LOCUS       sequence-406650-       15974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for the production of recombinant baculovirus using MultiMate
            assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor
            in MultiBac system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 15974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: MultiMate-PE2 HEK3
FEATURES             Location/Qualifiers
     source          1..15974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    50..184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  305..470
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      660..1248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1149..1168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  1383..1606
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1673..2206)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2395..2400,2401..2417,2418..2423))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2494..2527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2569..2593
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2620..2640
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2658..2906
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2658..2678
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2829..2848
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2927..3002
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    3072..3092
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3101..3192
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4190..4324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4368..4459
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             join(4531..4578,4579..6066)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    6259..6394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6503..6523
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        6535..6914
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6915..7118
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          7254..7523
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     CDS             7574..8272
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8282..8870
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8335..8355)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8753..8764)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8886..8910)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        8959..9338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9339..9542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9584..9603
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9584..9602
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9652..9672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9673..12186,12187..12189,12190..13773)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9673..13773
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             15946..15966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catcatcacc atcaccattg agtttaaact gaatgcaatt gttgttgtta acttgtttat
       61 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      121 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      181 gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg
      241 agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa
      301 ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
      361 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
      421 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca accggttgac
      481 ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      541 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      601 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1321 ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg
     1381 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     1441 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     1501 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     1561 tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca
     1621 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     1681 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     1741 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     1801 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     1861 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     1921 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     1981 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     2041 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     2101 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     2161 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     2221 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     2281 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     2341 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     2401 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     2461 cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat acattatacg
     2521 aagttatctg taactataac ggtcctaagg tagcgagttt aaacatcaac aagtttgtac
     2581 aaaaaagcag gctgtttaaa cgatatccct aggcccgggc aactttgtat aataaagttg
     2641 gtttaaacga tatcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     2701 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     2761 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     2821 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     2881 atatcttgtg gaaaggacga aacaccggcc cagactgagc acgtgagttt tagagctaga
     2941 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggga ccgagtcggt
     3001 cctctgccat caaagcgtgc tcagtctgtt tttttaagct tgggccgctc gaggtatccc
     3061 taggcccggg ccaactttgt atagaaaagt tggtttaaac atcatggaga taattaaaat
     3121 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     3181 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccggtcc
     3241 gaagcgcgcg gaattcaaag ggaagtgcca ttccgcctga cctatggctt cactggttaa
     3301 aaaagacatg tgcatcaaaa tgacgatgga aggaacagta aacggtcacc atttcaagtg
     3361 tgtaggagaa ggcgaaggca aaccatttga agggacccag gtggaaaaga tacgcatcac
     3421 tgaaggtggg cccttaccat ttgcgtatga tattttggcc ccttgttgca tgtatggcag
     3481 taaaaccttc attaagcatg tgtcgggtat tccggattac tttaaggagt cttttcctga
     3541 gggctttacc tgggaaagaa cacaaatctt cgaggatggc ggctatctca ccatacacca
     3601 ggacacgagc cttcagggta ataattttat tttcaaagtt aatgtcatcg gtgccaactt
     3661 ccctgcaaac ggtcccgtga tgcagaaaaa aacagctgga tgggaaccgt gcgttgagat
     3721 gctttatccg cgggacggcg tcctgtgtgg tcagagcctg atggccctga aatgcactga
     3781 tggcaatcat ctgacgtccc acctgcgcac tacctatcgt tctcgcaagc catccaatgc
     3841 agttaacatg ccggaatttc attttgggga tcatcgcatt gagattttga aagctgaaca
     3901 aggtaaattt tatgaacaat acgagtcagc ggtggcccgt tactgtgagg cggcaccgag
     3961 taaattaggg catcactaaa ggctaggtgg aggctcagtg cctacgtcga cgagctcact
