LOCUS       sequence-406651-       23410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 7 fluorescently labelled proteins in mammalian cells. 
            Can be used to generate recombinant baculovirus particles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23410)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 23410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: MultiMate-Rainbow
FEATURES             Location/Qualifiers
     source          1..23410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        133..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        437..640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(685..687,688..690,691..1401)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(730..751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(991..1010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1338..1359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2905..3026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3045..3065
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        3105..3408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3409..3612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3657..3676
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3657..3675
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3737..4444
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(5631..5648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5637..5861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5881..5901
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        5940..6243
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6244..6447
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6476..6550
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             6566..7273
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6708..6726)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    7389..7510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7529..7549
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        7561..7940
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7941..8144
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8227..8236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8233..8934
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     polyA_signal    9012..9146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9267..9855
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9738..9749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9871..9895)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        9944..10323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10324..10527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             10585..10962
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      10975..10984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    12182..12316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  12437..12602
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      12792..13380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13281..13300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  13515..13738
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(13805..14338)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(14527..14532,14533..14549,14550..14555))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(14626..14659)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    14758..14782
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    14798..15386
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             14904..14915
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15507..15641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(15671..16366)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     promoter        complement(16656..16859)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        16860..17239
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(17251..17271)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(17298..17318)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(17346..17366)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    17385..17506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(19792..19827)
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     CDS             complement(19870..20547)
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             complement(19870..20547)
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     regulatory      20544..20553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(20625..20828)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        20829..21132
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(21165..21189)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    21413..21461
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     21486..21505
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(21597..21706)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             complement(21797..22588)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      23053..23409
