LOCUS       sequence-406767-       12639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            Bungarotoxin Binding Site motif in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Cassidy JS, Ferron L, Kadurin I, Pratt WS, Dolphin AC
  TITLE     Functional exofacially tagged N-type calcium channels elucidate the 
            interaction with auxiliary alpha2delta-1 subunits.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8979-84. doi: 
            10.1073/pnas.1403731111. Epub 2014 Jun 2.
  PUBMED    24889613
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1403731111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8979-84"
COMMENT     Sequence Label: Cav2.2 BBS pcDNA3
FEATURES             Location/Qualifiers
     source          1..12639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6935..6954)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6936..6954)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6999..7202)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7028..7048)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7203..7582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7754..7773
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7848..7952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7953..8021,8022..8813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8171..8190)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8984..9572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9473..9492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9726..9743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9860..9881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9896..9901,9902..9919,9920..9926)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9934..9950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9939..9961
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9958..9974
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9958..9974
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10023..10144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10034..10053)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10088..10107
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10318..11112)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10429..10448)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11039..11058
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(11179..11508)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11193..11328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11247..11266)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     11493..11513
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(11522..11950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11632..11653)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11844..11863
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    11996..12220
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12209..12226
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        12246..12264
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     12246..12263
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ggtccctctg cagcagggcg ttgtgttctg agaggccccg gctgagccgg ccgggggaga
       61 aggcggggag gccgctcggt gccccggcga agtggacagg cgaggagttg gccgtcttgt
      121 aggtgacgct ggggcggggg gtcaaggggg tctgggggag ctgcctccgc ccccggcccg
      181 gggtgctagc accagatgtg gacagcaggg ggctcccgtg aaccgaacca ctcccctgcg
      241 ggtggggtcc cggctcctgc ccggccgtcg gcgacgtggg gtgactactg agggagggct
      301 tgggctgtgg gggttcgcgg cccccgaagc ggtcacagga gtagaagcga tgcttctccg
      361 aggaggagga cgagggctgc ctccgctcct gggaccggcc ccgctcctgc ctgcgctccc
      421 gctcccgcct gcagccgggg gggccctccc ccgtgggcag tcctggcccc accgtgctgt
      481 cgggggcccc atctgtgtca gcagacaggc tgggaccctt ctccagggac cgctgcttcc
      541 tgtccctgcg gcggtggcag cggtggtggt gatggtgcgg ggcctggctg ggggctgggc
      601 ggtccagcgc ggtgctgccg agccgggccg tgtgggggcg cggggccagc gtggagatgg
      661 agcgcttcat ggggctggcg tcaggcgcgg gctgtgtttc tgcggccagg cggggcatgg
