LOCUS       sequence-407041-       10262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Delta-TK-FLAG
FEATURES             Location/Qualifiers
     source          1..10262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2917..2940)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2983..3002)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2984..3002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3047..3250)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3076..3096)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3251..3630
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3642..4097)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3779..3800)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3991..4010
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4124..4228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4229..4297,4298..5089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4447..4466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        5149..5506
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5149..5340
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5172..5192)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5357..5492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5541..6335
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5541..6335
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5595..5614)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6205..6224
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6523..6542)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6567..6614
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6943..7531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7432..7451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7663..7887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7876..7893
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        7913..7931
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7913..7930
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaaaaggttg gggtcatttt cactgggtcc agcgagaagg ttttccttgg agttccaacg
       61 agcggcttca ctcagaccct gaggctcaaa gtcagatgct actggccgct gaagggcttt
      121 tgaactctgc ttaaatccag tggctgagtg gacgatgaca ttcagaaacc catagagccc
      181 cggagactca tcatcttcct tattgatggt cagcggaatg ctgtggacag tctggagttt
      241 cacacacgag ttggtcagca tctgcagctc cacggatgtc agggagaagc ttctgaaaca
      301 cttcttctgc tgctcccgga tgttctccct ccactctgca cgctcatagt cagaggagat
      361 caggaacgtg taactcttgc cgttgcggct gtgcaccctg aaggccatgc tgggagacat
      421 aagcagcagc agtgactcct gctccgacag cttcttcttc agcctctccg tagccttgct
      481 gcccttgttc gccctcttct ctctctggat gtcactcttg atctgggaga tcttgatctt
      541 caaagcgtcc agctcctcat cgggcaccag ggggatgttg ggcactgcct ccagttcatc
      601 caccatctgg aagctgagat ccgtgagcgg aatgtaccat ttgcagtcat actgctgcgt
      661 tttgcctccg ctctgcttct tgagcttggt gcagagaagc aggtcggtga acaggaagac
      721 gtggcgcagc ttgcgggccc cctccaccag ctccaccatg aagctgtcct tcagcagctg
      781 ccggtgctct cccttcttca ccgtcatgga ctgccgtcgg ggtgtgatct cctcattgat
      841 gctggacagg aagttctgtg agatgcggag ggcgtcctgc agcaaggggt ggtcagggtg
      901 gctggcagga gtgtgcttca gcaagtcatg gaggaccagc gtgctcctcg tcacacggtc
      961 cacaggcttg tagagcagag tttccagaga gttcttggtc gttggatcct tggcatcttt
     1021 gttgcttctg gctctcaggt tctcggagat ttctgcaaac tgagcattgg cctgacagca
     1081 cttctcagcc atttccatgg caactccgta gttgtccacg aaggcccggt acacacccag
     1141 ctggctggcc agcttctgga agaggtcgcc cacccgctgc tggtggctcc actgctgcac
     1201 gcgggggaag agcccatcat agaactcctt gtggatctcg tagagctcag gcactttgaa
     1261 gaagatggtc tcgatctgct gactcgtcag caccggctga gaggtggtgg cagcggcttt
     1321 caaaggcttc atgggcagca gcagtgcctc caggtggctc aggtaagtct cctcgctagc
     1381 caggattccc gacaggaccc attttctcat ctccaagccc ttttccaagt ccagctcact
     1441 cgctttagtg gactccaggg ctcccgagac cagcgcatcc cggctgcccc tgcccttgct
