LOCUS       sequence-406752-        8498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse calcium channel alpha2delta-3 with a single 
            Bungarotoxin binding site motif inserted after aa595.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8498)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: cacna2d3 BBS pMT2
FEATURES             Location/Qualifiers
     source          1..8498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4553..5116
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    5303..5437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5340..5359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5394..5413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6110..6127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6281..6869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6361..6380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7040..7831,7832..7900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7663..7682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7901..8005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8073..8091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8129..8151)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8251..8270
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8464..8486
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8478..8495
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8479..8495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attgcggccg
     1081 ctccagtcgc cgccgccgcc gccgccgcca ggatggccgg gccgggctcg ctgtgctgcg
     1141 cgtcccgggg ggcctcggcg ctcctagcca ccgcgcttct ctacgccgct ctgggggacg
     1201 tggtgcgctc cgagcagcag atcccgctct ccgtagtgaa gctgtgggcc tccgcttttg
     1261 gtggggagat aaaatccatc gctgccaagt actcgggctc ccagcttctg caaaagaaat
     1321 acaaagagta tgagaaggac gttgccattg aagaaatcga cggtctccaa ctggtgaaga
     1381 agttggctaa aatcatggag gagatgttcc acaagaagtc cgaggcagtg aggcgtcttg
     1441 tggaggctgc agaggaggca cacctgaaac atgaatttga tgctgacttg cagtatgaat
     1501 acttcaatgc tgtgctgatc aacgagaggg acaaagacgg gaactttttg gaattgggaa
     1561 aggaattcat cttagccccc aatgaccatt ttaataattt gcctgtgaac atcagtctga
     1621 gtgatgtgca agtgccaacg aacatgtaca acaaagatcc tgccattgtc aatggagtgt
     1681 attggtctga atctctaaac aaagtttttg tggataactt tgatcgggat ccgtctctca
     1741 tatggcagta ctttggaagt gcaaagggct ttttcagaca gtacccaggg attaaatggg
     1801 aaccagatga gaatggagtc attgcctttg actgcaggaa cagaaaatgg tacatccagg
     1861 cagcgacttc tccaaaggat gttgtcattt tggtggacgt cagtgggagc atgaaaggac
     1921 tccgcttgac catcgccaag caaacagtgt cctcaatact ggatactctg ggtgatgatg
     1981 acttcttcaa catcatcacg tataacgaag agcttcacta tgtggaacct tgtctgaacg
     2041 gaacactggt tcaagctgac aggaccaaca aggagcactt cagggagcat ttggacaaac
     2101 tttttgccaa agggattgga atgctggata tcgcactgaa cgaggccttc aacatactga
     2161 gcgatttcaa ccacactgga caaggaagca tttgcagcca ggccatcatg ctcataactg
     2221 atggggcagt ggacacctat gataccatct ttgcaaaata taattggcca gaccgaaagg
     2281 ttcgaatctt tacttacctc attggacggg aggctgcttt tgccgacaat ctcaagtgga
     2341 tggcttgtgc taacaaagga tttttcaccc agatctccac cttggctgat gtgcaggaaa
     2401 atgtcatgga atacctccat gtgcttagcc gacctaaagt cattgaccag gagcatgatg
     2461 tggtgtggac cgaagcgtac attgacagca ccctccctca ggctcaaaag cttgctgatg
     2521 atcagggcct cgtcttgatg accacggtgg ccatgcctgt gtttagtaag cagaacgaaa
     2581 ctaggtcaaa gggcattctt ctgggtgtgg ttggcacaga tgtcccagtt aaagagcttc
     2641 tgaagaccat ccccaaatac aagttaggaa ttcatggtta cgccttcgcc atcacgaata
     2701 atggatatat cctgacgcac ccggagctca ggccactgta tgaagaagga aaaaaacgga
     2761 ggaaacccaa ctacagtagt gtggatctct ctgaagtcga gtgggaagac cgggatgatg
     2821 tgttacgaaa tgccatggta aatcggaaga ctgggaaatt ctccatggaa gtgaagaaga
     2881 ccgtggacaa agggaaatgg agatactacg agagctccct ggagccctac cctgaccggg
     2941 ttttggtgat gaccaatgac tactactata cagacatcaa gggtactcct ttcagtttag
     3001 gtgtggcgct ctccaggggc catgggaaat acttcttccg agggaatgta accattgaag
     3061 aaggcctcca tgacttagaa catcctgacg tgtccttggc agatgaatgg tcctactgca
     3121 acactgacct gcacccagag caccgccatc tatctcaact ggaagccatt aagctctacc
