LOCUS       sequence-407042-       12356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the A NIMPLY B gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ
FEATURES             Location/Qualifiers
     source          1..12356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    911..944
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      945..1652
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1653..1686
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1753..1941
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2969..3143
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3531..5183
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3597..3615)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5203..5403
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5746..5801
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5809..5829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6513..6701
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7325..7499
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7569..7590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7605..7610,7611..7628,7629..7635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7643..7659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7648..7670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7667..7683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7667..7683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7679..8002
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7696..7752
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7753..7770)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7753..7769)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7762..7784)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7978..7997)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8134..8158
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8246..8956
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8977..9081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9082..9876
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10047..10635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10536..10555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10971..12119
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12265..12289
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gggttgctta agaataagta attcttaagc aacctcgaca
       61 aaatttagaa cgaacttaat tatgatctca aatacattga tacatatctc atctagatct
      121 aggttatcat tatgtaagaa agttttgacg aatatggcac gacaaaatgg ctagactcga
      181 tgtaattggt atctcaactc aacattatac ttataccaaa cattagttag acaaaattta
      241 aacaactatt ttttatgtat gcaagagtca gcatatgtat aattgattca gaatcgtttt
      301 gacgagttcg gatgtagtag tagccattat ttaatgtaca tactaatcgt gaatagtgaa
      361 tatgatgaaa cattgtatct tattgtataa atatccataa acacatcatg aaagacactt
      421 tctttcacgg tctgaattaa ttatgataca attctaatag aaaacgaatt aaattacgtt
      481 gaattgtatg aaatctaatt gaacaagcca accacgacga cgactaacgt tgcctggatt
      541 gactcggttt aagttaacca ctaaaaaaac ggagctgtca tgtaacacgc ggatcgagca
      601 ggtcacagtc atgaagccat caaagcaaaa gaactaatcc aagggctgag atgattaatt
      661 agtttaaaaa ttagttaaca cgagggaaaa ggctgtctga cagccaggtc acgttatctt
      721 tacctgtggt cgaaatgatt cgtgtctgtc gattttaatt atttttttga aaggccgaaa
      781 ataaagttgt aagagataaa cccgcctata taaattcata tattttcctc tccgctttga
      841 aggttgctta agaataagta attcttaagc aacctactgt atttttacaa caattaccaa
      901 caacaacaaa gaagttccta ttctctagaa agtataggaa cttcctgctt taatgagata
      961 tgcgagaagc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
     1021 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
     1081 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
     1141 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
     1201 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
     1261 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
     1321 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     1381 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     1441 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     1501 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     1561 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     1621 gaattttgcg acaacatgtc gaggctcagc aggaagttcc tattctctag aaagtatagg
     1681 aacttccaac aaacaacatt acaattacta tttacaatta caatgacttc aaaggtgtat
     1741 gatcctgagc aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     1801 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     1861 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     1921 ccaaaatttg ttgatgtgca gagaaaaaga atgataactg gtccacaatg gtgggcaaga
     1981 tgtaaacaga tgaacgtgct tgattctttc attaattact acgattcaga aaagcacgct
     2041 gagaatgcag ttatctttct tcacggaaac gctgcatctt catatttgtg gagacatgtt
     2101 gtgcctcaca ttgaaccagt ggctaggtgt attatcccag atcttatcgg aatgggaaag
     2161 tctggtaaat ctggaaatgg ttcttacaga cttttggatc attataagta cctcacagct
     2221 tggttcgaac ttctcaactt acctaagaag attattttcg ttggacacga ttggggtgct
     2281 tgcttggcat tccattactc atacgaacac caagataaga tcaaagctat agttcatgca
     2341 gagagtgttg tggatgtgat agaatcttgg gatgagtggc cagatattga agaggatatt
     2401 gctttgatca agtctgaaga gggagaaaag atggttctcg aaaataactt tttcgtggaa
     2461 actatgcttc cttcaaagat tatgagaaaa ttggaaccag aagagtttgc tgcatatctc
