LOCUS       sequence-406782-        4535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mCherry-expression vector for Fusobacterium nucleatum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ponath F, Zhu Y, Cosi V, Vogel J
  TITLE     Expanding the genetic toolkit helps dissect a global stress response
            in the early-branching species Fusobacterium nucleatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2201460119. doi: 
            10.1073/pnas.2201460119. Epub 2022 Sep 26.
  PUBMED    36161895
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-4"; volume: "119(40):e2201460119. 
            doi"; pages: " 10.1073/pnas.2201460119. Epub 2022 Sep 26"
COMMENT     Sequence Label: pVoPo-mCh
FEATURES             Location/Qualifiers
     source          1..4535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      360..394
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      1422..2010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1911..1930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             2781..2789
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4305..4316)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttcaatgcta cttaattctt caaaatctct tctttttctt cctggtcttc ttttaaagtc
       61 aaaagcagtt aaagcatcaa tattgaaaat gtattcagtt atattttttg attttatttc
      121 tttagtgtta taaaatttca actcaataaa ttcccccttt attaatctgc ataatctttt
      181 ttataatgtc ctttacaacc taatattctt aatctatcat tttttaagtc ataaattagt
      241 ctatgttcat cacttattct tctgctataa atagccctat ttttaaaatg ttttaatctc
      301 tcaggcttac cagtaccgcg gccgctccag taatgacctc agaactccat ctggatttgt
      361 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtaagc
      421 ataaaaaaaa gttggataat atactaaaat agtgtataat aaatatgtat attagaaagg
      481 aggaatatat aatggtatct aaaggagaag aagataatat agctataatt aaagaattta
      541 tgagatttaa agttcatatg gaaggatctg ttaatggaca tgaatttgaa atagaaggag
      601 aaggagaagg aagaccttat gaaggtactc aaacagctaa attaaaagta actaaaggtg
      661 gtccattacc atttgcatgg gatatattaa gtcctcaatt tatgtatgga tctaaagcat
      721 atgttaaaca tcctgctgat atacctgatt atttaaaatt atcatttcca gaaggtttta
      781 aatgggaaag agttatgaat tttgaagatg gtggagtagt tactgtaaca caagatagtt
      841 cattacaaga tggagatttt atatataaag ttaaattaag aggtactaat tttccatctg
      901 atggaccagt aatgcaaaag aaaactatgg gatgggaagc atcatctgaa agaatgtatc
      961 cagaagatgg agcattaaaa ggtgaaatta aacaaagatt aaaattaaaa gatggaggtc
     1021 attatgatgc tgaagttaag actacatata aagctaagaa accagttcaa ttaccaggtg
     1081 catataatgt taatattaaa ttagatataa cttctcataa tgaagattat actatagttg
     1141 aacaatatga aagagcagaa ggaagacatt caacaggagg tatggatgaa ttatataaat
     1201 aaggatccta taagttttaa taaaacttta aatagaaaaa ggcttctcta atgagaagtc
     1261 ttttttattt aaaataaata taaaataaaa tagaggctat aaatagcctc tattttatgt
     1321 gagaaatccc taaataaaaa actagtcatg accaaaatcc cttaacgtga gttttcgttc
     1381 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1441 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1501 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1561 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1621 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1681 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1741 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1801 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1861 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1921 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1981 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2041 ctggcctttt gctggcgaat tcttatggat tttagggtaa caaaaaacac cgtatttcta
