LOCUS       sequence-406798-       15981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Violacein pathway. SGE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15981)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 15981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-Violacein-SGE
FEATURES             Location/Qualifiers
     source          1..15981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      112..151
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            298..406
                     /note="incP origin of transfer"
     rep_origin      425..813
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             2611..4428
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     promoter        complement(4429..4447)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4430..4447)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4450..4483
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4739..5082
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5113..5922
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5180..5199)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5928..6125
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             6262..6279
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6332..6350)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6346..6393
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     6412..6431
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6412..6430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9429..9438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        15137..15228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15229..15297,15298..15981,1..108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aaaccgagcc gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt
       61 aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac attggtaaac cgatacaatt
      121 aaaggctcct tttggagcct ttttttttgg acgacccttg tccttttccg ctgcataacc
      181 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
      241 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
      301 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
      361 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccggcg
      421 cgccgatctg aagatcagca gttcaacctg ttgatagtac gtactaagct ctcatgtttc
      481 acgtactaag ctctcatgtt taacgtacta agctctcatg tttaacgaac taaaccctca
      541 tggctaacgt actaagctct catggctaac gtactaagct ctcatgtttc acgtactaag
      601 ctctcatgtt tgaacaataa aattaatata aatcagcaac ttaaatagcc tctaaggttt
      661 taagttttat aagaaaaaaa agaatatata aggcttttaa agcctttaag gtttaacggt
      721 tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat
      781 tttgagtgac acaggaacac ttaacggctg acatggggcg cgcccaggtc agtcacagtg
      841 gagcctagca ctcactcagc gtgacggctc agagcagaat tcacgagcca gaaatagtaa
      901 cttttgccta aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac atgcttataa
      961 tcaacttttt taaaaattta aaatactttt ttatttttta tttttaaaca taaatgaaat
     1021 aatttattta ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt
     1081 ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa tgccggctta cattttatgt
     1141 tagctggtgg actgacgcca gaaaatgttg gtgatgcgct tagattaaat ggcgttattg
     1201 gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa agactctaac aaaatagcaa
     1261 atttcgtcaa aaatgctaag aaataggtta ttactgagta gtatttattt aagtattgtt
     1321 tgtgcacttg cctgcaagcc ttttgaaaag caagcataaa agatctaaac ataaaatctg
     1381 taaaataaca agatgtaaag ataatgctaa atcatttggc tttttgattg attgtacagg
     1441 aaaatataca tcgcaggggg ttgactttta ccatttcacc gcaatggaat taaacttgtt
     1501 gaagagaatg ttcacaggcg catacgctac aatgacccga ttcttgctag ccgaattcca
     1561 gtcaggctgc tagcaccaga gctacgtgac cgcaggacta gctccagctg agcgacagcg
     1621 gcaagaaagc catccacgcc gaaaaccccg cttcggcggg gttttgccgc atcttgccgt
     1681 aactgacaaa ttaaccgaag gtttcttccg gccactggga tgcaatgacc ttaccaacaa
     1741 cgctacagga ttcgttgcac gggatcattg ggtattgcgg attcagcggc tgcaggaaaa
     1801 cttggccaga gtcgcggatc agcttcttaa aggtgaattc atcaccaccc aggcgcgcaa
     1861 tgcaaaagtc tcccggctca accgcctgct caggatcaac caggatcagc ataccatccg
     1921 gaaatgacgg cttgctgccc gtcggggccg tcatgctgtt gccttctact tccagccaaa
     1981 acgcgctatc gctcgctttt ttggtagtcg atacccaacg ctcagcgtca cctttggtga
     2041 aggtacgcag ttccgggcta aacatacctg cttgaacgtg gctgaagacc gggtattcgt
     2101 attcagagcg cagagacggt tgcatgctaa ccgcttcata catttcatag atttcacgag
     2161 ctatagaagg agagaactcc tcgacggaaa ctttcagaat cttggtcagc agtgcggcgt
     2221 tgtacgcgtt cagcgcatta ataccgttaa acagagcgcc aacgccgctc tgacccatgc
     2281 ccatcttgtc ggctacgctt tcctgagaca gacccagctc attctttttc ttctcataga
     2341 ttgctttcag acgacgtgcg tcctctagct gctcttgtgt aagcggcttt ttcttcgttg
     2401 acattttgat cctcctttat atggaggtac taaatcggaa cgttaaatct atcaccgcaa
     2461 gggataaata tctaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg
     2521 catcaaattt gcgcgccaca ttattattca tacctttgtg gaccgtatta caaagagaag
     2581 tgttaaatca aacaaaaagg aggattaatc atggacacgt acgcgggtgc ttacgaccgt
     2641 cagtcgcgcg agcgcgaaaa ttcgagcgca gcaagcccag cgacacagcg tagcgccaac
     2701 gaagacaagg cggccgacct tcagcgcgaa gtcgagcgcg acgggggccg gttcaggttc
     2761 gtcgggcatt tcagcgaagc gccgggcacg tcggcgttcg ggacggcgga gcgcccggag
     2821 ttcgaacgca tcctgaacga atgccgcgcc gggcggctca acatgatcat tgtctatgac
     2881 gtgtcgcgct tctcgcgcct gaaggtcatg gacgcgattc cgattgtctc ggaattgctc
     2941 gccctgggcg tgacgattgt ttccactcag gaaggcgtct tccggcaggg aaacgtcatg
     3001 gacctgattc acctgattat gcggctcgac gcgtcgcaca aagaatcttc gctgaagtcg
     3061 gcgaagattc tcgacacgaa gaaccttcag cgcgaattgg gcgggtacgt cggcgggaag
     3121 gcgccttacg gcttcgagct tgtttcggag acgaaggaga tcacgcgcaa cggccgaatg
     3181 gtcaatgtcg tcatcaacaa gcttgcgcac tcgaccactc cccttaccgg acccttcgag
     3241 ttcgagcccg acgtaatccg gtggtggtgg cgtgagatca agacgcacaa acaccttccc
     3301 ttcaagccgg gcagtcaagc cgccattcac ccgggcagca tcacggggct ttgtaagcgc
     3361 atggacgctg acgccgtgcc gacccggggc gagacgattg ggaagaagac cgcttcaagc
     3421 gcctgggacc cggcaaccgt tatgcgaatc cttcgggacc cgcgtattgc gggcttcgcc
