LOCUS       sequence-407003-        9739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0859
FEATURES             Location/Qualifiers
     source          1..9739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1454..1483
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1457..1486
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    2677..3265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2730..2750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3148..3159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3337..3570
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3648..3769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3685..3704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3739..3758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3809..3944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3964..3983)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3965..3983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3993..4010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3993..4009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4002..4024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4151..4606
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4238..4257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4448..4469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4632..4736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4737..4805,4806..5597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4955..4974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5768..6356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6257..6276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6510..6527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6644..6665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6680..6685,6686..6703,6704..6710)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6718..6734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6723..6745
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6742..6758
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6742..6758
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6777..6797
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6779..6797
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6825..7051
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7052..7232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7279..7404
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7897..8130
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8315..8359
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8590..8800
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8612..8808
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8659..8794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8840..9442
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8840..8859)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9336..9356
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9485..9602
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgac
     1201 gatgctcctg gacggaggcc cgcagttccc tgggctggga gtgggcagct tcggcgcgcc
     1261 gcgccaccac gagatgccca accgtgagcc ggcaggcatg gggctgaatc ccttcgggga
     1321 ctcaacccac gccgccgccg ccgccgccgc cgccgctgcc ttcaagctga gccctgccgc
     1381 ggcgcacgat ctatcttcag gccagagctc ggctttcacg ccgcagggtt cgggctacgc
     1441 caacgccctg ggccaccatc accaccacca tcaccatcat caccacacca gccaggtgcc
     1501 cagctacggt ggcgctgcct ctgccgcctt caactcaacg cgcgagtttc tgttccgcca
     1561 gcgcagctcc gggctcagtg aggcggcctc gggtggcggg cagcacgggc tcttcgccgg
     1621 ctcggcgagc agcctgcatg ctccagctgg catccccgag ccccctagct acttgctgtt
     1681 tcccgggctg catgagcagg gcgctgggca cccgtcgccc acagggcacg tggacaacaa
     1741 ccaggtccac ctggggctgc gtggggagct gttcggccgt gctgacccat accgcccagt
     1801 ggccagcccg cgcacggacc cctacgcggc cggcgctcag tttcctaact acagccccat
     1861 gaacatgaac atgggagtga acgtggcggc ccaccacggg cccggcgcct tcttccgtta
     1921 tatgcggcag cctatcaagc aggagctgtc gtgcaagtgg atcgacgagg ctcagctgag
     1981 ccggcccaag aagagctgcg accggacctt cagcaccatg catgagctgg tgacacatgt
     2041 caccatggag catgtggggg gcccggagca gaacaaccac gtctgctact gggaggagtg
     2101 cccccgggag ggcaagtctt tcaaggcgaa gtacaaactg gtcaaccaca tccgagtgca
     2161 cacgggcgag aagcccttcc catgcccctt cccgggctgc gggaagatct ttgcccgttc
     2221 tgagaacctc aagatccaca agaggaccca cacaggtgag aaacctttca aatgtgaatt
     2281 tgaaggctgt gacagacgct ttgccaacag cagcgaccgt aagaagcaca tgcatgtgca
     2341 tacctcggac aagccctata tctgcaaagt gtgcgacaag tcctacacgc acccgagctc
     2401 cctgcgcaaa cacatgaagt gttgtcctgc ttggtatccg ggacagtctc taattcctga
     2461 cgaagaactt gatactgacg ttggtatgca gcagccagcc ctccataaca ctacctatcc
     2521 taaatgcagg gttaatgccg aacctactgt gcaagaaatg atttactagt aatgaactag
     2581 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaca
     2641 cgatgtaacg gacaggtagt aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     2701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3061 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3181 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3241 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3301 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3361 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3421 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3481 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3541 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3601 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3661 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3721 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3781 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3961 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4021 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4081 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4141 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4561 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5281 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5881 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6421 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6481 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6541 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6601 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6661 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6721 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6781 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6841 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6901 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6961 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7021 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7201 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7261 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7321 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7381 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7441 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7501 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7561 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7621 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7681 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7741 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7801 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7861 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7921 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7981 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8041 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8101 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8161 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8221 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     8281 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8341 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8401 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8461 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8521 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8581 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     8641 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8701 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8761 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8821 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8881 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8941 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9001 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     9061 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9121 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9181 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9241 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9301 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9361 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9421 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     9481 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9541 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9601 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     9661 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     9721 cttgaaagga gtgggaatt
//
