LOCUS       sequence-406757-       11986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and an E1658K mutation in the P loop of Domain 
            IV which abolishes trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.2 HA EIVK (E1658K) pCAGGS
FEATURES             Location/Qualifiers
     source          1..11986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3472..3498
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3502..3528
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9008..9027)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9073..9128
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9127..9146)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9489..9505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9489..9505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9502..9524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9513..9529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9537..9543,9544..9561,9562..9567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9582..9603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9662..9857
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9708..9843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9863..9997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9900..9919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9954..9973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10065..10082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10236..10824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10316..10335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10995..11786,11787..11855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11618..11637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11856..11960)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggggc gccggggggg gggggggggg gggggggggg
      541 ggggggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaggg
     1741 taccgggccc cccctcgagg tcgaagcttg atcatggtcc gcttcgggga cgagctgggc
     1801 ggccgctatg ggggcgcggg cggcgcggag cgggcccggg gcggcggggc cggcggggct
     1861 ggcggcccgg gccccggggg gctgccgccg ggccagcggg tcctctacaa acagtcgatc
     1921 gcgcagcgcg cgcggaccat ggcgctgtac aaccccatcc cggtcaagca gaactgcttc
     1981 accgtcaacc gctcgctctt cgtcttcagc gaggacaacg tcgtccgcaa atacgccaag
     2041 cgcatcaccg agtggcctcc gttcgagtac atgatcctgg ccaccatcat cgccaactgc
     2101 atcgtgctgg ccctggagca gcacctccct gacggggaca agacgcctat gtccgagcgc
     2161 ctggatgaca ccgagcccta cttcattggg atcttctgct tcgaggcggg gatcaagatc
     2221 ctggccctgg gctttgttct gcacaagggc tcctatctgc ggaacggctg gaacgtcatg
     2281 gacttcgtgg tagtcctcac ggggattctt gccacagccg gaaccgactt cgacctgcga
     2341 acgctgaggg ctgtgcgtgt gctaaggccc ctgaagctgg tgtctgggat tccgagcttg
     2401 caagtggtgc tcaagtccat catgaaggcc atggtcccgc tgctgcagat cgggctgctg
     2461 ctcttcttcg ccatcctcat gtttgccatc atcggcctcg agttctacat gggcaagttc
     2521 cacaaggcct gcttccccaa cagcacagac ccagatcccg tgggtgactt cccctgtggc
     2581 aaggaggccc cggcccggct ctgtgaggga gacacggagt gccgggagta ctgggccgga
     2641 cccaactttg gtatcaccaa ctttgacaac atcctgtttg ccatcttgac cgtgttccag
     2701 tgcatcacca tggagggctg gactgacatc ctctacaata caaacgatgc agccggcaac
     2761 acgtggaact ggctctactt catccctctc atcatcatcg gctccttctt catgctcaac
     2821 ctggtgctgg gtgtgctctc aggagagttt gctaaggagc gcgagagagt ggagaaccgc
     2881 cgcgccttcc tgaagctccg gcggcagcag cagattgagc gggagctcaa cgggtacctg
     2941 gagtggatct tcaaggctga ggaagtcatg ctggctgagg aggacaggaa cgcagaggag
     3001 aagtcccctc tggatgcagt gttgaagaga gcagctgcca agaagagcag aagcgacctg
     3061 atccaggccg aggaggggga gggccggctc acaggcctct gtgctcccgg ttccccgttc
     3121 gcccgtgcca gcctgaaaag cgggaagacg gagagctcgt cgtacttccg gaggaaggag
     3181 aagatgttcc gcttcttcat ccggcgcatg gtgaaggcac agagcttcta ctggacggtg
     3241 ctgtgtgtgg tggcactgaa cacactgtgc gtggccatgg tgcactacaa ccagccgcag
     3301 cggctcacca cggcattgta ctttgcagag tttgttttcc tgggtctctt cctcacagag
     3361 atgtccctga agatgtatgg cctggggccc aggagctact tccggtcctc cttcaactgt
     3421 tttgactttg gggtcatcgt gggcagtatc tttgaagtgg tctgggctgc ttacccctac
     3481 gacgtccccg actacgccgg ctacccgtac gacgtcccgg actacgccgg cgtcgacgtc
     3541 aagccaggga cctccttcgg gatcagtgtt ctgcgggctc tgcgcttact gagaatcttc
     3601 aaggtcacca aatactggaa ctccctacgg aacctggtgg tgtctctgct caactccatg
