LOCUS       sequence-406662-        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mScarlet-I-expression vector for Fusobacterium 
            nucleatum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ponath F, Zhu Y, Cosi V, Vogel J
  TITLE     Expanding the genetic toolkit helps dissect a global stress response
            in the early-branching species Fusobacterium nucleatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 
            10.1073/pnas.2201460119. Epub 2022 Sep 26.
  PUBMED    36161895
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-4"; volume: "119(40):e2201460119. 
            doi"; pages: " 10.1073/pnas.2201460119. Epub 2022 Sep 26"
COMMENT     Sequence Label: pVoPo-mSc
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      360..394
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      1410..1998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1899..1918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             2769..2777
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4293..4304)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttcaatgcta cttaattctt caaaatctct tctttttctt cctggtcttc ttttaaagtc
       61 aaaagcagtt aaagcatcaa tattgaaaat gtattcagtt atattttttg attttatttc
      121 tttagtgtta taaaatttca actcaataaa ttcccccttt attaatctgc ataatctttt
      181 ttataatgtc ctttacaacc taatattctt aatctatcat tttttaagtc ataaattagt
      241 ctatgttcat cacttattct tctgctataa atagccctat ttttaaaatg ttttaatctc
      301 tcaggcttac cagtaccgcg gccgctccag taatgacctc agaactccat ctggatttgt
      361 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtaagc
      421 ataaaaaaaa gttggataat atactaaaat agtgtataat aaatatgtat attagaaagg
      481 aggaatatat aatggtatca aaaggtgaag cagttataaa agagtttatg agatttaaag
      541 ttcatatgga aggtagtatg aatggacatg aatttgagat tgaaggagaa ggagaaggta
      601 gaccttatga aggaacacaa acagctaaac ttaaagttac taaaggagga ccattaccat
      661 tttcttggga tattctttct cctcaattta tgtatggatc aagagctttt attaaacatc
      721 ctgcagatat acctgattat tataaacagt catttcctga aggatttaaa tgggaaagag
      781 ttatgaactt tgaagatgga ggagcagtta cagtaactca agatacttct ttagaagatg
      841 gaacattgat ttataaggta aaacttagag gaactaattt tccacctgat ggaccagtta
      901 tgcaaaagaa aacaatgggt tgggaagcat ctacagaaag attatatcca gaagatggag
      961 ttttaaaagg tgacataaaa atggctttaa gactaaaaga tggaggaaga tatttagcag
     1021 atttcaaaac tacatataaa gctaaaaaac cagtacaaat gcctggagct tataatgtag
     1081 atagaaagtt agacataact agtcacaatg aagattatac agttgttgaa caatatgaaa
     1141 gaagtgaagg tagacattca actggtggta tggatgaatt atacaaataa ggatcctata
     1201 agttttaata aaactttaaa tagaaaaagg cttctctaat gagaagtctt ttttatttaa
     1261 aataaatata aaataaaata gaggctataa atagcctcta ttttatgtga gaaatcccta
     1321 aataaaaaac tagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2041 tggcgaattc ttatggattt tagggtaaca aaaaacaccg tatttctacg atgtttttgc
     2101 ttaaatactt gtttttagtt acagacaaac ctgaagttaa ctatttatca attcctgcaa
     2161 ttcgtttaca aaacggcaaa tgtgaaatcc gtcacatact gcgtgatgaa cttgaattgc
     2221 caaaggaagt ataattttgt tatcttcttt ataatatttc cccatagtaa aaataggaat
     2281 caaataatca tatcctttct gcaaattcag attaaagcca tcgaaggttg accacggtat
     2341 catagataca ttaaaaatgt tttccggagc atttggcttt ccttccattc tatgattgtt
     2401 tccataccgt tgcgtatcac tttcataatc tgctaaaaat gatttaaagt cagacttaca
     2461 ctcagtccaa aggctggaaa atgtttcagt atcattgtga aatattgtat agcttggtat
     2521 catctcatca tatatcccca attcaccatc ttgattgatt gccgtcctaa actctgaatg
     2581 gcggtttaca atcattgcaa tataataaag cattgcagga tatagtttca ttcccttttc
     2641 ctttatttgt gtgatatcca ctttaacggt catgctgtag gtacaaggta cacttgcaaa
     2701 gtagtggtca aaatactctt ttctgttcca actattttta tcaatttttt caaataccat
     2761 ttaaaattac ctccttaaaa tatactgctt taattgtata attttagcta attattgtca
     2821 agtattttta aagttatgta aattttttac ttatatttta tcgatcgttc tagatattct
     2881 tttttaaact tttcaaatgc ttcaaattct acatctgcaa cttctacatc ttttccagta
     2941 tttccaaact tcacacaatt tttgtaaaca tttcttaatt tttccaagta ttctatgtct
     3001 tccttttctt ctttaacata ctcttgtaac tgtgtcatca aattagtttt gtcttccaca
     3061 ttcccccttt ttcttgtaat tgccttattt aattcttcaa atcttattgt tttcatttta
     3121 ttgtacctcc aattttttta tatatctatg ttaaaaaaca tattgacaat tgattaaaac
     3181 aatgatatta tggtcggttg agctcattga gctgagtgca gactttcccc cttgtaaaaa
     3241 ggggaaaaaa cgattgataa tgatacaatg gtcgtatatc gctatgttcg catcgtttaa
     3301 cttactttat cgcatattta attatatgcc agtgttagcc ctgcccaaca cctagagttt
     3361 tagcttactt tctaaattat aaccctaata aagtacccac tcagagcacc tccaagactt
     3421 ttaaaggtag cctcgcacct tgctacaatt cataatacca acattacggt gctataccgt
     3481 ttcgagcaga acggctatac atacgaattt atcttatgta taaaatttgt tacaaatata
     3541 atagtaagtt ttttttatat aaaaaagcag atcgctttta ttttttttgc aacctgcttc
     3601 acaactcatt tttttacttt agtaaataaa tcctttacat cttttaaaaa atatggtata
     3661 ttaagtcaaa gcaggcataa catatatgtt atgttttata gtaaaaatgg actattcaac
     3721 tatttaaaaa atggtagaat acaaataatt ttcctggcag tcaattattt tttaaaaatt
     3781 tttactatta tatttttaaa ttttaaattt tagatagagt tatttggcga taactctttt
     3841 tgtcttactt ctgagtttag catatttttt ttgttttgta aatccatttc tttatttttc
     3901 tttacaacat aaactttttt tacttctcta taaatcttct caccagttga aacatcaatt
     3961 tctaacagtt ctctagcaat aaaattttta atatagagta aatctttttc atctgtaagt
     4021 atatcattca tttcatcaaa atccaacttt tttttggttt cttttggttt tattaaattt
     4081 cgtgaacaga aataggcttt tttttgattt gctttctttt ctaagtcttt cacaaaatac
     4141 ttcgttatat acacccccac agcttcattt ttaacagttt ctttaatctt atttattttt
     4201 ataaatccat tcgcccacaa ttccccaagc ttcttattat ctatatatgg gaccgagaac
     4261 agcaccaaat gataatggat tgctcccctc tgctggagtt cccaagttgc aatatattta
     4321 agctgttgat ttttcaagta ccttttcaat ctcttaataa ataaagtaaa ttcacgattt
     4381 gccctttcta tatcctgtat attttcttta aaagtaagag ttaaaaaggt acttttatca
     4441 tcataattac aatcaattat cctttttatt tcaaatcgct tgtcctggta atatgctttc
     4501 cttcgttgca tactttcagc ttt
//
