LOCUS       sequence-406766-       12600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Cassidy JS, Ferron L, Kadurin I, Pratt WS, Dolphin AC
  TITLE     Functional exofacially tagged N-type calcium channels elucidate the 
            interaction with auxiliary alpha2delta-1 subunits.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8979-84. doi: 
            10.1073/pnas.1403731111. Epub 2014 Jun 2.
  PUBMED    24889613
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1403731111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8979-84"
COMMENT     Sequence Label: Cav2.2 HA pcDNA3
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(800..818)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(801..818)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(838..855)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    844..1068
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1114..1542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1201..1220)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1411..1432
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1551..1571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1556..1885
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1736..1871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1798..1817
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1952..2746
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2006..2025)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2616..2635
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2920..3041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2957..2976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3011..3030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3090..3106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3090..3106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3103..3125)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3114..3130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3138..3144,3145..3162,3163..3168))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3183..3204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3321..3338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3492..4080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3572..3591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4251..5042,5043..5111))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4874..4893
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5112..5216)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5291..5310)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5482..5861
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5862..6065
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6016..6036
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6110..6129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6110..6128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7930..7956
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7960..7986
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagcgggagc gcaggcagga gcggggccgg tcccaggagc ggaggcagcc ctcgtcctcc
       61 tcctcggaga agcatcgctt ctactcctgt gaccgcttcg ggggccgcga acccccacag
      121 cccaagccct ccctcagtag tcaccccacg tcgccgacgg ccgggcagga gccgggaccc
      181 cacccgcagg ggagtggttc ggttcacggg agccccctgc tgtccacatc tggtgctagc
      241 accccgggcc gggggcggag gcagctcccc cagaccccct tgaccccccg ccccagcgtc
      301 acctacaaga cggccaactc ctcgcctgtc cacttcgccg gggcaccgag cggcctcccc
      361 gccttctccc ccggccggct cagccggggc ctctcagaac acaacgccct gctgcagagg
      421 gacccgctca gccggcccct ggcgcccggc tcgcgcatcg gctccgaccc ctacctgggg
      481 cagcggctgg acagcgaggc gcccgcccgt gccctgcccg aggacgcgcc cgcctttgag
      541 gagaccgcgg ccagcaactc gggccgctcc tcccggacct cgtacgtgtc ctcactgacc
      601 tcgcagcccc cgccgctgcg ccgcgtgccc aacggctacc actgcactct ggggctgggc
      661 gggggcggcc gggcgcggcg cggctgccac caccccgacc gcgaccgccg gtgctagact
      721 agttctagca agcttcgacg aattctgcag atatccatca cactggcggc cgctcgagca
      781 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