     3481 gctgaggtga tctacaagaa gaagccggac ggcacgccga ccacgaagat tgagggttac
     3541 cgcattcagc gcgacccgat cacgctccgg ccggtcgagc ttgattgcgg accgatcatc
     3601 gagcccgctg agtggtatga gcttcaggcg tggttggacg gcagggggcg cggcaagggg
     3661 ctttcccggg ggcaagccat tctgtccgcc atggacaagc tgtactgcga gtgtggcgcc
     3721 gtcatgactt cgaagcgcgg ggaagaatcg atcaaggact cttaccgctg ccgtcgccgg
     3781 aaggtggtcg acccgtccgc acctgggcag cacgaaggca cgtgcaacgt cagcatggcg
     3841 gcactcgaca agttcgttgc ggaacgcatc ttcaacaaga tcaggcacgc cgaaggcgac
     3901 gaagagacgt tggcgcttct gtgggaagcc gcccgacgct tcggcaagct cactgaggcg
     3961 cctgagaaga gcggcgaacg ggcgaacctt gttgcggagc gcgccgacgc cctgaacgcc
     4021 cttgaagagc tgtacgaaga ccgcgcggca ggcgcgtacg acggacccgt tggcaggaag
     4081 cacttccgga agcaacaggc agcgctgacg ctccggcagc aaggggcgga agagcggctt
     4141 gccgaacttg aagccgccga agccccgaag cttccccttg accaatggtt ccccgaagac
     4201 gccgacgctg acccgaccgg ccctaagtcg tggtgggggc gcgcgtcagt agacgacaag
     4261 cgcgtgttcg tcgggctctt cgtagacaag atcgttgtca cgaagtcgac tacgggcagg
     4321 gggcagggaa cgcccatcga gaagcgcgct tcgatcacgt gggcgaagcc gccgaccgac
     4381 gacgacgaag acgacgccca ggacggcacg gaagacgtag cggcgtaatc tatagtgtca
     4441 cctaaatggg tgccagggcg tgcccgtggg ctccccgggc gcgtataggg ataacagggt
     4501 aatacgtcaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
     4561 acgccaactt tgaaaacaac tttgaaaagg ctgttttctg tatttaaggt tttagaatgc
     4621 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
     4681 ctgtagaaaa agaaattgca tacctttgtt cctcggttat atgtttgctc atctgcaaga
     4741 catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg
     4801 tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg catccataca
     4861 ttttgatggc cgcacggcgc gaagcaaaaa ttacggcacc tcgctgcaga cctgcgagca
     4921 gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa
     4981 tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt
     5041 gctaggatac agttctcaca tcacatccga acataaacaa ccaaaaattt gtaattaaga
     5101 aggagtgatt acatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac
     5161 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     5221 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     5281 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     5341 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     5401 actgcgatcc ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     5461 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     5521 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     5581 ggtttggttg atgtgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     5641 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     5701 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     5761 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     5821 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     5881 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa
     5941 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     6001 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgtccagatg
     6061 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     6121 tactgctcta gcataacccc gcggggcctc tttcggggat ctcgcggggt tttttgctga
     6181 aagaagcttc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttatgttgtt
     6241 gtcgctgcgg ccgcactcga gcaccaccac caccaccact gggatccggc tgctaacaaa
     6301 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     6361 ggggcctcta aacgggtctt gaggggtttt ttgctgaagg ccatcatggc ctaatacgac
     6421 tcactatagg gagaccaatg tgagtttttt tttttatttt tccgcgcccc tccttgaagg
     6481 gggcggtgta gcatgtgaac tgaaatttac agaggggcaa tttttgtttt ttcattaacg
     6541 tttaacacta taaaagcgcg gtggctccat taaattgctc cttccttttt tataacacca
     6601 agcaactaat ttttaactaa gtaatttcga gtaatcgacg ttataatagg aggatcaaaa
     6661 tgaaacactc atcagacatt tgtatagtag gcgcaggtat ttccggtctc acctgtgcga
     6721 gccacctgct ggattcccca gcttgtcgtg gcctgagcct gcgcattttc gacatgcaac
     6781 aggaggccgg tggccgcatt cgcagcaaga tgctggacgg taaggcaagc atcgaactgg
     6841 gcgcaggccg ctacagcccg cagctgcacc cgcacttcca aagcgctatg caacattatt
     6901 cgcagaaaag cgaagtctat ccgttcaccc aactgaagtt caagtctcac gtacaacaga
     6961 aactgaagcg tgcgatgaac gaactgtccc cgcgtctgaa agagcacggt aaagaaagct
     7021 ttctgcagtt cgtttcccgc taccaaggtc acgacagcgc tgtaggtatg atccgcagca
     7081 tgggttatga cgcactgttt ctgccggaca tctcggctga aatggcgtat gatattgttg
     7141 gcaaacaccc ggaaattcaa tccgttaccg acaacgatgc aaatcagtgg ttcgcagccg
     7201 aaacgggttt cgcgggtctg atccaaggca tcaaggccaa ggttaaggcg gcgggcgcgc
     7261 gtttcagcct gggctaccgc ctgctgagcg tacgtaccga cggcgacggt tacctgctgc
     7321 agctcgctgg cgacgacggt tggaagctgg aacatcgtac ccgccacctg atcctggcga
     7381 tcccgcctag cgcgatggcg ggtctgaacg ttgactttcc ggaggcgtgg agcggcgcac
     7441 gttacggctc actgccgctg ttcaaaggct tcctgaccta cggtgagccg tggtggctgg
     7501 actataaact ggacgaccag gtactgatcg tcgacaatcc gctgcgtaag atctacttta
     7561 aaggcgacaa gtacctgttc ttttacactg atagcgaaat ggctaactat tggcgcggtt
     7621 gtgtcgccga gggtgaagac ggttatctgg aacagattcg tacccacctg gcgagcgcgc
     7681 tgggcatcgt tcgtgaacgt atcccgcagc cgctggcaca cgtacacaag tattgggcac
     7741 acggtgttga attttgccgt gattccgata tcgatcaccc gtctgcgctg tctcaccgtg
     7801 atagcggtat catcgcctgc tcggatgcct ataccgaaca ctgcggttgg atggaaggtg
     7861 gtctgctgtc cgcgcgtgaa gcgagccgtc tgctgctgca gcgcatcgcg gcctaagttg
     7921 aaaccttata taactgtcta gaaataaaga gtatcatctt tcaaatcaga atagtcattt
     7981 tttttttttt ttaccgcgcc cctccttgaa actgaaattt tagcatgtga gggggcggtg
     8041 gctcaacggc aactcctttt taatcatcat aaaaatttta tataaaagcg taggagtact
     8101 cgatggtaca gatgagcatt tttatacggt caacgaacta ttttttaagt aactaataac
     8161 cattatcacc atttaattta ggagctttaa aatgtccata ctggattttc cgaggataca
     8221 ctttcgtggc tgggcgcgtg taaacgcccc gaccgccaac cgtgacccgc acggtcacat
     8281 tgatatggcg tctaacaccg ttgctatggc gggcgaaccg ttcgacctgg ctcgccaccc
     8341 gacggaattc caccgtcatc tgcgctcact gggtccgcgc tttggtctgg acggtcgtgc
     8401 agatccggaa ggtccattta gcctggcgga aggctataac gcggcgggca acaaccactt
     8461 tagctgggaa agcgccactg taagccacgt acaatgggac ggtggcgaag ctgaccgtgg
