LOCUS       sequence-406759-       12359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 calcium channel with a E1686R mutation to 
            enhance expression and an exofacial double HA tag in domain II .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin 
            AC
  TITLE     Dominant-negative synthesis suppression of voltage-gated calcium 
            channel Cav2.2 induced by truncated constructs.
  JOURNAL   J Neurosci. 2001 Nov 1;21(21):8495-504.
  PUBMED    11606638
REFERENCE   2  (bases 1 to 12359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2001-11-1"; volume: "21(21)"; pages: "8495-504"
COMMENT     Sequence Label: Cav2.1 HA pCAGGS
FEATURES             Location/Qualifiers
     source          1..12359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          613..1631
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1553..1575
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1639..1658
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3441..3467
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3471..3497
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8274..8303
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(9380..9399)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9445..9500
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9499..9518)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9861..9877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9861..9877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9874..9896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9885..9901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9909..9915,9916..9933,9934..9939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9954..9975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10034..10229
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10080..10215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10235..10369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10272..10291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10326..10345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10437..10454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10608..11196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10688..10707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11367..12158,12159..12227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11990..12009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12228..12332)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggs gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      541 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      601 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      661 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      721 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      781 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
      841 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
      901 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
      961 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     1021 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     1081 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     1141 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     1201 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     1261 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     1321 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     1381 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     1441 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1501 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1561 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc attgttcatt gccttcttct
     1621 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     1681 aattcgcatt gcggccgctt agaccggtct cgaggtcttg cagaatggcc cgctttggag
     1741 acgagatgcc gggccgctac ggcgcaggcg gaggaggctc agggccggcc gccggggtgg
