcaga ttgccctggc acgtaggagg cagagtctct ttgctggaac
     2641 ccagggtaga gtcgcctgat tccagcagcg gaaagcagcc ctctgcccac agatcggacg
     2701 gatgacccac tgtttcgcaa ctttgttcct ggttctcctt agaacgtagc ggtgggctgt
     2761 tcgcgcgcag gggccccggt aagctgcagt aattacgttt ctccaaggca gcagccgcca
     2821 cggtaccacc gcacgggatc acggatgatt cggtgtcgcc agggaccagg gtctcacctg
     2881 ggcccaggct aatattacca cggctgccca aaccctggca gccctcctgg tactcggtca
     2941 cgcacttctc cacctggaag ctctcgataa cttcttcgat ctgaacggga agctccagac
     3001 ctaccgcatc catctcgcta gacagccaaa cgcccgggct cagacagaca tcatcgcgct
     3061 cgcctgcggc tctggggctc gggttctgat cgtaagattg cgccatcgga gtaccacggt
     3121 cgtcacccca catggcgcca gagctaccct cccacgggac cgcgagcggc tctttgccct
     3181 gcggcagcac accccgacca ccggtctgct gacccttctg taaccctaga acttgtttct
     3241 ccaggccctg accctggagc ggcgccagct ctgctagacc cgcatctagg gacggggtgc
     3301 tttccggctg aaaacctccc tgccagcata gcggcaatgc ctcgctggta cggtcggcga
     3361 ctagacagtg acctggcggg cccgcgccgc ccaggtgacc atcctggtga aggctcacct
     3421 ggttgataac cttaccggaa ccgcgtctct ctacacccag cggacccggc gtaccttgtg
     3481 gagccgctaa ttccggttgc agatcccaag agcttggact tggcagagtg ttgccatcac
     3541 gatgcatgcc acgcgggctg tctaatgctt gttcctgacc gccgtgaacc tcgcgagcac
     3601 gtttgccgga ggagctaact ttacccagac acgcagcgcc acccgcgcct tgcaaccccg
     3661 gggacggacg cagacggccg tcaccgtctt cgtcctcaac actgccggac ggggagctat
     3721 ccagttgtgc gccgctgaag gacggcgggg cctcggcgtc ctcttcttcc tccaacgcca
     3781 tcaatctttt ctgcaccagt tgagctaggg tcaaaccctc ctcttgttca agttcttcga
     3841 tgagtgcgag aggatcgcgc tgcttttctg gactcagtag atctgccagg aactgcggat
     3901 ggatcactgc ttccaccttg gaaacaaaga ccttctggct gcacagttcg ttaatgtaac
     3961 ttagtaaccc cgggtccgga tacatacctt cttcctcttg ttgctggcct tcctcctcct
     4021 gcttgccgtc gctttcacct gtcgccaggt gcgtaccgac aagccattcc atgatgtcaa
     4081 cgtattcctt caccgcctcc ggcggaatct ccttcggcgc ctccggagcc ggcggacgct
     4141 gcgctttacg ctggcggcga cgtggcgcac gggttttgct tgccgctttt ttcgggatgt
     4201 aaaccggttg ttggcacacc tcgcttgcca gcggtcccaa agggtccagc ttaagcgggg
     4261 tcgctgggct cagaccttgg gagccattca tgagctgggt gttttggatt tgcatctcct
     4321 ccgcttcaaa ttccataaaa cgttctgcca tttcgtaaaa gatcatgcga tcgaagttgc
     4381 tggtgtgttc ccattcttga acagcgagcg gcaggccttc ctccagggtc atggttggtt
     4441 tcagacgtgc taaagagcgc agcaccgggc tatagttgtg aaaccagtta attacagtag
     4501 aagtcttcag attcagctga gttgccaaat cctcgatcgt tttcgggctc gggtagggtt
     4561 tttgttgata tgcgcgtttc agggcttcct tctcttccgg cgcgaggacc acacgcggct
     4621 tctttaattg atgctgcggt tgcgggctcg caccctggct atactcggtg cctacactcg
     4681 gcggttcgca cggctggctg tcgctaacgc tggaatgacg tctcttcata tacgccttct
     4741 tttccatccg tttcatgtcc attaacttct ccacgttatt cggatcgttc agccaaagct
     4801 gcatacgcac gaacggttca cgacccttta aggacagttt atgccacggc ttcgggcgcg
     4861 ccagcaggtc ggatacgcta ccttgagtca gacctaaaat ggtttcacca aacaggcgct
     4921 gacccaggtt gttatcggtc agaacctctt taacacgttt ggtaatacca taggtgtcca
     4981 gttccgggct catggcgacc agctcttgaa ttgaaagtgc actgtggccg gatagcggga
     5041 gcggagtagt cggttggctg tcagacgccg gcgggtcgct cggctccggc ggtttgctgt
     5101 ccttgctcgc ctcaatcggc gggcatggtt gctgcaccaa ctcagtcagg ctcttcacag
     5161 actctgagga actcatcggg gattcaggag ccgggctgca tgacgctgag gttttcgggg
     5221 tggattcgga ggaaacgcac ccttgttgta gcgggtcctg gagagaggtc accgagtgca
     5281 agacgggacc ttgttgctga ccctgaaccg gcaacacgcc ttgacccaat tcgccgttca
     5341 gccacagctg catgcggatg aacggttcac ggcctttttg ggtcagcttt gaccaaggtt
     5401 tcggacggga cagcatatcc gaaactgatc cctggctcag acccagcacc ttttccccga
     5461 agatgcgctg gcagatgccg ttttttgcca gcttctcttt aacctgacgc gtcaattcga
     5521 tcgtatcaac ctcctgatac atgtaaactt catattgttc cggggtcagc ggcggcacgc
     5581 tcggctttgt cggcttgctc atcggcacga tgggagagct cgggagcggg ctattctgcg
     5641 gggtttcgct acggctcgcg ccggtgagag acagttcgct cgactggctg taggggcttt
     5701 cagctgaagg ccactctttc cagtactcac tgctagaagg accttgcaac tgggatctct
     5761 ccgcacgggg ctggcttccg ccaccgctac cgcctgagcc cttctcctta ccaccacccg
     5821 tctcttctgc cttcgcctcc tcgctagacg ccgcgttacg acgttctggt tgaaccgcgc
     5881 tccaccaatg atctttccac gcaccgctac gacccacttc attcttcacc tgacgcaaca
     5941 caccctgagc acaatccgca gcgccctgcg ggtccagtcc aggagcgtct tgggcttcct
     6001 tctttaatgc cggcggattc ggcagcgcgg acgcacccgc ctccggggca gcgctcggtt