     4021 tgtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg agaagtacta
     4081 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     4141 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4201 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4261 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4321 gatcaaacac tagtatcgat tcgcgaccta ctccggaata ttaatagatc atggagataa
     4381 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt
     4441 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc
     4501 ccggtccgaa gcgcgcggaa ttcaaaggcc atgaagtgcc ttttgtactt agccttttta
     4561 ttcattgggg tgaattgcaa gttcaccata gtttttccac acaaccaaaa aggaaactgg
     4621 aaaaatgttc cttctaatta ccattattgc ccgtcaagct cagatttaaa ttggcataat
     4681 gacttaatag gcacagcctt acaagtcaaa atgcccaaga gtcacaaggc tattcaagca
     4741 gacggttgga tgtgtcatgc ttccaaatgg gtcactactt gtgatttccg ctggtatgga
     4801 ccgaagtata taacacattc catccgatcc ttcactccat ctgtagaaca atgcaaggaa
     4861 agcattgaac aaacgaaaca aggaacttgg ctgaatccag gcttccctcc tcaaagttgt
     4921 ggatatgcaa ctgtgacgga tgccgaagca gtgattgtcc aggtgactcc tcaccatgtg
     4981 ctggttgatg aatacacagg agaatgggtt gattcacagt tcatcaacgg aaaatgcagc
     5041 aattacatat gccccactgt ccataactct acaacctggc attctgacta taaggtcaaa
     5101 gggctatgtg attctaacct catttccatg gacatcacct tcttctcaga ggacggagag
     5161 ctatcatccc tgggaaagga gggcacaggg ttcagaagta actactttgc ttatgaaact
     5221 ggaggcaagg cctgcaaaat gcaatactgc aagcattggg gagtcagact cccatcaggt
     5281 gtctggttcg agatggctga taaggatctc tttgctgcag ccagattccc tgaatgccca
     5341 gaagggtcaa gtatctctgc tccatctcag acctcagtgg atgtaagtct aattcaggac
     5401 gttgagagga tcttggatta ttccctctgc caagaaacct ggagcaaaat cagagcgggt
     5461 cttccaatct ctccagtgga tctcagctat cttgctccta aaaacccagg aaccggtcct
     5521 gctttcacca taatcaatgg taccctaaaa tactttgaga ccagatacat cagagtcgat
     5581 attgctgctc caatcctctc aagaatggtc ggaatgatca gtggaactac cacagaaagg
     5641 gaactgtggg atgactgggc accatatgaa gacgtggaaa ttggacccaa tggagttctg
     5701 aggaccagtt caggatataa gtttccttta tacatgattg gacatggtat gttggactcc
     5761 gatcttcatc ttagctcaaa ggctcaggtg ttcgaacatc ctcacattca agacgctgct
     5821 tcgcaacttc ctgatgatga gagtttattt tttggtgata ctgggctatc caaaaatcca
     5881 atcgagcttg tagaaggttg gttcagtagt tggaaaagct ctattgcctc ttttttcttt
     5941 atcatagggt taatcattgg actattcttg gttctccgag ttggtatcca tctttgcatt
     6001 aaattaaagc acaccaagaa aagacagatt tatacagaca tagagatgaa ccgacttgga
     6061 aagtgataac ctacgtcgac gagctcactt gtcgcggccg ctttcgaatc tagagcctgc
     6121 agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
     6181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     6241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6301 agcatcacaa atttcacaaa taaagcattt tttttcactg cattctagtt gtggtttgtc
     6361 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc
     6421 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     6481 tatcataacc cctaggcccg ggcaactttg tatacaaaag ttggtttaaa cgatgacatt
     6541 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     6601 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     6661 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     6721 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     6781 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     6841 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     6901 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     6961 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     7021 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     7081 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     7141 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     7201 tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta
     7261 ccgcctatag actctatagg cacacccctt tggctcttat gcatgctata ctgtttttgg
     7321 cttggggcct atacaccccc gcttccttat gctataggtg atggtatagc ttagcctata
     7381 ggtgtgggtt attgaccatt attgaccact ccaacggtgg agggcagtgt agtctgagca
     7441 gtactcgttg ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc
     7501 ctttccatgg gtcttttctg cagtcaccgt cgtcgacggt atcgataagc ttgatatcga
     7561 attcgccgcc gccatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     7621 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     7681 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     7741 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     7801 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     7861 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     7921 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     7981 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     8041 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     8101 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     8161 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     8221 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta atggatccta
     8281 gaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8341 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8401 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8461 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8521 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8581 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8641 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     8701 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8761 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8821 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atccctaggc
     8881 ccgggagaca gctttcttgt acaaagtggt tgataagctt aaacactagt atcgatcgcg
     8941 atgtacgggc cagatataga cattgattat tgactagtta ttaatagtaa tcaattacgg
     9001 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     9061 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     9121 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     9181 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     9241 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     9301 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     9361 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     9421 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     9481 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     9541 ctggtttagt gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata
     9601 gggagagccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
     9661 aagcggaaag tcgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc
     9721 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     9781 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     9841 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     9901 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     9961 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
