LOCUS       sequence-406780-        4588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mNeonGreen-expression vector for Fusobacterium 
            nucleatum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ponath F, Zhu Y, Cosi V, Vogel J
  TITLE     Expanding the genetic toolkit helps dissect a global stress response
            in the early-branching species Fusobacterium nucleatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 
            10.1073/pnas.2201460119. Epub 2022 Sep 26.
  PUBMED    36161895
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-4"; volume: "119(40):e2201460119. 
            doi"; pages: " 10.1073/pnas.2201460119. Epub 2022 Sep 26"
COMMENT     Sequence Label: pVoPo-mNG
FEATURES             Location/Qualifiers
     source          1..4588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(220..231)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      810..844
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      942..1529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1431..1450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             3284..3292
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 taaatttcgt gaacagaaat aggctttttt ttgatttgct ttcttttcta agtctttcac
       61 aaaatacttc gttatataca cccccacagc ttcattttta acagtttctt taatcttatt
      121 tatttttata aatccattcg cccacaattc cccaagcttc ttattatcta tatatgggac
      181 cgagaacagc accaaatgat aatggattgc tcccctctgc tggagttccc aagttgcaat
      241 atatttaagc tgttgatttt tcaagtacct tttcaatctc ttaataaata aagtaaattc
      301 acgatttgcc ctttctatat cctgtatatt ttctttaaaa gtaagagtta aaaaggtact
      361 tttatcatca taattacaat caattatcct ttttatttca aatcgcttgt cctggtaata
      421 tgctttcctt cgttgcatac tttcagcttt ttcaatgcta cttaattctt caaaatctct
      481 tctttttctt cctggtcttc ttttaaagtc aaaagcagtt aaagcatcaa tattgaaaat
      541 gtattcagtt atattttttg attttatttc tttagtgtta taaaatttca actcaataaa
      601 ttcccccttt attaatctgc ataatctttt ttataatgtc ctttacaacc taatattctt
      661 aatctatcat tttttaagtc ataaattagt ctatgttcat cacttattct tctgctataa
      721 atagccctat ttttaaaatg ttttaatctc tcaggcttac cagtaccgcg gccgctccag
      781 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
      841 ttattggtga gaatccaagc actagtcatg accaaaatcc cttaacgtga gttttcgttc
      901 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      961 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1021 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1081 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1141 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1201 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1261 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1321 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1381 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1441 tggtatcttt tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1501 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1561 tggctaaaaa aaataaaaat attgaaaaaa cttagaaaat ataatagaat taagtagttt
     1621 aataatttaa gggaggagaa taaatggttt ctaaaggaga agaagataat atggcatcat
     1681 tacctgcaac tcatgaatta cacatttttg gttcaataaa tggtgttgat tttgatatgg
     1741 taggacaagg aacaggaaat cctaatgatg gatatgaaga acttaatcta aagagtacta
     1801 aaggagattt acaattttca ccatggattt tagttccaca tataggatat ggatttcatc
     1861 agtatttacc ttatcctgat ggaatgtcac cttttcaagc tgctatggta gatggtagtg
