LOCUS       sequence-406729-        7987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inhibitor of transposase and integrase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 5124 to 5158)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pInh
FEATURES             Location/Qualifiers
     source          1..7987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     921..940
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(960..1037)
                     /gene="lacI"
                     /label=lacI promoter
     CDS             1507..2298
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFGPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2463..3008
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2909..2928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3125..3142
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3177..3263)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3292..3747)
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
     primer_bind     complement(3786..3805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3787..3805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5124..5158)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(7750..7772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7761..7777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7785..7791,7792..7809,7810..7815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7830..7851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(join(7864..7987,1..959))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
       61 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
      121 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
      181 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
      241 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
      301 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
      361 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
      421 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
      481 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
      541 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
      601 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
      661 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
      721 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
      781 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
      841 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
      901 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
      961 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     1021 cgccattcga tggtgtcaac gtaaatgcat gccgcttcgc cttcgcgccc tctggtaagg
     1081 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     1141 ggggatcaag atctcagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc
     1201 gggtgacaca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg
     1261 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     1321 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     1381 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     1441 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     1501 aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     1561 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     1621 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     1681 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     1741 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     1801 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     1861 gcaggtatct tcgggccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     1921 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     1981 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     2041 tgggcaggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     2101 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     2161 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     2221 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     2281 aaggtagtcg gcaaataaag ctttactgag ctaataacag gactgccggt aatcgcaggc
     2341 ctttttattt ctgcaactag taacaactta tatcgtatgg ggctgacttc aggtgctaca
     2401 tttgaagaga taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct
     2461 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
     2521 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
     2581 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
     2641 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
     2701 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     2761 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccgggactga gtgtcaggcg
     2821 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     2881 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
     2941 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcgaagcc
     3001 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     3061 ttccaggaaa tctccgcccc gttcgtaagc catttccgct caccgcagtc gaacgaccga
     3121 gcgtagcgag tcagtgagcg aggaagcgga atatacccct aggtctaggg cggcggattt
     3181 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3241 gactgagcct ttcgttttat ttgatgcctc tagcacgcgt accatgggat cttatccacg
     3301 gtcagaagtg accagttcgt tcttctccca ccaacgctta aggtcgaacg aagggcaagc
     3361 cttcggcgcc acctcatgat gggcgcgaag cacagcgcct tcgtacttag ccagcagtgt
     3421 gacaagcagt gagcgaaggg attgcatttg ggctggcgta aagttagcgt cgaacttacc
     3481 tttatcgtcg ataccaccaa caaggcagac gccgatagag ttgtggttgt aacccttagc