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa catcaacaag tttgtacaaa aaagcaggct gtttaaacga tatccccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      541 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      601 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      661 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      781 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      841 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
      901 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      961 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1021 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1081 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1141 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1201 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1261 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     1321 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1381 ggcatggacg agctgtacaa gtccggactc agatctcgag tgcgtgagtg catctccatc
     1441 cacgttggcc aggctggtgt ccagattggc aatgcctgct gggagctcta ctgcctggaa
     1501 cacggcatcc agcccgatgg ccagatgcca agtgacaaga ccattggggg aggagatgac
     1561 tccttcaaca ccttcttcag tgagacgggc gctggcaagc acgtgccccg ggctgtgttt
     1621 gtagacttgg aacccacagt cattgatgaa gttcgcactg gcacctaccg ccagctcttc
     1681 caccctgagc agctcatcac aggcaaggaa gatgctgcca ataactatgc ccgagggcac
     1741 tacaccattg gcaaggagat cattgacctt gtgttggacc gaattcgcaa gctggctgac
     1801 cagtgcaccg gtcttcaggg cttcttggtt ttccacagct ttggtggggg aactggttct
     1861 gggttcacct ccctgctcat ggaacgtctc tcagttgatt atggcaagaa gtccaagctg
     1921 gagttctcca tttacccagc accccaggtt tccacagctg tagttgagcc ctacaactcc
     1981 atcctcacca cccacaccac cctggagcac tctgattgtg ccttcatggt agacaatgag
     2041 gccatctatg acatctgtcg tagaaacctc gatatcgagc gcccaaccta cactaacctt
     2101 aaccgcctta ttagccagat tgtgtcctcc atcactgctt ccctgagatt tgatggagcc
     2161 ctgaatgttg acctgacaga attccagacc aacctggtgc cctacccccg catccacttc
     2221 cctctggcca catatgcccc tgtcatctct gctgagaaag cctaccatga acagctttct
     2281 gtagcagaga tcaccaatgc ttgctttgag ccagccaacc agatggtgaa atgtgaccct
     2341 cgccatggta aatacatggc ttgctgcctg ttgtaccgtg gtgacgtggt tcccaaagat
     2401 gtcaatgctg ccattgccac catcaaaacc aagcgcagca tccagtttgt ggattggtgc
     2461 cccactggct tcaaggttgg catcaactac cagcctccca ctgtggtgcc tggtggagac
     2521 ctggccaagg tacagagagc tgtgtgcatg ctgagcaaca ccacagccat tgctgaggcc
     2581 tgggctcgcc tggaccacaa gtttgacctg atgtatgcca agcgtgcctt tgttcactgg
     2641 tacgtgggtg aggggatgga ggaaggcgag ttttcagagg cccgtgaaga tatggctgcc
     2701 cttgagaagg attatgagga ggttggtgtg gattctgttg aaggagaggg tgaggaagaa
     2761 ggagaggaat actaaggatc caccggatct agataactga tcataatcag ccataccaca
     2821 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2881 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3001 ttgtccaaac tcatcaatgt atcttagata tccctaggcc cgggcaactt tgtataataa
     3061 agttggttta aacgatatct tcatagccca tatatggagt tccgcgttac ataacttacg
     3121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     3181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     3241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     3421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     3481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     3541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     3601 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     3661 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc
     3721 tcaaaggaga tataccatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc
     3781 cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga
     3841 gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga
     3901 gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct acggcaacag
     3961 ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg
     4021 ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga
     4081 catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc
     4141 ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca ccgagaggat
     4201 gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc tggagggcgg
     4261 cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc
     4321 cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa
     4381 ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg acgagctgta
     4441 caagggatca ggatcaggat caatggatga tgatatcgcc gcgctcgtcg tcgacaacgg
     4501 ctccggcatg tgcaaggccg gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc
     4561 catcgtgggg cgccccaggc accagggcgt gatggtgggc atgggtcaga aggattccta
     4621 tgtgggcgac gaggcccaga gcaagagagg catcctcacc ctgaagtacc ccatcgagca
     4681 cggcatcgtc accaactggg acgacatgga gaaaatctgg caccacacct tctacaatga
     4741 gctgcgtgtg gctcccgagg agcaccccgt gctgctgacc gaggcccccc tgaaccccaa
     4801 ggccaaccgc gagaagatga cccagatcat gtttgagacc ttcaacaccc cagccatgta
     4861 cgttgctatc caggctgtgc tatccctgta cgcctctggc cgtaccactg gcatcgtgat
     4921 ggactccggt gacggggtca cccacactgt gcccatctac gaggggtatg ccctccccca
     4981 tgccatcctg cgtctggacc tggctggccg ggacctgact gactacctca tgaagatcct
     5041 caccgagcgc ggctacagct tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa
     5101 ggagaagctg tgctacgtcg ccctggactt cgagcaagag atggccacgg ctgcttccag
     5161 ctcctccctg gagaagagct acgagctgcc tgacggccag gtcatcacca ttggcaatga
