LOCUS       sequence-406785-       15039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Violacein pathway. Native promoter and CDS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15039)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 15039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-Violacein-native
FEATURES             Location/Qualifiers
     source          1..15039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5907..5916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(7058..7105)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     7101..7119
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(7172..7189)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(7326..7523)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(7529..8338)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8252..8271
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8369..8712)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    8968..9001
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9004..9022
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9004..9021
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(9023..10840)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     rep_origin      12638..13026
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            13045..13153
                     /note="incP origin of transfer"
     terminator      complement(13300..13339)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(join(13343..14134,14135..14203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14204..14295)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcctcagcg ttgcagcgcg tagcgcaggt tctgcgccag cgcttccggc atcggcggca
       61 gcgacttgcg gcgcgcgtcg aagctctgca cgaactcggc gctggacagg tgcatgcgct
      121 cctccaccgt ctcgaaccag acccggctgt tgtcggcatg gccgcggaac agctggacca
      181 gcggcagcgc gcgctcctcg aagcgcttca gcgcggccgg cacgccgtcc tcggtgcaca
      241 gcgccttcac cagcagctgc gccaccacca ccgccatcgt ggtgccgtgg ccgatggaga
      301 agtggccgga ctgcagcgcg tcgcccagca gcaccagctt gccgtcgtgg cagcggtcgt
      361 ggctcagggt catgaagttg cgccagtcga ggccgggctg gctcaccagg ccgtggccgc
      421 ccagctcggc ctggaacacc ttggcgacgt attcggccga cgcctcttcc gacatctcgc
      481 ccaggcgggc gcgggcatag gtctcctcgc tgcactcgac gatgaaggtg ctcatcgtgt
      541 ccgagtactt gtaggcgtgg gcgatgaaaa tgtccttgcc gtgggtgcgg aacaccaggt
      601 tcatctggtc gaacagctgg ctggtgccgt accagatgta cttgttgcgg ccgtagtcca
      661 cctgcggcac cagcgcctcg gtgaagtggg cggtcttgtg attgacgccg ttggccagca
      721 ccaccaggtc gtagtcggcc agcggcagct cgccatgctc cagcagcggc gattcgaagc
      781 ggatggcgat gccctgcgag cggcacttgt cgcgcaaggc gtgcaccagg ccgcggcgct
      841 ccacgccgca cagcagcacg ccggtgctca tcaggctggg ctcgttgtgg tggaccagct
      901 tgaagtcttc caggaactgc ggattcagcc tctccggcgc gtccaggtag gacagcggat
      961 tggccggatg ctggccgggc cggccgggca gcaccacgcc ccagcccagc acttcctgct
