LOCUS       sequence-406652-       14625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 5 fluorescently labelled proteins in mammalian cells. 
            Can be used as a CRE-donor (MultiBac system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 14625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: MultiMate 5 colors
FEATURES             Location/Qualifiers
     source          1..14625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      596..605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(602..604,605..607,608..1318)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(647..668)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(908..927)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1255..1276
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2822..2943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2962..2982
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        3022..3325
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3326..3529
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3574..3593
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3574..3592
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3654..4361
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(5548..5565)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5554..5778
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5798..5818
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        5857..6160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6161..6364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6393..6467
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             6483..7190
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6625..6643)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    7306..7427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7446..7466
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        7478..7857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7858..8061
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8144..8153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8150..8851
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     polyA_signal    8929..9063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9184..9772
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9237..9257)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9655..9666)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9788..9812)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        9861..10240
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10241..10444
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             10502..10879
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      10892..10901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    12099..12233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  12354..12519
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      12709..13297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13198..13217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  13432..13655
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(13722..14255)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(14444..14449,14450..14466,14467..14472))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(14543..14576)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(14618..14625,1..17)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtacaaaaaa gcaggctgtt taaacgatat ccccatatat ggagttccgc gttacataac
       61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      541 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
      601 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      661 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      721 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      781 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
      841 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      901 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      961 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1021 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     1081 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1141 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1201 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     1261 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     1321 cggactcaga tctcgagtgc gtgagtgcat ctccatccac gttggccagg ctggtgtcca
     1381 gattggcaat gcctgctggg agctctactg cctggaacac ggcatccagc ccgatggcca
     1441 gatgccaagt gacaagacca ttgggggagg agatgactcc ttcaacacct tcttcagtga
     1501 gacgggcgct ggcaagcacg tgccccgggc tgtgtttgta gacttggaac ccacagtcat
     1561 tgatgaagtt cgcactggca cctaccgcca gctcttccac cctgagcagc tcatcacagg
     1621 caaggaagat gctgccaata actatgcccg agggcactac accattggca aggagatcat
     1681 tgaccttgtg ttggaccgaa ttcgcaagct ggctgaccag tgcaccggtc ttcagggctt
     1741 cttggttttc cacagctttg gtgggggaac tggttctggg ttcacctccc tgctcatgga
     1801 acgtctctca gttgattatg gcaagaagtc caagctggag ttctccattt acccagcacc
     1861 ccaggtttcc acagctgtag ttgagcccta caactccatc ctcaccaccc acaccaccct
     1921 ggagcactct gattgtgcct tcatggtaga caatgaggcc atctatgaca tctgtcgtag
     1981 aaacctcgat atcgagcgcc caacctacac taaccttaac cgccttatta gccagattgt
     2041 gtcctccatc actgcttccc tgagatttga tggagccctg aatgttgacc tgacagaatt
     2101 ccagaccaac ctggtgccct acccccgcat ccacttccct ctggccacat atgcccctgt
     2161 catctctgct gagaaagcct accatgaaca gctttctgta gcagagatca ccaatgcttg
     2221 ctttgagcca gccaaccaga tggtgaaatg tgaccctcgc catggtaaat acatggcttg
     2281 ctgcctgttg taccgtggtg acgtggttcc caaagatgtc aatgctgcca ttgccaccat
     2341 caaaaccaag cgcagcatcc agtttgtgga ttggtgcccc actggcttca aggttggcat
     2401 caactaccag cctcccactg tggtgcctgg tggagacctg gccaaggtac agagagctgt
     2461 gtgcatgctg agcaacacca cagccattgc tgaggcctgg gctcgcctgg accacaagtt
     2521 tgacctgatg tatgccaagc gtgcctttgt tcactggtac gtgggtgagg ggatggagga
     2581 aggcgagttt tcagaggccc gtgaagatat ggctgccctt gagaaggatt atgaggaggt
     2641 tggtgtggat tctgttgaag gagagggtga ggaagaagga gaggaatact aaggatccac
     2701 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     2761 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     2821 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2881 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2941 ttagatatcc ctaggcccgg gcaactttgt ataataaagt tggtttaaac gatatcttca
