LOCUS       sequence-406645-       18146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for the production of recombinant baculovirus using MultiMate
            assembly. All-in-one vector for PE3 HEK3 prime editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18146)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 18146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: MultiMate-PE3 HEK3
FEATURES             Location/Qualifiers
     source          1..18146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    127..147
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        165..413
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        434..509
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    579..599
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        608..699
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1697..1831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1875..1966
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             join(2038..2085,2086..3573)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3766..3901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4010..4030
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        4042..4421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4422..4625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4761..5030
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     CDS             5081..5779
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5789..6377
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5842..5862)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6260..6271)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6393..6417)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        6466..6845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6846..7049
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7091..7110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7091..7109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7159..7179
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7180..9693,9694..9696,9697..11280)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7180..11280
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13453..13473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13482..13499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    13531..13665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  13786..13951
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      14141..14729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14630..14649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  14864..15087
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(15154..15687)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(15876..15881,15882..15898,15899..15904))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(15975..16008)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(16077..16152)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(16173..16421)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        16581..16683
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             16684..17343
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(16750..16769)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      17738..18094
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa catcaacaag tttgtacaaa aaagcaggct gtttaaacga tatccctagg
      121 cccgggcaac tttgtataat aaagttggtt taaacgatat caccgagggc ctatttccca
      181 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
      241 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
      301 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
      361 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accggcccag
      421 actgagcacg tgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      481 aacttgaaaa agtgggaccg agtcggtcct ctgccatcaa agcgtgctca gtctgttttt
      541 ttaagcttgg gccgctcgag gtatccctag gcccgggcca actttgtata gaaaagttgg
      601 tttaaacatc atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac
      661 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
      721 cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa ttcaaaggga agtgccattc
      781 cgcctgacct atggcttcac tggttaaaaa agacatgtgc atcaaaatga cgatggaagg
      841 aacagtaaac ggtcaccatt tcaagtgtgt aggagaaggc gaaggcaaac catttgaagg
      901 gacccaggtg gaaaagatac gcatcactga aggtgggccc ttaccatttg cgtatgatat
      961 tttggcccct tgttgcatgt atggcagtaa aaccttcatt aagcatgtgt cgggtattcc
     1021 ggattacttt aaggagtctt ttcctgaggg ctttacctgg gaaagaacac aaatcttcga
     1081 ggatggcggc tatctcacca tacaccagga cacgagcctt cagggtaata attttatttt
     1141 caaagttaat gtcatcggtg ccaacttccc tgcaaacggt cccgtgatgc agaaaaaaac
     1201 agctggatgg gaaccgtgcg ttgagatgct ttatccgcgg gacggcgtcc tgtgtggtca
     1261 gagcctgatg gccctgaaat gcactgatgg caatcatctg acgtcccacc tgcgcactac
     1321 ctatcgttct cgcaagccat ccaatgcagt taacatgccg gaatttcatt ttggggatca
     1381 tcgcattgag attttgaaag ctgaacaagg taaattttat gaacaatacg agtcagcggt
     1441 ggcccgttac tgtgaggcgg caccgagtaa attagggcat cactaaaggc taggtggagg
     1501 ctcagtgcct acgtcgacga gctcacttgt cgcggccgct ttcgaatcta gagcctgcag
     1561 tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     1621 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1681 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1801 actcatcaat gtatcttatc atgtctggat caaacactag tatcgattcg cgacctactc
     1861 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     1921 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     1981 ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggccatg
     2041 aagtgccttt tgtacttagc ctttttattc attggggtga attgcaagtt caccatagtt
     2101 tttccacaca accaaaaagg aaactggaaa aatgttcctt ctaattacca ttattgcccg
     2161 tcaagctcag atttaaattg gcataatgac ttaataggca cagccttaca agtcaaaatg
     2221 cccaagagtc acaaggctat tcaagcagac ggttggatgt gtcatgcttc caaatgggtc
