LOCUS       sequence-406760-       12534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, 
            this enhances the expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12534)
  AUTHORS   Page KM, Heblich F, Davies A, Butcher AJ, Leroy J, Bertaso F, Pratt 
            WS, Dolphin AC
  TITLE     Dominant-negative calcium channel suppression by truncated 
            constructs involves a kinase implicated in the unfolded protein 
            response.
  JOURNAL   J Neurosci. 2004 Jun 9;24(23):5400-9. doi: 
            10.1523/JNEUROSCI.0553-04.2004.
  PUBMED    15190113
REFERENCE   2  (bases 1 to 12534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0553-04"; journalName: "J Neurosci"; date: 
            "2004-06-9- 9"; volume: "24"; issue: "23"; pages: "5400-9"
COMMENT     Sequence Label: Cav2.2 pcDNA3
FEATURES             Location/Qualifiers
     source          1..12534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6874..6893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6875..6893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6938..7141)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6967..6987)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7142..7521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7693..7712
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7787..7891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7892..7960,7961..8752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8110..8129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8923..9511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9412..9431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9665..9682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9799..9820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9835..9840,9841..9858,9859..9865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9873..9889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9878..9900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9897..9913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9897..9913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9962..10083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9973..9992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10027..10046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10257..11051)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10368..10387)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10978..10997
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(11118..11447)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11132..11267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11186..11205)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     11432..11452
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(11461..11889)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11571..11592)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11783..11802
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    11935..12159
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12148..12165
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        12185..12203
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     12185..12202
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtcggagccg atgcgcgagc cgggcgccag gggccggctg agcgggtccc tctgcagcag
       61 ggcgttgtgt tctgagaggc cccggctgag ccggccgggg gagaaggcgg ggaggccgct
      121 cggtgccccg gcgaagtgga caggcgagga gttggccgtc ttgtaggtga cgctggggcg
      181 gggggtcaag ggggtctggg ggagctgcct ccgcccccgg cccggggtgc tagcaccaga
      241 tgtggacagc agggggctcc cgtgaaccga accactcccc tgcgggtggg gtcccggctc
      301 ctgcccggcc gtcggcgacg tggggtgact actgagggag ggcttgggct gtgggggttc
      361 gcggcccccg aagcggtcac aggagtagaa gcgatgcttc tccgaggagg aggacgaggg
      421 ctgcctccgc tcctgggacc ggccccgctc ctgcctgcgc tcccgctccc gcctgcagcc
      481 gggggggccc tcccccgtgg gcagtcctgg ccccaccgtg ctgtcggggg ccccatctgt
      541 gtcagcagac aggctgggac ccttctccag ggaccgctgc ttcctgtccc tgcggcggtg
      601 gcagcggtgg tggtgatggt gcggggcctg gctgggggct gggcggtcca gcgcggtgct
      661 gccgagccgg gccgtgtggg ggcgcggggc cagcgtggag atggagcgct tcatggggct
      721 ggcgtcaggc gcgggctgtg tttctgcggc caggcggggc atggaggcgg ctcggccctg
