LOCUS       sequence-406790-       14856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Violacein pathway. pT7-native CDS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-Violacein-pT7native
FEATURES             Location/Qualifiers
     source          1..14856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(927..946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(928..946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(965..1012)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1008..1026
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1079..1096)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1233..1430)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(1436..2245)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2159..2178
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2276..2619)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    2875..2908
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        2911..2929
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2911..2928
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(2930..4747)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     rep_origin      6545..6933
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            6952..7060
                     /note="incP origin of transfer"
     terminator      complement(7207..7246)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(join(7250..8041,8042..8110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8111..8202)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctggtcgt ccagcttgta gtccagccac catggctcgc cgtaggtgag gaaacccttg
       61 aacagcggca gcgagccgta gcgcgcgccg ctccacgcct cggggaagtc gacattgagc
      121 ccggccatcg ccgacggagg aatggccagg atcagatggc gggtccggtg ttccagcttc
      181 cagccgtcgt cgccggccag ttgcagcagg tagccgtcgc cgtccgtcct caccgacagc
      241 agccggtaac ccaggctgaa gcgcgcgccg gcagccttga ccttggcctt gatgccctgg
      301 atcaggcccg caaagcccgt ttccgccgcg aaccactggt tggcgtcgtt atcggtcacg
      361 ctctggattt ccgggtgctt gccgacgatg tcgtaggcca tctcggccga gatgtcgggc
      421 aggaacagcg cgtcgtagcc catggagcgg atcatgccca ccgcgctgtc atggccctgg
      481 tagcggctga cgaactggag aaaggattcc ttgccatgct ctttcagcct gggcgacaac
      541 tcgttcatcg cccgcttcag cttctgctgg acatggctct tgaatttcag ctgggtgaac
      601 ggatacacct cgctcttctg gctgtaatgc tgcatcgcgc tctggaaatg cgggtgcagc
      661 tgcggggagt atcgccccgc gcccagctct atcgacgcct tgccatccag catcttcgag
      721 cggatgcggc cgcccgcctc ctgctgcatg tcgaagatgc gcagcgacag gccgcggcaa
      781 gcgggcgagt cgagcagatg gctggcgcag gtcaggccgc tgatgccggc gccgacaatg
      841 cagatatcgg aagaatgctt catcgcgaat gtccttttga ctagtcaact cacgcccggt
      901 gtccatcaca ttggcctgca ggatctccct atagtgagtc gtattaggcc atgatggcct
      961 tcagcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct agttattgct