     2521 gaacctttca aggaaaaagg agaggttaga aggccaacac tcagttggcc tagggaaata
     2581 ccattagtta agggaggtaa acctgatgtt gtgcagatcg tgagaaatta taacgcttac
     2641 cttagggcat cagatgattt gcctaagatg ttcatcgagt cagatccagg atttttcagt
     2701 aatgctatag ttgaaggtgc aaagaaattt ccaaacaccg agttcgttaa ggtgaaagga
     2761 ttgcatttct cacaagaaga tgctcctgat gagatgggaa agtatattaa gtcttttgtt
     2821 gaaagagtgc tcaaaaacga gcagggttct cgatctgatg cttctgaagt aacgtcaaca
     2881 agctcccagt ctgaggtatg cacagtggaa acgccaggtt gtgttggttc ttaagcttct
     2941 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     3001 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     3061 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     3121 aatccagtac taaaatccag atcgctgcaa gaattcaagc ttggagccaa cactcgaatc
     3181 cccacccgtt ggaccaaacc cggctcatta agcgtcggtt cagatttatt tcctttattt
     3241 aaaaaaaagg aaagggtaaa aaatagaaaa ttggaaacag ttaaagccca aaattgtaat
     3301 ttaccgagaa ttgtaaattt acctgaaaac cctacgctat agtttcgact ataaatacca
     3361 aacttaggac ctcacttcag aatcccctcg tcgctgcgtc tctctcccgc aaccttcgat
     3421 tttcgtttat tcgcatccat cggagagaga aaacaatcaa taagcgacct actacaaaca
     3481 agtaagtaag agaaaaacca aaagaagaag agaaacaaca agaagaagta atggaggacg
     3541 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     3601 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     3661 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     3721 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     3781 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     3841 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     3901 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3961 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     4021 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     4081 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     4141 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     4201 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     4261 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     4321 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     4381 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     4441 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     4501 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     4561 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     4621 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     4681 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     4741 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     4801 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     4861 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     4921 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     4981 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     5041 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     5101 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     5161 agggcggaaa gtccaaattg taagcttgat cccccgtcga cagctagcta tatcatcaat
     5221 ttatgtatta cacataatat cgcactcagt ctttcatcta cggcaatgta ccagctgata
     5281 taatcagtta ttgaaatatt tctgaattta aacttgcatc aataaattta tgtttttgct
     5341 tggactataa tacctgactt gttattttat caataaatat ttaaactata tttctttcaa
     5401 gatcgctact agaattcgag ctcggagcca acactcgaat ccccacccgt tggaccaaac
     5461 ccggctcatt aagcgtcggt tcagatttat ttcctttatt taaaaaaaag gaaagggtaa
     5521 aaaatagaaa attggaaaca gttaaagccc aaaattgtaa tttaccgaga attgtaaatt
     5581 tacctgaaaa ccctacgcta tagtttcgac tataaatacc aaacttagga cctcacttca
     5641 gaatcccctc gtcgctgcgt ctctctcccg caaccttcga ttttcgttta ttcgcatcca
     5701 tcggagagag aaaacaatca ataagcgacc tactgtattt ttacaacaat taccaacaac
     5761 aacaaacaac aaacaacatt acaattacta tttacaatta caatggctcc taagaagaag
     5821 aggaaggtga tgagccagtt cgacatcctg tgcaagaccc cccccaaggt gctggtgcgg
     5881 cagttcgtgg agagattcga gaggcccagc ggcgagaaga tcgccagctg tgccgccgag
     5941 ctgacctacc tgtgctggat gatcacccac aacggcaccg ccatcaagag ggccaccttc
     6001 atgagctaca acaccatcat cagcaacagc ctgagcttcg acatcgtgaa caagagcctg
     6061 cagttcaagt acaagaccca gaaggccacc atcctggagg ccagcctgaa gaagctgatc
     6121 cccgcctggg agttcaccat catcccttac aacggccaga agcaccagag cgacatcacc
     6181 gacatcgtgt ccagcctgca gctgcagttc gagagcagcg aggaggccga caagggcaac
     6241 agccacagca agaagatgct gaaggccctg ctgtccgagg gcgagagcat ctgggagatc
     6301 accgagaaga tcctgaacag cttcgagtac accagcaggt tcaccaagac caagaccctg
     6361 taccagttcc tgttcctggc cacattcatc aactgcggca ggttcagcga catcaagaac
     6421 gtggacccca agagcttcaa gctggtgcag aacaagtacc tgggcgtgat cattcagtgc
     6481 ctggtgaccg agactaagac aagcgtgtcc aggtaagttt ctgcttctac ctttgatata
     6541 tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata
     6601 aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt
     6661 ataacttttc taatatatga ccaaaatttg ttgatgtgca ggcacatcta ctttttcagc
     6721 gccagaggca ggatcgaccc cctggtgtac ctggacgagt tcctgaggaa cagcgagccc
     6781 gtgctgaaga gagtgaacag gaccggcaac agcagcagca acaagcagga gtaccagctg
     6841 ctgaaggaca acctggtgcg cagctacaac aaggccctga agaagaacgc cccctacccc
     6901 atcttcgcta tcaagaacgg ccctaagagc cacatcggca ggcacctgat gaccagcttt
     6961 ctgagcatga agggcctgac cgagctgaca aacgtggtgg gcaactggag cgacaagagg
     7021 gcctccgccg tggccaggac cacctacacc caccagatca ccgccatccc cgaccactac