      781 gctctccagc cctggctgcg gctcggcgtc agggccactc agagtcatgt tctgcatctg
      841 gacatctaca gccagtgtcc caggctgccc gactgggatc tcagctgagt ggccccgctc
      901 caggggtgcc ctggcctcgc aaagcacatc ctgggtcctc tgagtgcccc aagaaaccga
      961 ttccttgatg ccgctctcct gggtttgtac ggccccgccg ttgctcaggg aggcagaact
     1021 cttctgccgc aggaaagccc gggctccacg cagcgccggc tgagtctgct ccagcgtggc
     1081 cttcagcggg tggaacaggg acacggggcc cagctgggac aggcctccgg gggcttgctg
     1141 agtctggtct ctgctggttt tgttctgttt gtagaagtcg aagatcatca gagctgcata
     1201 gactttgccc accgtcatct cgtcaggctt atggggtggt accagtaagt ccagggtctt
     1261 ctggggcaga ttggcccaca cacaggaaat ctccttcctc agctctgcgt cgcactggtg
     1321 ctgcttggtc ccagctggtg ccagcttgat gtccagcgct gtccgaatga gagccatcag
     1381 cgtggatgtg aagtgcaccg tcatgtcctc actggagatg ggcatgttca tgcggaccag
     1441 gcgcttgtac gcgactcgag cagggcactt cttccccagc cccaggggtg gggacatgtg
     1501 cttcagcatc tcaaacatgt cactgtaact gatgcgccca cacgcggccg ggtcgtattc
     1561 agcccagacc cggatgaact catccaggtg gtgaggccct aagatggaag agtcccgtgt
     1621 aaggtactca aaattgtcca tgatcacagc cacaaagagg ttcaacatca gaaaggagca
     1681 gaggaagatg aaggagacga agtaaaagta ggcaaagtcg ctgccgcact cgctggcatt
     1741 ggagtgctcg tcgcaggccc ggctgctgag gcacgacagc attatctcgt gccaggcctc
     1801 ccccgtggcg ctcctgaaca gcaacatcaa ggcctgcaga aacgtccgga agttattgtg
     1861 cctgttgatg ctggtgtcat cgtccagggc gatgttccca aaaacctgca tgccaatgat
     1921 ggcgtagatg aagaagagca tggcgatgag gagacacacg tagggcagcg ccttgaagga
     1981 ctgcacgaag gtccaaagca ggatgcggat ggtgtagccc tggcgcagca gcttgatgag
     2041 ccgcgcggcc cggaacaggc gcaggaagct gaggttgatg aagttgttcg caatctctgt
     2101 taccaaaata tcagtaatac ttcccaacac cgtgacaaag tcaaagacat tccaggcatc
     2161 tctgaaatag ttcagcaccc cgaaggcgat gatcttcagc acacactcca tggagaacat
     2221 ggacgtgaac acgatgttca ggcatttcag cattagctca tactcgtacg gcgcatcgta
     2281 gaacttcatc atcagcacca cagtgttgag ggcgatcata gccatgatga agtactcaaa
     2341 aggtggggac accacgaacg tccacgtctt atactggaac gactgcttgt tctggggcat
     2401 gtaccgcgtc aggggtctgg cgctgatggc aaagtcgatg caagccctct cgttcttctc
     2461 caggctgcac tcggacatca ccttgtcccc ctgctcctgg aaggtgatga tgatcaaggc
     2521 cacgaagatg ttgacaaaga agaagggaaa gaccacaaag tagaccacgt agaagatgga
     2581 gagctccatg cggtacccgg ggctcgggcc ctgctcctcg taggtggcat ccacagagtg
     2641 tttcagcacc atgggccacc cttcccctgt ggacaccgtg aagagcgtta gcagagccca
     2701 gagcacgttg tcatagtgga agtcatattt cttccactgc ctcggttgag cttccacttc
     2761 ctccttctca taatccaaat actgacccct gcagtcacgc tccagctcct tggattcgtc
     2821 cgtgcagtag aagaacttcc ctttgaagag ctgcacggca atgacggcaa agatgaacat
     2881 gaagagcatg tagacgatca ggatgttgag gacgtttttc agtgagttca ccacacagtc
     2941 aaacacggcc ttgagcttgg gcagccgctt gatggtcttg agtggccgca gcacacgcag
     3001 gactctcagg gatttgatgg tgctgatgtc tttccctttg gatcctgaga aagcaaatgc
     3061 caccagggcc ccactgacca caatgaagtc cagaatgttc cacagatccc ggaaataggc
     3121 tccagggtgt agcagcagtc ccaaatcgat catctttatc accatctcga aggtgaagac
     3181 gcctgtgaag atgtagtcca tgtacttcag agcattattc ctgggagagt ctgttctcac
     3241 ggggtcctcg gcagccaggg cgatgctgct cagggcgatg accacgagga tgaccatctc
     3301 gaagtatcgc atggtcacaa tgtagtggca gcagcggcgc agcaggttgg ttgggcttaa
     3361 acagaacatg gaactgtacg ggacaatggg ccgagggccg ctcctcatca catcgctggt
     3421 ttccacctcc ttcttcccct ctgcttgact ttccaggtcc acgtttccac tgggcactac
     3481 cgtggtctcc ccgggggggc cggtcagcgt cacaggaatg tgcacagtgg tgctggtgtc
     3541 cgggggctga ctacccatgc gggtgacgtt ccgctggttg tccgcatcct ctggctgctc
     3601 ctccaccttc tgcagacagg tgctgggcag cgtatgcaca gcgcccactc ccagcatgcc
     3661 gatggcctcc aggtcacagg gcagctcctt gggctggtgg ttccgagcct ccttgtcctt
     3721 ctcggcctcc gtggcctcgc cccccttctc ggcagcctcc ttgtccttct ccgcggtctc
     3781 ctgggtgggc ggcgccttgt gccgagcccg gtgccgccgc gcaggctcct cgctgctctc
     3841 agcctcccgg gggcacgccc gggggcccgt gcggtgtcgt gcgcgccgct cgcccctggt
     3901 cccggacgct gggccctcct tgcccgggtc gggcccgtgc cggtgcgcgc ggtggcggcg
     3961 gggctccctc tcagctgcct cctccggtga cccgcgccgg tggtggcgcc gaccaccttc
     4021 aggacacggg ccacggccgc gatcgctgcg cacctccggg gcgcccgggg cctccttgct
     4081 gcggctgcgg cgtggccgcc cccgctcctc gcggggcccc agctccccgc cctctgccct