     1021 cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatcccagt
     1081 ggtggtggtg gtggtgctcg agtgcggccg cagcgacaac aacataacga aaggcccagt
     1141 ctttcgactg agcctttcgt tttatttgaa gcttctttca gcaaaaaacc ccgcgagatc
     1201 cccgaaagag gccccgcggg gttatgctag agcagtatag cgaccagcat tcacatacga
     1261 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctggacagat gatgtcgagg
     1321 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
     1381 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgattaga
     1441 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     1501 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     1561 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     1621 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     1681 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     1741 tacgctcgtc atcaaaatca ctcacatcaa ccaaaccgtt attcattcgt gattgcgcct
     1801 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     1861 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     1921 ctaatacctg gaatgctgtt ttgccgggga tcgcagtggt gagtaaccat gcatcatcag
     1981 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     2041 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     2101 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     2161 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     2221 aaacgtgagt cttttcctta cccatgtaat cactccttct taattacaaa tttttggttg
     2281 tttatgttcg gatgtgatgt gagaactgta tcctagcaag attttaaaag gaagtatatg
     2341 aaagaagaac ctcagtggca aatcctaacc ttttatattt ctctacaggg gcgcggcgtg
     2401 gggacaattc aacgcgtctg tgaggggagc gtttccctgc tcgcaggtct gcagcgaggt
     2461 gccgtaattt ttgcttcgcg ccgtgcggcc atcaaaatgt atggatgcaa atgattatac
     2521 atggggatgt atgggctaaa tgtacgggcg acagtcacat catgcccctg agctgcgcac
     2581 gtcaagactg tcaaggaggg tattctgggc ctccatgtct tgcagatgag caaacatata
     2641 accgaggaac aaaggtatgc aatttctttt tctacagtat ttaaagatac cccaagaagc
     2701 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacag
     2761 aaaacagcct tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     2821 attttgaaac cacaattatg atagaatttg acgtattacc ctgttatccc tatacgcgcc
     2881 cggggagccc acgggcacgc cctggcaccc atttaggtga cactatagat tacgccgcta
     2941 cgtcttccgt gccgtcctgg gcgtcgtctt cgtcgtcgtc ggtcggcggc ttcgcccacg
     3001 tgatcgaagc gcgcttctcg atgggcgttc cctgccccct gcccgtagtc gacttcgtga
     3061 caacgatctt gtctacgaag agcccgacga acacgcgctt gtcgtctact gacgcgcgcc
     3121 cccaccacga cttagggccg gtcgggtcag cgtcggcgtc ttcggggaac cattggtcaa
     3181 ggggaagctt cggggcttcg gcggcttcaa gttcggcaag ccgctcttcc gccccttgct
     3241 gccggagcgt cagcgctgcc tgttgcttcc ggaagtgctt cctgccaacg ggtccgtcgt
     3301 acgcgcctgc cgcgcggtct tcgtacagct cttcaagggc gttcagggcg tcggcgcgct
     3361 ccgcaacaag gttcgcccgt tcgccgctct tctcaggcgc ctcagtgagc ttgccgaagc
