LOCUS       sequence-407006-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0619
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4435..5023
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4488..4508)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4906..4917)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5095..5328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5406..5527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5443..5462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5497..5516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5567..5702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5722..5741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5723..5741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5751..5768)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5751..5767)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5760..5782)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5909..6364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5996..6015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6206..6227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6390..6494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6495..6563,6564..7355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6713..6732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7526..8114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8015..8034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8268..8285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8402..8423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8438..8443,8444..8461,8462..8468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8476..8492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8481..8503
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8500..8516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8500..8516
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8535..8555
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8537..8555
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8583..8809
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8810..8990
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9037..9162
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9655..9888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10073..10117
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10348..10558
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10370..10566
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10417..10552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10598..11200
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10598..10617)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11094..11114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11243..11360
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 tatggccctg gaattgcaag cccaggcatc tccgcagcca gagcctgaag aactcctgat
     1261 tgtgaaactg gaagaggact cttggggatc agaatccaaa ctctgggaga aggaccgtgg
     1321 ctctgtctct ggcccagagg cctcccgcca gcgcttcagg caattccaat acagggatgc
     1381 agctggaccc cacgaggcct tcagccagct ctgggctctc tgctgtcgtt ggctgaggcc
     1441 ggagatccgt ctcaaagagc agatcctgga gctgctcgtg ctggagcagt tcctgactat
     1501 cttgcctagg gaggtccaga cctgggtgca ggcacgccac cctgagagtg gtgaggaggc
     1561 tgtggccttg gtggaggatt ggcaccgaga gaccaggact gcaggacagt cgggactgga
     1621 attgcataca gaagagacca ggcccttaaa gacaggggaa gaagctcaga gcttccagct
     1681 gcagccagtg gatccctggc ctgagggaca gtcccagaag aagggggtga agaatacatg
     1741 ccctgacctt cccaatcacc taaatgccga ggtggcacca cagcctttga aagagagtgc
     1801 tgtcctcact ccccgagtcc ctactctccc aaagatgggg agcgttggag attgggaggt
     1861 gacagctgag tcccaggaag ccctgggccc tggcaaacat gctgagaagg agctctgtaa
     1921 agacccccca ggagacgact gtgggaacag cgtgtgcctg ggagttccag tttcaaaacc
     1981 aagtaatacc tccgagaaag agcaaggacc agagttttgg ggtctaagtc ttataaattc
     2041 tgggaaaagg agcactgcag attacagcct ggataatgag ccagctcagg cattgacctg
     2101 gagggattca agagcctggg aggaacaata ccagtgggat gtggaggaca tgaaggtgtc
     2161 aggtgttcac tggggctatg aggagaccaa gactttcctg gcaattttga gtgaatctcc
     2221 tttctctgaa aagctccgga cttgtcacca gaaccgccag gtatatcggg ccattgcaga
     2281 gcagctaagg gcaaggggct tcctgcggac actggagcaa tgtcgctata gggtcaaaaa
     2341 cctcctacgg aattaccgga aagccaagag cagccaccca ccaggtacct gccccttcta
     2401 tgaggagctg gaggccctgg tcagggctcg gacagccatc agagccacag atggcccagg
     2461 agaggccgtg gcacttccca ggctcgggta tagtgacgca gagatggatg agcaggagga
     2521 agggggctgg gatcctgaag aaatggcaga agactgtaac ggtgctggcc tggtcaatgt
     2581 tgagtctacc caggggccca ggattgcagg ggccccagct ctgttccaga gtcgtattgc
     2641 aggtgtgcac tggggctatg aggagaccaa ggccttcctg gcaattctca gtgagtcccc
     2701 attctcggaa aagcttcgta cctgtcacca gaacagccag gtgtaccggg ccattgcaga
     2761 gcggctgtgt gctctgggct tcctgcggac actggagcag tgtcgctaca gattcaaaaa
     2821 cctccttcga agctaccgga aagccaagag cagccaccca ccagggacat gccctttcta
     2881 tgaggaactg gactcgctga tgagggctcg ggctgcagtc agggccatgg ggactgtccg
     2941 agaggctgca ggtctcccta ggtgtgggca gagtagtgct gagactgatg cccaggaggc
     3001 ctggggtgaa gtggccaatg aagatgctgt caaaccttca accttgtgtc ctaaagcccc
     3061 agacatgggt tttgaaatga ggcatgagga tgaagaccag atttcagagc aggacatttt
     3121 tgagggtttg cctggagcct tatcaaaatg tcctacagaa gctgtttgcc aacctcttga
     3181 ctggggagaa gacagtgaaa atgaaaatga agatgaaggg cagtggggaa atccctcaca
     3241 ggaacagtgg caagaaagtt cttctgaaga ggacttagaa aaacttattg accatcaagg
     3301 cctgtacctt gcagagaaac cctacaagtg tgacacatgc atgaagagct tcagtcggag
     3361 ctcccacttc attgcccatc agcgaatcca cacaggtgag aagccctaca aatgccttga
     3421 atgtggaaaa aactttagtg accgctctaa cctcaatacc catcagagaa tccacactgg
     3481 agagaagccc tataaatgcc ttgaatgtgg gaaaagcttt agtgaccatt ctaatctcat
     3541 cactcaccag agaattcaca cgggggaaaa gccctataaa tgtggagaat gttggaaaag
     3601 cttcaaccag agctcaaacc ttctgaaaca tcagagaatc cacttgggag gaaatcctga
     3661 ccagtgtagt gagcctgggg gaaactttgc ccaaagccca tcttttagtg ctcactggag