      721 aggcggctcg gccctggctc tccagccctg gctgcggctc ggcgtcaggg ccactcagag
      781 tcatgttctg catctggaca tctacagcca gtgtcccagg ctgcccgact gggatctcag
      841 ctgagtggcc ccgctccagg ggtgccctgg cctcgcaaag cacatcctgg gtcctctgag
      901 tgccccaaga aaccgattcc ttgatgccgc tctcctgggt ttgtacggcc ccgccgttgc
      961 tcagggaggc agaactcttc tgccgcagga aagcccgggc tccacgcagc gccggctgag
     1021 tctgctccag cgtggccttc agcgggtgga acagggacac ggggcccagc tgggacaggc
     1081 ctccgggggc ttgctgagtc tggtctctgc tggttttgtt ctgtttgtag aagtcgaaga
     1141 tcatcagagc tgcatagact ttgcccaccg tcatctcgtc aggcttatgg ggtggtacca
     1201 gtaagtccag ggtcttctgg ggcagattgg cccacacaca ggaaatctcc ttcctcagct
     1261 ctgcgtcgca ctggtgctgc ttggtcccag ctggtgccag cttgatgtcc agcgctgtcc
     1321 gaatgagagc catcagcgtg gatgtgaagt gcaccgtcat gtcctcactg gagatgggca
     1381 tgttcatgcg gaccaggcgc ttgtacgcga ctcgagcagg gcacttcttc cccagcccca
     1441 ggggtgggga catgtgcttc agcatctcaa acatgtcact gtaactgatg cgcccacacg
     1501 cggccgggtc gtattcagcc cagacccgga tgaactcatc caggtggtga ggccctaaga
     1561 tggaagagtc ccgtgtaagg tactcaaaat tgtccatgat cacagccaca aagaggttca
     1621 acatcagaaa ggagcagagg aagatgaagg agacgaagta aaagtaggca aagtcgctgc
     1681 cgcactcgct ggcattggag tgctcgtcgc aggcccggct gctgaggcac gacagcatta
     1741 tctcgtgcca ggcctccccc gtggcgctcc tgaacagcaa catcaaggcc tgcagaaacg
     1801 tccggaagtt attgtgcctg ttgatgctgg tgtcatcgtc cagggcgatg ttcccaaaaa
     1861 cctgcatgcc aatgatggcg tagatgaaga agagcatggc gatgaggaga cacacgtagg
     1921 gcagcgcctt gaaggactgc acgaaggtcc aaagcaggat gcggatggtg tagccctggc
     1981 gcagcagctt gatgagccgc gcggcccgga acaggcgcag gaagctgagg ttgatgaagt
     2041 tgttcgcaat ctctgttacc aaaatatcag taatacttcc caacaccgtg acaaagtcaa
     2101 agacattcca ggcatctctg aaatagttca gcaccccgaa ggcgatgatc ttcagcacac
     2161 actccatgga gaacatggac gtgaacacga tgttcaggca tttcagcatt agctcatact
     2221 cgtacggcgc atcgtagaac ttcatcatca gcaccacagt gttgagggcg atcatagcca
     2281 tgatgaagta ctcaaaaggt ggggacacca cgaacgtcca cgtcttatac tggaacgact
     2341 gcttgttctg gggcatgtac cgcgtcaggg gtctggcgct gatggcaaag tcgatgcaag
     2401 ccctctcgtt cttctccagg ctgcactcgg acatcacctt gtccccctgc tcctggaagg
     2461 tgatgatgat caaggccacg aagatgttga caaagaagaa gggaaagacc acaaagtaga
     2521 ccacgtagaa gatggagagc tccatgcggt acccggggct cgggccctgc tcctcgtagg
     2581 tggcatccac agagtgtttc agcaccatgg gccacccttc ccctgtggac accgtgaaga
     2641 gcgttagcag agcccagagc acgttgtcat agtggaagtc atatttcttc cactgcctcg
     2701 gttgagcttc cacttcctcc ttctcataat ccaaatactg acccctgcag tcacgctcca
     2761 gctccttgga ttcgtccgtg cagtagaaga acttcccttt gaagagctgc acggcaatga
     2821 cggcaaagat gaacatgaag agcatgtaga cgatcaggat gttgaggacg tttttcagtg
     2881 agttcaccac acagtcaaac acggccttga gcttgggcag ccgcttgatg gtcttgagtg
     2941 gccgcagcac acgcaggact ctcagggatt tgatggtgct gatgtctttc cctttggatc
     3001 ctgagaaagc aaatgccacc agggccccac tgaccacaat gaagtccaga atgttccaca
     3061 gatcccggaa ataggctcca gggtgtagca gcagtcccaa atcgatcatc tttatcacca
     3121 tctcgaaggt gaagacgcct gtgaagatgt agtccatgta cttcagagca ttattcctgg
     3181 gagagtctgt tctcacgggg tcctcggcag ccagggcgat gctgctcagg gcgatgacca
     3241 cgaggatgac catctcgaag tatcgcatgg tcacaatgta gtggcagcag cggcgcagca
     3301 ggttggttgg gcttaaacag aacatggaac tgtacgggac aatgggccga gggccgctcc
     3361 tcatcacatc gctggtttcc acctccttct tcccctctgc ttgactttcc aggtccacgt
     3421 ttccactggg cactaccgtg gtctccccgg gggggccggt cagcgtcaca ggaatgtgca
     3481 cagtggtgct ggtgtccggg ggctgactac ccatgcgggt gacgttccgc tggttgtccg
     3541 catcctctgg ctgctcctcc accttctgca gacaggtgct gggcagcgta tgcacagcgc
     3601 ccactcccag catgccgatg gcctccaggt cacagggcag ctccttgggc tggtggttcc
     3661 gagcctcctt gtccttctcg gcctccgtgg cctcgccccc cttctcggca gcctccttgt
     3721 ccttctccgc ggtctcctgg gtgggcggcg ccttgtgccg agcccggtgc cgccgcgcag
     3781 gctcctcgct gctctcagcc tcccgggggc acgcccgggg gcccgtgcgg tgtcgtgcgc
     3841 gccgctcgcc cctggtcccg gacgctgggc cctccttgcc cgggtcgggc ccgtgccggt