     6061 ttttgaggga aatcgccaaa ggcgggtagc tgctaacagt cggcatcggg gaggtgctca
     6121 acaatttcgg cgtcaagatg gtaatgtcgg attgcgacag cggtgcctgc tttagagccg
     6181 ggtccagcgc cgcttgctgg gcttccattt cacgacgggc ttgttgcaga atactacgaa
     6241 tagcgtcatc actgttaccg gagccagatg cactcgacgg ttgtgccggc tctgccgtct
     6301 tctgaacttg aagctcacgc tttgcctgct ccaggatgct tttaatggcc tcgtcgctac
     6361 cggtttcgga agcgcggatt ctggtggtaa tgttaccttc agaaccgtta cggcgcttcg
     6421 gtacctcctg aaacaggcgg ttcaaactct ggcccggatt ctcacgctga cgtccctgga
     6481 tgcttctcag cgccagaatg ttttgctcgt cgcttaagaa ctgtttcatt ttgtggaacg
     6541 gctccttacc acgaactgtc agcttattcc acggtttcgg ccgtgccagg atctcgctta
     6601 cgctgccttg ggacagaccc agtacataat gaccaaaaat acgctgacca atattgtgtt
     6661 tgatcagctg ttccttaact tgacgtgcta tttcggcggt gtccatctct tcgccctcag
     6721 acacacttcc tgcgctctcg ctttggctag gagacggcgc cataccatta acatcggggg
     6781 actgttggag cgggctctgg ctggaaatgc tgttggtgga gtacggaccg gtgctgaaaa
     6841 tcattgaggt actaccagcc tcctgcatgg ccttagaata gaagctctgc atcagctgtc
     6901 gttggagcag gctgttcaga gcaaatttac cggtagatgc cagcggcaag ctcggcgacg
     6961 ccagagagct gctaaagaaa tcctggctca ggcctgccgg gctaaattgg tgggtaccat
     7021 tggtattgct cgcctgctcc cccggattac gtggcagctg ggacggcggt ggcgccggca
     7081 ggctacccgg acgacggctc tctggctggt ccttgccctt gcgacgtgcg ctgccggaca
     7141 ggtctgagtt ggagatgcgc agagctgcgt tttcggactg cagtgaacgg tttttctcca
     7201 gcagcagaac ctcaaggggt ttggcggcgt cctgtgtccc tgcaccctcg cttggcgcga
     7261 actccatcga cttcaggata ttcagctctt ttttcacctc ttcataatcc gcttggccct
     7321 tcagcttttc ctctaactgc tttagggtgc tgttcttcgc cgagagctgt tgttccaatt
     7381 gagagatttg actcgcagag ttctcgcgca gcttggtcaa gctcgcctgc agacgctgca
     7441 catcttcgac cagttgtgcg atttcgcgtt ctttagcggc aagttcaact tcaaggctgc
     7501 tacgggtgag aacctcgatc gcctgttcaa cgtccggagc cttctgtatt tgagaagcta
     7561 attgtaaact atgattcgcg ctagacagtt gttcacgcaa tgtctccgct tcgcgctggg
     7621 ccacttcggc tcgctgattt gcgcgttcca aatcggtcat aatcatctcg atctcatcgg
     7681 cctttgcggt ggtttcttca tcatatttgg tcttcaaatc gaacaattcg gtgcgggttt
     7741 tctccaaagc cgtttgaagc gattgaactt tgtgttcagc ttcctccagc ttggatgtcg
     7801 tagacatctg ggtttcttgc agtttgcgct ccttctccgc gaaatcgttt tgcagcttct
     7861 gctccttctc cagagcgatt gtttcggctt ggttcttcag cgtctgttca tattcacgga
     7921 tcttctcttt cagcgctttg atggtaactt cttggttttt aacctcggcg aactctttat
     7981 tgtattcctc cagggtttca cgtaatttct ggttctccgt ttcaatatcg tgcagacgct
     8041 gcactttcag ttgcaattgt tggcccaggt ccagcgccgg aaccgggtcc gggacatcga
     8101 tcagacgttt gtaaacattc agaaacgcgg cttcagcttc cttagaacgt ttacttaagg
     8161 catcgatttc gccctggaaa ctcttcagca gcggtgcgac ctgtttgcgc aggtcctcag
     8221 gggtgttttt tttgaactca cgactttgtt cgatcagacg cttacgggat tgctcgcttt
     8281 cgtcctgacg attggccaat accgtggcgg tcgcgtccag ctcccgctgc agttgttgca
     8341 gatcaaagcg tttccagtat tgaaacattg atcctacatt agctgccatt ccccgggaag
     8401 cggccgcact tcccccggag cctccgcttc cgcccttgta cagctcgtcc atgccgagag
     8461 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     8521 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     8581 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     8641 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     8701 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     8761 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     8821 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     8881 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     8941 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     9001 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     9061 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     9121 cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg
     9181 gtaccgtcga ctgcagaatt cggttcacta aacgagcccg gttcactaaa cgagctctgc
     9241 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     9301 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     9361 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     9421 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     9481 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     9541 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     9601 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     9661 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     9721 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     9781 ttgattacta ttaataacta gcacgcgttc gaagcttgag ctcggtcccc tcggggttgg
