LOCUS       sequence-406744-        8050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat beta1b calcium channel auxillary subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8050)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: cacnb1b pcDNA3
FEATURES             Location/Qualifiers
     source          1..8050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3603..3621)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3604..3621)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3641..3658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3647..3871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3917..4345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4004..4023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4214..4235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4354..4374)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4359..4688
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4539..4674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4601..4620
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4755..5549
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4809..4828)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5419..5438
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5723..5844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5760..5779)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5814..5833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5893..5909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5893..5909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5906..5928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5917..5933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5941..5947,5948..5965,5966..5971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5986..6007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6124..6141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6295..6883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6375..6394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7054..7845,7846..7914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7677..7696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7915..8019)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcggcacga gagcgtgcag
      961 ctgggcgact gcacccggtg ctggctgcgc gacgccgcgc tgctctcggc tcggacggcc
     1021 tctcccatgc gctgagggcc cccggctgtg ccgggagggc ggccggaccg gaggatcctc
     1081 tccatggtcc agaagagcgg catgtcccgg ggcccttacc caccttccca agagatccct
     1141 atggaggtct tcgaccccag cccacagggc aaatacagca agaggaaagg gcggttcaaa
     1201 aggtcagatg ggagcacctc ctcagatacc acatccaaca gctttgtccg tcagggctca
     1261 gcagagtcct acacgagccg gccgtcagac tccgacgtgt ccctggagga ggaccgggaa
     1321 gccttaagga aggaggcaga gcgccaggcc ttagcccagc tcgagaaagc caagaccaaa
     1381 ccagtggcct ttgctgttcg gacaaatgtt ggctacaatc cgtctccagg agatgaggtg
     1441 ccagtgcagg gagtggccat cacctttgag cccaaggact tcctgcacat caaggagaaa
     1501 tacaataatg actggtggat cgggaggctg gtgaaggaag gctgtgaggt tggtttcatc
     1561 cccagccctg tcaaactgga cagccttcgc ctgctgcagg aacagacgct gcgccaaaac
     1621 cgcctcagct ccagcaagtc aggtgacaac tccagttcca gtctgggaga tgtggtgact
     1681 ggcacccgcc gccccacacc ccccgccagt gccaaacaga agcagaagtc gacagagcac
     1741 gtgcccccct atgacgtggt gccttccatg aggcccatca tcctggtggg accatcgctc
     1801 aagggctatg aggtaactga catgatgcag aaggcgttgt ttgacttctt gaagcatcgg
     1861 tttgatggca ggatttccat cacccgggta acggctgaca tttccctggc caagcgctcg
     1921 gtcctcaaca accccagcaa acacattatc atcgagcgct ccaacactcg ctccagcctg
     1981 gctgaggtac agagtgaaat cgagcggatc ttcgagctgg cccggacctt gcagctggtt
     2041 gccctggacg ccgacaccat caaccaccca gcccagctct ctaaaacctc gctggccccc
     2101 atcattgttt acatcaagat cacatccccc aaggtactgc agaggctcat caaatcccga
     2161 gggaagtctc aatccaaaca cctcaatgtc caaatagcag cctcggagaa gctggcacag
     2221 tgtccccccg aaatgtttga cataatcctg gacgagaacc aattggaaga tgcctgcgag
     2281 cacctggcgg agtacttgga agcctactgg aaggccacac acccgcctag cagcacgcca
     2341 cccaatccgc tgctgaaccg caccatggct accgccgctc tggctgccag ccctgccccc
     2401 gtctccaacc tccagggacc ctaccttgct tccggggacc agccgctgga ccgggccact
     2461 ggggagcatg ccagtgtgca cgagtacccc ggggagctgg gccagccccc aggcctttac
     2521 cccagcaacc acccacctgg ccgggcaggc accctgcggg cgctatcccg ccaagacacc
     2581 tttgatgctg acacccccgg cagccgaaac tctgcctaca cggagccagg agactcgtgt
     2641 gtggacatgg agacagaccc ctcagagggc ccagggcctg gagaccctgc agggggaggt
     2701 acaccaccag ctcggcaggg ctcctgggaa gaggaggaag attatgagga ggagatgacc
     2761 gacaacagga accggggccg gaataaggcc cgctactgtg cggagggtgg tgggccggtt
     2821 ctggggcgca ataagaatga gctggagggc tggggacaag gcgtctacat ccgctgagag
     2881 gcaggggcag ttctgggaga gaagaggctc tgaactcggg gaggggaggg cagaggggcg
     2941 caccacccaa gatgtgtctt gcctccaggg ggcgctttgt ccctcatttt cagatgcctt
     3001 tgctcaaagc ttggggtttc tttggtggtt atcattccca actcctggga ggtccttaat
     3061 ccccagctgt aggtttttcc ctaaactgct gtgggtggat gggggaatac ctactttctg
     3121 aagtgccccc tttccccatc tttggggctt tctccctccc agagaaaagg tgcacttcct
     3181 taacttttta ctcggggctg taagcggagg tcctaattgg ggtggggtgg aggggtttct
     3241 tctaccaacg caggggtact tgggaaaggt tgggtgcttt tcctgaagag aacccgctga
     3301 atcttccaca aaccccagac ccgggatccc ccaccatatc cattccataa cctagtttcc
     3361 tagcagcatt ctctggtggg tgaaagccac agcttggaaa cccatgaaga gcttgctttg
     3421 gggagcagag ctgggtgcaa actgacgggc atcagtgtgt gctctctgcc ctccatggtc
     3481 atggcaagaa agcagggcct agaaacttga ggagtgagca actcaagatg ctgcctcgtg
     3541 ccgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccct
     3601 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     3661 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3721 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3781 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3841 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3901 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3961 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4021 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4081 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4141 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4201 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4261 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4321 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4441 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4501 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4561 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4621 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4681 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4741 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4801 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4861 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4921 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4981 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5041 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5101 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5161 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5221 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5281 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5341 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5401 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5461 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5521 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5581 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5641 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5701 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5761 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5821 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5881 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5941 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6001 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6061 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6721 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7021 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8041 acctgacgtc
//