     1021 cgtcgttctt ttcgacgatg tctatcgccc acagcggacg cgcctgtttc agttggctgg
     1081 cgaacaccag gccggccggc cccgcgccga tgaccagaat cttcatcagt tgaccctccc
     1141 tatcttgtac caaacgttct gttttctctg gatctgctgc agtctgtcgt acggctcgga
     1201 cgtgaagtac agcttctccg ccatgtccgg cgggtaaagg ccgggaaact tgctatgcat
     1261 gtagcgggtg tagcgggccc gcatgaagaa gatgggattg gagcagctga gcacgtcgta
     1321 gttggcgcgc gccatgtcct gcatcgcgtc ggcctgcacc ttgcgcagct cggtgaactc
     1381 gggaaaggcc ttgtcctggt cgccctggtg gcggtccagc agctcgacga aggtgcgcgc
     1441 gtcctccagc gccatgttca tgccctggcc gaggaaaggc gcggtggcgt gggcggcgtc
     1501 gcccagcagc agcacattgc ccttgtagtg gaaggtgctg gaacggacgt tgatcaggtc
     1561 gttgctgggc ttggccagga actggcgcag catctcgtcg cgcgcgtccc gcggcaggcc
     1621 gccgaagtaa cggtcgaaaa aggcgcgcat cgtcggctcg tcggtggtgg tcaggctggg
     1681 gctgccgctg tacggcaggc agaccgcgat gctgacgctg ccgtccggga tggtggcggc
     1741 gcggccggcg aacaggccgc cggagtccat gccgaagaaa tacagcgtgt ccttgcggta
     1801 gcccagcgcc tgcgcgtccg gcagcaccag cgtcttgtag ccgtggcgga agaaagtctg
     1861 ctggaattcg aagcggcgca acccgctctg catcgcctgc cgcaccgccg agtgcgcgcc
     1921 gtcggcgccg atgatcatat cgccctgcaa gcgctgcggc tggccgtcct tgccctggat
     1981 cagcaccgac ttgccgtcca gatccacgtc caggcacttg tgctcgaagt agtagcggac
     2041 gccggccagg ttggcgtact tgttcagcag cttctgaaac gccgcgcggt tcagcgacag
     2101 cgggcggaaa tcctccagcg gcttgagctc ccgcatccgg tactggccgc cgacggaaaa
     2161 cgccatcgcc acgatgggtt cgccgcaggc gtccagctcc gcgcgcggaa tgccggccgc
     2221 cagcaccgac ttgatgccgc gcacggtcat gctgacgcct atcgcccgcg agctgaccac
     2281 atccacgtag gaagacaggt cccgcagcgg gtcgccgcgc ttttccacca cgtggacctc
     2341 gtagccgcgc ttcgccaggt agatggcggt cagcccgccg gcgagcccgc ctccgactat
     2401 gattgctctt ttcatttcag gcctctctag aaagctttcc acaagccaaa tccagcatct
     2461 gccggcggta gaaatccagc agttccattt gcgcgtcggg cagccagccc ggctccagca
     2521 tcgacgcctg ctccagcagg cgctcgcagc gccgcgccag catccggcag cccagcgcgt
     2581 agtcgtcgta gctgcgggta tccaccggcc ccggcagcgg cggcccggcg gtgcgtccgg
     2641 cgatgcccga cggcaggttc atcagcgcgc aggacagcgg cctgagcagg ccggtcatca
     2701 ggtcgatcgc cgcgttcatc agccgcgagc gcctgaggct gcccagcggc ttgaccgcga
     2761 agtgctgcgc catcatcgcg aacatcagct cgtacacgcc ctggtacagc gccatcagcg
     2821 cgcgcgcccg tccgtccgcc acgcgctggc agcccggcga ctcgtccagc accgggttgc
     2881 gcagcgccgg caacgccggc tcgaacggcg cggactgcgc catgatcctg gccgacatct
     2941 cccgcagccg ctggaaatgc gaatgctcgt aatgcggcga gtccagcgcc ccgccctcgc
     3001 cctggtcggt gacgaaggcg atgccgaaca gcgcgctgtc gcgatcgaac acctccagct
     3061 ggtagccggg atgggcgcgg ttggtcagct cgttgaggaa caagtggtgc tcgccgccgc
     3121 gcttgccggc ctcgccgccg aacaggtcgg gcagatcgag aaaggcctgg cggatggcgg
     3181 cgtagcagtc ggcgatggat ttgcccggcg cagggatgaa gtgcggccat tccagccgga
     3241 cgaagcgcgc cagcgtcgac tcggagaacg gctccagcgc gaattcggtg tccaggccga
     3301 acgcctgccg cgccgcctcg cccatcagcg gcacgccggc gtagaacggc tcgcccagcg
