LOCUS       sequence-406641-        9327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, DDX3X_MRAS from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_DDX3X_MRAS
FEATURES             Location/Qualifiers
     source          1..9327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4088..4798,4799..4801,4802..4804))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4130..4151)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4479..4498
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4738..4759
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4801..4810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4886..5089)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4915..4935)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5090..5393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5650..5694)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5879..6112
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6472..6589
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7081..7206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7253..7510
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7512..7891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7922..8026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8027..8095,8096..8887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8245..8264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9058..9327,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcata
     1801 aaataacgca ctgtaatttg tgcgtgcctg tcgcacggtc gccacgccac tttgtcttct
     1861 tcttcttctt ttggcttttt tcagggattt gctggcggat aacgcgcact aaatcatgga
     1921 aggccttatc gacatttaat ggcggatctt tcgcgctggt ctcgatatat ggaatattgt
     1981 gttttgtggc catttccttg ccctgttcac gcgtgatttt acgtaaatgc atcagatcga
     2041 ctttattggc cactaagatc atggggaacg attcacgatc cttgacacgt aagatcaact
     2101 gatggaaacg atccacatgt tcaaatgagg ccttgtccgt cactgagtag acgatcagaa
     2161 acccatcccc ggtgcgcata tactgctcac gcatcgcaga gaactcctcc tgcccagcag
     2221 tatcaagcac atctaaaatg gcccattggt tatcaatctc cgtacggctc gacgatttgg
     2281 agcggccacg actggagcct ttgtagtgat gttcataagc catattttct aaccacgatg
     2341 ggacctcctg cttcgcctcc acaagcaggt ccaaaagatc cttagtgatg ttaatattac
     2401 gttcattaaa gaagctagta gcaagaccca aatttcccac gcgtcctgta cggccaatgc
     2461 ggtgcacata ttcctcaatg tcagacggaa gatcaaaatt aattacatgt tttacattcg
     2521 aaatgtccag tccacgggcg gctactgcag ttgcaaccaa aatggggctt tttccagaac
     2581 ggaattggtg taaggcctct tcgcgatcgc gttgcgaacg gtccccatga atacttgtac
     2641 aggcgtatcc ctcatgataa aggaagtctt ccaaggaatc ggcccccttt ttggtttcta
     2701 caaatactaa cgtcaaactg tctttacccg tcgcatttaa aagatccaaa aggaaactgc
     2761 gcttatccga ctcctccacc caaactactt tttgcgtaat gttctcagac gtacttccga
     2821 cacgacccac agccaaaaag atatactcgt ctaagaagtc gcgggccaac atttggattt
     2881 ctttggggaa agtcgcggag aacatcatgg tatggcgtac gcccttgggg ggcatcgtgt
     2941 cctgttccac gatacgacga atttggggct cgaaccccat atcaagcatg cggtcggctt
     3001 cgtccagaac cagatattta cagaaatcca gaccaatctt tccacgttcc atcatatcga
     3061 ccaggcgtcc aggggtagca accaaaaggt ggcagccgcg ttctaaatcg cggatttgct
     3121 gtccaatatc ggcgcccccg tacaccacac aagggcgtac acgtgaacga taactgaatt
     3181 tacgggcttc ttcataaatc tggactgcaa gctcacgagt aggcgccaaa acaagggaga
     3241 tagggtattg cttacggcga ccataacgcc cattttcctt catagcacga agtgcttcac
     3301 cggggccgtc ggagtaaatt tgcgacagaa tgggcagcaa aaaagcggcg gtcttcccag
     3361 aaccggtttg tgcacaggcc attaagtcgc gcttttcctt gataatcgga atagcgtgct
     3421 tctggactgg cgttgggcga gtgtaacgag tcagttcgat gtttcccata ataatctctc
     3481 ccatttctac gtctgagaaa ctttcaatgt ggggcgggca attgttcccc gttgcctcga
     3541 cgggaatgtc gtcatacttt tcaaaattaa tcccggtgtt gcctcccgaa aacagctctt
     3601 gctctaagcg ttcgctagga ggaagtggct tactccaatc gtcctcgtct gatttgtcgc
     3661 accaacgaga gttgccgccg cgctcgaact taccgaatcc cgagcggtct ccgcgggagc
     3721 caatgccgtc gtagtcggag cgtccacgat cgtcgaaacg tccacgtgag ccagatccgc
     3781 gatcagaaaa gaagctcgac ttcccgcgtg agtctgagcg ggacccgaag gaagagtatg
     3841 catccttatc cttcgaactc gaccagccgg agctatcctt atcgtaaaag cccttcgttg
     3901 cctcacggtt gcgcagatga ggcggaatgt agcggccttt gctcgcggtg gaacctccac
     3961 tttgattatc actagaatta aggtccagcc cagcaaattg ctggtctaac cctaacgcat
     4021 tttccaccgc aacgtgagac attccccggg aagcggccgc acttcccccg gagcctccgc
     4081 ttccgccctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     4141 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca
     4201 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     4261 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     4321 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     4381 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     4441 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     4501 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     4561 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     4621 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     4681 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     4741 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     4801 ccatggtggc gaccggtgga tcccgggccc gcggtaccgt cgactgcaga attcggttca
     4861 ctaaacgagc ccggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc
     4921 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     4981 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     5041 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     5101 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     5161 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     5221 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     5281 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     5341 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     5401 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagcacgcg
     5461 ttcgaagctt gagctcggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
     5521 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
     5581 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
     5641 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
     5701 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct
     5761 gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact
     5821 agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag
     5881 gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat
     5941 gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt
     6001 ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc
     6061 tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac
     6121 tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg
     6181 ttcaattttt actactttat atttatataa ttcacttctc caattgtccc tcatatctcc
     6241 tccttatggt gttttactaa acttttccat gttctaatcc tcatcctgtc tacttgccac
     6301 acaatcatca cctgccatct gttttccata atccctaatg atctttgctt ttcttcttgg
     6361 cactactttt atgtcactat tatcttgtat tactactgcc ccttcacctt tccagaggag
     6421 ctttgctggt cctttccaaa gtggatttct gctgtccctg taataaaccc gaaaattttg
     6481 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
     6541 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa
     6601 tactgccatt tgtactgctg tcttaagatg ttcagcctga tctcttacct gtcctataat
     6661 tttctttaat tctttattca tagattctac tactccttga ctttggggat tgtagggaat
     6721 ttccaggtct gaagatcctg ctgcttgctg tgcttttttc ttacttttgt tttgctcttc
     6781 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     6841 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     6901 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc cagtattaac
     6961 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     7021 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     7081 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     7141 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     7201 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     7261 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     7321 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     7381 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     7441 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag
     7501 gccacgcctc ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     7561 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     7621 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     7681 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     7741 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     7801 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     7861 tactattaat aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt
     7921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9301 cagtggcgat aagtcgtgtc ttaccgg
//