     2281 actacttgtg atttccgctg gtatggaccg aagtatataa cacattccat ccgatccttc
     2341 actccatctg tagaacaatg caaggaaagc attgaacaaa cgaaacaagg aacttggctg
     2401 aatccaggct tccctcctca aagttgtgga tatgcaactg tgacggatgc cgaagcagtg
     2461 attgtccagg tgactcctca ccatgtgctg gttgatgaat acacaggaga atgggttgat
     2521 tcacagttca tcaacggaaa atgcagcaat tacatatgcc ccactgtcca taactctaca
     2581 acctggcatt ctgactataa ggtcaaaggg ctatgtgatt ctaacctcat ttccatggac
     2641 atcaccttct tctcagagga cggagagcta tcatccctgg gaaaggaggg cacagggttc
     2701 agaagtaact actttgctta tgaaactgga ggcaaggcct gcaaaatgca atactgcaag
     2761 cattggggag tcagactccc atcaggtgtc tggttcgaga tggctgataa ggatctcttt
     2821 gctgcagcca gattccctga atgcccagaa gggtcaagta tctctgctcc atctcagacc
     2881 tcagtggatg taagtctaat tcaggacgtt gagaggatct tggattattc cctctgccaa
     2941 gaaacctgga gcaaaatcag agcgggtctt ccaatctctc cagtggatct cagctatctt
     3001 gctcctaaaa acccaggaac cggtcctgct ttcaccataa tcaatggtac cctaaaatac
     3061 tttgagacca gatacatcag agtcgatatt gctgctccaa tcctctcaag aatggtcgga
     3121 atgatcagtg gaactaccac agaaagggaa ctgtgggatg actgggcacc atatgaagac
     3181 gtggaaattg gacccaatgg agttctgagg accagttcag gatataagtt tcctttatac
     3241 atgattggac atggtatgtt ggactccgat cttcatctta gctcaaaggc tcaggtgttc
     3301 gaacatcctc acattcaaga cgctgcttcg caacttcctg atgatgagag tttatttttt
     3361 ggtgatactg ggctatccaa aaatccaatc gagcttgtag aaggttggtt cagtagttgg
     3421 aaaagctcta ttgcctcttt tttctttatc atagggttaa tcattggact attcttggtt
     3481 ctccgagttg gtatccatct ttgcattaaa ttaaagcaca ccaagaaaag acagatttat
     3541 acagacatag agatgaaccg acttggaaag tgataaccta cgtcgacgag ctcacttgtc
     3601 gcggccgctt tcgaatctag agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg
     3661 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3721 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     3901 ctgatcactg cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt
     3961 tggctgctgc caccgctgag caataactat cataacccct aggcccgggc aactttgtat
     4021 acaaaagttg gtttaaacga tgacattgat tattgactag ttattaatag taatcaatta
     4081 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     4141 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     4201 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     4261 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     4321 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     4381 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     4441 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     4501 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     4561 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     4621 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     4681 atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga
     4741 ttccccgtgc caagagtgac gtaagtaccg cctatagact ctataggcac acccctttgg
     4801 ctcttatgca tgctatactg tttttggctt ggggcctata cacccccgct tccttatgct
     4861 ataggtgatg gtatagctta gcctataggt gtgggttatt gaccattatt gaccactcca
     4921 acggtggagg gcagtgtagt ctgagcagta ctcgttgctg ccgcgcgcgc caccagacat
     4981 aatagctgac agactaacag actgttcctt tccatgggtc ttttctgcag tcaccgtcgt
     5041 cgacggtatc gataagcttg atatcgaatt cgccgccgcc atgagcgagc tgattaagga
     5101 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     5161 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     5221 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     5281 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     5341 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     5401 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     5461 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     5521 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     5581 gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct
     5641 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     5701 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     5761 actggggcac aagcttaatg gatcctagaa tcaacctctg gattacaaaa tttgtgaaag
     5821 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5881 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5941 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6001 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6061 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6121 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6181 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6241 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6301 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6361 ttgggccgcc tccccgcatc cctaggcccg ggagacagct ttcttgtaca aagtggttga
     6421 taagcttaaa cactagtatc gatcgcgatg tacgggccag atatagacat tgattattga
     6481 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     6541 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     6601 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     6661 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     6721 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6781 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     6841 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6901 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6961 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     7021 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagagat
     7081 ccgcggccgc taatacgact cactataggg agagccgcca ccatgaaacg gacagccgac
     7141 ggaagcgagt tcgagtcacc aaagaagaag cggaaagtcg acaagaagta cagcatcggc
