LOCUS       sequence-406944-        7995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to be used as DNA template for in vitro RNA transcription of
            the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid 
            contains optimised 5'UTR and 3'UTR to improve protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Jalil S, Keskinen T, Juutila J, Sartori Maldonado R, Euro L, 
            Suomalainen A, Lapatto R, Kuuluvainen E, Hietakangas V, Otonkoski T,
            Hyvonen ME, Wartiovaara K
  TITLE     Genetic and functional correction of argininosuccinate lyase 
            deficiency using CRISPR adenine base editors.
  JOURNAL   Am J Hum Genet. 2024 Apr 4;111(4):714-728. doi: 
            10.1016/j.ajhg.2024.03.004.
  PUBMED    38579669
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ajhg.2024.03"; journalName: "Am J Hum Genet"; date: 
            "2024-04-4- 4"; volume: "111"; issue: "4"; pages: "714-728"
COMMENT     Sequence Label: pRN3P_T3_ABE8e_IVT
FEATURES             Location/Qualifiers
     source          1..7995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     3'UTR           complement(65..190)
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     CDS             complement(227..247)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(290..4363,4364..4366,4367..4390))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(290..4390)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4985..5005)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     5'UTR           complement(5080..5122)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(5147..5165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5186..5202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5186..5202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5199..5221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5210..5226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5234..5240,5241..5258,5259..5264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5279..5300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5417..5434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5588..6176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5668..5687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6347..7138,7139..7207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6970..6989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7208..7312)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7339..7794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7426..7445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7636..7657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7921..7943
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7935..7952
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7936..7952
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7959..7978
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7959..7977
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacttggccc tgcagggggg ggggggggkk kttttttttt tttttttttt tttttttttw
       61 kwwtgtgaag aaactttytt tttwttagga gcagatacga atggctacat tttgggggac
      121 aacattttgt aaagtgtaag ttggtattat gtagcttaga gactccattc gggtgttctt
      181 gaggctggtt tagtggtaac cagatccgcg gccgctcacc tacttagact ttcctcttct
      241 tcttgggctc gaattcgctg ccgtcggcgg ttctttttga gccgccagag tcacctccca
      301 gctgagacag gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg
      361 cgtccagcac ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact
      421 tgaaggcggc aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct
      481 gctctctgat gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag
      541 cgtcggccag gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt
      601 agtgcttgtg ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca
      661 gcttctcata gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt
      721 cgtttccctt ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct
      781 cgaacaggga gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct
      841 tggcttccag aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc
      901 ccagcagctc tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca
      961 ccagcacaga ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt
     1021 ccttctttct ggcgatcagc ttatcgctgt tcctcttggg caggatagac tctttgctga
     1081 agccgcctgt ctgcacctcg gtctttttca cgatattcac ttggggcatg ctcagcactt
     1141 tccgcacggt ggcaaaatcc cggcccttat cccacacgat ctccccggtt tcgccgtttg
     1201 tctcgatcag aggccgcttc cggatctcgc cgttggccag ggtaatctcg gtcttgaaaa
     1261 agttcatgat gttgctgtag aagaagtact tggcggtagc cttgccgatt tcctgctcgc
     1321 tcttggcgat catcttccgc acgtcgtaca ccttgtagtc gccgtacacg aactcgcttt
     1381 ccagcttagg gtactttttg atcagggcgg ttcccacgac ggcgttcagg taggcgtcgt
     1441 gggcgtggtg gtagttgttg atctcgcgca ctttgtaaaa ctggaaatcc ttccggaaat
     1501 cggacaccag cttggacttc agggtgatca ctttcacttc ccggatcagc ttgtcattct
     1561 cgtcgtactt agtgttcatc cgggagtcca ggatctgtgc cacgtgcttt gtgatctgcc
     1621 gggtttccac cagctgtctc ttgatgaagc cggccttatc cagttcgctc aggccgcctc
     1681 tctcggcctt ggtcagattg tcgaactttc tctgggtaat cagcttggcg ttcagcagct
     1741 gccgccagta gttcttcatc ttcttcacga cctcttcgga gggcacgttg tcgctcttgc
     1801 cccggttctt gtcgcttctg gtcagcacct tgttgtcgat ggagtcgtcc ttcagaaagc
     1861 tctgaggcac gatatggtcc acatcgtagt cggacagccg gttgatgtcc agttcctggt
     1921 ccacgtacat atcccgccca ttctgcaggt agtacaggta cagcttctcg ttctgcagct
     1981 gggtgttttc cacggggtgt tctttcagga tctggctgcc cagctctttg atgccctctt
     2041 cgatccgctt cattctctcg cggctgttct tctgtccctt ctgggtggtc tggttctctc
     2101 tggccatttc gatcacgatg ttctcgggct tgtgccggcc catcactttc acgagctcgt
     2161 ccaccacctt cactgtctgc aggatgccct tcttaatggc ggggctgccg gccagattgg
     2221 caatgtgctc gtgcaggcta tcgccctggc cggacacctg ggctttctgg atgtcctctt
     2281 taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt tctgttggcg aagccgtcgg
     2341 acttcaggaa atccaggatt gtcttgccgg actgcttgtc ccggatgccg ttgatcagct
