LOCUS       sequence-406647-        5296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue 
            chromoprotein and VSV-G glycoprotein under the control of viral polH
            promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: pMMK ENTR 3 polH aeBlue polH VSV-G
FEATURES             Location/Qualifiers
     source          1..5296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    605..700
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        709..800
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1798..1932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1976..2067
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             join(2139..2186,2187..3674)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3867..4002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4111..4234
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4330..4349)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4341..5150
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4408..4427)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag
      661 caatgctttc ttataatgcc aactttgtat agaaaagttg gtttaaacat catggagata
      721 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      781 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
      841 cccggtccga agcgcgcgga attcaaaggg aagtgccatt ccgcctgacc tatggcttca
      901 ctggttaaaa aagacatgtg catcaaaatg acgatggaag gaacagtaaa cggtcaccat
      961 ttcaagtgtg taggagaagg cgaaggcaaa ccatttgaag ggacccaggt ggaaaagata
     1021 cgcatcactg aaggtgggcc cttaccattt gcgtatgata ttttggcccc ttgttgcatg
     1081 tatggcagta aaaccttcat taagcatgtg tcgggtattc cggattactt taaggagtct
     1141 tttcctgagg gctttacctg ggaaagaaca caaatcttcg aggatggcgg ctatctcacc
     1201 atacaccagg acacgagcct tcagggtaat aattttattt tcaaagttaa tgtcatcggt
     1261 gccaacttcc ctgcaaacgg tcccgtgatg cagaaaaaaa cagctggatg ggaaccgtgc
     1321 gttgagatgc tttatccgcg ggacggcgtc ctgtgtggtc agagcctgat ggccctgaaa
     1381 tgcactgatg gcaatcatct gacgtcccac ctgcgcacta cctatcgttc tcgcaagcca
     1441 tccaatgcag ttaacatgcc ggaatttcat tttggggatc atcgcattga gattttgaaa
     1501 gctgaacaag gtaaatttta tgaacaatac gagtcagcgg tggcccgtta ctgtgaggcg
     1561 gcaccgagta aattagggca tcactaaagg ctaggtggag gctcagtgcc tacgtcgacg
     1621 agctcacttg tcgcggccgc tttcgaatct agagcctgca gtctcgacaa gcttgtcgag
     1681 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1741 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1921 catgtctgga tcaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
     1981 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     2041 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
     2101 cgcggatccc ggtccgaagc gcgcggaatt caaaggccat gaagtgcctt ttgtacttag
     2161 cctttttatt cattggggtg aattgcaagt tcaccatagt ttttccacac aaccaaaaag
     2221 gaaactggaa aaatgttcct tctaattacc attattgccc gtcaagctca gatttaaatt
     2281 ggcataatga cttaataggc acagccttac aagtcaaaat gcccaagagt cacaaggcta
     2341 ttcaagcaga cggttggatg tgtcatgctt ccaaatgggt cactacttgt gatttccgct
     2401 ggtatggacc gaagtatata acacattcca tccgatcctt cactccatct gtagaacaat
     2461 gcaaggaaag cattgaacaa acgaaacaag gaacttggct gaatccaggc ttccctcctc
     2521 aaagttgtgg atatgcaact gtgacggatg ccgaagcagt gattgtccag gtgactcctc
     2581 accatgtgct ggttgatgaa tacacaggag aatgggttga ttcacagttc atcaacggaa
     2641 aatgcagcaa ttacatatgc cccactgtcc ataactctac aacctggcat tctgactata
     2701 aggtcaaagg gctatgtgat tctaacctca tttccatgga catcaccttc ttctcagagg
     2761 acggagagct atcatccctg ggaaaggagg gcacagggtt cagaagtaac tactttgctt
     2821 atgaaactgg aggcaaggcc tgcaaaatgc aatactgcaa gcattgggga gtcagactcc
     2881 catcaggtgt ctggttcgag atggctgata aggatctctt tgctgcagcc agattccctg
     2941 aatgcccaga agggtcaagt atctctgctc catctcagac ctcagtggat gtaagtctaa
     3001 ttcaggacgt tgagaggatc ttggattatt ccctctgcca agaaacctgg agcaaaatca
     3061 gagcgggtct tccaatctct ccagtggatc tcagctatct tgctcctaaa aacccaggaa
     3121 ccggtcctgc tttcaccata atcaatggta ccctaaaata ctttgagacc agatacatca
     3181 gagtcgatat tgctgctcca atcctctcaa gaatggtcgg aatgatcagt ggaactacca
     3241 cagaaaggga actgtgggat gactgggcac catatgaaga cgtggaaatt ggacccaatg
     3301 gagttctgag gaccagttca ggatataagt ttcctttata catgattgga catggtatgt
     3361 tggactccga tcttcatctt agctcaaagg ctcaggtgtt cgaacatcct cacattcaag
     3421 acgctgcttc gcaacttcct gatgatgaga gtttattttt tggtgatact gggctatcca
     3481 aaaatccaat cgagcttgta gaaggttggt tcagtagttg gaaaagctct attgcctctt
     3541 ttttctttat catagggtta atcattggac tattcttggt tctccgagtt ggtatccatc
     3601 tttgcattaa attaaagcac accaagaaaa gacagattta tacagacata gagatgaacc
     3661 gacttggaaa gtgataacct acgtcgacga gctcacttgt cgcggccgct ttcgaatcta
     3721 gagcctgcag tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca
     3781 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3901 aaagcaatag catcacaaat ttcacaaata aagcattttt tttcactgca ttctagttgt
     3961 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
     4021 agaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     4081 gcaataacta tcataacccc taggcccggg caactttgta tacaaaagtt gaacgagaaa
     4141 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     4201 aatactgtaa aacacaacat atccagtcac tatgctggcc cgtgtctcaa aatctctgat
     4261 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     4321 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     4381 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     4441 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     4501 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     4561 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     4621 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     4681 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     4741 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     4801 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     4861 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     4921 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     4981 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     5041 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     5101 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     5161 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     5221 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     5281 agatcaaagg atcttc
//
