LOCUS       sequence-406758-       12600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and a mutation (W391A) in the ?1-interacting 
            domain (AID) in the I-II loop of Cav2.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Waithe D, Ferron L, Page KM, Chaggar K, Dolphin AC
  TITLE     Beta-subunits promote the expression of Ca(V)2.2 channels by 
            reducing their proteasomal degradation.
  JOURNAL   J Biol Chem. 2011 Mar 18;286(11):9598-611. doi: 
            10.1074/jbc.M110.195909. Epub 2011 Jan 13.
  PUBMED    21233207
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.195909"; journalName: "J Biol Chem"; date: 
            "2011-03-18- 18"; volume: "286"; issue: "11"; pages: "9598-611"
COMMENT     Sequence Label: Cav2.2 HA W391A pcDNA3
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(474..492)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(475..492)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(512..529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    518..742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      788..1216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(875..894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1085..1106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1225..1245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1230..1559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1410..1545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1472..1491
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1626..2420
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1680..1699)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2290..2309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2594..2715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2631..2650)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2685..2704
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2764..2780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2777..2799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2788..2804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2812..2818,2819..2836,2837..2842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2857..2878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2995..3012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3166..3754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3246..3265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3925..4716,4717..4785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4548..4567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4786..4890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4965..4984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5156..5535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5536..5739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5690..5710
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5784..5803
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5784..5802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7604..7630
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7634..7660
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgtccacttc gccggggcac cgagcggcct ccccgccttc tcccccggcc ggctcagccg
       61 gggcctctca gaacacaacg ccctgctgca gagggacccg ctcagccggc ccctggcgcc
      121 cggctcgcgc atcggctccg acccctacct ggggcagcgg ctggacagcg aggcgcccgc
      181 ccgtgccctg cccgaggacg cgcccgcctt tgaggagacc gcggccagca actcgggccg
      241 ctcctcccgg acctcgtacg tgtcctcact gacctcgcag cccccgccgc tgcgccgcgt
      301 gcccaacggc taccactgca ctctggggct gggcgggggc ggccgggcgc ggcgcggctg
      361 ccaccacccc gaccgcgacc gccggtgcta gactagttct agcaagcttc gacgaattct
      421 gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc tattctatag
      481 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