     3361 ccatcagcag gttgttgacc accaggtaat ggatcatctc ctcgtgggcg atctccagca
     3421 gcgcggcgcg gatgccgccg tcgcggcgcc ggtcgccgct gccgcaggcc agctgcagct
     3481 gctccgccgt ccacgcgccg tcgcgcaccc gctgctggcc ctgggcgtaa ttgggaatgg
     3541 aataggcggc gtacaggtac tgcagcatca ccgacaactc cagatccacc gccttgcgca
     3601 gctcggccgc cagctgggcc ttgctctcga tgcgcgccgg cccggccggc ggcggcgcct
     3661 gcagcctggc ctggccctcg acatgggcca ggtatttgag gaacagcctg gccttgggcg
     3721 ccgacagctc gcgggtgctg ggcatgtagt agctcttgtt ccggttctgc ggatcgcaca
     3781 tctgccacat cagcctggcg taggtctcgc acttgcagcg gtcggccagg ctgaacacct
     3841 tgtcggacat gaacgggtag accagctcgt aataggccat cacgtgccgg tacaggaagg
     3901 cgtaatcgac ctcctccacc gtcgcgtcgt ccaacgccca gtcgtccggc agcacccgca
     3961 gcgggatcgc ctccgcgccg tcgtcgagca cgatgcgcgc cgggccgggc cgcaggccgg
     4021 tcagccgcca ttcggcggca tcgccgtcct ggcgcgactc cacgccgacc agacccatcc
     4081 cctcgatccg gtggggaatg tccgggcgcg cgcgggcctc gccgcggaaa tagctgcgca
     4141 gcgcgatgct ttccggaaag aaacggccgt ggcggcggtc cggcgcttcc aaataaagat
     4201 tggaggcgtc ggactgcgtg acccagtcct gctcgcgcca ttcggccagc tcgctggaca
     4261 gcgtcagcga gcccctgggc tcgcgcagca gcggcaggtc gacgatgccg tggttcgtcc
     4321 agtaatcctg gtaaagcgcc tgcggcaccc gcgccagcaa cgcgccgtcc tcgtcgcgca
     4381 gcagcaggtc gcccaacggc agcttggccc cgagatcggg cgtcagcctg tccggcgcgg
     4441 acggctgcgc cgcccgggtg gagaacggaa tggcgcaggc cagattcagc gccacgcggc
     4501 cgccgcttac gcgcagcgtc agatcgccca gcccgggctg gcgcggacgc agcagccggc
     4561 cggccgggta gctggccagt tcgccgcgcc gccacaggcc gaccacgccg accatgtcgt
     4621 ggaacaccgg cgagttgggt tgcggcggcg tcgacatatt gaacagcgcg tactgcaccg
     4681 tcaggccgag cacgtcgtcg tcctccagcg ccagctgcag cctgcgccag gcttcggaat
     4741 cgaatggccc cgggtggaac aggaagtgcg gatggtcttt gggcaccgag aactggaaca
     4801 gccgcgccag gccgaactcc tcgtccagga aatggccgcc gcgctcggcg atgtggccgc
     4861 cgcgcgtcca gcgcgcgccg tggctgtcgt cgatgtcggc ggtgaacagc cagggcgtgc
     4921 ccggtccggc gccggccggg ctgatcgtga actgcccggc gtagatctgc gccgcgtcgc
     4981 ggcgggtggg gtcgctgtcc acccagcgcg cgcggttgaa ggtggtgcgc aggtaatcgt
     5041 tgtaatgccc ccacagcgcc agcctggcgc cgaccaggcc gtcgccgcgg tccgcttcgc
     5101 cgccatccca ctgcacgtgg ctgacggtgg cgctctccca ggagaaatgg ttgttgccgg
     5161 ccgcgttgta gccctcggcc aggctgaacg gcccttccgg gtcagcccgg ccgtccaggc
     5221 cgaaacgcgg ccccagcgac cgcaggtggc ggtggaactc ggtcggatgg cgcgcgaggt
     5281 cgaacggttc gcctgccatg gccaccgtat tgctggccat gtcgatgtgg ccgtgcggat
     5341 cgcggttggc ggtgggcgcg ttgacccgcg cccagccgcg gaaatggatg cgtggaaaat
     5401 ccagaatgct catgtgtttc ccgaacggtc atggctgaca agcgccgccc ggaaccgggc
     5461 ggcgacgcga tgcggcggcc ggaccgttca cgcggcgatg cgctgcagca gcagacggct
     5521 ggcttcgcgg gcgctgagca ggccgccctc catccagccg cagtgctcgg tgtaggcgtc
     5581 cgaacaggcg atgatgccgc tgtcgcggtg gctgagcgcg gacggatggt cgatatcgct