     9841 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     9901 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     9961 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
    10021 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
    10081 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
    10141 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
    10201 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
    10261 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
    10321 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
    10381 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
    10441 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
    10501 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
    10561 acttctccaa ttgtccctca tatctcctcc ttatggtgtt ttactaaact tttccatgtt
    10621 ctaatcctca tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc
    10681 cctaatgatc tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac
    10741 tactgcccct tcacctttcc agaggagctt tgctggtcct ttccaaagtg gatttctgct
    10801 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
    10861 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
    10921 cccttttctt ttaaaattgt ggatgaatac tgccatttgt actgctgtct taagatgttc
    10981 agcctgatct cttacctgtc ctataatttt ctttaattct ttattcatag attctactac
    11041 tccttgactt tggggattgt agggaatttc caggtctgaa gatcctgctg cttgctgtgc
    11101 ttttttctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
    11161 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
    11221 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
    11281 tgatgtttct aacaggccag tattaactgc gaatcgttct agctccctgc ttgcccatac
    11341 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
    11401 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
    11461 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
    11521 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
    11581 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
    11641 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
    11701 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
    11761 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
    11821 ggctcgccac tccccagtcc cgcccaggcc acgcctccca tggtaatagc gatgactaat
    11881 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
    11941 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
    12001 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
    12061 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
    12121 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
    12181 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga
    12241 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    12301 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    12361 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    12421 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    12481 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    12541 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    12601 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    12661 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    12721 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    12781 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    12841 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    12901 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    12961 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    13021 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    13081 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    13141 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    13201 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    13261 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    13321 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    13381 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    13441 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    13501 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    13561 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    13621 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgg
//