     3421 gtcgggcggc ttcccacaga agcgccaacg tctcttcgtc gccttcggcg tgcctgatct
     3481 tgttgaagat gcgttccgca acgaacttgt cgagtgccgc catgctgacg ttgcacgtgc
     3541 cttcgtgctg cccaggtgcg gacgggtcga ccaccttccg gcgacggcag cggtaagagt
     3601 ccttgatcga ttcttccccg cgcttcgaag tcatgacggc gccacactcg cagtacagct
     3661 tgtccatggc ggacagaatg gcttgccccc gggaaagccc cttgccgcgc cccctgccgt
     3721 ccaaccacgc ctgaagctca taccactcag cgggctcgat gatcggtccg caatcaagct
     3781 cgaccggccg gagcgtgatc gggtcgcgct gaatgcggta accctcaatc ttcgtggtcg
     3841 gcgtgccgtc cggcttcttc ttgtagatca cctcagcggc gaagcccgca atacgcgggt
     3901 cccgaaggat tcgcataacg gttgccgggt cccaggcgct tgaagcggtc ttcttcccaa
     3961 tcgtctcgcc ccgggtcggc acggcgtcag cgtccatgcg cttacaaagc cccgtgatgc
     4021 tgcccgggtg aatggcggct tgactgcccg gcttgaaggg aaggtgtttg tgcgtcttga
     4081 tctcacgcca ccaccaccgg attacgtcgg gctcgaactc gaagggtccg gtaaggggag
     4141 tggtcgagtg cgcaagcttg ttgatgacga cattgaccat tcggccgttg cgcgtgatct
     4201 ccttcgtctc cgaaacaagc tcgaagccgt aaggcgcctt cccgccgacg tacccgccca
     4261 attcgcgctg aaggttcttc gtgtcgagaa tcttcgccga cttcagcgaa gattctttgt
     4321 gcgacgcgtc gagccgcata atcaggtgaa tcaggtccat gacgtttccc tgccggaaga
     4381 cgccttcctg agtggaaaca atcgtcacgc ccagggcgag caattccgag acaatcggaa
     4441 tcgcgtccat gaccttcagg cgcgagaagc gcgacacgtc atagacaatg atcatgttga
     4501 gccgcccggc gcggcattcg ttcaggatgc gttcgaactc cgggcgctcc gccgtcccga
     4561 acgccgacgt gcccggcgct tcgctgaaat gcccgacgaa cctgaaccgg cccccgtcgc
     4621 gctcgacttc gcgctgaagg tcggccgcct tgtcttcgtt ggcgctacgc tgtgtcgctg
     4681 ggcttgctgc gctcgaattt tcgcgctcgc gcgactgacg gtcgtaagca cccgcgtacg
     4741 tgtccatgat taatcctcct ttttgtttga tttaacactt ctctttgtaa tacggtccac
     4801 aaaggtatga ataataatgt ggcgcgcaaa tttgatgcaa ccattatcac cgccagaggt
     4861 aaaatagtca acacgcacgg tgttagatat ttatcccttg cggtgataga tttaacgttc
     4921 cgatttagta cctccatata aaggaggatc aaaatgtcaa cgaagaaaaa gccgcttaca
     4981 caagagcagc tagaggacgc acgtcgtctg aaagcaatct atgagaagaa aaagaatgag
     5041 ctgggtctgt ctcaggaaag cgtagccgac aagatgggca tgggtcagag cggcgttggc
     5101 gctctgttta acggtattaa tgcgctgaac gcgtacaacg ccgcactgct gaccaagatt
     5161 ctgaaagttt ccgtcgagga gttctctcct tctatagctc gtgaaatcta tgaaatgtat
     5221 gaagcggtta gcatgcaacc gtctctgcgc tctgaatacg aatacccggt cttcagccac
     5281 gttcaagcag gtatgtttag cccggaactg cgtaccttca ccaaaggtga cgctgagcgt
     5341 tgggtatcga ctaccaaaaa agcgagcgat agcgcgtttt ggctggaagt agaaggcaac
     5401 agcatgacgg ccccgacggg cagcaagccg tcatttccgg atggtatgct gatcctggtt
     5461 gatcctgagc aggcggttga gccgggagac ttttgcattg cgcgcctggg tggtgatgaa
     5521 ttcaccttta agaagctgat ccgcgactct ggccaagttt tcctgcagcc gctgaatccg
     5581 caatacccaa tgatcccgtg caacgaatcc tgtagcgttg ttggtaaggt cattgcatcc
     5641 cagtggccgg aagaaacctt cggttaattt gtcagttacg gcaagatgcg gcaaaacccc
