LOCUS       sequence-406763-       13302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal GFP 
            tag and an exofacial double HA tag in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Macabuag N, Dolphin AC
  TITLE     Alternative Splicing in Ca(V)2.2 Regulates Neuronal Trafficking via 
            Adaptor Protein Complex-1 Adaptor Protein Motifs.
  JOURNAL   J Neurosci. 2015 Oct 28;35(43):14636-52. doi: 
            10.1523/JNEUROSCI.3034-15.2015.
  PUBMED    26511252
REFERENCE   2  (bases 1 to 13302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3034-15"; journalName: "J Neurosci"; date: 
            "2015-10-28- 28"; volume: "35"; issue: "43"; pages: "14636-52"
COMMENT     Sequence Label: GFP Cav2.2 HA pcDNA3
FEATURES             Location/Qualifiers
     source          1..13302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             973..1686
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             973..1686
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1003..1031)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1626..1646
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3385..3411
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3415..3441
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(8855..8873)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8856..8873)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8893..8910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8899..9123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9169..9597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9256..9275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9466..9487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9606..9626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9611..9940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9791..9926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9853..9872
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10007..10801
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10061..10080)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10671..10690
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10975..11096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11012..11031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11066..11085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11145..11161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11145..11161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11158..11180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11169..11185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11193..11199,11200..11217,11218..11223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11238..11259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11376..11393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11547..12135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11627..11646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12306..13097,13098..13166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12929..12948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13167..13271)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcctcgagg gtaccgagct
      961 cggatctaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1021 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1081 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1141 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1201 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1261 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1321 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1381 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1441 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1501 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1561 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1621 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1681 tacaaaatgg tccgcttcgg ggacgagctg ggcggccgct atgggggcgc gggcggcgcg