     3181 tcaaaggcaa ggagcctctg cttcaatgtg acaaagaatt gattcaagaa gtcctttttg
     3241 atgctgtggt gagcgcccct attgaagcct attggacgag cctggccctc aacaaatctg
     3301 agaattctga caagggtgta gaggtcgcct tcctcggcac tcgcacaggc ctctcaagaa
     3361 tcaacctgtt tgtgggggcc gaacaactca ccaatcagga ctttctgaag gctggagaca
     3421 aagagaacat ttttaatgcc gatcatttcc ctctctggta cagaagagct gccgagcaga
     3481 tcgcaggaag ctttgtctat tccatcccct tcagcacagg aacagtcaac aaaagcaatg
     3541 tggtgacagc aagtacctcc atccagctcc tggatgagcg gaaatctccc gtggtggcag
     3601 ctgtaggcat tcagatgaaa cttgaattct tccaaaggaa gttctggact gccagcagac
     3661 agtgtgcctc cctggatggc aaatgctcca taagctgcga tgacgagact gtgaactgtt
     3721 accttataga caataacgga ttcattctgg tgtctgaaga ctacacacag actggagatt
     3781 tttttggtga ggtggaagga gctgtcatga acaagttgtt aacaatgggt tcctttaaaa
     3841 gaataacctt gtacgactac caagccatgt gtagagccaa caaggagagc agtgacagtg
     3901 cccatggact tctggacccc tataaggcct ttctctctgc agccaagtgg ataatgacgg
     3961 aacttgtctt gttcctggtg gagtttaacc tgtgcagttg gtggcactcc gacatgacag
     4021 ctaaagccca gaaactgaaa cagaccctgg aaccttgtga tactgaatac ccagcctttg
     4081 tttctgaacg caccatcaag gagaccacag ggaacattgc ttgcgaagac tgctccaagt
     4141 ccttcgtcat ccagcaaatc ccgagtagca atctgttcat ggtggtggtg gacagtagct
     4201 gtctctgtga gtccgtggct cctataacca tggcacccat tgaaatcagg tataatgaat
     4261 cccttaagtg tgaacggtta aaggctcaga agatcagacg acgtccagaa tcctgccacg
     4321 gcttccatcc tgaggagaat gcaagagagt gtgggggtgc atcgagtctt caggcccagg
     4381 cggccttgct gctgctgccc ctggtttcga gtctcttctc aaggtgagcg gccgcactag
     4441 tcccgggtac ctcgaggaat tccggggggg acagctcagg gctgcgattt cgcgccaaac
     4501 ttgacggcaa tcctagcgtg aaggctggta ggattttatc cccgctgcca tcatggttcg
     4561 accattgaac tgcatcgtcg ccgtgtccca aaatatgggg attggcaaga acggagacct
     4621 accctggcct ccgctcagga acgagttcaa gtacttccaa agaatgacca caacctcttc
     4681 agtggaaggt aaacagaatc tggtgattat gggtaggaaa acctggttct ccattcctga
     4741 gaagaatcga cctttaaagg acagaattaa tatagttctc agtagagaac tcaaagaacc
     4801 accacgagga gctcattttc ttgccaaaag tttggatgat gccttaagac ttattgaaca
     4861 accggaattg gcaagtaaag tagacatggt ttggatagtc ggaggcagtt ctgtttacca
     4921 ggaagccatg aatcaaccag gccacctcag actctttgtg acaaggatca tgcaggaatt
     4981 tgaaagtgac acgtttttcc cagaaattga tttggggaaa tataaacttc tcccagaata
     5041 cccaggcgtc ctctctgagg tccaggagga aaaaggcatc aagtataagt ttgaagtcta
     5101 cgagaagaaa gactaacagg aagatgcttt caagttctct gctcccctcc taaagctatg
     5161 catttttata agaccatggg acttttgctg gctttagatc ataatcagcc ataccacatt
     5221 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5281 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     5341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5401 gtccaaactc atcaatgtat cttatcatgt ctggatcccc ggccaacggt ctggtgaccc
     5461 ggctgcgaga gctcggtgta cctgagacgc gagtaagccc ttgagtcaaa gacgtagtcg
     5521 ttgcaagtcc gcaccaggta ctgatatccc accaaaaagt gcggcggcgg ctggcggtag
     5581 aggggccagc gtagggtggc cggggctccg ggggcgaggt cttccaacat aaggcgatga
     5641 tatccgtaga tgtacctgga catccaggtg atgccggcgg cggtggtgga ggcgcgcgga
     5701 aagtcgcgga cgcggttcca gatgttgcgc agcggcaaaa agtgctccat ggtcgggacg
     5761 ctctggccgg tgaggcgtgc gcagtcgttg acgctctaga ccgtgcaaaa ggagagcctg
     5821 taagcgggca ctcttccgtg gtctggtgga taaattcgca agggtatcat ggcggacgac
     5881 cggggttcga accccggatc cggccgtccg ccgtgatcca tgcggttacc gcccgcgtgt
     5941 cgaacccagg tgtgcgacgt cagacaacgg gggagcgctc cttttggctt ccttccaggc
     6001 gcggcggctg ctgcgctagc ttttttggcg agctcgaatt aattctgcat taatgaatcg
     6061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8041 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8101 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8161 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8221 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8281 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     8341 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8401 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8461 tttcccagtc acgacgttgt aaaacgacgg ccagtgcc
//