     5701 gccgaagcgg ggttttcggc gtggatggct ttcttgccgc tgtcgctcag ctggagctag
     5761 tcctgcggtc acgtagctct ggtgctagca gcctgactgg aattcggcta gcaagaatcg
     5821 ggtcattgta gcgtatgcgc ctgtgaacat tctcttcaac aagtttaatt ccattgcggt
     5881 gaaatggtaa aagtcaaccc cctgcgatgt atattttcct gtacaatcaa tcaaaaagcc
     5941 aaatgattta gcattatctt tacatcttgt tattttacag attttatgtt tagatctttt
     6001 atgcttgctt ttcaaaaggc ttgcaggcaa gtgcacaaac aatacttaaa taaatactac
     6061 tcagtaataa cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt
     6121 acaccatttg tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc
     6181 gcatcaccaa cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gccggcattt
     6241 tggccgctcc taggtagtgc tttttttcca aggacaaaac agtttctttt tcttgagcag
     6301 gttttatgtt tcggtaatca taaacaataa ataaattatt tcatttatgt ttaaaaataa
     6361 aaaataaaaa agtattttaa atttttaaaa aagttgatta taagcatgtg accttttgca
     6421 agcaattaaa ttttgcaatt tgtgatttag gcaaaagtta ctatttctgg ctcgtgaatt
     6481 ctgctctgag ccgtcacgct gagtgagtgc taggctccac tgtgactgac ctgggcgcgc
     6541 cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt gctttgagag gctctaaggg
     6601 cttctcagtg cgttacatcc ctggcttgtt gtccacaacc gttaaacctt aaaggcttta
     6661 aaagccttat atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt
     6721 gctgatttat attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc
     6781 ttagtacgtt agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat
     6841 gagagcttag tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta
     6901 ctatcaacag gttgaactgc tgatcttcag atcggcgcgc cggccggcct acggccagcc
     6961 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
     7021 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
     7081 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
     7141 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaaggacaa
     7201 gggtcgtcca aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt taccaatgtt
     7261 taatcaggct cgcgccaatt tccgcaatct gacggttacg ttcatccatc gtcgcctggc
     7321 tgccggtggt ataaatcacc acaatacggc tcggtttacc atccgggccc agcgccgcaa
     7381 taatgccacg gctgccacgt tcgcccgcac cgcttttatc cgcaataaac cagccagccg
     7441 gcagcgcgct acgcagcagc ggacccgcca ctttatccgc ttccatccaa tcaatcagtt
     7501 gctggcggct tgccagggtc agcagctcac ccgtcagcag tttacgcagg gtggtcgcca
     7561 ttgctgccgg catggtggta tcacgttcat cgttcggaat cgcttcgttc agttccggtt
     7621 cccaacgatc cagacgggtc acatgatcgc ccatgttatg cagaaacgcg gtcagttctt
     7681 tcggaccgcc aatggtcgtc agcagcaggt tcgccgcggt gttatcgctc atggtaatcg
     7741 ccgcgctgca cagttcacgc acggtcatgc catcggtcag atgtttttcg gtcaccgggc
     7801 tatattccac cagatcgttc tggctataat gaatacgacg gcccagttgt tcctggcccg
     7861 catccacacg gctcagaacc gcaccgcaca gcagcacttt aaaggtgctc atcatcggaa
     7921 aacgttcttc cggacgaaaa gattccagaa ttttgccgct gttcagatcc agttcaatat
