LOCUS       sequence-406810-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Integrin alpha-IIb precursor ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence 
            and His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: ITGA2B-bio-His
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1262..1641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1642..1845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1796..1816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1842..1866
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2347..2366
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2400..2409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6045..6062
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6114..6169
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6168..6187)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8405..8423)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8491..8595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8596..8664,8665..9456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8814..8833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg
     1081 ctgcaggcgc agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta
     1141 gccatattag tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     1201 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     1261 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     1321 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1381 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     1441 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     1501 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     1561 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     1621 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     1681 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     1741 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     1801 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     1861 cagatcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg ctcgcggttg
     1921 aggacaaact cttcgcggtc tttccagtac tcttggatcg gaaacccgtc ggcctccgaa
     1981 cggtactccg ccaccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc
     2041 gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag
     2101 cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc
     2161 ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg cttgagatcc agctgttggg
     2221 gtgagtactc cctctcaaaa gcgggcatta cttctgcgct aagattgtca gtttccaaaa
     2281 acgaggagga tttgatattc acctggcccg atctggccat acacttgagt gacaatgaca
     2341 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaagtttaa actgcggccg
     2401 ccaccatggc tagagccctg tgcccactgc aggccctgtg gctgctggaa tgggtgctgc
     2461 tgctgctcgg accttgtgcc gctcctcccg cctgggccct gaacctggac cccgtgcagc
     2521 tgaccttcta cgccggaccc aacggcagcc agttcggctt cagcctggac ttccacaagg
     2581 acagccacgg cagagtggcc attgtcgtgg gagcccccag aacactgggc cccagccagg
     2641 aagagacagg cggcgtcttt ctgtgccctt ggagagccga gggcggccag tgccctagcc
     2701 tgctgttcga cctgagggac gagacacgga acgtgggcag ccagaccctg cagaccttca
     2761 aggccagaca gggcctggga gccagcgtgg tgtcttggag cgacgtgatc gtggcctgtg
     2821 ccccctggca gcactggaac gtgctggaaa agaccgagga agccgagaaa acccccgtgg
     2881 gcagctgctt tctggcccag cccgaatcag gcagaagggc cgagtactct ccctgcagag
     2941 gcaacaccct gagccggatc tacgtggaga acgacttcag ctgggacaag cggtactgcg
     3001 aggccggatt cagctctgtg gtcacccagg ccggcgaact ggtgctggga gcacctggcg
     3061 gctactactt tctcgggctg ctggctcagg ctcccgtggc cgacatcttc agcagctaca
     3121 gacccggcat cctgctgtgg cacgtgtcca gccagagcct gagcttcgac agcagcaacc
     3181 ccgagtactt cgacggctac tggggctact ctgtggccgt gggcgagttc gacggcgacc
     3241 tgaacaccac cgagtacgtc gtgggcgctc ctacctggtc ttggaccctg ggcgccgtgg
     3301 agatcctgga cagctactac cagcggctgc acagactgag agccgagcag atggccagct
     3361 actttggcca tagcgtggct gtgaccgatg tgaacggcga cggccggcat gatctgctcg
     3421 tgggcgcacc tctgtacatg gaaagcagag ccgaccggaa gctggccgaa gtgggccggg
     3481 tgtacctgtt cctgcagccc agaggacctc atgccctggg cgctccaagc ctgctgctga
     3541 ccggcaccca gctgtacggc agattcggca gcgccattgc ccctctgggc gacctggacc
     3601 gggacggcta caacgatatc gccgtggccg ctccttatgg cggccctagc ggcagaggcc
     3661 aggtgctggt gttcctgggc cagagcgagg gcctgagaag cagaccctct caggtgctgg
     3721 acagcccctt tcccactggc tctgccttcg gcttttccct gcggggagcc gtggacatcg
     3781 acgacaacgg ctaccccgac ctcatcgtcg gagcctacgg cgctaatcag gtggccgtgt
     3841 acagagccca gcccgtggtc aaggcctctg tgcagctgct ggtccaggat tctctgaacc
     3901 ccgccgtgaa gtcctgtgtg ctgccccaga ccaagacccc cgtgtcctgc ttcaacatcc
     3961 agatgtgcgt cggcgctacc ggacacaaca tcccccagaa gctgtccctg aacgccgagc
     4021 tgcagctgga ccggcagaag cccaggcagg gcagaagagt gctgctcctg ggctctcagc
     4081 aggccggcac caccctgaat ctggatctgg gcggcaagca cagccccatc tgccacacca
     4141 ccatggcctt tctgcgggac gaggccgact tccgggacaa gctgtccccc atcgtgctgt