     3661 aagtccatca tcagcctcct cttcctgctc ttcctgttca ttgttgtctt tgctctgctg
     3721 gggatgcagc tgtttggagg ccagttcaac tttaaagacg agaccccaac gaccaacttt
     3781 gacaccttcc ctgctgctat cctcaccgtc ttccagatcc tgaccggaga ggactggaat
     3841 gcggtcatgt accacgggat cgagtcgcaa ggtggcgtca gcagaggaat gttctcctcc
     3901 ttctacttca ttgtcctcac tctgtttggg aactacaccc tgctgaatgt cttcctggcc
     3961 attgctgtgg acaaccttgc caatgctcag gagctgacca aggatgaaga ggagatggaa
     4021 gaagcagcca atcagaaact cgccttgcag aaggccaagg aagtggctga agtcagcccc
     4081 atgtctgctg cgaacatctc cattgccgcc aggcagcaga actcggccaa agcgcgctcg
     4141 gtgtgggagc agcgagccag ccagctgcgg ctgcagaacc tgagggccag ctgcgaggcg
     4201 ctgtacagcg agatggaccc cgaggagagg ctgcgctacg ccaccgcgcg ccacctgcgg
     4261 cccgacgtga agacgcacct ggaccggccg ctggtggttg agcccggccg cgacgctccg
     4321 cggggccccc cgggggggaa gtcccggccg gatggctcgg aggcccccga gggtgcggat
     4381 cccccgcgca ggcatcaccg gcaccgagac aaggacaagg ctcccgccac ggtcccctcg
     4441 gcgggggaac aggacagagc cgaagctctg agggcagagg gcggggagct ggggccccgc
     4501 gaggagcggg ggcggccacg ccgcagccgc agcaaggagg ccccgggcgc cccggaggtg
     4561 cgcagcgatc gcggccgtgg cccgtgtcct gaaggtggtc ggcgccacca ccggcgcggg
     4621 tcaccggagg aggcagctga gagggagccc cgccgccacc gcgcgcaccg gcacgggccc
     4681 gacccgggca aggagggccc agcgtccggg accaggggcg agcggcgcgc acgacaccgc
     4741 acgggccccc gggcgtgccc ccgggaggct gagagcagcg aggagcctgc gcggcggcac
     4801 cgggctcggc acaaggcgcc gcccacccag gagaccgcgg agaaggacaa ggaggctgcc
     4861 gagaaggggg gcgaggccac ggaggccgag aaggacaagg aggctcggaa ccaccagccc
     4921 aaggagctgc cctgtgacct ggaggccatc ggcatgctgg gagtgggcgc tgtgcatacg
     4981 ctgcccagca cctgtctgca gaaggtggag gagcagccag aggatgcgga caaccagcgg
     5041 aacgtcaccc gcatgggtag tcagcccccg gacaccagca ccactgtgca cattcctgtg
     5101 acgctgaccg gcccccccgg ggagaccacg gtagtgccca gtggaaacgt ggacctggaa
     5161 agtcaagcag aggggaagaa ggaggtggaa accagcgatg tgatgaggag cggccctcgg
     5221 cccattgtcc cgtacagttc catgttctgt ttaagcccaa ccaacctgct gcgccgctgc
     5281 tgccactaca ttgtgaccat gcgatacttc gagatggtca tcctcgtggt catcgccctg
     5341 agcagcatcg ccctggctgc cgaggacccc gtgagaacag actctcccag gaataatgct
     5401 ctgaagtaca tggactacat cttcacaggc gtcttcacct tcgagatggt gataaagatg
     5461 atcgatttgg gactgctgct acaccctgga gcctatttcc gggatctgtg gaacattctg
     5521 gacttcattg tggtcagtgg ggccctggtg gcatttgctt tctcaggatc caaagggaaa
     5581 gacatcagca ccatcaaatc cctgagagtc ctgcgtgtgc tgcggccact caagaccatc
     5641 aagcggctgc ccaagctcaa ggccgtgttt gactgtgtgg tgaactcact gaaaaacgtc
     5701 ctcaacatcc tgatcgtcta catgctcttc atgttcatct ttgccgtcat tgccgtgcag
     5761 ctcttcaaag ggaagttctt ctactgcacg gacgaatcca aggagctgga gcgtgactgc
     5821 aggggtcagt atttggatta tgagaaggag gaagtggaag ctcaaccgag gcagtggaag
     5881 aaatatgact tccactatga caacgtgctc tgggctctgc taacgctctt cacggtgtcc
     5941 acaggggaag ggtggcccat ggtgctgaaa cactctgtgg atgccaccta cgaggagcag
     6001 ggcccgagcc ccgggtaccg catggagctc tccatcttct acgtggtcta ctttgtggtc
     6061 tttcccttct tctttgtcaa catcttcgtg gccttgatca tcatcacctt ccaggagcag
     6121 ggggacaagg tgatgtccga gtgcagcctg gagaagaacg agagggcttg catcgacttt
     6181 gccatcagcg ccagacccct gacgcggtac atgccccaga acaagcagtc gttccagtat
     6241 aagacgtgga cgttcgtggt gtccccacct tttgagtact tcatcatggc tatgatcgcc
     6301 ctcaacactg tggtgctgat gatgaagttc tacgatgcgc cgtacgagta tgagctaatg
     6361 ctgaaatgcc tgaacatcgt gttcacgtcc atgttctcca tggagtgtgt gctgaagatc
     6421 atcgccttcg gggtgctgaa ctatttcaga gatgcctgga atgtctttga ctttgtcacg
     6481 gtgttgggaa gtattactga tattttggta acagagattg cgaacaactt catcaacctc
     6541 agcttcctgc gcctgttccg ggccgcgcgg ctcatcaagc tgctgcgcca gggctacacc
     6601 atccgcatcc tgctttggac cttcgtgcag tccttcaagg cgctgcccta cgtgtgtctc
     6661 ctcatcgcca tgctcttctt catctacgcc atcattggca tgcaggtttt tgggaacatc
     6721 gccctggacg atgacaccag catcaacagg cacaataact tccggacgtt tctgcaggcc
     6781 ttgatgttgc tgttcaggag cgccacgggg aaggcctggc acgagataat gctgtcgtgc
     6841 ctcagcagcc gggcctgcga cgagcactcc aatgccagcg agtgcggcag cgactttgcc
     6901 tacttttact tcgtctcctt catcttcctc tgctcctttc tgatgttgaa cctctttgtg
     6961 gctgtgatca tggacaattt tgagtacctt acacgggact cttccatctt agggcctcac
     7021 cacctggatg agttcatccg ggtctgggct gaatacgacc cggccgcgtg tgggcgcatc