     7981 agcccacgcg cgcacccagt tgatcttccg catctttcac tttcaccagg gtttccggat
     8041 gcgcaaacac cggcaggcaa aacgccgcaa aaaacggaat cagcgccaca cgaaaatgct
     8101 gaatgctcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggaa ttaaaaaaaa
     8221 gcccgctcat taggcgggct actaatttaa atcgattata cgcgcccggg gagccctggg
     8281 gcacgccctg gcacccctcg gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc
     8341 ttttttcgtt ttggtccggc ctcacaggcc ttaattaacc actgtggtgg ctctctagcg
     8401 cttggcggcg aagacggcgt cgggaatctc ggcctcgctg acgttgggga aatcgcgcag
     8461 cgacgcgcgc gacgcctcgt ccccggtcac catcctcagc ggcgtaccgc tggcggcgtc
     8521 caggtacaac gtggacggcc ccttgcccgg acgctggtag cgccagccgt cggcttccct
     8581 gcccagcacc acgcggcggc cgatatggcg ggcgcccagc cggcgcagca cgtcgcgcgg
     8641 caggaacagc tcggcgaacg ggccggtctc gtcgtccagc ggccgctcgc acagctgctc
     8701 gcccagggcg gtccgctcgc ggccataggc caccttctgc ttccaggtgc ggccgctggc
     8761 ggcgttgccg acctcggaca tccacagccg gtagccggtg tcgcgctccg accagggatt
     8821 gaacaggccg tctatccgac agatgtcgcg ctcgtagtcg aaccagcagt agccggacgt
     8881 cagctggtca tccggcagca tcggactcca gtacgacaca taggcgctgc tccagcgcgc
     8941 cggcagcagc ggcggttccc ggttttccat gcggcctcct cagcgttgca gcgcgtagcg
     9001 caggttctgc gccagcgctt ccggcatcgg cggcagcgac ttgcggcgcg cgtcgaagct
     9061 ctgcacgaac tcggcgctgg acaggtgcat gcgctcctcc accgtctcga accagacccg
     9121 gctgttgtcg gcatggccgc ggaacagctg gaccagcggc agcgcgcgct cctcgaagcg
     9181 cttcagcgcg gccggcacgc cgtcctcggt gcacagcgcc ttcaccagca gctgcgccac
     9241 caccaccgcc atcgtggtgc cgtggccgat ggagaagtgg ccggactgca gcgcgtcgcc
     9301 cagcagcacc agcttgccgt cgtggcagcg gtcgtggctc agggtcatga agttgcgcca
     9361 gccgaggccg ggctggctca ccaggccgtg gccgcccagc tcggcctgga acaccttggc
     9421 gacgtattcg gccgacgcct cttccgacat ctcgcccagg cgggcgcggg cataggtctc
     9481 ctcgctgcac tcgacgatga aggtgctcat cgtgtccgag tacttgtagg cgtgggcgat
     9541 gaaaatgtcc ttgccgtggg tgcggaacac caggttcatc tggtcgaaca gctggctggt
     9601 gccgtaccag atgtacttgt tgcggccgta gtccacctgc ggcaccagcg cctcggtgaa
     9661 gtgggcggtc ttgtgattga cgccgttggc cagcaccacc aggtcgtagt cggccagcgg
     9721 cagctcgcca tgctccagca gcggcgattc gaagcggatg gcgatgccct gcgagcggca
     9781 cttgtcgcgc aaggcgtgca ccaggccgcg gcgctccacg ccgcacagca gcacgccggt
     9841 gctcatcagg ctgggctcgt tgtggtggac cagcttgaag tcttccagga actgcggatt
     9901 cagcctctcc ggcgcgtcca ggtaggacag cggattggcc ggatgctggc cgggccggcc
     9961 gggcagcacc acgccccagc ccagcacttc ctgctcgtcg ttcttttcga cgatgtctat
    10021 cgcccacagc ggacgcgcct gtttcagttg gctggcgaac accaggccgg ccggccccgc
    10081 gccgatgacc agaatcttca tcagttgacc ctccctatct tgtaccaaac gttctgtttt
    10141 ctctggatct gctgcagtct gtcgtacggc tcggacgtga agtacagctt ctccgccatg
    10201 tccggcgggt aaaggccggg aaacttgcta tgcatgtagc gggtgtagcg ggcccgcatg
    10261 aagaagatgg gattggagca gctgagcacg tcgtagttgg cgcgcgccat gtcctgcatc