    10021 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
    10081 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
    10141 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
    10201 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
    10261 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
    10321 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
    10381 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
    10441 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
    10501 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
    10561 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
    10621 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
    10681 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
    10741 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
    10801 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
    10861 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
    10921 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
    10981 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
    11041 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
    11101 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
    11161 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
    11221 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
    11281 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
    11341 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
    11401 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
    11461 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
    11521 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
    11581 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
    11641 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
    11701 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
    11761 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
    11821 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
    11881 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
    11941 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
    12001 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
    12061 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
    12121 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
    12181 gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
    12241 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
    12301 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
    12361 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
    12421 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
    12481 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
    12541 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
    12601 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
    12661 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
    12721 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
    12781 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
    12841 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
    12901 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
    12961 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
    13021 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
    13081 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
    13141 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
    13201 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
    13261 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
    13321 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
    13381 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
    13441 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
    13501 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
    13561 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
    13621 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
    13681 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
    13741 acacggatcg acctgtctca gctgggaggt gactctggag gatctagcgg aggatcctct
    13801 ggcagcgaga caccaggaac aagcgagtca gcaacaccag agagcagtgg cggcagcagc
    13861 ggcggcagca gcaccctaaa tatagaagat gagtatcggc tacatgagac ctcaaaagag
    13921 ccagatgttt ctctagggtc cacatggctg tctgattttc ctcaggcctg ggcggaaacc
    13981 gggggcatgg gactggcagt tcgccaagct cctctgatca tacctctgaa agcaacctct
    14041 acccccgtgt ccataaaaca ataccccatg tcacaagaag ccagactggg gatcaagccc
    14101 cacatacaga gactgttgga ccagggaata ctggtaccct gccagtcccc ctggaacacg
    14161 cccctgctac ccgttaagaa accagggact aatgattata ggcctgtcca ggatctgaga
    14221 gaagtcaaca agcgggtgga agacatccac cccaccgtgc ccaaccctta caacctcttg
    14281 agcgggctcc caccgtccca ccagtggtac actgtgcttg atttaaagga tgcctttttc
    14341 tgcctgagac tccaccccac cagtcagcct ctcttcgcct ttgagtggag agatccagag
    14401 atgggaatct caggacaatt gacctggacc agactcccac agggtttcaa aaacagtccc
    14461 accctgttta atgaggcact gcacagagac ctagcagact tccggatcca gcacccagac
    14521 ttgatcctgc tacagtacgt ggatgactta ctgctggccg ccacttctga gctagactgc
    14581 caacaaggta ctcgggccct gttacaaacc ctagggaacc tcgggtatcg ggcctcggcc
    14641 aagaaagccc aaatttgcca gaaacaggtc aagtatctgg ggtatcttct aaaagagggt
    14701 cagagatggc tgactgaggc cagaaaagag actgtgatgg ggcagcctac tccgaagacc
    14761 cctcgacaac taagggagtt cctagggaag gcaggcttct gtcgcctctt catccctggg
    14821 tttgcagaaa tggcagcccc cctgtaccct ctcaccaaac cggggactct gtttaattgg
    14881 ggcccagacc aacaaaaggc ctatcaagaa atcaagcaag ctcttctaac tgccccagcc
    14941 ctggggttgc cagatttgac taagcccttt gaactctttg tcgacgagaa gcagggctac
    15001 gccaaaggtg tcctaacgca aaaactggga ccttggcgtc ggccggtggc ctacctgtcc
    15061 aaaaagctag acccagtagc agctgggtgg cccccttgcc tacggatggt agcagccatt
    15121 gccgtactga caaaggatgc aggcaagcta accatgggac agccactagt cattctggcc
    15181 ccccatgcag tagaggcact agtcaaacaa ccccccgacc gctggctttc caacgcccgg
    15241 atgactcact atcaggcctt gcttttggac acggaccggg tccagttcgg accggtggta
    15301 gccctgaacc cggctacgct gctcccactg cctgaggaag ggctgcaaca caactgcctt
    15361 gatatcctgg ccgaagccca cggaacccga cccgacctaa cggaccagcc gctcccagac
    15421 gccgaccaca cctggtacac ggatggaagc agtctcttac aagagggaca gcgtaaggcg
    15481 ggagctgcgg tgaccaccga gaccgaggta atctgggcta aagccctgcc agccgggaca
    15541 tccgctcagc gggctgaact gatagcactc acccaggccc taaagatggc agaaggtaag
    15601 aagctaaatg tttatactga tagccgttat gcttttgcta ctgcccatat ccatggagaa
    15661 atatacagaa ggcgtgggtg gctcacatca gaaggcaaag agatcaaaaa taaagacgag
    15721 atcttggccc tactaaaagc cctctttctg cccaaaagac ttagcataat ccattgtcca
    15781 ggacatcaaa agggacacag cgccgaggct agaggcaacc ggatggctga ccaagcggcc
    15841 cgaaaggcag ccatcacaga gactccagac acctctaccc tcctcataga aaattcatca
    15901 ccctctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg
    15961 aaagtctaac cggt
//