     5641 gtcgcggcag aactccacgc catgcgccca atacttgtgc acatgggcga ggggctgggg
     5701 aatgcgctcg cgaacgatgc ccagcgcgct ggccagatgg gtgcggatct gctccaggta
     5761 gccgtcctct ccttcggcca cgcagccgcg ccagtaattg gccatctcgc tgtcggtgta
     5821 gaagaacagg tacttgtcgc ccttgaagta gatcttgcgc agcgggttgt cgacgatcag
     5881 cacctggtcg tccagcttgt agtccagcca ccatggctcg ccgtaggtga ggaaaccctt
     5941 gaacagcggc agcgagccgt agcgcgcgcc gctccacgcc tcggggaagt cgacattgag
     6001 cccggccatc gccgacggag gaatggccag gatcagatgg cgggtccggt gttccagctt
     6061 ccagccgtcg tcgccggcca gttgcagcag gtagccgtcg ccgtccgtcc tcaccgacag
     6121 cagccggtaa cccaggctga agcgcgcgcc ggcagccttg accttggcct tgatgccctg
     6181 gatcaggccc gcaaagcccg tttccgccgc gaaccactgg ttggcgtcgt tatcggtcac
     6241 gctctggatt tccgggtgct tgccgacgat gtcgtaggcc atctcggccg agatgtcggg
     6301 caggaacagc gcgtcgtagc ccatggagcg gatcatgccc accgcgctgt catggccctg
     6361 gtagcggctg acgaactgga gaaaggattc cttgccatgc tctttcagcc tgggcgacaa
     6421 ctcgttcatc gcccgcttca gcttctgctg gacatggctc ttgaatttca gctgggtgaa
     6481 cggatacacc tcgctcttct ggctgtaatg ctgcatcgcg ctctggaaat gcgggtgcag
     6541 ctgcggggag tatcgccccg cgcccagctc tatcgacgcc ttgccatcca gcatcttcga
     6601 gcggatgcgg ccgcccgcct cctgctgcat gtcgaagatg cgcagcgaca ggccgcggca
     6661 agcgggcgag tcgagcagat ggctggcgca ggtcaggccg ctgatgccgg cgccgacaat
     6721 gcagatatcg gaagaatgct tcatcgcgaa tgtccttttg actagtcaac tcacgcccgc
     6781 cgcgcggcaa cggcggacgg gcatgggcgg cgaaaaccta cggcactcgc gccttggaat
     6841 cgggatgctg cctttcgttt cctcgtcatg cccggtcaat ccgggcacaa aaaatcgttt
     6901 gacattttat tttaacaata aaatgctttt gtaatttcaa gccacattag ttcaaccgga
     6961 ctcattcatc aaccatattg tctgacaatg tggctaatac tgttccaagg gtcagggcat
     7021 gacgtggaga attggataca aaagtgatgg ccttcagcaa aaaacccctc aagacccgtt
     7081 tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt
     7141 cctttcgggc tttgttagca gccggatccc agtggtggtg gtggtggtgc tcgagtgcgg
     7201 ccgcagcgac aacaacataa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     7261 gaagcttctt tcagcaaaaa accccgcgag atccccgaaa gaggccccgc ggggttatgc
     7321 tagagcagta tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca
     7381 cttaacttcg catctggaca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca
     7441 tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa
     7501 acaagaatct ttttattgtc agtactgatt agaaaaactc atcgagcatc aaatgaaact
     7561 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     7621 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     7681 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     7741 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     7801 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcacat
     7861 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     7921 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     7981 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttgccgg
     8041 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     8101 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     8161 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     8221 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     8281 cagcatccat gttggaattt aatcgcggcc tcgaaacgtg agtcttttcc ttacccatgt
     8341 aatcactcct tcttaattac aaatttttgg ttgtttatgt tcggatgtga tgtgagaact
     8401 gtatcctagc aagattttaa aaggaagtat atgaaagaag aacctcagtg gcaaatccta
     8461 accttttata tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt ctgtgagggg
     8521 agcgtttccc tgctcgcagg tctgcagcga ggtgccgtaa tttttgcttc gcgccgtgcg
     8581 gccatcaaaa tgtatggatg caaatgatta tacatgggga tgtatgggct aaatgtacgg
     8641 gcgacagtca catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga gggtattctg
     8701 ggcctccatg tcttgcagat gagcaaacat ataaccgagg aacaaaggta tgcaatttct
     8761 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     8821 actgttcctt gcattctaaa accttaaata cagaaaacag ccttttcaaa gttgttttca
     8881 aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt atgatagaat
     8941 ttgacgtatt accctgttat ccctatacgc gcccggggag cccacgggca cgccctggca
     9001 cccatttagg tgacactata gattacgccg ctacgtcttc cgtgccgtcc tgggcgtcgt
     9061 cttcgtcgtc gtcggtcggc ggcttcgccc acgtgatcga agcgcgcttc tcgatgggcg
     9121 ttccctgccc cctgcccgta gtcgacttcg tgacaacgat cttgtctacg aagagcccga
     9181 cgaacacgcg cttgtcgtct actgacgcgc gcccccacca cgacttaggg ccggtcgggt
     9241 cagcgtcggc gtcttcgggg aaccattggt caaggggaag cttcggggct tcggcggctt
     9301 caagttcggc aagccgctct tccgcccctt gctgccggag cgtcagcgct gcctgttgct
     9361 tccggaagtg cttcctgcca acgggtccgt cgtacgcgcc tgccgcgcgg tcttcgtaca
     9421 gctcttcaag ggcgttcagg gcgtcggcgc gctccgcaac aaggttcgcc cgttcgccgc
     9481 tcttctcagg cgcctcagtg agcttgccga agcgtcgggc ggcttcccac agaagcgcca
     9541 acgtctcttc gtcgccttcg gcgtgcctga tcttgttgaa gatgcgttcc gcaacgaact
     9601 tgtcgagtgc cgccatgctg acgttgcacg tgccttcgtg ctgcccaggt gcggacgggt
     9661 cgaccacctt ccggcgacgg cagcggtaag agtccttgat cgattcttcc ccgcgcttcg
     9721 aagtcatgac ggcgccacac tcgcagtaca gcttgtccat ggcggacaga atggcttgcc
     9781 cccgggaaag ccccttgccg cgccccctgc cgtccaacca cgcctgaagc tcataccact
     9841 cagcgggctc gatgatcggt ccgcaatcaa gctcgaccgg ccggagcgtg atcgggtcgc
     9901 gctgaatgcg gtaaccctca atcttcgtgg tcggcgtgcc gtccggcttc ttcttgtaga
     9961 tcacctcagc ggcgaagccc gcaatacgcg ggtcccgaag gattcgcata acggttgccg
    10021 ggtcccaggc gcttgaagcg gtcttcttcc caatcgtctc gccccgggtc ggcacggcgt
    10081 cagcgtccat gcgcttacaa agccccgtga tgctgcccgg gtgaatggcg gcttgactgc
    10141 ccggcttgaa gggaaggtgt ttgtgcgtct tgatctcacg ccaccaccac cggattacgt
    10201 cgggctcgaa ctcgaagggt ccggtaaggg gagtggtcga gtgcgcaagc ttgttgatga
    10261 cgacattgac cattcggccg ttgcgcgtga tctccttcgt ctccgaaaca agctcgaagc
    10321 cgtaaggcgc cttcccgccg acgtacccgc ccaattcgcg ctgaaggttc ttcgtgtcga
