LOCUS       sequence-406733-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting AnkyrinB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AnkyrinB-GFP/pEGFP-N1
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        175..478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        479..682
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     633..653
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     728..744
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     729..745
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6314..6323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6320..6322,6323..6325,6326..7039)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6320..6322,6323..6325,6326..7036)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6365..6386)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6626..6645)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6973..6994
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7162..7283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7199..7218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7253..7272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7290..7745)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7427..7448)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7639..7658
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7772..7876
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7878..8235
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7878..8069
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7901..7921)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8086..8221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8270..9064
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8270..9064
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8324..8343)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8934..8953
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9252..9271)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9296..9343
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9672..10260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10161..10180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
      121 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      181 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      241 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      301 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      361 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      421 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      481 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      541 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      601 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      661 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      721 tcagatctcg aggtcgacgg tatcgataag cttgatatcg aattcgggca tcaaaccgtt
      781 caaaatgatg aacgaagatg cagctcagaa aagcgacagt ggagagaagt tcaacggcag
      841 tagtcagagg agaaaaagac ccaagaagtc tgacagcaat gcaagcttcc tccgtgctgc
      901 cagagcaggc aacctggaca aagttgtgga atatctgaag gggggcatag acatcaatac
      961 ctgcaatcag aatggactca acgctctcca tctggctgcc aaggaaggcc acgtggggct
     1021 ggtgcaggag ctgctgggaa gagggtcctc tgtggattct gccactaaga agggaaatac
     1081 cgctcttcac attgcatctt tggctggaca agcagaagtt gtcaaagttc ttgttaagga
     1141 aggagccaat attaatgcac agtctcagaa tggctttact cctttataca tggctgccca
     1201 agagaatcac attgatgttg taaaatattt gctggaaaat ggagctaatc agagcactgc
     1261 tacagaggat ggctttactc ctctagctgt ggcactccag caaggacaca accaggcggt
     1321 ggccatcctc ttggagaatg acaccaaagg gaaagtgagg ctgccagctc tgcatattgc
     1381 cgctaggaaa gacgacacca aatctgccgc acttctgctt cagaatgacc acaatgctga
     1441 cgtacaatcc aagatgatgg tgaataggac aactgagagt ggttttaccc ctttgcacat
     1501 agctgcacat tacggaaatg tcaacgtggc aactcttctt ctaaaccggg gagctgctgt
     1561 ggacttcaca gccaggaatg gaatcactcc tctgcatgtg gcttccaaaa gaggaaatac
     1621 aaacatggtg aagctcttac tggatcgagg cggtcagatc gatgccaaaa ctagggatgg
     1681 gttgacacca cttcactgtg ctgcacgaag tgggcatgac caagtggtgg aacttctgtt
     1741 ggaacggggt gcccccttgc tggcaaggac taagaatggg ctgtctccac tacacatggc
     1801 tgcccaggga gaccacgtgg aatgtgtgaa gcacctgtta cagcacaagg cacctgttga
     1861 tgatgtcacc ctagactacc tgacagccct ccacgttgct gcgcactgtg gccactaccg
     1921 tgtaaccaaa ctccttttag acaagagagc caatccgaac gccagagccc tgaatggttt
     1981 tactccactg cacattgcct gcaagaaaaa ccgcatcaaa gtcatggaac tgctggtgaa
     2041 atatggggct tcaatccaag ctataacaga gtctggcctc acaccaatac atgtggctgc