     7081 ttcgccctgg tgtccaggta ctacgcctac gaccccatca gcaaggagat gatcgccctg
     7141 aaggacgaga ctaaccccat cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc
     7201 gagggcagca tcagataccc cgcctggaac ggcatcatca gccaggaggt gctggactac
     7261 ctgagcagct acatcaacag gcggatctga gcttctctag ctagagtcga tcgacaagct
     7321 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
     7381 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     7441 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg
     7501 ctttactgag accgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7561 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7621 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7681 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     7741 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7801 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7861 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     7921 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     7981 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8041 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga cggtctcagg
     8101 gacacgaagt gatccgttta aactatcagt gtttgacagg atatattggc gggtaaacct
     8161 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     8221 cgttcgtcca tttgtatgtg ccagccgcct ttgcgacgct caccgggctg gttgccctcg
     8281 ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt
     8341 gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg
     8401 acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag
     8461 atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg
     8521 aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg
     8581 ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga
     8641 cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt
     8701 ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac
     8761 ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg
     8821 ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg
     8881 cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc gaacggcctc
     8941 accccaaaaa tggcagcgct ggccaattcc cgagtgcgcg gaacccctat ttgtttattt
     9001 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9061 taatattgaa aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg
     9121 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     9181 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     9241 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     9301 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     9361 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     9421 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     9481 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     9541 gtggattgcg aaaactggga agaggacact ccatttaaag atccgcgcga gctgtatgat
     9601 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
     9661 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
     9721 agggcggaca agtggtatga cattgccttc tgcgtccggt cgctcaggga ggatatcggg
     9781 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
     9841 ataaaatatt atattttact ggatgaattg ttttagctgt cagaccaagt ttactcatat
     9901 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9961 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc agatcctaga
    10681 tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga cttcttccgc atcaagtgtt
    10741 ttggctctca ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc
    10801 agggcaagat tcggaatacc aagtacgaga aggacggcca gacggtctac gggaccgact
    10861 tcattgccga taaggtggat tatctggaca ccaaggcacc aggcgggtca aatcaggaat
    10921 aagggcacat tgccccggcg tgagtcgggg caatcccgca aggagggtga atgaatcgga
    10981 cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc gccgaggatg
    11041 ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg
    11101 gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc
    11161 ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg
    11221 caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc aagaagcgaa
    11281 aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga
    11341 aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt gcgccgtggc
    11401 cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc accacgcgca
    11461 acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg
    11521 tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg gtgtggcagc
    11581 aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc acgttctacg
    11641 agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag gccgaggaat
    11701 gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg
    11761 aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt
    11821 gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat
    11881 tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc gactatttca
    11941 gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg
    12001 attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag
    12061 gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc aaacgctagg
    12121 gccttgtggg gtcagttccg gctgggggtt cagcagcccc tgctcggatc tgttggaccg
    12181 gacagtagtc atggttgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
    12241 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
    12301 tagacaactt aataacacat tgcggacgtt tttaatgtac tggggttgaa cactct
//