     5221 gcggttccgc tgccctgagg cactcttcca gccttccttc ctgggcatgg agtcctgtgg
     5281 catccacgaa actaccttca actccatcat gaagtgtgac gtggacatcc gcaaagacct
     5341 gtacgccaac acagtgctgt ctggcggcac caccatgtac cctggcattg ccgacaggat
     5401 gcagaaggag atcactgccc tggcacccag cacaatgaag atcaagatca ttgctcctcc
     5461 tgagcgcaag tactccgtgt ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca
     5521 gcagatgtgg atcagcaagc aggagtatga cgagtccggc ccctccatcg tccaccgcaa
     5581 atgcttctga ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     5641 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5701 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5761 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5821 agacaatagc aggcatgctg gggatgcggt gggctctatg ggatatccct aggcccgggc
     5881 caactttgta tagaaaagtt ggtttaaacg atatctcata gcccatatat ggagttccgc
     5941 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6001 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     6061 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     6121 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6181 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6241 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     6301 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     6361 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     6421 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcatgtc
     6481 cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc
     6541 gcgcgccaag atccattcgt tgggggtgag caagggcgag gaggataaca tggccatcat
     6601 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     6661 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6721 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     6781 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     6841 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     6901 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     6961 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     7021 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     7081 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     7141 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     7201 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     7261 gctgtacaag taagcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     7321 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     7381 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7441 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7501 atgtatctta gatatcccta ggcccgggca actttgtata caaaagttgg tttaaacgat
     7561 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7621 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7681 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7741 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7801 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7861 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7921 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7981 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     8041 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     8101 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctgaagtg ccattccgcc
     8161 tgacctatgc tgctatccgt gccgttgctg ctcggcctcc tcggcctggc cgtcgccgga
     8221 ccggtcgcca ccatggtgag caagggcgag gagctgatca aggagaacat gagaagcaag
     8281 ctgtacctgg aaggcagcgt gaacggccac cagttcaagt gcacccacga aggggagggc
     8341 aagccctacg agggcaagca gaccaacagg atcaaggtgg tggagggagg ccccctgccg
     8401 ttcgcattcg acatcctggc cacccacttt atgtacggga gcaaggtgtt catcaagtac
     8461 cccgccgacc tccccgatta ttttaagcag tccttccctg agggcttcac atgggagaga
     8521 gtcatggtgt tcgaagacgg gggcgtgctg accgccaccc aggacaccag cctccaggac
     8581 ggcgagctca tctacaacgt caaggtcaga ggggtgaact tcccagccaa cggccccgtg
     8641 atgcagaaga aaacactggg ctgggagccc agcaccgaga ccatgtaccc cgctgacggc
     8701 ggcctggaag gcagatgcga caaggccctg aagctcgtgg gcgggggcca cctgcacgtc
     8761 aacttcaaga ccacatacaa gtccaagaaa cccgtgaaga tgcccggcgt ccactacgtg
     8821 gaccgcagac tggaaagaat caaggaggcc gacaacgaga cctacgtcga gcagtacgag
     8881 cacgctgtgg ccagatactc caacctgggc ggaggcatgg acgagctgta caagggtgga
     8941 ggaggtagat ctggaaagga cgagctgtaa aggctaggtg gaggctcagt gtgaatgcaa
     9001 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9061 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9121 tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct
     9181 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa
     9241 cccctagggt atacccatct aattggaatc aacctctgga ttacaaaatt tgtgaaagat
     9301 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     9361 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     9421 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9481 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9541 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9601 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9661 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9721 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9781 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9841 gggccgcctc cccgcatccc taggcccggg agacagcttt cttgtacaaa gtggttgata
     9901 agcttaaaca ctagtatcga tcgcgatgta cgggccagat atagacattg attattgact