      841 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      901 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      961 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1021 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     1081 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     1141 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1201 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     1261 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     1321 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     1381 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1441 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     1501 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     1561 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1621 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     1681 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     1741 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     1801 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     1861 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     1921 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     1981 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     2041 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2101 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     2161 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2221 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     2281 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2341 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2401 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     2461 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     2521 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     2581 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     2641 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     2701 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     2761 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     2821 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     2881 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     2941 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3001 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     3061 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3121 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3181 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3241 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3301 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3361 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3421 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3481 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3541 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3601 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3661 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3841 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3901 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3961 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4021 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4081 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4141 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4201 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4261 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4321 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4381 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4441 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4501 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4561 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4621 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4681 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4741 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4801 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4861 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4921 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4981 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5041 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5101 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5161 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5221 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     5281 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     5341 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     5401 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     5461 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     5521 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     5581 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     5641 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     5701 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     5761 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     5821 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     5881 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     5941 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     6001 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     6061 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     6121 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
     6181 gctggaattc ctcgagggta ccgggccccc cctcgaggtc gaagcttgat catggtccgc
     6241 ttcggggacg agctgggcgg ccgctatggg ggcgcgggcg gcgcggagcg ggcccggggc
     6301 ggcggggccg gcggggctgg cggcccgggc cccggggggc tgccgccggg ccagcgggtc
     6361 ctctacaaac agtcgatcgc gcagcgcgcg cggaccatgg cgctgtacaa ccccatcccg
     6421 gtcaagcaga actgcttcac cgtcaaccgc tcgctcttcg tcttcagcga ggacaacgtc
     6481 gtccgcaaat acgccaagcg catcaccgag tggcctccgt tcgagtacat gatcctggcc
     6541 accatcatcg ccaactgcat cgtgctggcc ctggagcagc acctccctga cggggacaag
     6601 acgcctatgt ccgagcgcct ggatgacacc gagccctact tcattgggat cttctgcttc
     6661 gaggcgggga tcaagatcct ggccctgggc tttgttctgc acaagggctc ctatctgcgg
     6721 aacggctgga acgtcatgga cttcgtggta gtcctcacgg ggattcttgc cacagccgga
     6781 accgacttcg acctgcgaac gctgagggct gtgcgtgtgc taaggcccct gaagctggtg
     6841 tctgggattc cgagcttgca agtggtgctc aagtccatca tgaaggccat ggtcccgctg
     6901 ctgcagatcg ggctgctgct cttcttcgcc atcctcatgt ttgccatcat cggcctcgag
     6961 ttctacatgg gcaagttcca caaggcctgc ttccccaaca gcacagaccc agatcccgtg
     7021 ggtgacttcc cctgtggcaa ggaggccccg gcccggctct gtgagggaga cacggagtgc
     7081 cgggagtact gggccggacc caactttggt atcaccaact ttgacaacat cctgtttgcc
     7141 atcttgaccg tgttccagtg catcaccatg gagggctgga ctgacatcct ctacaataca
     7201 aacgatgcag ccggcaacac gtggaactgg ctctacttca tccctctcat catcatcggc
     7261 tccttcttca tgctcaacct ggtgctgggt gtgctctcag gagagtttgc taaggagcgc
     7321 gagagagtgg agaaccgccg cgccttcctg aagctccggc ggcagcagca gattgagcgg
     7381 gagctcaacg ggtacctgga gtggatcttc aaggctgagg aagtcatgct ggctgaggag
     7441 gacaggaacg cagaggagaa gtcccctctg gatgcagtgt tgaagagagc agctgccaag
     7501 aagagcagaa gcgacctgat ccaggccgag gagggggagg gccggctcac aggcctctgt
     7561 gctcccggtt ccccgttcgc ccgtgccagc ctgaaaagcg ggaagacgga gagctcgtcg
     7621 tacttccgga ggaaggagaa gatgttccgc ttcttcatcc ggcgcatggt gaaggcacag
     7681 agcttctact ggacggtgct gtgtgtggtg gcactgaaca cactgtgcgt ggccatggtg
     7741 cactacaacc agccgcagcg gctcaccacg gcattgtact ttgcagagtt tgttttcctg
     7801 ggtctcttcc tcacagagat gtccctgaag atgtatggcc tggggcccag gagctacttc
     7861 cggtcctcct tcaactgttt tgactttggg gtcatcgtgg gcagtatctt tgaagtggtc
     7921 tgggctgctt acccctacga cgtccccgac tacgccggct acccgtacga cgtcccggac
     7981 tacgccggcg tcgacgtcaa gccagggacc tccttcggga tcagtgttct gcgggctctg
     8041 cgcttactga gaatcttcaa ggtcaccaaa tactggaact ccctacggaa cctggtggtg
     8101 tctctgctca actccatgaa gtccatcatc agcctcctct tcctgctctt cctgttcatt
     8161 gttgtctttg ctctgctggg gatgcagctg tttggaggcc agttcaactt taaagacgag
     8221 accccaacga ccaactttga caccttccct gctgctatcc tcaccgtctt ccagatcctg
     8281 accggagagg actggaatgc ggtcatgtac cacgggatcg agtcgcaagg tggcgtcagc
     8341 agaggaatgt tctcctcctt ctacttcatt gtcctcactc tgtttgggaa ctacaccctg
     8401 ctgaatgtct tcctggccat tgctgtggac aaccttgcca atgctcagga gctgaccaag
     8461 gatgaagagg agatggaaga agcagccaat cagaaactcg ccttgcagaa ggccaaggaa
     8521 gtggctgaag tcagccccat gtctgctgcg aacatctcca ttgccgccag gcagcagaac
     8581 tcggccaaag cgcgctcggt gtgggagcag cgagccagcc agctgcggct gcagaacctg
     8641 agggccagct gcgaggcgct gtacagcgag atggaccccg aggagaggct gcgctacgcc
     8701 accgcgcgcc acctgcggcc cgacgtgaag acgcacctgg accggccgct ggtggttgag
     8761 cccggccgcg acgctccgcg gggccccccg ggggggaagt cccggccgga tggctcggag
     8821 gcccccgagg gtgcggatcc cccgcgcagg catcaccggc accgagacaa ggacaaggct
     8881 cccgccacgg tcccctcggc gggggaacag gacagagccg aagctctgag ggcagagggc
     8941 ggggagctgg ggccccgcga ggagcggggg cggccacgcc gcagccgcag caaggaggcc
     9001 ccgggcgccc cggaggtgcg cagcgatcgc ggccgtggcc cgtgtcctga aggtggtcgg
     9061 cgccaccacc ggcgcgggtc accggaggag gcagctgaga gggagccccg ccgccaccgc
     9121 gcgcaccggc acgggcccga cccgggcaag gagggcccag cgtccgggac caggggcgag