     4141 cagagcttcg gctctgtcct gttcccccgc cgaggggacc gtggcgggag ccttgtcctt
     4201 gtctcggtgc cggtgatgcc tgcgcggggg atccgcaccc tcgggggcct ccgagccatc
     4261 cggccgggac ttcccccccg gggggccccg cggagcgtcg cggccgggct caaccaccag
     4321 cggccggtcc aggtgcgtct tcacgtcggg ccgcaggtgg cgcgcggtgg cgtagcgcag
     4381 cctctcctcg gggtccatct cgctgtacag cgcctcgcag ctggccctca ggttctgcag
     4441 ccgcagctgg ctggctcgct gctcccacac cgagcgcgct ttggccgagt tctgctgcct
     4501 ggcggcaatg gagatgttcg cagcagacat ggggctgact tcagccactt ccttggcctt
     4561 ctgcaaggcg agtttctgat tggctgcttc ttccatctcc tcttcatcct tggtcagctc
     4621 ctgagcattg gcaaggttgt ccacagcaat ggccaggaag acattcagca gggtgtagtt
     4681 cccaaacaga gtgaggacaa tgaagtagaa ggaggagaac attcctctgc tgacgccacc
     4741 ttgcgactcg atcccgtggt acatgaccgc attccagtcc tctccggtca ggatctggaa
     4801 gacggtgagg atagcagcag ggaaggtgtc aaagttggtc gttggggtct cgtctttaaa
     4861 gttgaactgg cctccaaaca gctgcatccc cagcagagca aagacaacaa tgaacaggaa
     4921 gagcaggaag aggaggctga tgatggactt catggagttg agcagagaca ccaccaggtt
     4981 ccgtagggag ttccagtatt tggtgacctt gaagattctc agtaagcgca gagcccgcag
     5041 aacactgatc ccgaaggagg tccctggctt gacagcagcc cagaccactt caaagatact
     5101 gcccacgatg accccaaagt caaaacagtt gaaggaggac cggaagtagc tcctgggccc
     5161 caggccatac atcttcaggg acatctctgt gaggaagaga cccaggaaaa caaactctgc
     5221 aaagtacaat gccgtggtga gccgctgcgg ctggttgtag tgcaccatgg ccacgcacag
     5281 tgtgttcagt gccaccacac acagcaccgt ccagtagaag ctctgtgcct tcaccatgcg
     5341 ccggatgaag aagcggaaca tcttctcctt cctccggaag tacgacgagc tctccgtctt
     5401 cccgcttttc aggctggcac gggcgaacgg ggaaccggga gcacagaggc ctgtgagccg
     5461 gccctccccc tcctcggcct ggatcaggtc gcttctgctc ttcttggcag ctgctctctt
     5521 caacactgca tccagagggg acttctcctc tgcgttcctg tcctcctcag ccagcatgac
     5581 ttcctcagcc ttgaagatcc actccaggta cccgttgagc tcccgctcaa tctgctgctg
     5641 ccgccggagc ttcaggaagg cgcggcggtt ctccactctc tcgcgctcct tagcaaactc
     5701 tcctgagagc acacccagca ccaggttgag catgaagaag gagccgatga tgatgagagg
     5761 gatgaagtag agccagttcc acgtgttgcc ggctgcatcg tttgtattgt agaggatgtc
     5821 agtccagccc tccatggtga tgcactggaa cacggtcaag atggcaaaca ggatgttgtc
     5881 aaagttggtg ataccaaagt tgggtccggc ccagtactcc cggcactccg tgtctccctc
     5941 acagagccgg gccggggcct ccttgccaca ggggaagtca cccacgggat ctgggtctgt
     6001 gctgttgggg aagcaggcct tgtggaactt gcccatgtag aactcgaggc cgatgatggc
     6061 aaacatgagg atggcgaaga agagcagcag cccgatctgc agcagcggga ccatggcctt
     6121 catgatggac ttgagcacca cctgcaagct cggaatccca gacaccagct tcaggggcct
     6181 tagcacacgc acagccctca gcgttcgcag gtcgaagtcg gttccggctg tggcaagaat
     6241 ccccgtgagg actaccacga agtccatgac gttccagccg ttccgcagat aggagccctt
     6301 gtgcagaaca aagcccaggg ccaggatctt gatccccgcc tcgaagcaga agatcccaat
     6361 gaagtagggc tcggtgtcat ccaggcgctc ggacataggc gtcttgtccc cgtcagggag
     6421 gtgctgctcc agggccagca cgatgcagtt ggcgatgatg gtggccagga tcatgtactc
     6481 gaacggaggc cactcggtga tgcgcttggc gtatttgcgg acgacgttgt cctcgctgaa
     6541 gacgaagagc gagcggttga cggtgaagca gttctgcttg accgggatgg ggttgtacag
     6601 cgccatggtc cgcgcgcgct gcgcgatcga ctgtttgtag aggacccgct ggcccggcgg
     6661 cagccccccg gggcccgggc cgccagcccc gccggccccg ccgccccggg cccgctccgc
     6721 gccgcccgcg cccccatagc ggccgcccag ctcgtccccg aagcggacca tgatcaagct
     6781 tcgacctcga gggggggccc ggtaccctcg aggaattcca gcacactggc ggccgttact
     6841 agtggatccg agctcggtac caagcttggg tctccctata gtgagtcgta ttaatttcga
     6901 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
     6961 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     7021 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     7081 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     7141 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     7201 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     7261 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     7321 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     7381 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     7441 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     7501 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