     1801 tcgtgggcgc cgcgggcggc cgaggagccg ggggcagccg gcagggcggg cagcccggag
     1861 cgcagaggat gtacaagcag tcgatggcgc agagagcgcg gaccatggcc ctctacaacc
     1921 ccatccctgt ccgccagaac tgcctcacgg tcaaccgctc cctgttcctc ttcagtgaag
     1981 acaacgtggt gagaaaatac gccaaaaaga tcacggaatg gcctcccttc gagtacatga
     2041 tcctggccac catcattgct aactgcatcg tcctggccct ggagcagcac ctccctgatg
     2101 atgacaagac acccatgtcc gagcggctgg atgacacaga accctatttc attggcatct
     2161 tctgttttga ggctggaatt aagatcgtgg ctcttggctt tgccttccac aaaggctcct
     2221 acctgaggaa tggctggaac gtcatggact ttgtcgtggt gctaacaggc atcttggcca
     2281 ctgtcgggac ggagtttgat ctacggacac tgagggcggt tcgtgtgctg cggccactca
     2341 agctggtgtc tggaatccca agtttacaag tcgtcctgaa gtcaatcatg aaggcgatga
     2401 tccctctgct gcagatcggc ctcctcctgt tttttgcaat ccttattttt gcaatcatag
     2461 ggttagaatt ttatatggga aaatttcata ccacctgctt tgaagagggg acagacgaca
     2521 tccagggtga gtcgccagct ccgtgtggga cagaggagcc tgcccgcacc tgccccaacg
     2581 ggaccaaatg tcagccgtac tgggaagggc ccaacaacgg catcactcag ttcgacaaca
     2641 tcctgtttgc tgtgctcact gttttccagt gcatcaccat ggaaggctgg actgatctcc
     2701 tctacaatag caacgatgcc tcagggaaca cttggaactg gttgtacttc atccccctca
     2761 tcatcatcgg ctcctttttt atgctgaacc ttgtgctggg tgtgctgtct ggggagtttg
     2821 ccaaagaaag ggaacgtgta gagaaccgaa gggcttttct gaagctcaga agacaacagc
     2881 agattgaacg tgagctcaat ggatacatgg agtggatctc gaaagcagaa gaggtgatcc
     2941 tcgcggagga cgaaacagac gtggagcaga ggcacccttt tgatggagct cttcggagag
     3001 ctactctgaa gaaaagcaag acggacctgc tcaaccctga ggaggcggag gaccagcttg
     3061 ctgacatcgc gtctgtgggg tctcccttcg ccagagccag catcaaaagt gccaagctgg
     3121 agaattcgac ttttttccac aaaaaggaga gaagaatgcg tttctacatc cgccgcatgg
     3181 tcaaaactca ggccttctac tggaccgtgc tcagtctggt agccctcaac acgctgtgcg
     3241 tcgccattgt ccactacaac cagcccgagt ggctctccga cttcctctac tatgcagaat
     3301 tcattttctt aggactcttt atgtccgaaa tgtttataaa aatgtatggg ctcgggacac
     3361 ggccttactt ccactcttcc ttcaactgct ttgactgtgg ggtcatcatc gggagcatct
     3421 ttgaagtcat ctgggccgtc tacccctacg acgtccccga ctacgccggc tacccgtacg
     3481 acgtcccgga ctacgccggc gtcgacatca aaccgggtac atcctttgga atcagcgtgt
     3541 tacgagctct caggttactg cgtattttca aagtcacaaa gtactgggca tctctcagaa
     3601 acctggttgt ctccctcctc aactccatga aatccatcat aagtctgctg ttcctcctct
     3661 tcctcttcat tgtcgtcttt gccctcttgg ggatgcagct gtttggtggc cagtttaatt
     3721 ttgacgaggg gactcctccc accaacttcg acacttttcc agcagcaata atgactgtgt
     3781 ttcagatcct gactggcgag gattggaatg aggtcatgta tgatgggatc aagtctcagg
     3841 ggggcgtgca gggcggcatg gtgttctcca tctacttcat cgtcctcacc ctcttcggga
     3901 actacaccct gctgaacgtg ttcttggcta tcgcggtgga caacctggcc aacgcccagg
     3961 aactcaccaa ggatgaacaa gaagaggaag aggcagccaa tcagaaactg gctctacaga
     4021 aagccaagga ggtggcagaa gtgagtcccc tgtctgcagc caacatgtcc atagctgtga
     4081 aggaacagca gaagaaccag aagcctgcca agtcggtgtg ggagcagcgc accagcgaga
     4141 tgcgcaagca gaacctgctg gctagccgcg aggcgctgta cggggacgcg gctgagcgct
     4201 ggcccaccac ttacgcgcgc ccgctgcggc cggacgtgaa gacgcacttg gaccggccgc
     4261 tcgtggtgga cccgcaggag aaccgtaaca acaacaccaa caagagccgt gcgccagaag
     4321 cgctgcgcca aaccgcgcgg ccccgcgaga gcgcgcgcga ccccgacgcg cggcgcgcct
     4381 ggcccagcag ccctgagcgc gcccctggac gagagggccc gtatggccgc gagagcgagc
     4441 cgcaacagcg cgagcacgcg ccaccccgcg agcacgtacc ctgggacgcg gatcctgagc
     4501 gcgccaaggc cggggacgcg ccccgccgcc acacgcaccg gcctgtggcc gagggcgagc
     4561 ctcgtcgcca ccgcgcgcgc cgccggcccg gggacgaacc ggacgacaga ccggagcgca
     4621 ggccgcgtcc ccgcgacgcc actaggccgg cccgcgctgc agacggcgaa ggcgatgatg
     4681 gggagcgcaa gcggcgacac cgacacgggc cgccggccca cgatgacagg gagcgcagac
     4741 accggcgcag aaaagagagc cagggctctg gggtccccat gtctggtccc aacctgtcca
     4801 ccaccaggcc aatccagcag gatctgggcc gccaggacct gccactggct gaggacctgg