     1741 gagcgggccc ggggcggcgg ggccggcggg gctggcggcc cgggccccgg ggggctgccg
     1801 ccgggccagc gggtcctcta caaacagtcg atcgcgcagc gcgcgcggac catggcgctg
     1861 tacaacccca tcccggtcaa gcagaactgc ttcaccgtca accgctcgct cttcgtcttc
     1921 agcgaggaca acgtcgtccg caaatacgcc aagcgcatca ccgagtggcc tccgttcgag
     1981 tacatgatcc tggccaccat catcgccaac tgcatcgtgc tggccctgga gcagcacctc
     2041 cctgacgggg acaagacgcc tatgtccgag cgcctggatg acaccgagcc ctacttcatt
     2101 gggatcttct gcttcgaggc ggggatcaag atcctggccc tgggctttgt tctgcacaag
     2161 ggctcctatc tgcggaacgg ctggaacgtc atggacttcg tggtagtcct cacggggatt
     2221 cttgccacag ccggaaccga cttcgacctg cgaacgctga gggctgtgcg tgtgctaagg
     2281 cccctgaagc tggtgtctgg gattccgagc ttgcaagtgg tgctcaagtc catcatgaag
     2341 gccatggtcc cgctgctgca gatcgggctg ctgctcttct tcgccatcct catgtttgcc
     2401 atcatcggcc tcgagttcta catgggcaag ttccacaagg cctgcttccc caacagcaca
     2461 gacccagatc ccgtgggtga cttcccctgt ggcaaggagg ccccggcccg gctctgtgag
     2521 ggagacacgg agtgccggga gtactgggcc ggacccaact ttggtatcac caactttgac
     2581 aacatcctgt ttgccatctt gaccgtgttc cagtgcatca ccatggaggg ctggactgac
     2641 atcctctaca atacaaacga tgcagccggc aacacgtgga actggctcta cttcatccct
     2701 ctcatcatca tcggctcctt cttcatgctc aacctggtgc tgggtgtgct ctcaggagag
     2761 tttgctaagg agcgcgagag agtggagaac cgccgcgcct tcctgaagct ccggcggcag
     2821 cagcagattg agcgggagct caacgggtac ctggagtgga tcttcaaggc tgaggaagtc
     2881 atgctggctg aggaggacag gaacgcagag gagaagtccc ctctggatgc agtgttgaag
     2941 agagcagctg ccaagaagag cagaagcgac ctgatccagg ccgaggaggg ggagggccgg
     3001 ctcacaggcc tctgtgctcc cggttccccg ttcgcccgtg ccagcctgaa aagcgggaag
     3061 acggagagct cgtcgtactt ccggaggaag gagaagatgt tccgcttctt catccggcgc
     3121 atggtgaagg cacagagctt ctactggacg gtgctgtgtg tggtggcact gaacacactg
     3181 tgcgtggcca tggtgcacta caaccagccg cagcggctca ccacggcatt gtactttgca
     3241 gagtttgttt tcctgggtct cttcctcaca gagatgtccc tgaagatgta tggcctgggg
     3301 cccaggagct acttccggtc ctccttcaac tgttttgact ttggggtcat cgtgggcagt
     3361 atctttgaag tggtctgggc tgcttacccc tacgacgtcc ccgactacgc cggctacccg
     3421 tacgacgtcc cggactacgc cggcgtcgac gtcaagccag ggacctcctt cgggatcagt
     3481 gttctgcggg ctctgcgctt actgagaatc ttcaaggtca ccaaatactg gaactcccta
     3541 cggaacctgg tggtgtctct gctcaactcc atgaagtcca tcatcagcct cctcttcctg
     3601 ctcttcctgt tcattgttgt ctttgctctg ctggggatgc agctgtttgg aggccagttc
     3661 aactttaaag acgagacccc aacgaccaac tttgacacct tccctgctgc tatcctcacc
     3721 gtcttccaga tcctgaccgg agaggactgg aatgcggtca tgtaccacgg gatcgagtcg
     3781 caaggtggcg tcagcagagg aatgttctcc tccttctact tcattgtcct cactctgttt
     3841 gggaactaca ccctgctgaa tgtcttcctg gccattgctg tggacaacct tgccaatgct
     3901 caggagctga ccaaggatga agaggagatg gaagaagcag ccaatcagaa actcgccttg
     3961 cagaaggcca aggaagtggc tgaagtcagc cccatgtctg ctgcgaacat ctccattgcc
     4021 gccaggcagc agaactcggc caaagcgcgc tcggtgtggg agcagcgagc cagccagctg
     4081 cggctgcaga acctgagggc cagctgcgag gcgctgtaca gcgagatgga ccccgaggag
     4141 aggctgcgct acgccaccgc gcgccacctg cggcccgacg tgaagacgca cctggaccgg
     4201 ccgctggtgg ttgagcccgg ccgcgacgct ccgcggggcc ccccgggggg gaagtcccgg
     4261 ccggatggct cggaggcccc cgagggtgcg gatcccccgc gcaggcatca ccggcaccga
     4321 gacaaggaca aggctcccgc cacggtcccc tcggcggggg aacaggacag agccgaagct
     4381 ctgagggcag agggcgggga gctggggccc cgcgaggagc gggggcggcc acgccgcagc
     4441 cgcagcaagg aggccccggg cgccccggag gtgcgcagcg atcgcggccg tggcccgtgt
     4501 cctgaaggtg gtcggcgcca ccaccggcgc gggtcaccgg aggaggcagc tgagagggag
     4561 ccccgccgcc accgcgcgca ccggcacggg cccgacccgg gcaaggaggg cccagcgtcc
     4621 gggaccaggg gcgagcggcg cgcacgacac cgcacgggcc cccgggcgtg cccccgggag
     4681 gctgagagca gcgaggagcc tgcgcggcgg caccgggctc ggcacaaggc gccgcccacc
     4741 caggagaccg cggagaagga caaggaggct gccgagaagg ggggcgaggc cacggaggcc
     4801 gagaaggaca aggaggctcg gaaccaccag cccaaggagc tgccctgtga cctggaggcc
     4861 atcggcatgc tgggagtggg cgctgtgcat acgctgccca gcacctgtct gcagaaggtg
