LOCUS       sequence-406660-        4575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for translational reporter system in Fusobacterium 
            nucleatum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ponath F, Zhu Y, Cosi V, Vogel J
  TITLE     Expanding the genetic toolkit helps dissect a global stress response
            in the early-branching species Fusobacterium nucleatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 
            10.1073/pnas.2201460119. Epub 2022 Sep 26.
  PUBMED    36161895
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-4"; volume: "119(40):e2201460119. 
            doi"; pages: " 10.1073/pnas.2201460119. Epub 2022 Sep 26"
COMMENT     Sequence Label: pVoPo-02
FEATURES             Location/Qualifiers
     source          1..4575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..54
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             1875..1883
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(3399..3410)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      3989..4023
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      join(4121..4575,1..134)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
       61 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggct aaaaaaaata
      181 aaaatattga aaaaacttag aaaatataat agaattaagt acttagatac agctcgaggt
      241 atctaaagga gaagaagata atatagctat aattaaagaa tttatgagat ttaaagttca
      301 tatggaagga tctgttaatg gacatgaatt tgaaatagaa ggagaaggag aaggaagacc
      361 ttatgaaggt actcaaacag ctaaattaaa agtaactaaa ggtggtccat taccatttgc
      421 atgggatata ttaagtcctc aatttatgta tggatctaaa gcatatgtta aacatcctgc
      481 tgatatacct gattatttaa aattatcatt tccagaaggt tttaaatggg aaagagttat
      541 gaattttgaa gatggtggag tagttactgt aacacaagat agttcattac aagatggaga
      601 ttttatatat aaagttaaat taagaggtac taattttcca tctgatggac cagtaatgca
      661 aaagaaaact atgggatggg aagcatcatc tgaaagaatg tatccagaag atggagcatt
      721 aaaaggtgaa attaaacaaa gattaaaatt aaaagatgga ggtcattatg atgctgaagt
      781 taagactaca tataaagcta agaaaccagt tcaattacca ggtgcatata atgttaatat
      841 taaattagat ataacttctc ataatgaaga ttatactata gttgaacaat atgaaagagc
      901 agaaggaaga cattcaacag gaggtatgga tgaattatat aaataaggat cctataagtt
      961 ttaataaaac tttaaataga aaaaggcttc tctcatgaga agtctttttt atttaaaata
     1021 aatataaaat aaaatagagg ctataaatag cctctatttt atgtgagaaa tccctaaata
     1081 aaaagatgcc agtgtgctgg aattcgccct taggctgcgc tagggacctc tttagctccc
     1141 ttttgctggc gaattcttat ggattttagg gtaacaaaaa acaccgtatt tctacgatgt
     1201 ttttgcttaa atacttgttt ttagttacag acaaacctga agttaactat ttatcaattc
     1261 ctgcaattcg tttacaaaac ggcaaatgtg aaatccgtca catactgcgt gatgaacttg
     1321 aattgccaaa ggaagtataa ttttgttatc ttctttataa tatttcccca tagtaaaaat
     1381 aggaatcaaa taatcatatc ctttctgcaa attcagatta aagccatcga aggttgacca
     1441 cggtatcata gatacattaa aaatgttttc cggagcattt ggctttcctt ccattctatg
     1501 attgtttcca taccgttgcg tatcactttc ataatctgct aaaaatgatt taaagtcaga
     1561 cttacactca gtccaaaggc tggaaaatgt ttcagtatca ttgtgaaata ttgtatagct
     1621 tggtatcatc tcatcatata tccccaattc accatcttga ttgattgccg tcctaaactc