    10381 gaatcttcgc cgacttcagc gaagattctt tgtgcgacgc gtcgagccgc ataatcaggt
    10441 gaatcaggtc catgacgttt ccctgccgga agacgccttc ctgagtggaa acaatcgtca
    10501 cgcccagggc gagcaattcc gagacaatcg gaatcgcgtc catgaccttc aggcgcgaga
    10561 agcgcgacac gtcatagaca atgatcatgt tgagccgccc ggcgcggcat tcgttcagga
    10621 tgcgttcgaa ctccgggcgc tccgccgtcc cgaacgccga cgtgcccggc gcttcgctga
    10681 aatgcccgac gaacctgaac cggcccccgt cgcgctcgac ttcgcgctga aggtcggccg
    10741 ccttgtcttc gttggcgcta cgctgtgtcg ctgggcttgc tgcgctcgaa ttttcgcgct
    10801 cgcgcgactg acggtcgtaa gcacccgcgt acgtgtccat gattaatcct cctttttgtt
    10861 tgatttaaca cttctctttg taatacggtc cacaaaggta tgaataataa tgtggcgcgc
    10921 aaatttgatg caaccattat caccgccaga ggtaaaatag tcaacacgca cggtgttaga
    10981 tatttatccc ttgcggtgat agatttaacg ttccgattta gtacctccat ataaaggagg
    11041 atcaaaatgt caacgaagaa aaagccgctt acacaagagc agctagagga cgcacgtcgt
    11101 ctgaaagcaa tctatgagaa gaaaaagaat gagctgggtc tgtctcagga aagcgtagcc
    11161 gacaagatgg gcatgggtca gagcggcgtt ggcgctctgt ttaacggtat taatgcgctg
    11221 aacgcgtaca acgccgcact gctgaccaag attctgaaag tttccgtcga ggagttctct
    11281 ccttctatag ctcgtgaaat ctatgaaatg tatgaagcgg ttagcatgca accgtctctg
    11341 cgctctgaat acgaataccc ggtcttcagc cacgttcaag caggtatgtt tagcccggaa
    11401 ctgcgtacct tcaccaaagg tgacgctgag cgttgggtat cgactaccaa aaaagcgagc
    11461 gatagcgcgt tttggctgga agtagaaggc aacagcatga cggccccgac gggcagcaag
    11521 ccgtcatttc cggatggtat gctgatcctg gttgatcctg agcaggcggt tgagccggga
    11581 gacttttgca ttgcgcgcct gggtggtgat gaattcacct ttaagaagct gatccgcgac
    11641 tctggccaag ttttcctgca gccgctgaat ccgcaatacc caatgatccc gtgcaacgaa
    11701 tcctgtagcg ttgttggtaa ggtcattgca tcccagtggc cggaagaaac cttcggttaa
    11761 tttgtcagtt acggcaagat gcggcaaaac cccgccgaag cggggttttc ggcgtggatg
    11821 gctttcttgc cgctgtcgct cagctggagc tagtcctgcg gtcacgtagc tctggtgcta
    11881 gcagcctgac tggaattcgg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa
    11941 cattctcttc aacaagttta attccattgc ggtgaaatgg taaaagtcaa ccccctgcga
    12001 tgtatatttt cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct
    12061 tgttatttta cagattttat gtttagatct tttatgcttg cttttcaaaa ggcttgcagg
    12121 caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt
    12181 ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt
    12241 acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt
    12301 ccaccagcta acataaaatg taagccggca ttttggccgc tcctaggtag tgcttttttt
    12361 ccaaggacaa aacagtttct ttttcttgag caggttttat gtttcggtaa tcataaacaa
    12421 taaataaatt atttcattta tgtttaaaaa taaaaaataa aaaagtattt taaattttta
    12481 aaaaagttga ttataagcat gtgacctttt gcaagcaatt aaattttgca atttgtgatt
    12541 taggcaaaag ttactatttc tggctcgtga attctgctct gagccgtcac gctgagtgag
    12601 tgctaggctc cactgtgact gacctgggcg cgccccatgt cagccgttaa gtgttcctgt