     7081 agttacagtg acatgtttga gatgctgaag cacatgtccc cacccctggg gctggggaag
     7141 aagtgccctg ctcgagtcgc gtacaagcgc ctggtccgca tgaacatgcc catctccagt
     7201 gaggacatga cggtgcactt cacatccacg ctgatggctc tcattcggac agcgctggac
     7261 atcaagctgg caccagctgg gaccaagcag caccagtgcg acgcagagct gaggaaggag
     7321 atttcctgtg tgtgggccaa tctgccccag aagaccctgg acttactggt accaccccat
     7381 aagcctgacg agatgacggt gggcaaagtc tatgcagctc tgatgatctt cgacttctac
     7441 aaacagaaca aaaccagcag agaccagact cagcaagccc ccggaggcct gtcccagctg
     7501 ggccccgtgt ccctgttcca cccgctgaag gccacgctgg agcagactca gccggcgctg
     7561 cgtggagccc gggctttcct gcggcagaag agttctgcct ccctgagcaa cggcggggcc
     7621 gtacaaaccc aggagagcgg catcaaggaa tcggtttctt ggggcactca gaggacccag
     7681 gatgtgcttt gcgaggccag ggcacccctg gagcggggcc actcagctga gatcccagtc
     7741 gggcagcctg ggacactggc tgtagatgtc cagatgcaga acatgactct gagtggccct
     7801 gacgccgagc cgcagccagg gctggagagc cagggccgag ccgcctccat gccccgcctg
     7861 gccgcagaaa cacagcccgc gcctgacgcc agccccatga agcgctccat ctccacgctg
     7921 gccccgcgcc cccacacggc ccggctcggc agcaccgcgc tggaccgccc agcccccagc
     7981 caggccccgc accatcacca ccaccgctgc caccgccgca gggacaggaa gcagcggtcc
     8041 ctggagaagg gtcccagcct gtctgctgac acagatgggg cccccgacag cacggtgggg
     8101 ccaggactgc ccacggggga gggccccccc ggctgcaggc gggagcggga gcgcaggcag
     8161 gagcggggcc ggtcccagga gcggaggcag ccctcgtcct cctcctcgga gaagcatcgc
     8221 ttctactcct gtgaccgctt cgggggccgc gaacccccac agcccaagcc ctccctcagt
     8281 agtcacccca cgtcgccgac ggccgggcag gagccgggac cccacccgca ggggagtggt
     8341 tcggttcacg ggagccccct gctgtccaca tctggtgcta gcaccccggg ccgggggcgg
     8401 aggcagctcc cccagacccc cttgaccccc cgccccagcg tcacctacaa gacggccaac
     8461 tcctcgcctg tccacttcgc cggggcaccg agcggcctcc ccgccttctc ccccggccgg
     8521 ctcagccggg gcctctcaga acacaacgcc ctgctgcaga gggacccgct cagccggccc
     8581 ctggcgcccg gctcgcgcat cggctccgac ccctacctgg ggcagcggct ggacagcgag
     8641 gcgcccgccc gtgccctgcc cgaggacgcg cccgcctttg aggagaccgc ggccagcaac
     8701 tcgggccgct cctcccggac ctcgtacgtg tcctcactga cctcgcagcc cccgccgctg
     8761 cgccgcgtgc ccaacggcta ccactgcact ctggggctgg gcgggggcgg ccgggcgcgg
     8821 cgcggctgcc accaccccga ccgcgaccgc cggtgctaga ctagttctag caagcttcga
     8881 cgaattcgca tgcggccgct agctcgaggt accgatatcg agctcaattc actcctcagg
     8941 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     9001 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     9061 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     9121 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     9181 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     9241 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     9301 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     9361 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     9421 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     9481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     9661 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9781 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9841 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     9901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9961 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
    10021 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    10081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10981 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11041 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11101 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11161 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11221 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11281 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11341 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11401 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11461 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11521 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11581 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11641 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11701 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11761 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11821 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
