LOCUS       sequence-407011-        9880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2966
FEATURES             Location/Qualifiers
     source          1..9880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1367..1391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1399..1440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1551..2139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1604..1624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2022..2033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2211..2444
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2522..2643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2559..2578)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2613..2632
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2683..2818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2838..2857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2839..2857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2867..2884)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2867..2883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2876..2898)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3025..3480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3112..3131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3322..3343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3506..3610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3611..3679,3680..4471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3829..3848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5131..5150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5384..5401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5518..5539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5554..5559,5560..5577,5578..5584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5592..5608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5597..5619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5616..5632
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5616..5632
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5651..5671
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5653..5671
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5699..5925
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5926..6106
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6153..6278
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6771..7004
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7189..7233
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7464..7674
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7486..7682
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7533..7668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7714..8316
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7714..7733)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8210..8230
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8359..8476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8614..9792
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8845..9783
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9740..9760
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    9812..9836
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgcttatga gcgcgaagca aacgcgcgcg gctcctgagg ctccagagac gcggctggtg
       61 gatcagtgct ggggctgttc gtgcggcgat gagcccgggg tgggcagaga ggggctgctg
      121 ggcgggcgga acgtggcgct cctgtaccgc tgctgctttt gcggtaaaga ccacccacgg
      181 cagggcagca tcctctacag catgctgacg agcgcaaagc aaacgtacgc ggcaccgaag
      241 gcgcccgagg cgacgctggg tccgtgctgg ggctgttcgt gcggctctga tcccggggtg
      301 ggcagagcgg ggcttccggg tgggcggccc gtggcactcc tgtaccgctg ctgcttttgt
      361 ggtgaagacc acccgcggca gggcagcatc ctctacagct tgctcactag ctcaaagcaa
      421 acgcacgtgg ctccggcagc gcccgaggca cggccagggg gcgcgtggtg ggaccgctcc
      481 tacttcgcgc agaggccagg gggtaaagag gcgctaccag gcgggcgggc cacggcgctt
      541 ctgtaccgct gctgcttttg cggtgaagac cacccgcagc agggcagcac cctctactgc
      601 gtgcccacga gcacaaatca agcgcaggcg gctccggagg agcggccgag ggccccctgg
      661 tgggacacct cctctggtgc gctgcggccg gtggcgctca agagtccaca ggtggtctgc
      721 gaggcagcct cagcgggcct gttgaagacg ctgcgcttcg tcaagtactt gccctgcttc
      781 caggtgctgc ccctggacca gcagctggtg ctggtgcgca actgctgggc gtccctgctc
      841 atgcttgagc tggcccagga ccgcttgcag ttcgagactg tggaagtctc ggagcccagc
      901 atgctgcaga agatcctcac caccaggcgg cgggagaccg ggggcaacga gccactgccc
      961 gtgcccacgc tgcagcacca tttggcaccg ccggcggagg ccaggaaggt gccctccgcc
     1021 tcccaggtcc aagccatcaa gtgctttctt tccaaatgct ggagtctgaa catcagtacc
     1081 aaggagtacg cctacctcaa ggggaccgtg ctctttaacc cggacgtgcc gggcctgcag
     1141 tgcgtgaagt acattcaggg actccagtgg ggaactcagc aaatactcag tgaacacacc
     1201 aggatgacgc accaagggcc ccatgacaga ttcatcgaac ttaatagtac ccttttcctg
     1261 ctgagattca tcaatgccaa tgtcattgct gaactgttct tcaggcccat catcggcaca
     1321 gtcagcatgg atgatatgat gctggaaatg ctctgtacaa agatataccc aactttcttg
     1381 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1441 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1501 ggaaaggacg aaacattccg accatcctac cggaaacgcg ttaagtcgac aatcaacctc
     1561 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1621 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     1681 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     1741 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     1801 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     1861 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     1921 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     1981 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2041 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2101 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     2161 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     2221 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     2281 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2341 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2401 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     2461 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     2521 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2581 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2641 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     2701 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2761 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2821 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2881 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2941 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3001 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3061 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3421 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3841 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4141 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5401 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5461 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5521 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5581 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5641 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     5701 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     5761 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     5821 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     5881 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     5941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     6121 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     6181 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     6241 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     6301 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     6361 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     6421 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     6481 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     6541 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     6601 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     6661 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     6721 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     6781 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     6841 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     6901 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     6961 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     7021 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     7081 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     7141 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     7201 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     7261 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     7321 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     7381 acccacctcc caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt
     7441 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt
     7501 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7561 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7621 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7681 aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc
     7741 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     7801 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     7861 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     7921 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     7981 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     8041 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     8101 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     8161 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     8221 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     8281 acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat
     8341 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     8401 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     8461 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     8521 cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     8581 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     8641 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     8701 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     8761 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     8821 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     8881 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     8941 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     9001 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     9061 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     9121 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     9181 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     9241 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     9301 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     9361 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     9421 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     9481 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     9541 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     9601 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     9661 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     9721 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     9781 caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag ttggtatggc
     9841 gggcgagaac caccagtggc agggcagcat cctctacaac
//