    10321 gcgtcggcct gcaccttgcg cagctcggtg aactcgggaa aggccttgtc ctggtcgccc
    10381 tggtggcggt ccagcagctc gacgaaggtg cgcgcgtcct ccagcgccat gttcatgccc
    10441 tggccgagga aaggcgcggt ggcgtgggcg gcgtcgccca gcagcagcac attgcccttg
    10501 tagtggaagg tgctggaacg gacgttgatc aggtcgttgc tgggcttggc caggaactgg
    10561 cgcagcatct cgtcgcgcgc gtcccgcggc aggccgccga agtaacggtc gaaaaaggcg
    10621 cgcatcgtcg gctcgtcggt ggtggtcagg ctggggctgc cgctgtacgg caggcagacc
    10681 gcgatgctga cgctgccgtc cgggatggtg gcggcgcggc cggcgaacag gccgccggag
    10741 tccatgccga agaaatacag cgtgtccttg cggtagccca gcgcctgcgc gtccggcagc
    10801 accagcgtct tgtagccgtg gcggaagaaa gtctgctgga attcgaagcg gcgcaacccg
    10861 ctctgcatcg cctgccgcac cgccgagtgc gcgccgtcgg cgccgatgat catatcgccc
    10921 tgcaagcgct gcggctggcc gtccttgccc tggatcagca ccgacttgcc gtccagatcc
    10981 acgtccaggc acttgtgctc gaagtagtag cggacgccgg ccaggttggc gtacttgttc
    11041 agcagcttct gaaacgccgc gcggttcagc gacagcgggc ggaaatcctc cagcggcttg
    11101 agctcccgca tccggtactg gccgccgacg gaaaacgcca tcgccacgat gggttcgccg
    11161 caggcgtcca gctccgcgcg cggaatgccg gccgccagca ccgacttgat gccgcgcacg
    11221 gtcatgctga cgcctatcgc ccgcgagctg accacatcca cgtaggaaga caggtcccgc
    11281 agcgggtcgc cgcgcttttc caccacgtgg acctcgtagc cgcgcttcgc caggtagatg
    11341 gcggtcagcc cgccggcgag cccgcctccg actatgattg ctcttttcat ttcaggcctc
    11401 tctagaaagc tttccacaag ccaaatccag catctgccgg cggtagaaat ccagcagttc
    11461 catttgcgcg tcgggcagcc agcccggctc cagcatcgac gcctgctcca gcaggcgctc
    11521 gcagcgccgc gccagcatcc ggcagcccag cgcgtagtcg tcgtagctgc gggtatccac
    11581 cggccccggc agcggcggcc cggcggtgcg tccggcgatg cccgacggca ggttcatcag
    11641 cgcgcaggac agcggcctga gcaggccggt catcaggtcg atcgccgcgt tcatcagccg
    11701 cgagcgcctg aggctgccca gcggcttgac cgcgaagtgc tgcgccatca tcgcgaacat
    11761 cagctcgtac acgccctggt acagcgccat cagcgcgcgc gcccgtccgt ccgccacgcg
    11821 ctggcagccc ggcgactcgt ccagcaccgg gttgcgcagc gccggcaacg ccggctcgaa
    11881 cggcgcggac tgcgccatga tcctggccga catctcccgc agccgctgga aatgcgaatg
    11941 ctcgtaatgc ggcgagtcca gcgccccgcc ctcgccctgg tcggtgacga aggcgatgcc
    12001 gaacagcgcg ctgtcgcgat cgaacacctc cagctggtag ccgggatggg cgcggttggt
    12061 cagctcgttg aggaacaagt ggtgctcgcc gccgcgcttg ccggcctcgt cgccgaacag
    12121 gtcgggcaga tcgagaaagg cctggcggat ggcggcgtag cagtcggcga tggatttgcc
    12181 cggcgcaggg atgaagtgcg gccattccag ccggacgaag cgcgccagcg tcgactcgga
    12241 gaacggctcc agcgcgaatt cggtgtccag gccgaacgcc tgccgcgccg cctcgcccat
    12301 cagcggcacg ccggcgtaga acggctcgcc cagcgccatc agcaggttgt tgaccaccag
    12361 gtaatggatc atctcctcgt gggcgatctc cagcagcgcg gcgcggatgc cgccgtcgcg
    12421 gcgccggtcg ccgctgccgc aggccagctg cagctgctcc gccgtccacg cgccgtcgcg
    12481 cacccgctgc tggccctggg cgtaattggg aatggaatag gcggcgtaca ggtactgcag
    12541 catcaccgac aactccagat ccaccgcctt gcgcagctcg gccgccagct gggccttgct