     1501 gctgaggtgg ggcgatgagg agggcgagtc atcaatgtag ggcagcccat cttggtgccg
     1561 gcgctgctcc tctgcccggt ctgcagcgca gccgtatcca ggtggtgttc cgaacgagcc
     1621 atctgcgtct ccatggaagg cgccctcgcc atcgttgggc cagatctgcc cggtcttgcg
     1681 gacgcccacg atggtggcct cggacacgac aaccgggcag tgccggtgcc gcttatggca
     1741 ctgcggcgtg gggggactgc tgctgtcgaa ggactgttgc gagttctgcg agggagagcg
     1801 gcttttgtcc cggaacatgc ggtaggtggt ggggcttggg gacacgcggc tggactggcc
     1861 agaggagaag tcctcctcgc tggaggtgag gttctcattg gagctgcagt ccggggtata
     1921 gccgcctccg caatcctcaa aactccgggg ggagtaggac ctgcggggcc aggtaaggcg
     1981 cttctcctgc tcagaggtgc tctggctgcg caggagcggg cccttgccct ccccttccat
     2041 catgcccccg acgtagatgc tctggtaggg ctggtactcc aggggcggcc aagggggcct
     2101 gctaccgcca ttggcgtcga tcaggttgtc cttcaggaag cgggggttca actcggcgtc
     2161 ctcgtagtcg ccgtcgacgc cgcagctgct ctccgaggag cgtccccggt aagggggcct
     2221 ggatgcatcc cccacgctcg agcccgcgcc gtgctgggac tttttgcgct ccatctgcat
     2281 ggcctggctg cccagggagc tgatgcggtc cgacacctct ttgtcgttga ccttcaccag
     2341 gccgcgctcg tggtgaaact cgacgttcac gtagaagggc ttctcggcgt ccgccccggg
     2401 ctggccatgg cccttgcgta tccgctcgaa gttggacctg agcgccgcca cgctggcggg
     2461 gggtccccgg tcgtcccgtt cccccgacgc ggctgccccg ggcctgcggg cggtcccggg
     2521 cctggcctta cccggagaac cctcgccgtc gggccgggcc tcgggctcct cggcgggcgg
     2581 cgggtcggct ccgtcggcgg gcgctggctg cgggcgcgac gcggacgctc ggggctcgga
     2641 ggcgccgtcg ggggcctgcg ccgcgcgccg gaagccccat cgctgccggt catagctctt
     2701 cttttccttg gccagcaacg tctgcaggta gatcatgcgg aagcgctcct ggttcacctc
     2761 ctgctccagg cgccgaatgg aggccttgca gcgctccagc tcctgctcga tgtcgcccac
     2821 tgagcgcagc tccatgcgcg ggggctctga gtccgggaac tgcgccttcc acgcctccgc
     2881 gaagcccacc gggtccacca gatatctgca gaattcttta tcgtcatcgt ctttgtaatc
     2941 catgttttga ttcccggggg atccggtacc aagcttgggt ctccctatag tgagtcgtat
     3001 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
     3061 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     3121 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     3181 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     3241 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     3301 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     3361 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     3421 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     3481 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     3541 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     3601 actattaata actagtcaat aatcaatgtc aacgcgcgcg taaattgtaa gcgttaatat
     3661 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3721 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3781 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3841 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3901 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3961 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4021 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4081 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4441 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4741 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5101 agtttactca tatatacttt agattgattt attgaaaaag gaagagtcct gaggcggaaa
     5161 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     5521 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5581 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5641 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5701 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     5761 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5821 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5881 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5941 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6001 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6061 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6121 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6181 