     9181 cggcgcgcac gacaccgcac gggcccccgg gcgtgccccc gggaggctga gagcagcgag
     9241 gagcctgcgc ggcggcaccg ggctcggcac aaggcgccgc ccacccagga gaccgcggag
     9301 aaggacaagg aggctgccga gaaggggggc gaggccacgg aggccgagaa ggacaaggag
     9361 gctcggaacc accagcccaa ggagctgccc tgtgacctgg aggccatcgg catgctggga
     9421 gtgggcgctg tgcatacgct gcccagcacc tgtctgcaga aggtggagga gcagccagag
     9481 gatgcggaca accagcggaa cgtcacccgc atgggtagtc agcccccgga caccagcacc
     9541 actgtgcaca ttcctgtgac gctgaccggc ccccccgggg agaccacggt agtgcccagt
     9601 ggaaacgtgg acctggaaag tcaagcagag gggaagaagg aggtggaaac cagcgatgtg
     9661 atgaggagcg gccctcggcc cattgtcccg tacagttcca tgttctgttt aagcccaacc
     9721 aacctgctgc gccgctgctg ccactacatt gtgaccatgc gatacttcga gatggtcatc
     9781 ctcgtggtca tcgccctgag cagcatcgcc ctggctgccg aggaccccgt gagaacagac
     9841 tctcccagga ataatgctct gaagtacatg gactacatct tcacaggcgt cttcaccttc
     9901 gagatggtga taaagatgat cgatttggga ctgctgctac accctggagc ctatttccgg
     9961 gatctgtgga acattctgga cttcattgtg gtcagtgggg ccctggtggc atttgctttc
    10021 tcaggatcca aagggaaaga catcagcacc atcaaatccc tgagagtcct gcgtgtgctg
    10081 cggccactca agaccatcaa gcggctgccc aagctcaagg ccgtgtttga ctgtgtggtg
    10141 aactcactga aaaacgtcct caacatcctg atcgtctaca tgctcttcat gttcatcttt
    10201 gccgtcattg ccgtgcagct cttcaaaggg aagttcttct actgcacgga cgaatccaag
    10261 gagctggagc gtgactgcag gggtcagtat ttggattatg agaaggagga agtggaagct
    10321 caaccgaggc agtggaagaa atatgacttc cactatgaca acgtgctctg ggctctgcta
    10381 acgctcttca cggtgtccac aggggaaggg tggcccatgg tgctgaaaca ctctgtggat
    10441 gccacctacg aggagcaggg cccgagcccc gggtaccgca tggagctctc catcttctac
    10501 gtggtctact ttgtggtctt tcccttcttc tttgtcaaca tcttcgtggc cttgatcatc
    10561 atcaccttcc aggagcaggg ggacaaggtg atgtccgagt gcagcctgga gaagaacgag
    10621 agggcttgca tcgactttgc catcagcgcc agacccctga cgcggtacat gccccagaac
    10681 aagcagtcgt tccagtataa gacgtggacg ttcgtggtgt ccccaccttt tgagtacttc
    10741 atcatggcta tgatcgccct caacactgtg gtgctgatga tgaagttcta cgatgcgccg
    10801 tacgagtatg agctaatgct gaaatgcctg aacatcgtgt tcacgtccat gttctccatg
    10861 gagtgtgtgc tgaagatcat cgccttcggg gtgctgaact atttcagaga tgcctggaat
    10921 gtctttgact ttgtcacggt gttgggaagt attactgata ttttggtaac agagattgcg
    10981 aacaacttca tcaacctcag cttcctgcgc ctgttccggg ccgcgcggct catcaagctg
    11041 ctgcgccagg gctacaccat ccgcatcctg ctttggacct tcgtgcagtc cttcaaggcg
    11101 ctgccctacg tgtgtctcct catcgccatg ctcttcttca tctacgccat cattggcatg
    11161 caggtttttg ggaacatcgc cctggacgat gacaccagca tcaacaggca caataacttc
    11221 cggacgtttc tgcaggcctt gatgttgctg ttcaggagcg ccacggggga ggcctggcac
    11281 gagataatgc tgtcgtgcct cagcagccgg gcctgcgacg agcactccaa tgccagcgag
    11341 tgcggcagcg actttgccta cttttacttc gtctccttca tcttcctctg ctcctttctg
    11401 atgttgaacc tctttgtggc tgtgatcatg gacaattttg agtaccttac acgggactct
    11461 tccatcttag ggcctcacca cctggatgag ttcatccggg tctgggctga atacgacccg
    11521 gccgcgtgtg ggcgcatcag ttacagtgac atgtttgaga tgctgaagca catgtcccca
    11581 cccctggggc tggggaagaa gtgccctgct cgagtcgcgt acaagcgcct ggtccgcatg
    11641 aacatgccca tctccagtga ggacatgacg gtgcacttca catccacgct gatggctctc
    11701 attcggacag cgctggacat caagctggca ccagctggga ccaagcagca ccagtgcgac
    11761 gcagagctga ggaaggagat ttcctgtgtg tgggccaatc tgccccagaa gaccctggac
    11821 ttactggtac caccccataa gcctgacgag atgacggtgg gcaaagtcta tgcagctctg
    11881 atgatcttcg acttctacaa acagaacaaa accagcagag accagactca gcaagccccc
    11941 ggaggcctgt cccagctggg ccccgtgtcc ctgttccacc cgctgaaggc cacgctggag
    12001 cagactcagc cggcgctgcg tggagcccgg gctttcctgc ggcagaagag ttctgcctcc
    12061 ctgagcaacg gcggggccgt acaaacccag gagagcggca tcaaggaatc ggtttcttgg
    12121 ggcactcaga ggacccagga tgtgctttgc gaggccaggg cacccctgga gcggggccac
    12181 tcagctgaga tcccagtcgg gcagcctggg acactggctg tagatgtcca gatgcagaac
    12241 atgactctga gtggccctga cgccgagccg cagccagggc tggagagcca gggccgagcc
    12301 gcctccatgc cccgcctggc cgcagaaaca cagcccgcgc ctgacgccag ccccatgaag
    12361 cgctccatct ccacgctggc cccgcgcccc cacacggccc ggctcggcag caccgcgctg
    12421 gaccgcccag cccccagcca ggccccgcac catcaccacc accgctgcca ccgccgcagg
    12481 gacaggaagc agcggtccct ggagaagggt cccagcctgt ctgctgacac agatggggcc
    12541 cccgacagca cggtggggcc aggactgccc acgggggagg gcccccccgg ctgcaggcgg
//