      541 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      601 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      661 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      721 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      781 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1081 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1141 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1201 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     1261 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     1321 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1381 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1441 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     1501 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     1561 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     1621 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     1681 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     1741 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     1801 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1861 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1921 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     1981 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     2041 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     2101 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     2161 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2221 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2281 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2341 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     2401 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     2461 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2521 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     2581 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2641 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2701 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     2761 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2821 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2881 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     4921 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     4981 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     5041 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     5101 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     5161 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     5221 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5281 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     5341 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     5401 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     5461 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     5521 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     5581 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     5641 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     5701 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     5761 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
     5821 cgagctcgga tccactagta acggccgcca gtgtgctgga attcctcgag ggtaccgggc
     5881 cccccctcga ggtcgaagct tgatcatggt ccgcttcggg gacgagctgg gcggccgcta
     5941 tgggggcgcg ggcggcgcgg agcgggcccg gggcggcggg gccggcgggg ctggcggccc
     6001 gggccccggg gggctgccgc cgggccagcg ggtcctctac aaacagtcga tcgcgcagcg
     6061 cgcgcggacc atggcgctgt acaaccccat cccggtcaag cagaactgct tcaccgtcaa
     6121 ccgctcgctc ttcgtcttca gcgaggacaa cgtcgtccgc aaatacgcca agcgcatcac
     6181 cgagtggcct ccgttcgagt acatgatcct ggccaccatc atcgccaact gcatcgtgct
     6241 ggccctggag cagcacctcc ctgacgggga caagacgcct atgtccgagc gcctggatga
     6301 caccgagccc tacttcattg ggatcttctg cttcgaggcg gggatcaaga tcctggccct
     6361 gggctttgtt ctgcacaagg gctcctatct gcggaacggc tggaacgtca tggacttcgt
     6421 ggtagtcctc acggggattc ttgccacagc cggaaccgac ttcgacctgc gaacgctgag