    12661 gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca tccctggctt
    12721 gttgtccaca accgttaaac cttaaaggct ttaaaagcct tatatattct tttttttctt
    12781 ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca
    12841 aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt
    12901 acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag
    12961 cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac tgctgatctt
    13021 cagatcggcg cgccggccgg cctacggcca gcctcgcaga gcaggattcc cgttgagcac
    13081 cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc
    13141 acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg
    13201 gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac
    13261 cccgaagcag ggttatgcag cggaaaagga caagggtcgt ccaaaaaaaa aggctccaaa
    13321 aggagccttt aattgtatcg gtttaccaat gtttaatcag gctcgcgcca atttccgcaa
    13381 tctgacggtt acgttcatcc atcgtcgcct ggctgccggt ggtataaatc accacaatac
    13441 ggctcggttt accatccggg cccagcgccg caataatgcc acggctgcca cgttcgcccg
    13501 caccgctttt atccgcaata aaccagccag ccggcagcgc gctacgcagc agcggacccg
    13561 ccactttatc cgcttccatc caatcaatca gttgctggcg gcttgccagg gtcagcagct
    13621 cacccgtcag cagtttacgc agggtggtcg ccattgctgc cggcatggtg gtatcacgtt
    13681 catcgttcgg aatcgcttcg ttcagttccg gttcccaacg atccagacgg gtcacatgat
    13741 cgcccatgtt atgcagaaac gcggtcagtt ctttcggacc gccaatggtc gtcagcagca
    13801 ggttcgccgc ggtgttatcg ctcatggtaa tcgccgcgct gcacagttca cgcacggtca
    13861 tgccatcggt cagatgtttt tcggtcaccg ggctatattc caccagatcg ttctggctat
    13921 aatgaatacg acggcccagt tgttcctggc ccgcatccac acggctcaga accgcaccgc
    13981 acagcagcac tttaaaggtg ctcatcatcg gaaaacgttc ttccggacga aaagattcca
    14041 gaattttgcc gctgttcaga tccagttcaa tatagcccac gcgcgcaccc agttgatctt
    14101 ccgcatcttt cactttcacc agggtttccg gatgcgcaaa caccggcagg caaaacgccg
    14161 caaaaaacgg aatcagcgcc acacgaaaat gctgaatgct catactcttc ctttttcaat
    14221 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    14281 agaaaaataa acaaataggg gaattaaaaa aaagcccgct cattaggcgg gctactaatt
    14341 taaatcgatt atacgcgccc ggggagccct ggggcacgcc ctggcacccc tcggtaccaa
    14401 attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc ggcctcacct
    14461 agcgcttggc ggcgaagacg gcgtcgggaa tctcggcctc gctgacgttg gggaaatcgc
    14521 gcagcgacgc gcgcgacgcc tcgtccccgg tcaccatcct cagcggcgta ccgctggcgg
    14581 cgtccaggta caacgtggac ggccccttgc ccggacgctg gtagcgccag ccgtcggctt
    14641 ccctgcccag caccacgcgg cggccgatat ggcgggcgcc cagccggcgc agcacgtcgc
    14701 gcggcaggaa cagctcggcg aacgggccgg tctcgtcgtc cagcggccgc tcgcacagct
    14761 gctcgcccag ggcggtccgc tcgcggccat aggccacctt ctgcttccag gtgcggccgc
    14821 tggcggcgtt gccgacctcg gacatccaca gccggtagcc ggtgtcgcgc tccgaccagg
    14881 gattgaacag gccgtctatc cgacagatgt cgcgctcgta gtcgaaccag cagtagccgg
    14941 acgtcagctg gtcatccggc agcatcggac tccagtacga cacataggcg ctgctccagc
    15001 gcgccggcag cagcggcggt tcccggtttt ccatgcggc
//