     9961 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    10021 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    10081 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    10141 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    10201 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    10261 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    10321 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
    10381 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
    10441 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
    10501 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
    10561 cttatgtacc gcgggcccgc cgccatgcca gagccagcga agtctgctcc cgccccgaaa
    10621 aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc
    10681 agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac
    10741 accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag
    10801 cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc
    10861 agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg
    10921 tccgagggta ctaaggccat caccaagtac accagcgcta aggatccacc ggtcgccacc
    10981 atggccgaag ggagcgtggc aagacagccc gatctgctga catgcgacga cgaaccaatc
    11041 cacattccag gagctatcca gcctcatgga ctgctgctgg ctctggccgc tgacatgact
    11101 atcgtggcag gctctgataa cctgccagag ctgaccgggc tggctatcgg agcactgatt
    11161 ggccggtccg cagccgacgt gttcgattct gagactcaca atagactgac catcgccctg
    11221 gctgaacctg gggctgcagt gggagcccca attaccgtcg gcttcacaat gcgaaaggac
    11281 gctggcttta tcgggtcctg gcaccgccat gatcagctga tttttctgga gctggaacca
    11341 cctcagagag acgtggcaga gccacaggct ttctttcgga gaacaaacag cgccatcagg
    11401 cgcctgcagg ccgctgagac tctggaatcc gcttgcgcag ccgctgcaca ggaagtgagg
    11461 aagatcaccg ggttcgatcg cgtcatgatc tacagattcg cctcagactt tagcggagaa
    11521 gtgatcgcag aagatcgctg tgccgaggtc gaaagtaaac tgggcctgca ttacccagcc
    11581 tcaaccgtgc ctgcacaggc acgacggctg tatacaatca accccgtgcg gatcattccc
    11641 gacattaatt acagaccagt gcccgtcaca cctgacctga acccagtgac tgggaggccc
    11701 atcgatctga gcttcgccat tctgcgcagc gtgtcccctg tccacctgga gtttatgagg
    11761 aatatcggaa tgcatggcac catgtctatc agtattctga gaggcgaaag gctgtggggg
    11821 ctgatcgtgt gccaccatcg gacaccttac tatgtggacc tggacggcag gcaggcttgt
    11881 gaactggtgg ctcaggtgct ggcttggcag attggcgtga tgaaggagga ataataaggg
    11941 gagacccaag ctggctagtg gatcccggtc cgaagcgcgc ggaattcaaa ggcctacgtc
    12001 gacgagctca cttgtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt
    12061 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
    12121 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    12181 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    12241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    12301 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
    12361 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa cccctagggt
    12421 atacccatct aattggaacc agataagtga aatctagttc caaactattt tgtcattttt
    12481 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
    12541 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
    12601 caaccggttg acttgggtca actgtcagac caagtttact catatatact ttagattgat
    12661 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    12721 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    12781 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    12841 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    12901 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    12961 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    13021 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    13081 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    13141 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    13201 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    13261 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    13321 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    13381 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    13441 ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat gcgcagatgc
    13501 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
    13561 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
    13621 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
    13681 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga ctatattcgc
    13741 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga
    13801 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
    13861 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
    13921 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
    13981 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
    14041 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
    14101 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
    14161 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
    14221 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
    14281 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
    14341 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
    14401 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
    14461 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
    14521 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
    14581 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgccataac ttcgtatagc