     4921 gaggagcagc cagaggatgc ggacaaccag cggaacgtca cccgcatggg tagtcagccc
     4981 ccggacacca gcaccactgt gcacattcct gtgacgctga ccggcccccc cggggagacc
     5041 acggtagtgc ccagtggaaa cgtggacctg gaaagtcaag cagaggggaa gaaggaggtg
     5101 gaaaccagcg atgtgatgag gagcggccct cggcccattg tcccgtacag ttccatgttc
     5161 tgtttaagcc caaccaacct gctgcgccgc tgctgccact acattgtgac catgcgatac
     5221 ttcgagatgg tcatcctcgt ggtcatcgcc ctgagcagca tcgccctggc tgccgaggac
     5281 cccgtgagaa cagactctcc caggaataat gctctgaagt acatggacta catcttcaca
     5341 ggcgtcttca ccttcgagat ggtgataaag atgatcgatt tgggactgct gctacaccct
     5401 ggagcctatt tccgggatct gtggaacatt ctggacttca ttgtggtcag tggggccctg
     5461 gtggcatttg ctttctcagg atccaaaggg aaagacatca gcaccatcaa atccctgaga
     5521 gtcctgcgtg tgctgcggcc actcaagacc atcaagcggc tgcccaagct caaggccgtg
     5581 tttgactgtg tggtgaactc actgaaaaac gtcctcaaca tcctgatcgt ctacatgctc
     5641 ttcatgttca tctttgccgt cattgccgtg cagctcttca aagggaagtt cttctactgc
     5701 acggacgaat ccaaggagct ggagcgtgac tgcaggggtc agtatttgga ttatgagaag
     5761 gaggaagtgg aagctcaacc gaggcagtgg aagaaatatg acttccacta tgacaacgtg
     5821 ctctgggctc tgctaacgct cttcacggtg tccacagggg aagggtggcc catggtgctg
     5881 aaacactctg tggatgccac ctacgaggag cagggcccga gccccgggta ccgcatggag
     5941 ctctccatct tctacgtggt ctactttgtg gtctttccct tcttctttgt caacatcttc
     6001 gtggccttga tcatcatcac cttccaggag cagggggaca aggtgatgtc cgagtgcagc
     6061 ctggagaaga acgagagggc ttgcatcgac tttgccatca gcgccagacc cctgacgcgg
     6121 tacatgcccc agaacaagca gtcgttccag tataagacgt ggacgttcgt ggtgtcccca
     6181 ccttttgagt acttcatcat ggctatgatc gccctcaaca ctgtggtgct gatgatgaag
     6241 ttctacgatg cgccgtacga gtatgagcta atgctgaaat gcctgaacat cgtgttcacg
     6301 tccatgttct ccatggagtg tgtgctgaag atcatcgcct tcggggtgct gaactatttc
     6361 agagatgcct ggaatgtctt tgactttgtc acggtgttgg gaagtattac tgatattttg
     6421 gtaacagaga ttgcgaacaa cttcatcaac ctcagcttcc tgcgcctgtt ccgggccgcg
     6481 cggctcatca agctgctgcg ccagggctac accatccgca tcctgctttg gaccttcgtg
     6541 cagtccttca aggcgctgcc ctacgtgtgt ctcctcatcg ccatgctctt cttcatctac
     6601 gccatcattg gcatgcaggt ttttgggaac atcgccctgg acgatgacac cagcatcaac
     6661 aggcacaata acttccggac gtttctgcag gccttgatgt tgctgttcag gagcgccacg
     6721 ggggaggcct ggcacgagat aatgctgtcg tgcctcagca gccgggcctg cgacgagcac
     6781 tccaatgcca gcgagtgcgg cagcgacttt gcctactttt acttcgtctc cttcatcttc
     6841 ctctgctcct ttctgatgtt gaacctcttt gtggctgtga tcatggacaa ttttgagtac
     6901 cttacacggg actcttccat cttagggcct caccacctgg atgagttcat ccgggtctgg
     6961 gctgaatacg acccggccgc gtgtgggcgc atcagttaca gtgacatgtt tgagatgctg
     7021 aagcacatgt ccccacccct ggggctgggg aagaagtgcc ctgctcgagt cgcgtacaag
     7081 cgcctggtcc gcatgaacat gcccatctcc agtgaggaca tgacggtgca cttcacatcc
     7141 acgctgatgg ctctcattcg gacagcgctg gacatcaagc tggcaccagc tgggaccaag
     7201 cagcaccagt gcgacgcaga gctgaggaag gagatttcct gtgtgtgggc caatctgccc
     7261 cagaagaccc tggacttact ggtaccaccc cataagcctg acgagatgac ggtgggcaaa
     7321 gtctatgcag ctctgatgat cttcgacttc tacaaacaga acaaaaccag cagagaccag
     7381 actcagcaag cccccggagg cctgtcccag ctgggccccg tgtccctgtt ccacccgctg
     7441 aaggccacgc tggagcagac tcagccggcg ctgcgtggag cccgggcttt cctgcggcag
     7501 aagagttctg cctccctgag caacggcggg gccgtacaaa cccaggagag cggcatcaag
     7561 gaatcggttt cttggggcac tcagaggacc caggatgtgc tttgcgaggc cagggcaccc
     7621 ctggagcggg gccactcagc tgagatccca gtcgggcagc ctgggacact ggctgtagat
     7681 gtccagatgc agaacatgac tctgagtggc cctgacgccg agccgcagcc agggctggag
     7741 agccagggcc gagccgcctc catgccccgc ctggccgcag aaacacagcc cgcgcctgac
     7801 gccagcccca tgaagcgctc catctccacg ctggccccgc gcccccacac ggcccggctc
     7861 ggcagcaccg cgctggaccg cccagccccc agccaggccc cgcaccatca ccaccaccgc
     7921 tgccaccgcc gcagggacag gaagcagcgg tccctggaga agggtcccag cctgtctgct
     7981 gacacagatg gggcccccga cagcacggtg gggccaggac tgcccacggg ggagggcccc