     8521 tgacggtctg gtcggtgccc gtctggcact gtggggtcac tataacgatt atctgcgcac
     8581 tacgtttaac cgcgcacgtt gggttgactc cgatccgacg cgtcgtgacg cagctcagat
     8641 ttatgcgggc caatttacca tcagcccggc tggcgcaggt ccgggtacgc cttggctgtt
     8701 taccgcggat attgacgata gccatggtgc gcgctggacc cgtggtggtc acatcgcaga
     8761 acgtggcggt cacttccttg acgaagagtt tggtctggcg cgtctgtttc agttcagcgt
     8821 tccaaaagac cacccgcact tcctgttcca cccgggcccg tttgatagcg aagcctggcg
     8881 tcgtctgcag ctggctctgg aggatgatga cgttctgggc ctgaccgttc aatacgcgct
     8941 gtttaacatg tcgacgccgc cacagccgaa cagcccggta tttcatgaca tggttggcgt
     9001 tgtcggtctt tggcgccgtg gtgagctggc gagctacccg gcaggccgtc tcctgcgccc
     9061 tcgtcagccg ggtctgggtg atctgaccct gcgtgttagc ggcggccgtg tagcactgaa
     9121 cctggcttgt gctattccat tcagcactcg tgcggcgcag ccgagcgcgc cggaccgtct
     9181 gaccccggat ctgggcgcca agctgccgct gggcgacctg ctgcttcgtg atgaagatgg
     9241 cgctctgctg gcacgtgtcc cgcaggcact gtaccaagat tattggacga accatggtat
     9301 cgtagacctg ccgctgctgc gtgaaccgcg tggctccctt accctgagct ctgagctggc
     9361 cgaatggcgt gaacaggact gggttacgca atccgacgca tccaatctgt acctggaagc
     9421 gccggatcgc cgccatggcc gcttctttcc agaaagcatt gcactgcgtt cgtacttccg
     9481 cggcgaagcc cgcgcgcgtc cggatatacc gcaccgtatt gaaggtatgg gtctggtcgg
     9541 tgtagaaagc cgtcaagatg gcgacgccgc agaatggcgt ctgaccggtc tgcgtccggg
     9601 cccggcacgt attgttctgg atgacggcgc agaagctatc ccgctgcgtg ttctgccgga
     9661 cgattgggca ctggatgatg cgacggtaga agaggtagat tacgcgtttc tgtatcgcca
     9721 cgttatggcc tactacgaac ttgtctaccc gtttatgtct gacaaggttt tttccctggc
     9781 agaccgttgc aagtgcgaaa cttacgcacg cctgatgtgg caaatgtgcg acccgcagaa
     9841 ccgcaacaaa agctactata tgccgtccac ccgagagctg agcgcaccaa aggcgcgcct
     9901 gttcctgaaa tacctggccc atgttgaagg tcaagcgcgt ctccaggcac cgccgccggc
     9961 aggtcctgcg cgtatcgagt ccaaagcgca actggctgcc gaactgcgca aggcagttga
    10021 cctggagctg agcgttatgc tgcagtacct gtacgctgcg tatagcatcc ctaactacgc
    10081 gcaaggtcag caacgtgtcc gtgacggtgc ctggacggcg gaacagctgc aactggcgtg
    10141 cggttcgggc gatcgccgcc gtgatggcgg tatccgcgcc gcgctgctgg agatcgcgca
    10201 cgaagaaatg atccactatc tggtagttaa caatctgctg atggcactgg gtgaaccgtt
    10261 ttacgcaggt gttccgctga tgggcgaagc ggctcgtcaa gcgttcggtc tggacaccga
    10321 attcgccctg gagccgttca gcgaatcgac cctggcacgc ttcgttcgcc tggaatggcc
    10381 gcacttcatt cctgcgccag gcaagtccat cgccgactgc tacgctgcga tccgccaagc
    10441 attcctggat ctgccggacc tgttcggcgg tgaggcgggc aaacgtggtg gcgaacacca
    10501 cctgttcctg aacgaactga ccaaccgcgc gcacccgggt taccagctgg aagtatttga
    10561 tcgtgacagc gcgctgttcg gtatcgcgtt cgttaccgat caaggcgaag gtggcgcgct
    10621 ggacagcccg cactatgaac acagccattt tcagcgtctg cgcgaaatga gcgcccgtat
    10681 catggcgcag agcgcgccgt ttgagccggc actgccagcg ctgcgtaacc cggttctgga
    10741 tgaaagcccg ggttgtcaac gcgttgcgga cggccgcgca cgcgccctga tggcgctgta
    10801 ccaaggtgta tacgaactga tgttcgcgat gatggcccag catttcgcgg ttaaaccgct
    10861 gggcagcctg cgtcgtagcc gtctgatgaa tgcggcgatt gacctgatga cgggcctgct
    10921 gcgtccgctg tcctgcgccc tgatgaacct gcctagcggt attgcaggtc gtaccgccgg