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6241 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6301 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     6361 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     6421 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6481 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     6541 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     6601 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     6661 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     6721 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     6781 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7621 ggataaccgt attaccgcca ctggttcttt ccgcctcaga agccatagag cccaccgcat
     7681 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     7741 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     7801 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     7861 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta gcatttaggt
     7921 gacactatag aatagggccc tctagactag tcatctactc gagcggccgc ctacctctgc
     7981 actatgtcac tgatttcctt caccgaactg aggagcttgc tgaagtcctg agtggccgct
     8041 ggaccactgc ctgctgtcgc cgggcagatc tgaagctccc ggagattatt ctccagtttg
     8101 ttgatggcct ctcggaaggc aaacttgttc ctcatttgct ggatggaatc cacatagctc
     8161 acgcagaacg tgtagaggtt tttgccggcc tccagcactg cgctgtggct ggccatctgc
     8221 tcggagttcc tagagatggc gaggcacagc gcctcggtgc tgtccaggac cacgcccttg
     8281 gtgatggcgc cgctggcgat ccgctctgga ggctggcggg ttttccgaag agacactcgg
     8341 gttgatatga gagggatgaa ggcggtggaa gacggctggt cccctgccag ggccgaggat
     8401 gctgatggca acgtggaggg aacgggggct gggctgatgg gggtccccga cggcttggcg
     8461 gactgtggct ttggagtggc cgggagcacg ggctttttga ggccctctcc cggctggctg
     8521 ggcttggcag cgtcactgtt cacagcatca accagactcg tggcttttgt ctttgcgccc
     8581 aggactgcct ccccggccgc ctcctggctc gggctctgcg agggctttcc tccagccttc
     8641 cctgcagagg ctgctggtgg gggcggcggg gcaggtttga gcctggacaa tttccccttg
     8701 tccctccctg gcgactcaga ggagtgcttg tgcctcctca ctctggactc ctcggcgggg
     8761 cccttgctgg tgccccctgg tgcacctgag cctgctttgc tggtgggggt cactggctca
     8821 gctgcagcag gggtccctaa ggcactgccc tttccagctt cttccttgtg ggggaggccc
     8881 gaggcagggg ccacggtgac ctgccgccgg aggggttttg gagtcaggtt gggcgggctg
     8941 gagcccgggc tggactccat gatgtctttg aagacctcat cagcagcttc ctcattcttt
     9001 ttcaccagcc tggggggagg cgttactgtg cctcgggtca cctggtcaga cctgttctcc
     9061 cctgccctct tccgaggcag agccggcttc tcacttttgt gccctccaaa tgtggacgag
     9121 tcaaactgtc ttcccgtgga ctgcaagtcc cgaggcagcg tgactgacct ccactccgtg
     9181 tccttggccc catggggaac gcaggaggcg gagcaagagc gcaggaagcg cttgctggag
     9241 ctgccaccgc cctcctcctc gccggtggct aggcggctgc tggtcagcgt gctggacttc
     9301 ttccacaggt ggggagaccg gaagcctgag cccccagact cccggagggc tccattgggg
     9361 accccagccc cattgctggg ctttggggac ttggctgggt cagctgtgtc caagggggtg
     9421 aaagccagtg ccccgttgct gatgtctcgg ccctcttcct cgccggcccc tctgcgctcc
     9481 ggctggccgt ccatctcccg gaaggagctg ctgcgtttgg gaggggttgg ggctgtcttc
     9541 ttcttcttct tgatcaaggc gctgaacaag ttggtctttt tgtctttggg gagaaggcgc
     9601 tcatcttcat tcaggccgcc ctccggggga cctcgctctt ttcgagggag caatggagac
     9661 acggcaggct catggtccag aggatcgctc tctccctggc ccttggagtg aggcatctca
     9721 ggcacgtcag tggtgtctct gtgctctgca gctctcctgg aggtcctcgt cttggtcaca
     9781 ccatagacag tgggcttgtt gcgctttggg gctggataat ggagcgtggt gatgagcccg
     9841 tcggccaccg ttgaatgatg atgaaccaac tcggccaggg tgttgaagcg gctctcggag
     9901 gagacgtaga gcttgccatc agaagcagtg ttgatcctgt aatggtacac cctcccttcg
     9961 tatctcagcg agatggacct ctggccagga ctgctctcac tctcacgcac caagaagctg
    10021 ccattgatcc cgctgctcag cagatactca gcggcattgc gggacacagg cccatggtac
    10081 caggagtgtt tctccagact gttgactggc gtgatgtagt tgcttgggac ccagccttgg
    10141 ccatttttgg tttgggcttc acaccattcc ccattgtgat tatagcctaa gacccggagc
    10201 ttttcacctt tagttatgct tagagtgtta tctccactgg ccacaaaatc atacagtgca
    10261 ac
//