    14641 atacattata cgaagttatc tgccaggcac atgggttttt ttcctcctct cctgactact
    14701 cccagtcata gctgtccctc ttctcttatg aagatccctc gacgtttaag cttatcaacc
    14761 actttgtaca agaaagctgt ctcccgggcc tagggatgcg gggaggcggc ccaaagggag
    14821 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
    14881 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
    14941 cgcagaatcc aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac
    15001 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
    15061 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
    15121 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
    15181 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
    15241 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
    15301 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
    15361 caaattttgt aatccagagg ttgattccaa ttagatgggt ataccctagg ggttatgata
    15421 gttattgctc agcggtggca gcagccaact cagcttcctt tcgggctttg ttagcagccg
    15481 gatcttctag gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg
    15541 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
    15601 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
    15661 actaggatcc attaagcttg tgccccagtt tgctagggag gtcgcagtat ctggccactg
    15721 ccacctcgtg ctgctcgacg taggtctcgt tgttggcctc cttgattctt tccagtctgt
    15781 agtccacata gtagacgcca ggcatcttga ggttcttagc gggtttcttg gatctatatg
    15841 tggtcttgat gtttgcgatc agatggctcc cgcccacgag cttcagggcc atgtcgtttc
    15901 tgccttccag gccgccgtca gcggggtaca gcgtctcggt gaaggcctcc cagccgagtg
    15961 ttttcttctg catcacaggg ccgttggatg tgaagttcac ccctctgatc ttgacgttgt
    16021 agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg cccccgtctt
    16081 cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgcttg aagaagtcgg
    16141 ggatgccctg ggtgtggttg atgaaggtct tgctgccgta gaggaagcta gtagccagga
    16201 tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg gtctgggtgc
    16261 cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtgatggttg tccacggtgc
    16321 cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcgctagtc gctactggtg
    16381 ggtctttagc gccgcctgtc ctcagttcgc cgctgctttg tccaatagct gcagcgctat
    16441 ttgaaccgcc ttgcaagggg gtactcacgc ccaccaactg tggaagccgg gagagatctc
    16501 ttcccgcaag ataatacaca agagtcaccc cgagatgcaa tgcacacacc gcaaccagaa
    16561 gacgacatgc cctttgcagg ctggcaccgg gcatggcagc agagcctgac aacaagggtt
    16621 cacgaagcct cataggtcag gcggaatggc acttcagctc tgcttatata gacctcccac
    16681 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
    16741 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
    16801 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
    16861 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
    16921 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
    16981 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
    17041 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
    17101 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
    17161 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
    17221 ctagtcaata atcaatgtca tcgtttaaac caacttttgt atacaaagtt gcccgggcct
    17281 agggatatcg tttaaaccaa cttttctata caaagttggc ccgggcctag ggatatcgtt
    17341 taaaccaact ttattataca aagttgcccg ggcctaggga tatctaagat acattgatga
    17401 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
    17461 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
    17521 cattcattac aggtcagtat caaaccaggc cagctgattg ctatcacctg ggggcagccc
    17581 atccatgagg tcctgggcgt gtcccagatc aggcagccca tcaactggat agtcagcacc
    17641 agggtggtgg ccacccatct catgctccat catagggtcc atccccaagg catcctggcc
    17701 gtatccacca gagtgaaaag aacggtagct gggatcatcc tggcgatatc caagggcttc
    17761 tccctgggca ccaatgtcca gtccaagatc tgcagtctca ttccaagcca ttggctctgt
    17821 cctgaagagg gaactggtca gctcgactga aagccgcttc ttgtaatcct gtggcttgtc
    17881 ctcagacatt cggaatagga cagcagctgc gtatgttgcc acgccttcat tcctggagtg
    17941 gagtaactct gtcaggggag ctgtggctcc ctcagcttca atggcctctg cagcctcctt
    18001 gtcctgagca agttcacaga ggacccctgc agctactctt tggatatttt caatgggaga
    18061 ataaagcaac tgcacaaaca atggaatggt attgagtcct cggattacaa tccggttgtg
    18121 aacgtcccga gcaaggatgt ggagagctcc agtacaccct tctactatct cctccatgcg
    18181 cacgccctcc acaaactgct gctgcgttcc acccatggag gtgcgccgtt gggtgtcctg
    18241 atgtgctcgt acaagcagct gaactagtcg tggaatagca ccctgttccc gcaaaggcgc
    18301 atgatttgct gggcaaaggg caaggtttcg aatcaatcca acagttgcct ttatcagagg
    18361 ccagtgggat ggtgggtgca ggagtttaac cacaacaggc agtccataat gaaggcgaac
    18421 ggcattctgg gccatctcgg cttcctgatg ccggctggtc agatgacgaa gagcacagat
    18481 ggcaggctca gtgatgtctt ccctgtcacc agcacgaagg acggtgcgta caagagcctc
    18541 tatgccaccc acttggcaca ccatcatctt gtttttgtaa ttattgcaag tgaggttaga
    18601 gagaattcca gctgcacagg tgaccacatt tatatcatcg gaacccagaa gctgcactag
    18661 agtcccaagg aggccttcca tcccttcctg cttagtcgct gcatctgaaa ggtttctgag
    18721 agtccaaaga cagttttgaa caagtcgctg acttgggtct gtcagatgaa gccccagtgc
    18781 ctgcatccca ccagcttcta caatggccgg cttgttgcta gagcagacag acagcacctt
    18841 cagcactctg cttgtggtcc acagaagctt ctcataagtg taggtcctca ttatgtttac
    18901 taaggcttgg ggtccaccac tggccagaat gatgagcttg ctctcttgat tgccataagc