    12601 ctcgatgcgc gccggcccgg ccggcggcgg cgcctgcagc ctggcctggc cctcgacatg
    12661 ggccaggtat ttgaggaaca gcctggcctt gggcgccgac agctcgcggg tgctgggcat
    12721 gtagtagctc ttgttccggt tctgcggatc gcacatctgc cacatcagcc tggcgtaggt
    12781 ctcgcacttg cagcggtcgg ccaggctgaa caccttgtcg gacatgaacg ggtagaccag
    12841 ctcgtaatag gccatcacgt gccggtacag gaaggcgtaa tcgacctcct ccaccgtcgc
    12901 gtcgtccaac gcccagtcgt ccggcagcac ccgcagcggg atcgcctccg cgccgtcgtc
    12961 gagcacgatg cgcgccgggc cgggccgcag gccggtcagc cgccattcgg cggcattgcc
    13021 gtcctggcgc gactccacgc cgaccagacc catcccctcg atccggtggg gaatgtccgg
    13081 gcgcgcgcgg gcctcgccgc ggaaatagct gcgcagcgcg atgctttccg gaaagaaacg
    13141 gccgtggcgg cggtccggcg cttccaaata aagattggag gcgtcggact gcgtgaccca
    13201 gtcctgctcg cgccattcgg ccagctcgct ggacagcgtc agcgagcccc tgggctcgcg
    13261 cagcagcggc aggtcgacga tgccgtggtt cgtccagtaa tcctggtaaa gcgcctgcgg
    13321 cacccgcgcc agcaacgcgc cgtcctcgtc gcgcagcagc aggtcgccca acggcagctt
    13381 ggccccgaga tcgggcgtca gcctgtccgg cgcggacggc tgcgccgccc gggtggagaa
    13441 cggaatggcg caggccagat tcagcgccac gcggccgccg cttacgcgca gcgtcagatc
    13501 gcccagcccg ggctggcgcg gacgcagcag ccggccggcc gggtagctgg ccagttcgcc
    13561 gcgccgccac aggccgacca cgccgaccat gtcgtggaac accggcgagt tgggttgcgg
    13621 cggcgtcgac atattgaaca gcgcgtactg caccgtcagg ccgagcacgt cgtcgtcctc
    13681 cagcgccagc tgcagcctgc gccaggcttc ggaatcgaat ggccccgggt ggaacaggaa
    13741 gtgcggatgg tctttgggca ccgagaactg gaacagccgc gccaggccga actcctcgtc
    13801 caggaaatgg ccgccgcgct cggcgatgtg gccgccgcgc gtccagcgcg cgccgtggct
    13861 gtcgtcgatg tcggcggtga acagccaggg cgtgcccggt ccggcgccgg ccgggctgat
    13921 cgtgaactgc ccggcgtaga tctgcgccgc gtcgcggcgg gtggggtcgc tgtccaccca
    13981 gcgcgcgcgg ttgaaggtgg tgcgcaggta atcgttgtaa tgcccccaca gcgccagcct
    14041 ggcgccgacc aggccgtcgc cgcggtccgc ttcgccgcca tcccactgca cgtggctgac
    14101 ggtggcgctc tcccaggaga aatggttgtt gccggccgcg ttgtagccct cggccaggct
    14161 gaacggccct tccgggtcag cccggccgtc caggccgaaa cgcggcccca gcgaccgcag
    14221 gtggcggtgg aactcggtcg gatggcgcgc gaggtcgaac ggttcgcctg ccatggccac
    14281 cgtattgctg gccatgtcga tgtggccgtg cggatcgcgg ttggcggtgg gcgcgttgac
    14341 ccgcgcccag ccgcggaaat ggatgcgtgg aaaatccaga atgctcatgt gtttcccgaa
    14401 cggtcatggc tgacaagcgc cgcccggaac cgggcggcga cgcgatgcgg cggccggacc
    14461 gttcacgcgg cgatgcgctg cagcagcaga cggctggctt cgcgggcgct gagcaggccg
    14521 ccctccatcc agccgcagtg ctcggtgtag gcgtccgaac aggcgatgat gccgctgtcg
    14581 cggtggctga gcgcggacgg atggtcgata tcgctgtcgc ggcagaactc cacgccatgt
    14641 gcccaatact tgtgcacatg ggcgaggggc tggggaatgc gctcgcgaac gatgcccagc
    14701 gcgctggcca gatgggtgcg gatctgctcc aggtagccgt cctctccttc ggccacgcag
    14761 ccgcgccagt aattggccat ctcgctgtcg gtgtagaaga acaggtactt gtcgcccttg
    14821 aagtagatct tgcgcagcgg gttgtcgacg atcagc
//