     2401 tccggctcag cctgccccag ccggtgtatc tccgccgctt cagctgcttc atcactttgt
     2461 cgtcgaacag gtgggcatag gttttcagcc gttcctcgat catctctctg tcctcaaaca
     2521 gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt ttcctcattg tccaggaagt
     2581 ccttgtcctt gataattttc agcagatcgt ggtatgtgcc cagggaggcg ttgaaccgat
     2641 cttccacgcc ggagatttcc acggagtcga agcactcgat tttcttgaag tagtcctctt
     2701 tcagctgctt cacggtcact ttccggttgg tcttgaacag caggtccacg atggcctttt
     2761 tctgctcgcc gctcaggaag gcgggctttc tcattccctc ggtcacgtat ttcactttgg
     2821 tcagctcgtt atacacggtg aagtactcgt acagcaggct gtgcttgggc agcaccttct
     2881 cgttgggcag gttcttatcg aagttggtca tccgctcgat gaagctctgg gcggaagcgc
     2941 ccttgtccac cacttcctcg aagttccagg gggtgatggt ttcctcgctc tttctggtca
     3001 tccaggcgaa tctgctgttt cccctggcca gagggcccac gtagtagggg atgcggaagg
     3061 tcaggatctt ctcgatcttt tcccggttgt ccttcaggaa tgggtaaaaa tcttcctgcc
     3121 gccgcagaat ggcgtgcagc tctcccaggt ggatctggtg ggggatgctg ccgttgtcga
     3181 aggtccgctg cttccgcagc aggtcctctc tgttcagctt cacgagcagt tcctcggtgc
     3241 cgtccatctt ttccaggatg ggcttgatga acttgtagaa ctcttcctgg ctggctccgc
     3301 cgtcaatgta gccggcgtag ccgttcttgc tctggtcgaa gaaaatctct ttgtacttct
     3361 caggcagctg ctgccgcacg agagctttca gcagggtcag gtcctggtgg tgctcgtcgt
     3421 atctcttgat catagaggcg ctcagggggg ccttggtgat ctcggtgttc actctcagga
     3481 tgtcgctcag caggatggcg tcggacaggt tcttggcggc cagaaacagg tcggcgtact
     3541 ggtcgccgat ctgggccagc aggttgtcca ggtcgtcgtc gtaggtgtcc ttgctcagct
     3601 gcagtttggc atcctcggcc aggtcgaagt tgctcttgaa gttgggggtc aggcccaggc
     3661 tcagggcaat caggtttccg aacaggccat tcttcttctc gccgggcagc tgggcgatca
     3721 gattttccag ccgtctgctc ttgctcagtc tggcagacag gatggccttg gcgtccacgc
     3781 cgctggcgtt gatggggttt tcctcgaaca gctggttgta ggtctgcacc agctggatga
     3841 acagcttgtc cacgtcgctg ttgtcggggt tcaggtcgcc ctcgatcagg aagtggcccc
     3901 ggaacttgat catgtgggcc agggccagat agatcagccg caggtcggcc ttgtcggtgc
     3961 tgtccaccag tttctttctc aggtggtaga tggtggggta cttctcgtgg taggccacct
     4021 cgtccacgat gttgccgaag atggggtgcc gctcgtgctt cttatcctct tccaccagga
     4081 aggactcttc cagtctgtgg aagaagctgt cgtccacctt ggccatctcg ttgctgaaga
     4141 tctcttgcag atagcagatc cggttcttcc gtctggtgta tcttcttctg gcggttctct
     4201 tcagccgggt ggcctcggct gtttcgccgc tgtcgaacag cagggctccg atcaggttct
     4261 tcttgatgct gtgccggtcg gtgttgccca gcaccttgaa tttcttgctg ggcaccttgt
     4321 actcgtcggt gatcacggcc cagcccacag agttggtgcc gatggccagg ccgatgctgt
     4381 acttcttgtc tgaccccccg ctgctgcccc cgctgctttc aggtgttgcg ctctcgcttg
     4441 tgccaggtgt ctcagagcca gaggagcctc cgctagatcc tccggagttg atggagctct
     4501 gggccttctt ctgagcattg aacacctgtc taggcatccg atagaaatcg cacagcaggg
     4561 cggcacattc atctgccagg attccctcgg taatttcgac gcggtgattc atgccggggt
     4621 agttcagcac gttcatcagg gagcctgcgg cgcctctttt tgagttcctc acgccaaaca
     4681 ccacgcggcc gatcctagag tggatcatgg cgccggcgca catcacgcaa ggctcgaatg
     4741 tcacgtacag ggtggcgtca atcagtctgt agttctgcat gaccaggccg ccctgtctca
     4801 gggccataat ttcggcatgg gctgttgggt cgtgcaggcc gatggctctg ttccagccct
     4861 cgccgatcac tctattgttc agcaccagca cggctcccac aggcacctcc ctctcatccc
     4921 gtgccctctt ggccagggtc agggcatgtc tcatccagta ctcgtgggaa aactccacct
     4981 cagagacttt ccgcttcttc tttggtgact cgaactcgct tccgtcggct gtccgtttca
     5041 tggtggctgt ggatcccggg ctgcaggaat tcagatctgc caaagttgag cgtttattct
     5101 gagcttctgc aaaaagaaca agcaagctta gctccagctt ttgttccctt tagtgagggt
     5161 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5221 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5281 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5341 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5401 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5461 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5521 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5581 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5641 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5701 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5761 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5821 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5881 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5941 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6001 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6061 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6121 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6181 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6241 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6301 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6361 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6421 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6481 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6541 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6601 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6661 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6721 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6781 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6841 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6901 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6961 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7021 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7081 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7141 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7201 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7261 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7321 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7681 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7741 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt
     7801 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7861 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7921 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
     7981 aattgggtac cggcc
//