     3901 gcgcgcggtg gcggcggggc tccctctcag ctgcctcctc cggtgacccg cgccggtggt
     3961 ggcgccgacc accttcagga cacgggccac ggccgcgatc gctgcgcacc tccggggcgc
     4021 ccggggcctc cttgctgcgg ctgcggcgtg gccgcccccg ctcctcgcgg ggccccagct
     4081 ccccgccctc tgccctcaga gcttcggctc tgtcctgttc ccccgccgag gggaccgtgg
     4141 cgggagcctt gtccttgtct cggtgccggt gatgcctgcg cgggggatcc gcaccctcgg
     4201 gggcctccga gccatccggc cgggacttcc cccccggggg gccccgcgga gcgtcgcggc
     4261 cgggctcaac caccagcggc cggtccaggt gcgtcttcac gtcgggccgc aggtggcgcg
     4321 cggtggcgta gcgcagcctc tcctcggggt ccatctcgct gtacagcgcc tcgcagctgg
     4381 ccctcaggtt ctgcagccgc agctggctgg ctcgctgctc ccacaccgag cgcgctttgg
     4441 ccgagttctg ctgcctggcg gcaatggaga tgttcgcagc agacatgggg ctgacttcag
     4501 ccacttcctt ggccttctgc aaggcgagtt tctgattggc tgcttcttcc atctcctctt
     4561 catccttggt cagctcctga gcattggcaa ggttgtccac agcaatggcc aggaagacat
     4621 tcagcagggt gtagttccca aacagagtga ggacaatgaa gtagaaggag gagaacattc
     4681 ctctgctgac gccaccttgc gactcgatcc cgtggtacat gaccgcattc cagtcctctc
     4741 cggtcaggat ctggaagacg gtgaggatag cagcagggaa ggtgtcaaag ttggtcgttg
     4801 gggtctcgtc tttaaagttg aactggcctc caaacagctg catccccagc agagcaaaga
     4861 caacaatgaa caggaagagc aggaagagga ggctgatgat ggacttcatg gagttgagca
     4921 gagacaccac caggttccgt agggagttcc agtatttggt gaccttgaag attctcagta
     4981 agcgcagagc ccgcagaaca ctgatcccga aggaggtccc tggcttgacg tcgacagatc
     5041 tgtcagggta gggctccagg gagctctcgt agtatctcca tccgtcgacg ccgtcagggt
     5101 agggctccag ggagctctcg tagtatctcc aaccagcagc ccagaccact tcaaagatac
     5161 tgcccacgat gaccccaaag tcaaaacagt tgaaggagga ccggaagtag ctcctgggcc
     5221 ccaggccata catcttcagg gacatctctg tgaggaagag acccaggaaa acaaactctg
     5281 caaagtacaa tgccgtggtg agccgctgcg gctggttgta gtgcaccatg gccacgcaca
     5341 gtgtgttcag tgccaccaca cacagcaccg tccagtagaa gctctgtgcc ttcaccatgc
     5401 gccggatgaa gaagcggaac atcttctcct tcctccggaa gtacgacgag ctctccgtct
     5461 tcccgctttt caggctggca cgggcgaacg gggaaccggg agcacagagg cctgtgagcc
     5521 ggccctcccc ctcctcggcc tggatcaggt cgcttctgct cttcttggca gctgctctct
     5581 tcaacactgc atccagaggg gacttctcct ctgcgttcct gtcctcctca gccagcatga
     5641 cttcctcagc cttgaagatc cactccaggt acccgttgag ctcccgctca atctgctgct
     5701 gccgccggag cttcaggaag gcgcggcggt tctccactct ctcgcgctcc ttagcaaact
     5761 ctcctgagag cacacccagc accaggttga gcatgaagaa ggagccgatg atgatgagag
     5821 ggatgaagta gagccagttc cacgtgttgc cggctgcatc gtttgtattg tagaggatgt
     5881 cagtccagcc ctccatggtg atgcactgga acacggtcaa gatggcaaac aggatgttgt
     5941 caaagttggt gataccaaag ttgggtccgg cccagtactc ccggcactcc gtgtctccct
     6001 cacagagccg ggccggggcc tccttgccac aggggaagtc acccacggga tctgggtctg
     6061 tgctgttggg gaagcaggcc ttgtggaact tgcccatgta gaactcgagg ccgatgatgg
     6121 caaacatgag gatggcgaag aagagcagca gcccgatctg cagcagcggg accatggcct
     6181 tcatgatgga cttgagcacc acctgcaagc tcggaatccc agacaccagc ttcaggggcc
     6241 ttagcacacg cacagccctc agcgttcgca ggtcgaagtc ggttccggct gtggcaagaa
     6301 tccccgtgag gactaccacg aagtccatga cgttccagcc gttccgcaga taggagccct
     6361 tgtgcagaac aaagcccagg gccaggatct tgatccccgc ctcgaagcag aagatcccaa
     6421 tgaagtaggg ctcggtgtca tccaggcgct cggacatagg cgtcttgtcc ccgtcaggga
     6481 ggtgctgctc cagggccagc acgatgcagt tggcgatgat ggtggccagg atcatgtact
     6541 cgaacggagg ccactcggtg atgcgcttgg cgtatttgcg gacgacgttg tcctcgctga
     6601 agacgaagag cgagcggttg acggtgaagc agttctgctt gaccgggatg gggttgtaca
     6661 gcgccatggt ccgcgcgcgc tgcgcgatcg actgtttgta gaggacccgc tggcccggcg
     6721 gcagcccccc ggggcccggg ccgccagccc cgccggcccc gccgccccgg gcccgctccg
     6781 cgccgcccgc gcccccatag cggccgccca gctcgtcccc gaagcggacc atgatcaagc
     6841 ttcgacctcg agggggggcc cggtaccctc gaggaattcc agcacactgg cggccgttac
     6901 tagtggatcc gagctcggta ccaagcttgg gtctccctat agtgagtcgt attaatttcg
     6961 ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc
     7021 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     7081 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     7141 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     7201 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     7261 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     7321 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     7381 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     7441 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     7501 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     7561 taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca
     7621 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
     7681 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
     7741 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga
     7801 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8161 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8461 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9781 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9841 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9901 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9961 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat
    10021 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
    10081 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
    10141 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
    10201 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
    10261 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
    10321 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
    10381 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
    10441 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
    10501 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
    10561 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
    10621 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
    10681 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
    10741 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
    10801 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
    10861 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
    10921 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
    10981 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
    11041 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
    11101 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatccga aaatggatat
    11161 acaagctccc gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
    11221 ctggaatagc tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca
    11281 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
    11341 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
    11401 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
    11461 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat
    11521 taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
    11581 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
    11641 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
    11701 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
    11761 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
    11821 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
    11881 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    11941 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
    12001 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
    12061 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
    12121 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
    12181 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgagct
    12241 ctagcattta ggtgacacta tagaataggg ccctctagat gcatgctcga gcggccgcca
    12301 gtgtgatgga tatctgcaga attcgtcgaa gcttgctaga actagtctag caccggcggt
    12361 cgcggtcggg gtggtggcag ccgcgccgcg cccggccgcc cccgcccagc cccagagtgc
    12421 agtggtagcc gttgggcacg cggcgcagcg gcgggggctg cgaggtcagt gaggacacgt
    12481 acgaggtccg ggaggagcgg cccgagttgc tggccgcggt ctcctcaaag gcgggcgcgt
    12541 cctcgggcag ggcacgggcg ggcgcctcgc tgtccagccg ctgccccagg taggggtcgg
    12601 agccgatgcg cgagccgggc gccaggggcc ggctgagcg
//