     3721 gaattctaca gaagagacag ctcctgaaca acctcaaagt atcagtaagg acttgaattc
     3781 tcctggacca cacagcacaa actcagggga gaaactttat gagtgttctg aatgtggaag
     3841 aagcttctct aagagctctg ccctcattag tcaccaaaga atccatacgg gagagaaacc
     3901 atatgaatgt gccgaatgtg ggaaaagctt cagtaagagc tccaccctgg ccaaccacca
     3961 gcgcacccac actggagaga agccgtataa atgtgtggac tgtgggaagt gcttcagtga
     4021 gcgctccaag ctcatcacac accagagagt gcacacagga gagaagccct acaaatgcct
     4081 tgagtgtgga aaattcttcc gtgaccgttc taacctcatt actcaccaga ggattcatac
     4141 gggagagaag ccgtataagt gcagagagtg tgggaaatgc tttaaccaga gctccagtct
     4201 tattattcac cagagaatcc acacagggga gaaaccctac aagtgcacag agtgtggcaa
     4261 agacttcaac aacagttccc acttcagtgc tcaccggaga acccatgcag gagggaaggc
     4321 gtcgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     4381 ttgtggaaag gacgaaagtt acgagctgtc tttccaataa cgcgttaagt cgacaatcaa
     4441 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4501 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4561 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4621 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4681 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4741 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4801 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4861 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4921 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4981 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     5041 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5101 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5161 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5221 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5281 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     5341 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5401 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5461 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5521 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     5581 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5641 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5701 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     5761 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5821 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5881 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5941 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6001 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6061 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6121 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6181 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6241 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6301 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6361 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8401 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8461 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8521 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     8581 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     8641 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     8701 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     8761 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     8821 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8881 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8941 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9001 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9061 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     9121 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     9181 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9241 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     9301 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     9361 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     9421 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     9481 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9541 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9601 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9661 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9721 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9781 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9841 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9901 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9961 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10021 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    10081 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    10141 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    10201 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10261 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
    10321 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
    10381 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    10441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    10501 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    10561 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
    10621 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
    10681 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
    10741 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
    10801 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
    10861 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
    10921 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
    10981 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
    11041 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
    11101 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    11161 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
    11221 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    11281 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    11341 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    11401 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    11461 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