     7201 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     7261 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     7321 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     7381 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     7441 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     7501 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     7561 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     7621 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     7681 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     7741 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     7801 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     7861 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     7921 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     7981 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     8041 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     8101 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     8161 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     8221 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     8281 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     8341 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     8401 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     8461 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     8521 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     8581 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     8641 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     8701 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     8761 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     8821 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     8881 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     8941 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     9001 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     9061 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     9121 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     9181 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     9241 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     9301 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     9361 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     9421 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     9481 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     9541 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     9601 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     9661 gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag ctttctgaag
     9721 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     9781 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     9841 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     9901 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     9961 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
    10021 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
    10081 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
    10141 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
    10201 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
    10261 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
    10321 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
    10381 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
    10441 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
    10501 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
    10561 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
    10621 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
    10681 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
    10741 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
    10801 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
    10861 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
    10921 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
    10981 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
    11041 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
    11101 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
    11161 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
    11221 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac
    11281 tctggaggat ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca
    11341 acaccagaga gcagtggcgg cagcagcggc ggcagcagca ccctaaatat agaagatgag
    11401 tatcggctac atgagacctc aaaagagcca gatgtttctc tagggtccac atggctgtct
    11461 gattttcctc aggcctgggc ggaaaccggg ggcatgggac tggcagttcg ccaagctcct
    11521 ctgatcatac ctctgaaagc aacctctacc cccgtgtcca taaaacaata ccccatgtca
    11581 caagaagcca gactggggat caagccccac atacagagac tgttggacca gggaatactg
    11641 gtaccctgcc agtccccctg gaacacgccc ctgctacccg ttaagaaacc agggactaat
    11701 gattataggc ctgtccagga tctgagagaa gtcaacaagc gggtggaaga catccacccc
    11761 accgtgccca acccttacaa cctcttgagc gggctcccac cgtcccacca gtggtacact
    11821 gtgcttgatt taaaggatgc ctttttctgc ctgagactcc accccaccag tcagcctctc
    11881 ttcgcctttg agtggagaga tccagagatg ggaatctcag gacaattgac ctggaccaga
    11941 ctcccacagg gtttcaaaaa cagtcccacc ctgtttaatg aggcactgca cagagaccta
    12001 gcagacttcc ggatccagca cccagacttg atcctgctac agtacgtgga tgacttactg
    12061 ctggccgcca cttctgagct agactgccaa caaggtactc gggccctgtt acaaacccta
    12121 gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa tttgccagaa acaggtcaag
    12181 tatctggggt atcttctaaa agagggtcag agatggctga ctgaggccag aaaagagact
    12241 gtgatggggc agcctactcc gaagacccct cgacaactaa gggagttcct agggaaggca
    12301 ggcttctgtc gcctcttcat ccctgggttt gcagaaatgg cagcccccct gtaccctctc
    12361 accaaaccgg ggactctgtt taattggggc ccagaccaac aaaaggccta tcaagaaatc
    12421 aagcaagctc ttctaactgc cccagccctg gggttgccag atttgactaa gccctttgaa
    12481 ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc taacgcaaaa actgggacct
    12541 tggcgtcggc cggtggccta cctgtccaaa aagctagacc cagtagcagc tgggtggccc
    12601 ccttgcctac ggatggtagc agccattgcc gtactgacaa aggatgcagg caagctaacc
    12661 atgggacagc cactagtcat tctggccccc catgcagtag aggcactagt caaacaaccc
    12721 cccgaccgct ggctttccaa cgcccggatg actcactatc aggccttgct tttggacacg
    12781 gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg ctacgctgct cccactgcct
    12841 gaggaagggc tgcaacacaa ctgccttgat atcctggccg aagcccacgg aacccgaccc
