LOCUS       sequence-406989-        2461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator of acetamidase-encoding gene in A. niger..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB416
FEATURES             Location/Qualifiers
     source          1..2461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      398..455
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(479..496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(650..1238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(730..749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1421..1515)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1536..2195)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2110..2129
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2357..2375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2379..2422)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2425..2446
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcttctaata agtgtcagat agcaatttgc acaagaaatc aataccagca actgtaaata
       61 agcgctgaag tgaccatgcc atgctacgaa agagcagaaa aaaacctgcc gtagaaccga
      121 agagatatga cacgcttcca tctctcaaag gaagaatccc ttcagggttg cgtttccagt
      181 ctagacacgt ataacggcac aagtgtctct caccaaatgg gttatatctc aaatgtgatc
      241 taaggatgga aagcccagaa tattggctgg gttgatggct gcttcgagtg cagtctcatg
      301 ctgccacagg tgactctgga tggccccata ccactcaacc catggtacga gcggtacgct
      361 tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt
      421 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
      481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
      661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      841 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1021 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1081 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1141 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1201 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1261 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1381 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg
     1441 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1501 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg
     1561 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1621 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1681 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1741 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
     1801 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1861 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1921 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1981 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2041 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2101 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2161 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2221 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2281 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     2341 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     2401 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc
     2461 g
//