    18961 taagatctga aggcagtctg ttgtaatagc caagaatttc acgtttgttt tgttgagcaa
    19021 agcaaccatt ttctgcagtc caccagctag gcgcactgcc attttagctc cttcctgatg
    19081 gagcaggaga ttatgcagtg tcgtgatggc gtagaacagt acagaatcca ctggtgaccc
    19141 aagcattttc accagcgctg ggatgccacc agacttaaag atggccagca agccctcgcg
    19201 gtggtgagaa aggttgtgca gagtcccagc agtacaacga gctgtctcta catcatttgt
    19261 attctgcatg gtgcgtacaa tggcagacac catctgaggg gagcgcatga tggcatgtct
    19321 ggaagcttcc tttttggaaa gctgatggac cataacagca gctttattaa ctaccacctg
    19381 gtcctcatcg tttagcagtt ttgtcagctc aggaattgca cgtgtggcaa gttccgcgtc
    19441 atcctgatag ttaatcaaat tgacaactgc atgtttcaac atctgtgatg gttcagccaa
    19501 gcgctggaca ttagtgggat gagcagcgtc aaactgcgtg gatgggatct gcatgccctc
    19561 atctagcgtc tcagggaaca tggcagctcg gaccctctga gccctagtca ttgcatactg
    19621 cccgtcaata tcagctactt gctcttgcgt gaaggactgg gaaaagcctt gctcccattc
    19681 ataaaggact tgggaggtgt caacatcttc ttcctcaggg ttgcccttgc cactcaggga
    19741 aggagctgtg gtggtggcac cagaatggat tccagaatcc aagtaagact gctgctgcca
    19801 gtggctgaca gcagcttttc tgtccggctc catggccatg tccaactcca tcaggtcagc
    19861 ttgagtagcc ttggcctggc tgggcagcat ggagtacctg gccacggcgt tctcgtacag
    19921 cttcaccttg ttgtagtcct tgtcgtgctt caggatctcg atcctgtggt cgatcttgtg
    19981 ggcgtcgggc agcctcacct ccttcttggc cttgtaggtg gtcttgaagt cgcacctgta
    20041 gtggccgccg ccctccagca gcagcctcat gttcacgtcg cccttcagca cgccgtcctc
    20101 cacgtacatc ttctcggtgc tgggctccca cttcagggtc ttcttctgca tcacggggcc
    20161 gttggggggg aagttggtgc cgtcgaacct gatgtcgtag aagaagcagt cgcccctcat
    20221 gctgatgttg ctggtggcgg tgcagatgcc ctggtcctcg taggtcatgc tcctctccca
    20281 gtggtagccc tcggggaagg tctgcttgaa gtagtcctgg atgtcggcgg ggtacttggt
    20341 gaaggccctg ttgccgtact ggaacacggt ggtcaggatg tcgtaggcga agggcagggg
    20401 ggcgccctcg gtcacgttca ggtccaggat ctgggtgccc tcgtaggggt tgcccttgcc
    20461 ctcgccctcg atcacgaagt tgtggccgtt cacggtgccc ctcatgcaca gcttgatctt
    20521 catctcgggc ttgatcacgc tcaccatggt ggcgaattcc agcacactgg cggccgttac
    20581 tagtggcccg ggcccgcggt accgtcgatc gactgcagaa ttccagctct gcttatatag
    20641 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
    20701 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
    20761 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
    20821 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
    20881 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
    20941 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
    21001 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
    21061 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
    21121 agttatgtaa cgcggaactc catatatggg gatatcgttt aaacagcctg cttttttgta
    21181 caaacttgtt gatgtttaaa ctcgctacct taggaccgtt atagttacag aaacgtaccc
    21241 gtagtggcta tggcagggct tgccgccccg acgttggctg cgagccctgg gccttcaccc
    21301 gaacttgggg gttggggtgg ggaaaaggaa gaaacgcggg cgtattggtc ccaatggggt
    21361 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
    21421 gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
    21481 cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccggtac cgcatgctat
    21541 gcatcagctg ctagcaccat ggctcgagat cccgggtgat caagtcttcg tcgagtgatt
    21601 gtaaataaaa tgtaatttac agtatagtat tttaattaat atacaaatga tttgataata
    21661 attcttattt aactataata tattgtgttg ggttgaatta aaggtccgta tactagtatc
    21721 gatacctagg tgccatttca ttacctcttt ctccgcaccc gacatagatc cgtcggcttg
    21781 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
    21841 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
    21901 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
    21961 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
    22021 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
    22081 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
    22141 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
    22201 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
    22261 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
    22321 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
    22381 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
    22441 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
    22501 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
    22561 atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct
    22621 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
    22681 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga
    22741 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
    22801 agcgcatacg ctacttaccg gtttattgac taccggaagc agtgtgaccg tgtgcttctc
    22861 aaatgcctga ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat
    22921 catttattct gcctcccagc tgacattcat ccggggtcag caccgtttct gcggactggc
    22981 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
    23041 agctaattcc catgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc
    23101 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa
    23161 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta
    23221 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac
    23281 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg
    23341 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt
    23401 agtacgtacc
//