     8041 cccggctgca ggcgggagcg ggagcgcagg caggagcggg gccggtccca ggagcggagg
     8101 cagccctcgt cctcctcctc ggagaagcat cgcttctact cctgtgaccg cttcgggggc
     8161 cgcgaacccc cacagcccaa gccctccctc agtagtcacc ccacgtcgcc gacggccggg
     8221 caggagccgg gaccccaccc gcaggggagt ggttcggttc acgggagccc cctgctgtcc
     8281 acatctggtg ctagcacccc gggccggggg cggaggcagc tcccccagac ccccttgacc
     8341 ccccgcccca gcgtcaccta caagacggcc aactcctcgc ctgtccactt cgccggggca
     8401 ccgagcggcc tccccgcctt ctcccccggc cggctcagcc ggggcctctc agaacacaac
     8461 gccctgctgc agagggaccc gctcagccgg cccctggcgc ccggctcgcg catcggctcc
     8521 gacccctacc tggggcagcg gctggacagc gaggcgcccg cccgtgccct gcccgaggac
     8581 gcgcccgcct ttgaggagac cgcggccagc aactcgggcc gctcctcccg gacctcgtac
     8641 gtgtcctcac tgacctcgca gcccccgccg ctgcgccgcg tgcccaacgg ctaccactgc
     8701 actctggggc tgggcggggg cggccgggcg cggcgcggct gccaccaccc cgaccgcgac
     8761 cgccggtgct agactagttc tagcaagctt cgacgaattc tgcagatatc catcacactg
     8821 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     8881 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8941 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     9001 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     9061 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     9121 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     9181 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9241 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9301 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9361 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9421 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9481 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9541 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     9601 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9661 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9721 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9841 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     9901 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     9961 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
    10021 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
    10081 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
    10141 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
    10201 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    10261 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    10321 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    10381 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    10441 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    10501 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
    10561 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    10621 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    10681 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    10741 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    10801 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
    10861 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10921 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    10981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11101 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    11161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    11221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    11281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    11341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    11401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    11461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    11521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    11941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    12001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12421 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    12961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13261 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