     6481 ggctgtgcgt gtgctaaggc ccctgaagct ggtgtctggg attccgagct tgcaggtggt
     6541 gctcaagtcc atcatgaagg ccatggtccc gctgctgcag atcgggctgc tgctcttctt
     6601 cgccatcctc atgtttgcca tcatcggcct cgagttctac atgggcaagt tccacaaggc
     6661 ctgcttcccc aacagcacag acccagatcc cgtgggtgac ttcccctgtg gcaaggaggc
     6721 cccggcccgg ctctgtgagg gagacacgga gtgccgggag tactgggccg gacccaactt
     6781 tggtatcacc aactttgaca acatcctgtt tgccatcttg accgtgttcc agtgcatcac
     6841 catggagggc tggactgaca tcctctacaa tacaaacgat gcagccggca acacgtggaa
     6901 ctggctctac ttcatccctc tcatcatcat cggctccttc ttcatgctca acctggtgct
     6961 gggtgtgctc tcaggagagt ttgctaagga gcgcgagaga gtggagaacc gccgcgcctt
     7021 cctgaagctc cggcggcagc agcagattga gcgggagctc aacgggtacc tggaggcgat
     7081 cttcaaggct gaggaagtca tgctggctga ggaggacagg aacgcagagg agaagtcccc
     7141 tctggatgca gtgttgaaga gagcagctgc caagaagagc agaagcgacc tgatccaggc
     7201 cgaggagggg gagggccggc tcacaggcct ctgtgctccc ggttccccgt tcgcccgtgc
     7261 cagcctgaaa agcgggaaga cggagagctc gtcgtacttc cggaggaagg agaagatgtt
     7321 ccgcttcttc atccggcgca tggtgaaggc acagagcttc tactggacgg tgctgtgtgt
     7381 ggtggcactg aacacactgt gcgtggccat ggtgcactac aaccagccgc agcggctcac
     7441 cacggcattg tactttgcag agtttgtttt cctgggtctc ttcctcacag agatgtccct
     7501 gaagatgtat ggcctggggc ccaggagcta cttccggtcc tccttcaact gttttgactt
     7561 tggggtcatc gtgggcagta tctttgaagt ggtctgggct gcttacccct acgacgtccc
     7621 cgactacgcc ggctacccgt acgacgtccc ggactacgcc ggcgtcgacg tcaagccagg
     7681 gacctccttc gggatcagtg ttctgcgggc tctgcgctta ctgagaatct tcaaggtcac
     7741 caaatactgg aactccctac ggaacctggt ggtgtctctg ctcaactcca tgaagtccat
     7801 catcagcctc ctcttcctgc tcttcctgtt cattgttgtc tttgctctgc tggggatgca
     7861 gctgtttgga ggccagttca actttaaaga cgagacccca acgaccaact ttgacacctt
     7921 ccctgctgct atcctcaccg tcttccagat cctgaccgga gaggactgga atgcggtcat
     7981 gtaccacggg atcgagtcgc aaggtggcgt cagcagagga atgttctcct ccttctactt
     8041 cattgtcctc actctgtttg ggaactacac cctgctgaat gtcttcctgg ccattgctgt
     8101 ggacaacctt gccaatgctc aggagctgac caaggatgaa gaggagatgg aagaagcagc
     8161 caatcagaaa ctcgccttgc agaaggccaa ggaagtggct gaagtcagcc ccatgtctgc
     8221 tgcgaacatc tccattgccg ccaggcagca gaactcggcc aaagcgcgct cggtgtggga
     8281 gcagcgagcc agccagctgc ggctgcagaa cctgagggcc agctgcgagg cgctgtacag
     8341 cgagatggac cccgaggaga ggctgcgcta cgccaccgcg cgccacctgc ggcccgacgt
     8401 gaagacgcac ctggaccggc cgctggtggt tgagcccggc cgcgacgctc cgcggggccc
     8461 cccggggggg aagtcccggc cggatggctc ggaggccccc gagggtgcgg atcccccgcg
     8521 caggcatcac cggcaccgag acaaggacaa ggctcccgcc acggtcccct cggcggggga
     8581 acaggacaga gccgaagctc tgagggcaga gggcggggag ctggggcccc gcgaggagcg
     8641 ggggcggcca cgccgcagcc gcagcaagga ggccccgggc gccccggagg tgcgcagcga
     8701 tcgcggccgt ggcccgtgtc ctgaaggtgg tcggcgccac caccggcgcg ggtcaccgga
     8761 ggaggcagct gagagggagc cccgccgcca ccgcgcgcac cggcacgggc ccgacccggg
     8821 caaggagggc ccagcgtccg ggaccagggg cgagcggcgc gcacgacacc gcacgggccc
     8881 ccgggcgtgc ccccgggagg ctgagagcag cgaggagcct gcgcggcggc accgggctcg
     8941 gcacaaggcg ccgcccaccc aggagaccgc ggagaaggac aaggaggctg ccgagaaggg
     9001 gggcgaggcc acggaggccg agaaggacaa ggaggctcgg aaccaccagc ccaaggagct
     9061 gccctgtgac ctggaggcca tcggcatgct gggagtgggc gctgtgcata cgctgcccag
     9121 cacctgtctg cagaaggtgg aggagcagcc agaggatgcg gacaaccagc ggaacgtcac
     9181 ccgcatgggt agtcagcccc cggacaccag caccactgtg cacattcctg tgacgctgac
     9241 cggccccccc ggggagacca cggtagtgcc cagtggaaac gtggacctgg aaagtcaagc
     9301 agaggggaag aaggaggtgg aaaccagcga tgtgatgagg agcggccctc ggcccattgt
     9361 cccgtacagt tccatgttct gtttaagccc aaccaacctg ctgcgccgct gctgccacta
     9421 cattgtgacc atgcgatact tcgagatggt catcctcgtg gtcatcgccc tgagcagcat
     9481 cgccctggct gccgaggacc ccgtgagaac agactctccc aggaataatg ctctgaagta