     4201 ctctgaacgt gtccctgcct cctacagagg ccggaatggc ccctgctgtg gtgctgcacg
     4261 gcgacaccca cgtgcaggaa cagacccgga tcgtcctgga ctgcggcgag gacgatgtgt
     4321 gcgtgcctca gctgcagctc acagccagcg tgaccggctc tcctctgctc gtcggggccg
     4381 acaatgtgct ggaactgcag atggacgccg ccaatgaggg cgaaggcgcc tacgaagccg
     4441 aactggccgt gcatctgcct cagggcgccc actacatgag agccctgagc aacgtggagg
     4501 gcttcgagcg gctgatctgc aaccagaaga aagaaaacga gacaagagtc gtcctgtgcg
     4561 agctgggcaa ccccatgaag aagaacgccc agatcggcat tgccatgctg gtgtccgtgg
     4621 gcaatctgga agaggccggc gagagcgtgt ccttccagct gcagatccgg tccaagaaca
     4681 gccagaaccc taattctaag atcgtgctgc tggacgtgcc tgtcagagcc gaggcccagg
     4741 tggagctgag aggcaacagc ttccccgcct ctctggtggt ggccgccgaa gagggcgaga
     4801 gagagcagaa cagcctggat agctggggac caaaggtgga gcacacctac gagctgcaca
     4861 acaacggccc tggcaccgtg aatggcctgc acctgagcat ccatctgccc ggccagagcc
     4921 agcctagcga tctgctgtac atcctggaca tccagccaca gggcggcctg cagtgctttc
     4981 cacagccccc cgtgaaccct ctgaaggtgg actggggcct gcctattcct tctcctagcc
     5041 ccattcaccc cgcccaccac aagcgggacc ggcggcagat cttcctgcct gaacccgaac
     5101 agccaagcag actccaggat cccgtgctgg tgtcttgcga cagcgccccc tgcaccgtgg
     5161 tgcagtgcga tctgcaggaa atggctcgcg gacagcgggc tatggtcacc gtgctggctt
     5221 ttctgtggct gcccagcctg taccagaggc ccctggacca gttcgtgctg cagagccacg
     5281 cctggttcaa cgtgtcctcc ctgccctatg ccgtgcctcc cctgtctctg cctagaggcg
     5341 aagcccaggt ctggacccag ctgctgcggg ccctggaaga gagagccatc cctggcgcgc
     5401 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     5461 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     5521 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     5581 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     5641 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     5701 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     5761 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     5821 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     5881 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     5941 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     6001 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     6061 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     6121 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     6181 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     6241 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     6301 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     6361 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     6421 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     6481 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     6541 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     6601 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     6661 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     6721 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     6781 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     6841 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     6901 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     6961 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     7021 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     7081 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     7141 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     7201 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     7261 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     7321 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     7381 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     7441 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     7501 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     7561 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     7621 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     7681 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     7741 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     7801 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     7861 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     7921 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     7981 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     8041 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     8101 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     8161 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     8221 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     8281 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     8341 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     8401 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8461 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8821 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     8881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9121 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9601 cccgtagaaa agatcaaagg atcttc
//