     1921 gatatcaagt tcatagaact atgcaatttg aagatggagc ttctttaact gttaattaca
     1981 gatatacata tgaaggatct catataaaag gagaagcaca agttaaagga acaggttttc
     2041 cagcagatgg tccagttatg acaaattctt taactgcagc agattggtgt agatcaaaaa
     2101 agacatatcc taatgacaaa acaataatta gtacatttaa atggagttat actactggaa
     2161 atggtaaaag atatagatct acagcaagaa ctacatatac ttttgcaaaa ccaatggctg
     2221 ctaattatct taagaatcaa ccaatgtatg tattcagaaa aacagaactt aaacattcta
     2281 aaacagagtt aaacttcaaa gaatggcaaa aagcttttac tgatgttatg ggaatggatg
     2341 aattgtacaa ataaaggatc ctataagttt taataaaact ttaaatagaa aaaggcttct
     2401 ctcatgagaa gtctttttta tttaaaataa atataaaata aaatagaggc tataaatagc
     2461 ctctatttta tgtgagaaat ccctaaataa aaagatgcca gtgtgctgga attcgccctt
     2521 aggctgcgct agggacctct ttagctccct tttgctggcg aattcttatg gattttaggg
     2581 taacaaaaaa caccgtattt ctacgatgtt tttgcttaaa tacttgtttt tagttacaga
     2641 caaacctgaa gttaactatt tatcaattcc tgcaattcgt ttacaaaacg gcaaatgtga
     2701 aatccgtcac atactgcgtg atgaacttga attgccaaag gaagtataat tttgttatct
     2761 tctttataat atttccccat agtaaaaata ggaatcaaat aatcatatcc tttctgcaaa
     2821 ttcagattaa agccatcgaa ggttgaccac ggtatcatag atacattaaa aatgttttcc
     2881 ggagcatttg gctttccttc cattctatga ttgtttccat accgttgcgt atcactttca
     2941 taatctgcta aaaatgattt aaagtcagac ttacactcag tccaaaggct ggaaaatgtt
     3001 tcagtatcat tgtgaaatat tgtatagctt ggtatcatct catcatatat ccccaattca
     3061 ccatcttgat tgattgccgt cctaaactct gaatggcggt ttacaatcat tgcaatataa
     3121 taaagcattg caggatatag tttcattccc ttttccttta tttgtgtgat atccacttta
     3181 acggtcatgc tgtaggtaca aggtacactt gcaaagtagt ggtcaaaata ctcttttctg
     3241 ttccaactat ttttatcaat tttttcaaat accatttaaa attacctcct taaaatatac
     3301 tgctttaatt gtataatttt agctaattat tgtcaagtat ttttaaagtt atgtaaattt
     3361 tttacttata ttttatcgat cgttctagat attctttttt aaacttttca aatgcttcaa
     3421 attctacatc tgcaacttct acatcttttc cagtatttcc aaacttcaca caatttttgt
     3481 aaacatttct taatttttcc aagtattcta tgtcttcctt ttcttcttta acatactctt
     3541 gtaactgtgt catcaaatta gttttgtctt ccacattccc cctttttctt gtaattgcct
     3601 tatttaattc ttcaaatctt attgttttca ttttattgta cctccaattt ttttatatat
     3661 ctatgttaaa aaacatattg acaattgatt aaaacaatga tattatggtc ggttgagctc
     3721 attgagctga gtgcagactt tcccccttgt aaaaagggga aaaaacgatt gataatgata
     3781 caatggtcgt atatcgctat gttcgcatcg tttaacttac tttatcgcat atttaattat
     3841 atgccagtgt tagccctgcc caacacctag agttttagct tactttctaa attataaccc
     3901 taataaagta cccactcaga gcacctccaa gacttttaaa ggtagcctcg caccttgcta
     3961 caattcataa taccaccatt acggtgctat accgtttcga gcagaacggc tatacatacg
     4021 aatttatctt atgtataaaa tttgttacaa atataatagt aagttttttt tatataaaaa
     4081 agcagatcgc ttttattttt tttgcaacct gcttcacaac tcattttttt actttagtaa
     4141 ataaatcctt tacatctttt aaaaaatatg gtatattaag tcaaagcagg cataacatat
     4201 atgttatgtt ttatagtaaa aatggactat tcaactattt aaaaaatggt agaatacaaa
     4261 taattttcct ggcagtcaat tattttttaa aaatttttac tattatattt ttaaatttta
     4321 aattttagat agagttattt ggcgataact ctttttgtct tacttctgag tttagcatat
     4381 tttttttgtt ttgtaaatcc atttctttat ttttctttac aacataaact ttttttactt
     4441 ctctataaat cttctcacca gttgaaacat caatttctaa cagttctcta gcaataaaat
     4501 ttttaatata gagtaaatct ttttcatctg taagtatatc attcatttca tcaaaatcca
     4561 actttttttt ggtttctttt ggttttat
//