     9541 catggactac atcttcacag gcgtcttcac cttcgagatg gtgataaaga tgatcgattt
     9601 gggactgctg ctacaccctg gagcctattt ccgggatctg tggaacattc tggacttcat
     9661 tgtggtcagt ggggccctgg tggcatttgc tttctcagga tccaaaggga aagacatcag
     9721 caccatcaaa tccctgagag tcctgcgtgt gctgcggcca ctcaagacca tcaagcggct
     9781 gcccaagctc aaggccgtgt ttgactgtgt ggtgaactca ctgaaaaacg tcctcaacat
     9841 cctgatcgtc tacatgctct tcatgttcat ctttgccgtc attgccgtgc agctcttcaa
     9901 agggaagttc ttctactgca cggacgaatc caaggagctg gagcgtgact gcaggggtca
     9961 gtatttggat tatgagaagg aggaagtgga agctcaaccg aggcagtgga agaaatatga
    10021 cttccactat gacaacgtgc tctgggctct gctaacgctc ttcacggtgt ccacagggga
    10081 agggtggccc atggtgctga aacactctgt ggatgccacc tacgaggagc agggcccgag
    10141 ccccgggtac cgcatggagc tctccatctt ctacgtggtc tactttgtgg tctttccctt
    10201 cttctttgtc aacatcttcg tggccttgat catcatcacc ttccaggagc agggggacaa
    10261 ggtgatgtcc gagtgcagcc tggagaagaa cgagagggct tgcatcgact ttgccatcag
    10321 cgccagaccc ctgacgcggt acatgcccca gaacaagcag tcgttccagt ataagacgtg
    10381 gacgttcgtg gtgtccccac cttttgagta cttcatcatg gctatgatcg ccctcaacac
    10441 tgtggtgctg atgatgaagt tctacgatgc gccgtacgag tatgagctaa tgctgaaatg
    10501 cctgaacatc gtgttcacgt ccatgttctc catggagtgt gtgctgaaga tcatcgcctt
    10561 cggggtgctg aactatttca gagatgcctg gaatgtcttt gactttgtca cggtgttggg
    10621 aagtattact gatattttgg taacagagat tgcgaacaac ttcatcaacc tcagcttcct
    10681 gcgcctgttc cgggccgcgc ggctcatcaa gctgctgcgc cagggctaca ccatccgcat
    10741 cctgctttgg accttcgtgc agtccttcaa ggcgctgccc tacgtgtgtc tcctcatcgc
    10801 catgctcttc ttcatctacg ccatcattgg catgcaggtt tttgggaaca tcgccctgga
    10861 cgatgacacc agcatcaaca ggcacaataa cttccggacg tttctgcagg ccttgatgtt
    10921 gctgttcagg agcgccacgg gggaggcctg gcacgagata atgctgtcgt gcctcagcag
    10981 ccgggcctgc gacgagcact ccaatgccag cgagtgcggc agcgactttg cctactttta
    11041 cttcgtctcc ttcatcttcc tctgctcctt tctgatgttg aacctctttg tggctgtgat
    11101 catggacaat tttgagtacc ttacacggga ctcttccatc ttagggcctc accacctgga
    11161 tgagttcatc cgggtctggg ctgaatacga cccggccgcg tgtgggcgca tcagttacag
    11221 tgacatgttt gagatgctga agcacatgtc cccacccctg gggctgggga agaagtgccc
    11281 tgctcgagtc gcgtacaagc gcctggtccg catgaacatg cccatctcca gtgaggacat
    11341 gacggtgcac ttcacatcca cgctgatggc tctcattcgg acagcgctgg acatcaagct
    11401 ggcaccagct gggaccaagc agcaccagtg cgacgcagag ctgaggaagg agatttcctg
    11461 tgtgtgggcc aatctgcccc agaagaccct ggacttactg gtaccacccc ataagcctga
    11521 cgagatgacg gtgggcaaag tctatgcagc tctgatgatc ttcgacttct acaaacagaa
    11581 caaaaccagc agagaccaga ctcagcaagc ccccggaggc ctgtcccagc tgggccccgt
    11641 gtccctgttc cacccgctga aggccacgct ggagcagact cagccggcgc tgcgtggagc
    11701 ccgggctttc ctgcggcaga agagttctgc ctccctgagc aacggcgggg ccgtacaaac
    11761 ccaggagagc ggcatcaagg aatcggtttc ttggggcact cagaggaccc aggatgtgct
    11821 ttgcgaggcc agggcacccc tggagcgggg ccactcagct gagatcccag tcgggcagcc
    11881 tgggacactg gctgtagatg tccagatgca gaacatgact ctgagtggcc ctgacgccga
    11941 gccgcagcca gggctggaga gccagggccg agccgcctcc atgccccgcc tggccgcaga
    12001 aacacagccc gcgcctgacg ccagccccat gaagcgctcc atctccacgc tggccccgcg
    12061 cccccacacg gcccggctcg gcagcaccgc gctggaccgc ccagccccca gccaggcccc
    12121 gcaccatcac caccaccgct gccaccgccg cagggacagg aagcagcggt ccctggagaa
    12181 gggtcccagc ctgtctgctg acacagatgg ggcccccgac agcacggtgg ggccaggact
    12241 gcccacgggg gagggccccc ccggctgcag gcgggagcgg gagcgcaggc aggagcgggg
    12301 ccggtcccag gagcggaggc agccctcgtc ctcctcctcg gagaagcatc gcttctactc
    12361 ctgtgaccgc ttcgggggcc gcgaaccccc acagcccaag ccctccctca gtagtcaccc
    12421 cacgtcgccg acggccgggc aggagccggg accccacccg caggggagtg gttcggttca
    12481 cgggagcccc ctgctgtcca catctggtgc tagcaccccg ggccgggggc ggaggcagct
    12541 cccccagacc cccttgaccc cccgccccag cgtcacctac aagacggcca actcctcgcc
//