     2101 cttcatgggc cacttgaaca ttgtcctcct tctgctgcag aacggagcct ctccagatgt
     2161 cactaacatt cgtggtgaga cggcactaca catggcagcc cgagccgggc aggtggaagt
     2221 ggtccgatgc ctcctgagaa atggtgccct tgttgatgcc agagccaggg aggaacagac
     2281 acctttacat attgcctccc gcctgggtaa gacagaaatt gtccagctgc ttctacaaca
     2341 tatggctcat ccagatgcgg ccactacaaa tgggtacaca ccactgcaca tctctgcccg
     2401 ggagggccag gtggatgtgg catcagtcct attggaagca ggagcagccc actccttagc
     2461 taccaagaag ggttttactc ccctgcatgt agcagccaag tatggaagcc tggatgtggc
     2521 aaaacttctc ttgcaacgcc gtgctgccgc agattctgca gggaagaacg gccttacccc
     2581 gctccatgtt gctgctcatt atgacaacca gaaggtggcg ctgctgttac tggagaaggg
     2641 tgcttcccct catgccactg ccaagaatgg ctatactccg ttacatattg ctgccaagaa
     2701 gaatcaaatg cagatagctt ccacactcct gaactatgga gcagagacaa acattgtgac
     2761 aaagcaagga gtaactccac tccatctggc ctcgcaggag gggcacacag atatggttac
     2821 cttgcttctg gataagggag ccaatatcca catgtcaact aagagtggac tcacatcctt
     2881 acaccttgca gcccaggaag ataaagtgaa tgttgctgat attctcacca agcatggagc
     2941 tgatcaggat gctcatacaa agcttggtta cacaccttta attgtggcct gtcactatgg
     3001 aaatgtgaaa atggtcaact ttcttctgaa gcagggagca aatgttaacg caaaaaccaa
     3061 gaacggctac acgcctttgc accaggccgc tcagcagggt cacacgcaca tcatcaacgt
     3121 cctgctccag catggggcca agcccaacgc caccactgcg aatggcaaca ctgccttggc
     3181 gattgctaag cgtctgggct acatctccgt ggtcgacacc ctgaaggttg tgactgagga
     3241 ggtcaccacc accaccacaa ctattacaga aaaacacaaa ctaaatgtac ctgagacgat
     3301 gactgaggtt cttgatgttt ctgatgaaga gggtgatgac acaatgactg gtgatggggg
     3361 agaatacctt aggcctgagg acctaaaaga actgggtgat gactcactac ccagcagtca
     3421 gttcctggat ggtatgaatt acctgcgata cagcttggag ggaggacgat ctgacagcct
     3481 tcgatccttc agttccgaca ggtctcacac tctgagccat gcctcctacc tgagggacag
     3541 tgccgtgatg gatgactcag ttgtgattcc cagtcaccag gtgtcaactc tagccaagga
     3601 ggcagaaagg aattcttatc gcctaagctg gggcactgag aacttagaca acgtggctct
     3661 ttcttctagt cctattcatt caggtttcct ggttagtttt atggtggatg cccgaggtgg
     3721 tgctatgcga ggatgcagac acaatgggct ccgaatcatt attccacctc ggaaatgtac
     3781 tgctccaacg cgagtcacct gccgactggt caagcgccac agactggcaa caatgcctcc
     3841 aatggtggaa ggagaaggcc tggccagtcg cctgatcgaa gttggacctt ctggtgctca
     3901 gttccttggg cctgtgatcg tggagatccc tcactttgcg gcccttcgag gaaaggaaag
     3961 ggaactggtg gtcctgcgca gtgagaatgg ggacagctgg aaagagcatt tctgtgacta
     4021 cactgaagat gaattgaatg aaattcttaa cggcatggat gaagtactgg atagcccaga
     4081 agacctagaa aagaaacgaa tctgccgcat catcacccga gacttcccac agtactttgc
     4141 agtggtgtct cgtatcaaac aggacagcaa tctgattggc ccagaaggag gtgtactgag
     4201 cagcacagtg gtgccccagg tgcaggccgt cttcccagag ggggcactca ccaagcggat
     4261 ccgtgtaggc ctgcaggctc aacctatgca cagtgagctg gttaagaaga tcctaggcaa
     4321 caaagctacc ttcagcccta tagtcacttt ggaacctaga agaagaaaat tccacaaacc
     4381 aattaccatg accattcctg tccccaaagc ttcaagtgat gtcatgttga atggttttgg
     4441 gggagatgca ccaaccttaa gattactatg cagcataaca ggtggaacca cccctgccca
     4501 gtgggaagat attacaggaa ctacgccatt aacatttgtc aatgaatgtg tttcctttac
     4561 aacaaacgtg tctgccaggt tctggctgat agattgtcga cagatccagg aatccgttac
     4621 ttttgcatca caagtataca gagaaattat ctgcgtacct tatatggcca aatttgtagt
     4681 gtttgccaaa tcacatgacc ccattgaagc caggttgagg tgtttctgca tgactgatga
     4741 taaagtggat aagacccttg aacaacaaga aaattttgct gaggtggcca gaagcaggga
     4801 tgtggaggtg ttagaaggaa aacccatcta cgttgattgt ttcggcaact tggtaccatt
     4861 aactaaaagt ggccagcatc atatattcag tttttttgcc ttcaaagaaa atagacttcc
     4921 tctatttgtc aaggtacgcg atacgactca ggaaccttgc ggacgactat catttatgaa
     4981 ggagccaaaa tccacgagag gcctggtgca tcaagctatt tgcaacttaa acatcacttt
     5041 gccgatttat acaaaggaat cagagtcaga tcaagaacag gaggaagaga tcgatatgac
     5101 atcagaaaaa aatccacagg atgagcagga acggatcgag gaaaggctgg cttatattgc
     5161 tgatcacctt ggcttcagct ggacagaatt agcaagagaa ctggatttca ctgaggagca
     5221 aattcatcaa attcgaattg aaaatcccaa ctctcttcaa gaccagagtc atgcactgtt
     5281 gaagtactgg ctagagaggg atgggaaaca tgctacagat accaacctcg ttgaatgtct
     5341 caccaagatc aaccgaatgg atattgttca tctcatggag accaacacag aacctctcca
     5401 ggagcgcatc agtcatagtt atgcagaaat tgaacagacc attacactgg atcatagtga
     5461 agggttctcg gtacttcaag aggagttatg cactgcacag cacaagcaga aagaggagca
     5521 agctgtttct aaagaaagtg agacctgcga tcaccctcct atcgtctcag aggaagacat
     5581 ttctgttggt tattccactt ttcaggatgg cgtccccaaa actgaggggg acagctcatc
     5641 aacagcactc tttccccaaa ctcacaagga gcaagttcaa caggatttct cagggaaaat
     5701 gcaagacctg cctgaagagt catctctgga atatcagcag gaatattttg tgacaactcc