     1681 tgaatggcgg tttacaatca ttgcaatata ataaagcatt gcaggatata gtttcattcc
     1741 cttttccttt atttgtgtga tatccacttt aacggtcatg ctgtaggtac aaggtacact
     1801 tgcaaagtag tggtcaaaat actcttttct gttccaacta tttttatcaa ttttttcaaa
     1861 taccatttaa aattacctcc ttaaaatata ctgctttaat tgtataattt tagctaatta
     1921 ttgtcaagta tttttaaagt tatgtaaatt ttttacttat attttatcga tcgttctaga
     1981 tattcttttt taaacttttc aaatgcttca aattctacat ctgcaacttc tacatctttt
     2041 ccagtatttc caaacttcac acaatttttg taaacatttc ttaatttttc caagtattct
     2101 atgtcttcct tttcttcttt aacatactct tgtaactgtg tcatcaaatt agttttgtct
     2161 tccacattcc ccctttttct tgtaattgcc ttatttaatt cttcaaatct tattgttttc
     2221 attttattgt acctccaatt tttttatata tctatgttaa aaaacatatt gacaattgat
     2281 taaaacaatg atattatggt cggttgagct cattgagctg agtgcagact ttcccccttg
     2341 taaaaagggg aaaaaacgat tgataatgat acaatggtcg tatatcgcta tgttcgcatc
     2401 gtttaactta ctttatcgca tatttaatta tatgccagtg ttagccctgc ccaacaccta
     2461 gagttttagc ttactttcta aattataacc ctaataaagt acccactcag agcacctcca
     2521 agacttttaa aggtagcctc gcaccttgct acaattcata ataccaccat tacggtgcta
     2581 taccgtttcg agcagaacgg ctatacatac gaatttatct tatgtataaa atttgttaca
     2641 aatataatag taagtttttt ttatataaaa aagcagatcg cttttatttt ttttgcaacc
     2701 tgcttcacaa ctcatttttt tactttagta aataaatcct ttacatcttt taaaaaatat
     2761 ggtatattaa gtcaaagcag gcataacata tatgttatgt tttatagtaa aaatggacta
     2821 ttcaactatt taaaaaatgg tagaatacaa ataattttcc tggcagtcaa ttatttttta
     2881 aaaattttta ctattatatt tttaaatttt aaattttaga tagagttatt tggcgataac
     2941 tctttttgtc ttacttctga gtttagcata ttttttttgt tttgtaaatc catttcttta
     3001 tttttcttta caacataaac tttttttact tctctataaa tcttctcacc agttgaaaca
     3061 tcaatttcta acagttctct agcaataaaa tttttaatat agagtaaatc tttttcatct
     3121 gtaagtatat cattcatttc atcaaaatcc aacttttttt tggtttcttt tggttttatt
     3181 aaatttcgtg aacagaaata ggcttttttt tgatttgctt tcttttctaa gtctttcaca
     3241 aaatacttcg ttatatacac ccccacagct tcatttttaa cagtttcttt aatcttattt
     3301 atttttataa atccattcgc ccacaattcc ccaagcttct tattatctat atatgggacc
     3361 gagaacagca ccaaatgata atggattgct cccctctgct ggagttccca agttgcaata
     3421 tatttaagct gttgattttt caagtacctt ttcaatctct taataaataa agtaaattca
     3481 cgatttgccc tttctatatc ctgtatattt tctttaaaag taagagttaa aaaggtactt
     3541 ttatcatcat aattacaatc aattatcctt tttatttcaa atcgcttgtc ctggtaatat
     3601 gctttccttc gttgcatact ttcagctttt tcaatgctac ttaattcttc aaaatctctt
     3661 ctttttcttc ctggtcttct tttaaagtca aaagcagtta aagcatcaat attgaaaatg
     3721 tattcagtta tattttttga ttttatttct ttagtgttat aaaatttcaa ctcaataaat
     3781 tcccccttta ttaatctgca taatcttttt tataatgtcc tttacaacct aatattctta
     3841 atctatcatt ttttaagtca taaattagtc tatgttcatc acttattctt ctgctataaa
     3901 tagccctatt tttaaaatgt tttaatctct caggcttacc agtaccgcgg ccgctccagt
     3961 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     4021 tattggtgag aatccaagca ctagtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4261 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4561 cggtaagcgg caggg
//