     3541 gtgagagcct acagccatct catctcgtcc tgcctccaca gtaccgtctc gcttgatgat
     3601 aaagtggtat cccacatcga gccaaccctg ctctttgtgc cactggcgaa tctcacggac
     3661 accaacattc tgacttggct tggtagccga gcagtgaaca aagattgcgt cagtagattc
     3721 acgttgttta aactgtacac gagccattat ttctttcctc ctttcctttt taatctatca
     3781 aatctcccta tagtgagtcg tattagatcc cccatcaagc ttttagattt taatgccctg
     3841 cgccatcagg tctttcgcgg ccagaaagcc atccagtttg ctttgcagcg cttcccaacc
     3901 ttcccacagc gcaccccagc tcgcaatgcc ggtacgtttg ctatccataa agccgcccag
     3961 acgcgcaatc gccatatacg cccattgcag gctgcccgct ttttctttgc gtttgcgttt
     4021 gcctttatcc agatagccca gcagttggca ttcatccggg gtcagcacgg tttccgcgct
     4081 ctggctttca acgtgttccg cttctttcag caggccctgc gcacgcagtg cttgcggcgg
     4141 agtaaaagat tcacgcagtt gcagcagacg caccgccaca aagctcagaa tgctcaccat
     4201 acgttccagg ttatccggtt cttccatacg ctgacgttcc gcacccgcac ccgttttcca
     4261 cgctttgtga aattcttcaa tgcgccaacg atgggtataa atatcaatca cacgcagcgc
     4321 ttgggccaga ctttccaccg gctcgctggt cagcagcagc catttcagcg gggtttcgcc
     4381 tttcggcgga ttaatttctt cggccagcac cgcgttcagg gtaatgttgc cctgtttcag
     4441 ggtaatacgg ccgctacgca ggctcaggct cgctttacgc gccggacggt ttttacgttt
     4501 gccacgttta tccaccacgc ctttctgcgg aatgctaatc tgatagccgc ccagttccgg
     4561 ctggtttttc aggtgatcat acagatacag gccgctttcc acatctttac gcggatgttt
     4621 gctacgcacc acaaaacgtt cgttatgggc cagtttatct tgcagatacg catgaatatc
     4681 cgcttcacga tcgcacaccg caatcacgtt gctcatcatg ctgcccattc tcagacgcga
     4741 agttgcagca gcggccagcc atttgccgct ttctttttca tccgcatccg ccggatcatc
     4801 cggacgcatc caccattctt gatgcagcag gcccacggta cgaaaggtgg tcgcttccag
     4861 cagcagcacg ctatgcaccc accaaccacg gcttttatcc tgaatgctac ccagtttgcc
     4921 cagttcttcc gccacctgat gacgatagct cagagaggtg gtatcttcaa ttgccagcag
     4981 ttccggaaat tcctgggcca gtttcacggt ctgcatggca cccgctttac gaatcgcttc
     5041 cgcgctcacg ttcggattac gaataaaacg atacgcgcct tcctgcattt tacttctcct
     5101 aattaaatta tatagcttta gctgctagca ctgtacctag gactgagcta gccgtcaatt
     5161 aggcaaatac gtattcagaa tccatgattt cgttaaggtc gaactcccct tgctcaggta
     5221 cttcgatacc tgtatcaacc atccagcgct cttcatgctc ctccagtagt ttctgaagcg
     5281 cattaccatc aatatacatt gcaaccatct gccctttaag tgccactcta agagtgaggg
     5341 tgttgtctgc atgagtacca aaagagtcgt ggattacagc gatactagtt acgcccttgt
     5401 ctaccaattc acatacggta aggataaggt gacttgcgtc atgaccgtgt acgaaattag
     5461 gtgctgctgc tcccatcata gcggcttcat ctacgatatc cgtttcaacc tgaagggaca
     5521 tcttgatatc acccatcaga caggtacgca cgcgtagcat ctcggttgcc atgatcttct
     5581 gttctaagat gaagccagta ggcagggtgt acatcaggcc ttcattacgt ttcgctgcaa
     5641 agcgtgcaag ctggcgtatc atcttcatag ctactatcgg tgccttaact acttcagaaa
     5701 tagaaggcca gattagtgcc gtcatgtagt tgtaagctgc gcccggagtc aagtaatctt
     5761 gacgatcgtc ttcaaaagga tgtaccttgt ttgccgtccg cccttctgct actgccttct
     5821 gcgcctcttt ttcctctaag tctacgatgt aatcaatcac agattcacgg caagttaagc
     5881 gagtagaacc atatggcaag gtcatcacgg gctttttggt taagctacgg gtaataccaa
     5941 tactatccca tgcgctagcc attgctcgca gttctgtacc ggacagcgtg acgctaccag
     6001 aagtaaacgt ggttgcatcg tccgcatcca tatatagcgc attcttcttg ataaccactt
     6061 gcgccaccgc cccatagata tcctgcggtg catcggaggg tttcaggtta acagctttgg
     6121 cccctacttc gtcgcgaagc atagcactat agtgctgaat gcctgaacaa gacccgtcct
     6181 gatgtactgg taggtgagtg cggaattcgt cggcccttcc ttcatccacc aaatcaaggt
     6241 attgagcata ctcaaagcac caagcgagga attcataagg tgcatcagct ttagcccatt
     6301 gggtgaatgt gagagggtct gcggcgatgt ctcgacacat atcttggaat tcctcatcta
     6361 atacgttaga cacgcgcaca tcaaaagttt tcttgtccca tccccaaagg ttagcaccat
     6421 tgatgcagaa ccatttaagc gcttctacgc cattcacagg gcgtccctcg gtaaagcgga
     6481 gtaatgcctt acctaagtcg ttagactgcg gagagagcgt gctagattgc acatagacac
     6541 ggctgcggct atccattgcg tacacgaagt aaatggattc aaaggcgcta tatttacggg
     6601 cctgtcctac catgcgaaca acggcggcgg actttgaacc gcgcttagtt tctgcggtat
     6661 ataggcgcgc gcattcgcct ttccagttaa tgaattgttg ccactgctca ggtgatagca
     6721 tctctttcag ttcacgaccg cgcaggtgtt ggaattcaac aggtaccggg ttagctggct
     6781 tgttctcctt gtcaatcagt ggcttgaagg aaggtacacc ataaccaagg tctaagcgga
     6841 ttgcttcttc aataactgct aatacatcct tgttgatttg ccattgtgta ttttgtaatg
     6901 cgttgatagc cttataaacc tttggcattt gcttttgagt caacttgcgt acatgctcac
     6961 ggttaccttt tacaagacgg atacggctag ctaccttctc agtatggaac cctccattaa
     7021 atggagttct ccaaggacga ggagggatta cgcaaggggc ataagctggg cttaattgcg
     7081 ctacgtgctc tttgaatgcg ctaatccact ggcctacact ttcagaagtt tgtaagtagt
     7141 aaatagtctt tccgccataa gtgcgcatag cacgcataaa tacaggttca ccattataga
     7201 aaacgctacc ttctaagatt tcaagcaagg tagtaccaat ctgcaattga gtttcttttg
     7261 gccacgcctc ccaacggtca aagtccgcgt ccttttctgc aactgatttt tcagcaacta
     7321 cagctacgtt atgagcgtga cgatatgact tagtacggct agccttgagt gacttcttaa
     7381 ccttctcaaa gtatttagcg gcgtgacctt ctagcttaga aaagcgcact tggtcttcaa
     7441 tgcgttctgc tacactcatt gcaatagcct gaagggtagc atccgtattc agcatatcca
     7501 taacaacttt catagtgatg tatgctgcaa cttcattttc tacacattgt aagaaagcca
     7561 atgcgcgagg tgcacgacct ttcttacctt cgtactcttc tttataagcc tgaatgcctt
     7621 cagccatagg tgcaataagt tctgacaaca ggcggcggtt ccatgctgtg tcgctctcgc
     7681 tacctgctgc aatctggcgt tgttgatctg cttcgaagcg acgaatgcca ccattaaaca
     7741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7801 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
     7981 tgagacg
//