     5761 aggaacagaa acatcagaga ctcagaaggc tatgatagta cccagctctc ccagcaagac
     5821 acctgaggaa gttagcaccc ctgcagagga ggagaagctg tacctccaga ccccaacatc
     5881 cagcgagcgg ggaggctctc ccatcataca agaacccgaa gagccctcag agcacagaga
     5941 ggagagctct ccgcggaaaa ccagcctcgt aatagtggag tctgccgata accagcctga
     6001 gacctgtgaa agactcgatg aagatgcagc ttttgaaaag ggagacgata tgcctgaaat
     6061 acccccagaa acagtcacag aagaagaata cattgatgag catggacaca ccgtggtaaa
     6121 gaaggttact aggaaaatca ttaggcggta tgtatcctct gaaggcacag agaaagaaga
     6181 gattatggtg cagggaatgc cacaggaacc tgtcaacatc gaggaagggg atggctattc
     6241 caaagttata aagcgtgttg tattgaagag tgacaccgag cagtcagagg acaacaatga
     6301 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6361 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     6421 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     6481 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     6541 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     6601 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     6661 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6721 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     6781 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     6841 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6901 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6961 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     7021 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     7081 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     7141 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     7201 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7261 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     7321 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7381 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7441 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7501 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7561 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7621 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7681 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7741 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7861 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     7921 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7981 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8041 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8101 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8221 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     8281 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     8341 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     8401 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     8461 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     8521 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     8581 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     8641 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     8701 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     8761 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     8821 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8881 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8941 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     9001 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     9061 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     9121 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     9181 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     9241 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     9301 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     9361 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     9421 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     9481 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     9541 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9601 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9661 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9721 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9781 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9841 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9901 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9961 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10021 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10081 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10141 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10201 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
//