     4861 acaacatgaa gaacaacaag ttggccaccg gggagcctgc cagtccccac gacagcctgg
     4921 gccacagtgg ccttccccct agccctgcca agatcgggaa cagcaccaac cctggtcccg
     4981 ccttggccac caatccccag aatgctgcca gccgcaggac gcccaacaac ccgggcaacc
     5041 cgtccaaccc cggccccccc aagactcccg agaacagcct tatcgtcacc aaccccagca
     5101 gcacccagcc caactcagca aagactgcca ggaaacccga gcacatggcg gtggagatcc
     5161 ccccggcctg cccgcccctc aaccacactg tggtccaagt aaacaaaaac gccaacccag
     5221 acccactgcc aaagaaagag gaagagaaga aggaggaaga ggaggcagac cccggggagg
     5281 atggcccaaa gcccatgccg ccctacagct ccatgttcat cctctccacc accaaccccc
     5341 ttcgccggct gtgccattac atcctgaacc tgcgctactt cgagatgtgc atcctcatgg
     5401 tcattgccat gagtagcatc gcgctggccg ccgaggaccc ggtgcagccc aacgcacccc
     5461 gcaacaacgt gctgcgatat tttgactatg ttttcacagg agtgtttacc tttgagatgg
     5521 tgatcaagat gatcgacctg ggcctcgtcc tgcatcaggg ggcctatttc cgtgacctgt
     5581 ggaacattct ggacttcata gtggtcagtg gggccctggt ggcctttgcc ttcactggca
     5641 atagcaaagg aaaggacatc aacaccatca agtccctccg agtcctccgg gtgctacgac
     5701 ctctaaagac catcaagcgg ctgcctaagt tgaaggccgt atttgactgc gtggtgaact
     5761 cgctcaagaa cgtcttcaac atcctcattg tctacatgct cttcatgttc atcttcgccg
     5821 tggtggccgt gcagctcttc aagggcaaat tcttccactg cacggacgag tccaaggagt
     5881 ttgagagaga ctgtcgaggc aaatacctcc tttacgagaa gaacgaggta aaggcgcggg
     5941 accgcgagtg gaagaaatac gacttccact acgacaacgt gctctgggcc ctgctcacgc
     6001 tctttacggt gtccacggga gagggctggc cacaggtcct caagcactca gtggatgcca
     6061 cttttgagaa ccagggcccc agccccgggt accgcatgga aatgtccatc ttctacgtgg
     6121 tctactttgt ggtgtttccc ttcttctttg tcaatatctt tgtggccttg atcatcatca
     6181 ccttccagga gcagggagac aagatgatgg aagaatacag cctagagaaa aatgagaggg
     6241 cctgcatcga ctttgccatc agtgccaagc cgctgaccag gcacatgccc cagaacaagc
     6301 agagcttcca gtatcgaatg tggcagttcg tggtgtcccc accctttgag tacaccatca
     6361 tggccatgat cgctctcaac accatcgtgc taatgatgaa gttctatgga gcctctgtgg
     6421 cctatgaaaa cgcccttcga gtgttcaaca ttgtcttcac ctccctcttc tctctcgaat
     6481 gtgtgctcaa agtcatggct tttgggattc tgaattattt ccgcgatgcc tggaacatct
     6541 tcgactttgt gactgttctg ggcagcatca cagacatcct cgtcaccgag tttgggaata
     6601 acttcatcaa cctgagcttt ctccgcctct tccgtgctgc ccgactcatc aaactcctcc
     6661 gccagggtta caccatccgc attctcctct ggactttcgt gcagtctttc aaggccctac
     6721 cttatgtctg tctgctgatc gccatgctct tcttcatcta tgccatcatc gggatgcagg
     6781 tgtttggcaa catcggcatt gatggggaag atgaggacag cgatgaggat gagttccaaa
     6841 tcacgaggca caataacttc cggaccttct tccaagctct catgcttctc ttccggagcg
     6901 ccacagggga agcgtggcac aacatcatgc tgtcctgcct cagcgggaag ccatgcgaca
     6961 agaactccgg gatccaaaaa ccagagtgtg gcaacgagtt cgcctatttt tactttgtct
     7021 cgttcatctt cctttgctca tttctgatgc tgaatctctt tgttgctgtc atcatggaca
     7081 acttcgagta cctcacccga gattcctcca tcctgggccc ccaccacctg gatgagtacg
     7141 tgcgtgtctg ggcagagtat gaccctgctg cctgcggccg cattcactat aaggacatgt
     7201 acagtttatt gcgagtaata tcgccccctc tcggcttagg caagaaatgt cctcataggg
     7261 ttgcttgcaa gaggctcttg cggatggacc tacccgtagc ggatgacaac accgttcact
     7321 tcaactccac cttgatggct ctgatccgaa ccgccctgga tatcaaaatc gcaaagggtg
     7381 gagctgacaa gcagcaaatg gacgcagagc tccgcaagga aatgatggcc atttggccca
     7441 acctgtctca gaagaccttg gatctgctgg tcacacctca caagtccacg gacctgacag
     7501 tgggtaagat ctacgcagcc atgatgatca tggagtacta ccggcagagc aaggccaaga
     7561 agctgcaggc catgcgagag gagcagaacc ggacaccact catgttccag cgcatggagc
     7621 ctccatcgcc aacacaggag ggaggaccca gccaaaacgc ccttccctcc actcagctgg
     7681 accccggagg aggcctgatg gctcaagaaa gcagcatgaa ggagagcccg tcctgggtga
     7741 cccagcgggc acaggagatg ttccagaaga ctggtacctg gagcccagag cgagggccac
     7801 ccatcgacat gcctaacagc cagcccaact cccagtctgt ggagatgaga gaaatgggaa
     7861 ctgatggcta ctcagacagc gaacactacc tccccatgga aggacagacc agggccgcct