     3001 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3061 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3121 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3181 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3241 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3301 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3361 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3421 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3481 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     3541 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
     3601 agctggctag cgtttaaact taagcttggt accgagctca aaggagatat accatggtga
     3661 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
     3721 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
     3781 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
     3841 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
     3901 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
     3961 ccttcgagga caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct
     4021 tcatctacga gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga
     4081 agaagaccac cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga
     4141 agggcgacgt caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca
     4201 agaccatcta cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc
     4261 gcatcgagat cctgaaccac gacaaggact acaacaaggt gaccgtttac gagagcgccg
     4321 tggcccgcaa ctccaccgac ggcatggacg agctgtacaa gggatcagga tcaggatcaa
     4381 tggatgatga tatcgccgcg ctcgtcgtcg acaacggctc cggcatgtgc aaggccggct
     4441 tcgcgggcga cgatgccccc cgggccgtct tcccctccat cgtggggcgc cccaggcacc
     4501 agggcgtgat ggtgggcatg ggtcagaagg attcctatgt gggcgacgag gcccagagca
     4561 agagaggcat cctcaccctg aagtacccca tcgagcacgg catcgtcacc aactgggacg
     4621 acatggagaa aatctggcac cacaccttct acaatgagct gcgtgtggct cccgaggagc
     4681 accccgtgct gctgaccgag gcccccctga accccaaggc caaccgcgag aagatgaccc
     4741 agatcatgtt tgagaccttc aacaccccag ccatgtacgt tgctatccag gctgtgctat
     4801 ccctgtacgc ctctggccgt accactggca tcgtgatgga ctccggtgac ggggtcaccc
     4861 acactgtgcc catctacgag gggtatgccc tcccccatgc catcctgcgt ctggacctgg
     4921 ctggccggga cctgactgac tacctcatga agatcctcac cgagcgcggc tacagcttca
     4981 ccaccacggc cgagcgggaa atcgtgcgtg acattaagga gaagctgtgc tacgtcgccc
     5041 tggacttcga gcaagagatg gccacggctg cttccagctc ctccctggag aagagctacg
     5101 agctgcctga cggccaggtc atcaccattg gcaatgagcg gttccgctgc cctgaggcac
     5161 tcttccagcc ttccttcctg ggcatggagt cctgtggcat ccacgaaact accttcaact
     5221 ccatcatgaa gtgtgacgtg gacatccgca aagacctgta cgccaacaca gtgctgtctg
     5281 gcggcaccac catgtaccct ggcattgccg acaggatgca gaaggagatc actgccctgg
     5341 cacccagcac aatgaagatc aagatcattg ctcctcctga gcgcaagtac tccgtgtgga
     5401 tcggcggctc catcctggcc tcgctgtcca ccttccagca gatgtggatc agcaagcagg
     5461 agtatgacga gtccggcccc tccatcgtcc accgcaaatg cttctgaccg ctcgagtcta
     5521 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5581 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5641 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5701 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5761 atgcggtggg ctctatggga tatccctagg cccgggccaa ctttgtatag aaaagttggt
     5821 ttaaacgata tctcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5881 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5941 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     6001 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     6061 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     6121 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     6181 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     6241 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     6301 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     6361 agctggttta gtgaaccgtc agatccgcta gcatgtccgt cctgacgccg ctgctgctgc
     6421 ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaagatc cattcgttgg
     6481 gggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     6541 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     6601 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     6661 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     6721 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     6781 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     6841 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     6901 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     6961 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     7021 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     7081 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     7141 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa gcgactctag
     7201 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     7261 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     7321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttagat atccctaggc
     7441 ccgggcaact ttgtatacaa aagttggttt aaacgatgac attgattatt gactagttat
     7501 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     7561 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7621 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7681 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     7741 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7801 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     7861 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     7921 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7981 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     8041 gaggtctata taagcagagc tgaagtgcca ttccgcctga cctatgctgc tatccgtgcc
     8101 gttgctgctc ggcctcctcg gcctggccgt cgccggaccg gtcgccacca tggtgagcaa
     8161 gggcgaggag ctgatcaagg agaacatgag aagcaagctg tacctggaag gcagcgtgaa
     8221 cggccaccag ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcaagcagac
     8281 caacaggatc aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac
     8341 ccactttatg tacgggagca aggtgttcat caagtacccc gccgacctcc ccgattattt
     8401 taagcagtcc ttccctgagg gcttcacatg ggagagagtc atggtgttcg aagacggggg
     8461 cgtgctgacc gccacccagg acaccagcct ccaggacggc gagctcatct acaacgtcaa
     8521 ggtcagaggg gtgaacttcc cagccaacgg ccccgtgatg cagaagaaaa cactgggctg
     8581 ggagcccagc accgagacca tgtaccccgc tgacggcggc ctggaaggca gatgcgacaa
     8641 ggccctgaag ctcgtgggcg ggggccacct gcacgtcaac ttcaagacca catacaagtc
     8701 caagaaaccc gtgaagatgc ccggcgtcca ctacgtggac cgcagactgg aaagaatcaa
     8761 ggaggccgac aacgagacct acgtcgagca gtacgagcac gctgtggcca gatactccaa