    10981 tccgccgctg ccaggtccgg ttgacacgcg tagctacgat gattatgcac tgggttgccg
    11041 catgctggcc cgtcgttgcg aacgcctgct ggaacaagct tccatgctgg aaccgggttg
    11101 gctgccggat gcgcagatgg aactgctgga tttctaccgc cgccagatgc tggacctggc
    11161 gtgcggtaaa ctgtcccgcg aggcctaata tataaactca tttacttatg taggaataaa
    11221 gagtatgtct cccgcgccac agaggggcgg gggcggtgac tgaaattttt tttgacaagt
    11281 caagtcagga aaaaaaaata taaaagggcg ctgcgtaagg agtgctgcca ggtggttttt
    11341 taagaaatca aaagaataat ttttaagtaa ctaatcaatc catacaactc tattataagg
    11401 agcgggaaaa tgaaaagggc aataatcgta ggaggcggac tcgcaggcgg tctgaccgca
    11461 atttacctgg cgaaacgtgg ttacgaagtt cacgtcgttg aaaagcgtgg tgaccctctg
    11521 cgcgatctga gctcttacgt agatgtcgtc tcctcccgtg cgatcggcgt cagcatgacc
    11581 gtacgtggca tcaagagcgt tctggcggca ggtatcccgc gtgcagagct ggatgcgtgt
    11641 ggtgagccga tcgttgcgat ggccttcagc gttggtggtc aataccgcat gcgcgaactg
    11701 aagcctctgg aagacttccg tccgctgtcc ctgaaccgtg cggcctttca aaagctgctg
    11761 aacaagtacg cgaacctggc gggtgttcgc tactacttcg aacacaaatg cctggacgtt
    11821 gacctggatg gcaaaagcgt cctgattcag ggcaaagatg gtcaaccgca gcgtctccag
    11881 ggcgatatga tcatcggcgc ggacggcgcg cactctgcag tacgtcaagc gatgcagtcg
    11941 ggcctgcgtc gctttgaatt tcaacaaacc ttcttccgtc acggctataa aacgctggta
    12001 ctgccggacg ctcaagcgct gggttatcgt aaagacaccc tgtacttctt tggtatggac
    12061 tctggtggcc tgtttgcggg ccgcgcggcg accatcccgg acggttcagt ttctattgcg
    12121 gtatgcctgc cgtacagcgg tagcccgagc ctgacgacga ccgacgagcc gacgatgcgc
    12181 gcttttttcg accgttactt tggcggcctg ccgcgtgacg cccgcgatga aatgctgcgc
    12241 cagtttctgg ccaaaccgtc caacgatctt atcaacgtcc gttcttcaac cttccattac
    12301 aagggtaatg ttctcctgct gggcgatgca gcgcacgcga cggcaccgtt cctgggccag
    12361 ggtatgaata tggctctgga ggatgcgcgt accttcgttg agctgctgga ccgccaccag
    12421 ggtgaccagg acaaggcatt tcctgagttc acggaactgc gtaaagttca agcagacgcg
    12481 atgcaagaca tggcgcgtgc gaactatgac gttctgtcct gcagcaaccc gatctttttc
    12541 atgcgtgcac gttatacccg ctacatgcac agcaagttcc ctggtctgta tccgccggac
    12601 atggcagaaa aactgtactt caccagcgaa ccgtacgacc gtctgcagca aattcagcgt
    12661 aaacaaaacg tatggtacaa gataggaaga gtcaactaat atataactgt ctagaaataa
    12721 agagtatcat ctaaaaattc tacttttttt tttttccgcg ccacagaggg gccctccttg
    12781 aagggggcgg tgactgaaat tttagcatgt gaaaaaattt tttttaatcc tcatttttta
    12841 aatataaaag ggtccctggg tttgcgtact ttatccgtca ttttttttct ttcataacac
    12901 caatttttaa ctaagtaaac atcattcgaa agcgtaaata taggagtact aaaatgaaaa
    12961 tccttgtaat aggagcagga ccggcaggcc tggtcttcgc aagccagctg aagcaggctc
    13021 gtccgctgtg ggcgatcgat atagtcgaaa agaacgatga acaggaggtc ctgggttggg
    13081 gtgtagtact gccgggtcgt ccgggccagc atccggcaaa cccgctgagc tatctggacg
    13141 caccggaacg tctgaacccg caatttctgg aagacttcaa actggttcac cacaatgaac
    13201 cgagcctgat gtctaccggc gtactgctgt gcggtgttga gcgtcgtggt ctggtccacg
    13261 cgctgcgtga taaatgccgc agccaaggca tcgcgatccg tttcgaatcc ccgctgcttg
    13321 aacatgggga actgccgctg gctgattatg acctggttgt cctggcgaac ggtgttaacc
    13381 ataagaccgc gcactttacg gaagcgctgg ttccgcaagt tgactacggc cgtaacaagt