     7921 ccatgccccg cctcccagca gagaaccaga ggagaagggg ccggccacgt ggaaataacc
     7981 tcagtaccat ctctgatacc agccccatga agcgctcagc ctccgtgctg ggacccaaag
     8041 cccggcgact ggatgactac tcactagagc gggtaccacc tgaggagaac caaaggtacc
     8101 accaacgccg ccgggaccgt ggccaccgca cctctgagcg ctctctgggc cgatacactg
     8161 atgtggacac aggcctgggg acagatctga gcatgaccac ccaatcgggt gacctgccct
     8221 ccaaagatcg ggaccaggac cggggccggc ccaaggaccg gaagcatcgg ccacaccacc
     8281 accaccacca tcatcaccat catcccccgg ccccggaccg ggagcgctac gcacaggagc
     8341 ggccggacac cggccgggcg cgggcccggg agcagcgctg gtcccgctcg cccagcgagg
     8401 gtcgggagca cgcgacacac agacagtagt tccgtcagtg gaagcccagc cccctctacg
     8461 tccggcacca gcacgccgcg gcgcggccgc cgccagctcc cccagacccc ctgcaccccg
     8521 cggccgcttg tgtcctactc gcccgctccg cgcaggcctg cggcgcgcag gatggcgggt
     8581 cccccagcgc cccctggcgg ctcgccgcgg ggttgccgcc gtgcgccgcg ctggcctgcg
     8641 catgcgcctg agggcccgcg gccgcgcggt gccgactaca ccgagcccga cagcccgcgc
     8701 gagccgccag ggggcgcgca tgagcccgcg ccgcggtcgc ccaggactcc gcgcgccgcg
     8761 ggctgcgcgt caccgcgcca cggccgcagg ctgcccaatg gttactacgc ggggcacggc
     8821 gcgccacggc cgcgcacagc ccgcaggggc gcgcacgacg cgtacagcga gagcgaggac
     8881 gactggtgct aagccgcccc gccccccgca gcgcccgtac ggtgcgggcg caccgcaaat
     8941 gataaacaat aacgttttta aaaaagaaaa ccggggagaa aaacaaaaat agcttctatt
     9001 gatgagtttt atcatctcga ttgaatcttt cgttttcccg gtgaacgaaa ccgaggtggc
     9061 tgcgagccgg agggacggct gcacgcccca cacacttctc agacccacgg gagttcttgc
     9121 gggccacgcc aaacttatta ctgcctgcag agttcaacta caagaaaata acactaacag
     9181 tttttaaaag gactccgtaa aaagaaattt gaaagagaat gtagagaggc tgagaaagcc
     9241 caggaattcc tgcagcccgg gggatccact agtctcgagg aattcggtac cgagctcaat
     9301 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     9361 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     9421 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     9481 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     9541 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     9601 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     9661 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     9721 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     9781 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     9841 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9901 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9961 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10021 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    10081 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10141 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    10201 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
    10261 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10321 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
    10381 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10501 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10561 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10621 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10681 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10741 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10801 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10861 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10921 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10981 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11041 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11101 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11161 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11221 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11281 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11341 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12241 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12301 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgg
//