     7561 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     7621 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     7681 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag
     7741 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7921 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7981 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8041 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8101 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8161 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8221 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8281 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8341 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8401 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8461 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8521 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8581 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8641 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8701 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8761 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8821 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9661 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9721 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9781 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9841 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9901 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg
     9961 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
    10021 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
    10081 gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
    10141 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
    10201 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
    10261 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
    10321 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
    10381 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
    10441 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
    10501 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
    10561 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
    10621 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
    10681 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
    10741 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
    10801 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
    10861 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
    10921 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
    10981 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
    11041 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatccgaa aatggatata
    11101 caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
    11161 tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
    11221 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
    11281 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
    11341 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
    11401 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagaatt
    11461 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    11521 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
    11581 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
    11641 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
    11701 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    11761 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    11821 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    11881 agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca gaagccatag
    11941 agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct
    12001 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
    12061 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
    12121 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgagctc
    12181 tagcatttag gtgacactat agaatagggc cctctagatg catgctcgag cggccgccag
    12241 tgtgatggat atctgcagaa ttcgtcgaag cttgctagaa ctagtctagc accggcggtc
    12301 gcggtcgggg tggtggcagc cgcgccgcgc ccggccgccc ccgcccagcc ccagagtgca
    12361 gtggtagccg ttgggcacgc ggcgcagcgg cgggggctgc gaggtcagtg aggacacgta
    12421 cgaggtccgg gaggagcggc ccgagttgct ggccgcggtc tcctcaaagg cgggcgcgtc
    12481 ctcgggcagg gcacgggcgg gcgcctcgct gtccagccgc tgccccaggt aggg
//