    13441 atatctggta tggtaccagc caactgtttg atcaaatgaa cctggttttc cgtacccacg
    13501 gcaaggacat ttttatcgcg catgcataca agtacagcga cacgatgagc acctttatcg
    13561 tagagtgcag cgaggagacc tacgcccgcg cgcgcctggg cgagatgagc gaagaagcaa
    13621 gcgcggaata cgtagctaaa gtttttcagg ctgagctggg tggtcatggc ctggtatccc
    13681 agccgggtct gggttggcgt aactttatga ccctgtccca tgaccgctgc cacgacggaa
    13741 aacttgttct gctgggtgac gcactgcagt ctggccattt cagcatcggc cacggtacca
    13801 ccatggccgt tgtcgtcgca cagctgctgg tcaaagcact gtgcaccgaa gatggtgttc
    13861 cggcggccct gaagcgtttc gaagagcgcg cgctgccgct agttcagctg tttcgcggtc
    13921 atgcagacaa cagccgtgtc tggtttgaga ccgttgaaga acgtatgcac ctgagcagcg
    13981 cggaattcgt tcagtccttt gacgcacgtc gtaaaagcct gccgcctatg ccagaagccc
    14041 tggcccaaaa cctgcgttat gccctgcagc gctaatatat atatataact gtctagaaat
    14101 aaagagtatc atctttcaaa ccgttttttt ttttgcccgc gccacagagg ggcgctcaac
    14161 ggcgggggcg gtgtagcatg tgattttttc taataaccaa actttttttt tgatataaaa
    14221 gccgataggg tgggcgaagg ggcgcaggtc ctttttaaag aattaaatat aatcttttta
    14281 actaactaac agggttcatt cagcttataa aaaggagggt aaaaatggaa aatagggaac
    14341 cgccccttct tcccgcaagg tggagctccg cgtacgtatc ctactggtcc ccgatgctgc
    14401 cggacgacca actgacgtcc ggttactgct ggttcgatta cgaacgcgac atttgccgca
    14461 ttgacggtct gttcaacccg tggagcgaac gtgatacggg ttatcgtctg tggatgagcg
    14521 aggtaggcaa cgccgcatct ggtcgtacct ggaaacagaa ggttgcgtac ggtcgtgaac
    14581 gtacggccct gggtgaacaa ctgtgcgaac gtccgctgga tgacgaaacc ggccctttcg
    14641 ccgagctgtt cctgccgcgc gatgttctgc gccgcctggg tgcgcgtcat atcggccgtc
    14701 gtgttgtcct gggccgcgaa gcggatggct ggcgttacca gcgtccaggc aagggccctt
    14761 ctacgctgta cctggatgcg gcgtccggca cccctctgcg tatggttacc ggtgacgaag
    14821 ccagccgcgc atctctgcgt gactttccaa acgtaagcga ggcagaaatc ccggacgccg
    14881 ttttcgcagc taagcgctaa ggtaactatc tatataagag aatagtaata aatacaatct
    14941 gttttcaaat aacgatagtc cacagtgggg cctgtgaggc cggaccaaaa cgaaaaaagg
    15001 cccccctttc gggaggcctc ttttctggaa tttggtaccg aggggtgcca gggcgtgccc
    15061 cagggctccc cgggcgcgta taatcgattt aaattagtag cccgcctaat gagcgggctt
    15121 ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    15181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag
    15241 cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat
    15301 ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat
    15361 attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt
    15421 ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg
    15481 ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc
    15541 ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg
    15601 attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa
    15661 gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa
    15721 ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg
    15781 gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa
    15841 ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg
    15901 gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt
    15961 gcggcgctgg gcccggatgg t
//