     8821 cctgggcgga ggcatggacg agctgtacaa gggtggagga ggtagatctg gaaaggacga
     8881 gctgtaaagg ctaggtggag gctcagtgtg aatgcaattg ttgttgttaa cttgtttatt
     8941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9061 atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga
     9121 gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata cccatctaat
     9181 tggaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9241 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9301 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9361 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     9421 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     9481 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     9541 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     9601 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     9661 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     9721 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatccctag
     9781 gcccgggaga cagctttctt gtacaaagtg gttgataagc ttaaacacta gtatcgatcg
     9841 cgatgtacgg gccagatata gacattgatt attgactagt tattaatagt aatcaattac
     9901 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9961 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    10021 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    10081 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    10141 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    10201 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    10261 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    10321 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
    10381 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    10441 agctctctgg ctaactagag aacccactgc ttactggctt atgtaccgcg ggcccgccgc
    10501 catgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac
    10561 taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat
    10621 ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat
    10681 gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg
    10741 cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg
    10801 cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac
    10861 caagtacacc agcgctaagg atccaccggt cgccaccatg gccgaaggga gcgtggcaag
    10921 acagcccgat ctgctgacat gcgacgacga accaatccac attccaggag ctatccagcc
    10981 tcatggactg ctgctggctc tggccgctga catgactatc gtggcaggct ctgataacct
    11041 gccagagctg accgggctgg ctatcggagc actgattggc cggtccgcag ccgacgtgtt
    11101 cgattctgag actcacaata gactgaccat cgccctggct gaacctgggg ctgcagtggg
    11161 agccccaatt accgtcggct tcacaatgcg aaaggacgct ggctttatcg ggtcctggca
    11221 ccgccatgat cagctgattt ttctggagct ggaaccacct cagagagacg tggcagagcc
    11281 acaggctttc tttcggagaa caaacagcgc catcaggcgc ctgcaggccg ctgagactct
    11341 ggaatccgct tgcgcagccg ctgcacagga agtgaggaag atcaccgggt tcgatcgcgt
    11401 catgatctac agattcgcct cagactttag cggagaagtg atcgcagaag atcgctgtgc
    11461 cgaggtcgaa agtaaactgg gcctgcatta cccagcctca accgtgcctg cacaggcacg
    11521 acggctgtat acaatcaacc ccgtgcggat cattcccgac attaattaca gaccagtgcc
    11581 cgtcacacct gacctgaacc cagtgactgg gaggcccatc gatctgagct tcgccattct
    11641 gcgcagcgtg tcccctgtcc acctggagtt tatgaggaat atcggaatgc atggcaccat
    11701 gtctatcagt attctgagag gcgaaaggct gtgggggctg atcgtgtgcc accatcggac
    11761 accttactat gtggacctgg acggcaggca ggcttgtgaa ctggtggctc aggtgctggc
    11821 ttggcagatt ggcgtgatga aggaggaata ataaggggag acccaagctg gctagtggat
    11881 cccggtccga agcgcgcgga attcaaaggc ctacgtcgac gagctcactt gtcgcggccg
    11941 ctttcgaatc tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa
    12001 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    12061 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    12121 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12181 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
    12241 tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
    12301 gccaccgctg agcaataact atcataaccc ctagggtata cccatctaat tggaaccaga
    12361 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg
    12421 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt
    12481 tccacccctc ccagttccca actattttgt ccgcccacaa ccggttgact tgggtcaact
    12541 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    12601 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    12661 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    12721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    12781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    12841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    12901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    12961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    13021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    13081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    13141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    13201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    13261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    13321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    13381 ttgacttggg tcgctcttcc tgtggatgcg cagatgccct gcgtaagcgg gtgtgggcgg
    13441 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
    13501 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
    13561 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
    13621 aggggcgtgg ccaagggcat gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
    13681 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
    13741 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
    13801 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
    13861 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
    13921 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
    13981 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
    14041 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
    14101 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
    14161 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
    14221 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
    14281 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
    14341 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
    14401 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
    14461 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
    14521 cttgggcagc agcgaagtcg ccataacttc gtatagcata cattatacga agttatctgt
    14581 aactataacg gtcctaaggt agcgagttta aacatcaaca agttt
//