    12901 gacctaacgg accagccgct cccagacgcc gaccacacct ggtacacgga tggaagcagt
    12961 ctcttacaag agggacagcg taaggcggga gctgcggtga ccaccgagac cgaggtaatc
    13021 tgggctaaag ccctgccagc cgggacatcc gctcagcggg ctgaactgat agcactcacc
    13081 caggccctaa agatggcaga aggtaagaag ctaaatgttt atactgatag ccgttatgct
    13141 tttgctactg cccatatcca tggagaaata tacagaaggc gtgggtggct cacatcagaa
    13201 ggcaaagaga tcaaaaataa agacgagatc ttggccctac taaaagccct ctttctgccc
    13261 aaaagactta gcataatcca ttgtccagga catcaaaagg gacacagcgc cgaggctaga
    13321 ggcaaccgga tggctgacca agcggcccga aaggcagcca tcacagagac tccagacacc
    13381 tctaccctcc tcatagaaaa ttcatcaccc tctggcggct caaaaagaac cgccgacggc
    13441 agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg tcatcatcac catcaccatt
    13501 gagtttaaac tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    13561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    13621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
    13681 cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
    13741 tgagcaataa ctatcataac ccctagggta tacccatcta attggaacca gataagtgaa
    13801 atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc
    13861 agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc
    13921 tcccagttcc caactatttt gtccgcccac aaccggttga cttgggtcaa ctgtcagacc
    13981 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    14041 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    14101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    14161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    14221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    14281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    14341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    14401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    14461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    14521 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    14581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    14641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    14701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    14761 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattgacttg
    14821 ggtcgctctt cctgtggatg cgcagatgcc ctgcgtaagc gggtgtgggc ggacaataaa
    14881 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
    14941 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
    15001 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
    15061 ggccaagggc atgtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc
    15121 ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc
    15181 aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc
    15241 accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag
    15301 gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc
    15361 atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc
    15421 gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa
    15481 gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc
    15541 acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca
    15601 tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg
    15661 cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
    15721 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat
    15781 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
    15841 accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg
    15901 tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca
    15961 gcagcgaagt cgccataact tcgtatagca tacattatac gaagttatct gccaggcaca
    16021 tgggttttac tagtatcgat tcgcgatgta cgggcccggg cctagggata aaaaaagcac
    16081 cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc
    16141 tagctctaaa acgcaccggg atactggttg acggtgtttc gtcctttcca caagatatat
    16201 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
    16261 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
    16321 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
    16381 cgtatatgca aatatgaagg aatcatggga aataggccct cggtgatatc ggatccggct
    16441 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca
    16501 taacccctag gtgccatttc attacctctt tctccgcacc cgacatagat ctgggccaac
    16561 ttttggcgaa aatgagacgt tgatcggcac gtaagagggt ccaactttca ccataatgaa
    16621 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
    16681 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
    16741 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
    16801 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
    16861 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
    16921 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
    16981 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
    17041 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
    17101 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
    17161 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
    17221 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
    17281 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
    17341 taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt
    17401 gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag
    17461 ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg
    17521 gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg
    17581 tggaggtaat aattgacgat atgatcattt attctgcctc ccagctgaca ttcatccggg
    17641 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
    17701 gccctgagtg cttgcggcag cgtgaagcta attcccatgt cagccgttaa gtgttcctgt
    17761 gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca tccctggctt
    17821 gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct tttttttctt
    17881 ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca
    17941 aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt
    18001 acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag
    18061 cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac tgctgatcaa
    18121 cagatcctct acgcggccgc ggtacc
//