     2101 cgatgttttt gcttaaatac ttgtttttag ttacagacaa acctgaagtt aactatttat
     2161 caattcctgc aattcgttta caaaacggca aatgtgaaat ccgtcacata ctgcgtgatg
     2221 aacttgaatt gccaaaggaa gtataatttt gttatcttct ttataatatt tccccatagt
     2281 aaaaatagga atcaaataat catatccttt ctgcaaattc agattaaagc catcgaaggt
     2341 tgaccacggt atcatagata cattaaaaat gttttccgga gcatttggct ttccttccat
     2401 tctatgattg tttccatacc gttgcgtatc actttcataa tctgctaaaa atgatttaaa
     2461 gtcagactta cactcagtcc aaaggctgga aaatgtttca gtatcattgt gaaatattgt
     2521 atagcttggt atcatctcat catatatccc caattcacca tcttgattga ttgccgtcct
     2581 aaactctgaa tggcggttta caatcattgc aatataataa agcattgcag gatatagttt
     2641 cattcccttt tcctttattt gtgtgatatc cactttaacg gtcatgctgt aggtacaagg
     2701 tacacttgca aagtagtggt caaaatactc ttttctgttc caactatttt tatcaatttt
     2761 ttcaaatacc atttaaaatt acctccttaa aatatactgc tttaattgta taattttagc
     2821 taattattgt caagtatttt taaagttatg taaatttttt acttatattt tatcgatcgt
     2881 tctagatatt cttttttaaa cttttcaaat gcttcaaatt ctacatctgc aacttctaca
     2941 tcttttccag tatttccaaa cttcacacaa tttttgtaaa catttcttaa tttttccaag
     3001 tattctatgt cttccttttc ttctttaaca tactcttgta actgtgtcat caaattagtt
     3061 ttgtcttcca cattccccct ttttcttgta attgccttat ttaattcttc aaatcttatt
     3121 gttttcattt tattgtacct ccaatttttt tatatatcta tgttaaaaaa catattgaca
     3181 attgattaaa acaatgatat tatggtcggt tgagctcatt gagctgagtg cagactttcc
     3241 cccttgtaaa aaggggaaaa aacgattgat aatgatacaa tggtcgtata tcgctatgtt
     3301 cgcatcgttt aacttacttt atcgcatatt taattatatg ccagtgttag ccctgcccaa
     3361 cacctagagt tttagcttac tttctaaatt ataaccctaa taaagtaccc actcagagca
     3421 cctccaagac ttttaaaggt agcctcgcac cttgctacaa ttcataatac caacattacg
     3481 gtgctatacc gtttcgagca gaacggctat acatacgaat ttatcttatg tataaaattt
     3541 gttacaaata taatagtaag ttttttttat ataaaaaagc agatcgcttt tatttttttt
     3601 gcaacctgct tcacaactca tttttttact ttagtaaata aatcctttac atcttttaaa
     3661 aaatatggta tattaagtca aagcaggcat aacatatatg ttatgtttta tagtaaaaat
     3721 ggactattca actatttaaa aaatggtaga atacaaataa ttttcctggc agtcaattat
     3781 tttttaaaaa tttttactat tatattttta aattttaaat tttagataga gttatttggc
     3841 gataactctt tttgtcttac ttctgagttt agcatatttt ttttgttttg taaatccatt
     3901 tctttatttt tctttacaac ataaactttt tttacttctc tataaatctt ctcaccagtt
     3961 gaaacatcaa tttctaacag ttctctagca ataaaatttt taatatagag taaatctttt
     4021 tcatctgtaa gtatatcatt catttcatca aaatccaact tttttttggt ttcttttggt
     4081 tttattaaat ttcgtgaaca gaaataggct tttttttgat ttgctttctt ttctaagtct
     4141 ttcacaaaat acttcgttat atacaccccc acagcttcat ttttaacagt ttctttaatc
     4201 ttatttattt ttataaatcc attcgcccac aattccccaa gcttcttatt atctatatat
     4261 gggaccgaga acagcaccaa atgataatgg attgctcccc tctgctggag ttcccaagtt
     4321 gcaatatatt taagctgttg atttttcaag taccttttca atctcttaat aaataaagta
     4381 aattcacgat ttgccctttc tatatcctgt atattttctt taaaagtaag agttaaaaag
     4441 gtacttttat catcataatt acaatcaatt atccttttta tttcaaatcg cttgtcctgg
     4501 taatatgctt tccttcgttg catactttca gcttt
//
