LOCUS       sequence-407043-        8531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmStaufen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
  TITLE     mRNA degradation by miRNAs and GW182 requires both CCR4:NOT 
            deadenylase and DCP1:DCP2 decapping complexes.
  JOURNAL   Genes Dev. 2006 Jul 15. 20(14):1885-98.
  PUBMED    16815998
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Jul 15. 20(14):1885-98."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmStaufen_B
FEATURES             Location/Qualifiers
     source          1..8531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2531..2596
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2639..2665
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             5251..5259
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5782..5823
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5833..5850
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5873..5890)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5923..6057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5947..5966)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6001..6020
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6658..7246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6738..6757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7417..8208,8209..8277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8040..8059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8278..8382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8450..8468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtacccaac atggacgcac aaacacgacg acgtgagcgt cgcgctgaga aacaagctca
     2581 atggaaagct gcaaacggtg gatctcctcc aagcttgaat tctgcagata tcatggctta
     2641 cccatacgat gttccagatt acgctatgca gcacaacgtt catgccgccc ggccagcgcc
     2701 ccacatccga gctgcgcacc atcacagcca cagccatgcc cacatgcatc tgcatcccgg
     2761 catggagcag cacctgggcc ccagtcttca gcagcaacag cagccgccac cgccgcccca
     2821 gcagcctcca catcgcgacc tccacgcacg tctgaatcat caccatctcc acgctcagca
     2881 gcagcagcag caacagacgt cgtcgaacca agccgccgcc gtcgctgcgg ctggggctgc
     2941 ctatcaccac ggcaacatca acagcaacag cggcagcaac atctccagca acagcaacca
     3001 gatgcagaag atccgccaac agcaccagca cttgagtagc agcaacggcc ttcttggcaa
     3061 tcaacctcct ggtccgccgc cacaggcctt caatccattg gcgggcaatc cggcagctct
     3121 cgcctacaat cagcttcctc cgcatccgcc gcaccacatg gccgcccatc tgggcagcta
     3181 tgccgctcca ccgcctcact attacatgag ccaggcgaag cccgcgaaat ataatcacta
     3241 tggcagcaat gcaaacagca acagcggcag caacaacagc aacagcaact atgcccccaa
     3301 ggccatactg cagaatacgt ataggaatca gaaggtggtg gttccaccag tggtgcagga
     3361 agtaaccccg gtgccggaac cgccagtgac caccaataat gccaccacca acagcaccag
     3421 caacagcaca gtcatagcta gcgagcctgt aacccaagag gatacttctc agaagccgga
     3481 gactagacag gagccagctt cagcggacga tcatgtcagc acggggaata tcgatgctac
     3541 gggagcactg tccaacgagg acacgagcag ttcaggacgc gggggcaagg acaagacccc
     3601 aatgtgcctg gtcaatgagt tggccaggta caacaagatc acgcatcaat atcggttgac
     3661 tgaagaacga ggacctgccc actgcaagac cttcacggtg acactgatgc tgggcgatga
     3721 ggagtactcg gcggatggtt ttaagattaa gaaagcgcaa catttggcgg ctagtaaggc
     3781 tatcgaggaa accatgtaca agcatccgcc gccgaagatt cgccgcagcg aagaaggcgg
     3841 tcccatgcgc acacacatta cgccgacggt ggaattgaat gccctggcca tgaagttggg
     3901 acagcgcacc ttctacctgc tggatcccac tcagattcct cccacggatt ccatagtgcc
     3961 gccggagttt gccggcggtc atttactaac tgcaccagga ccagggatgc cgcaaccacc
     4021 tccaccacct gcttacgctc tgcgccagcg gcttggcaat ggatttgtgc ccattccatc
     4081 gcagccgatg catccgcatt tctttcatgg acctggtcag cgaccgtttc ctccgaagtt
     4141 tccatcacgc tttgcgctgc caccgcctct cggagctcat gtacatcatg gaccaaatgg
     4201 cccatttcca tccgtgccta cgccacccag caaaatcacc ctgtttgtgg gcaagcagaa
     4261 gtttgtgggc atcggacgca cactgcagca agcgaaacat gacgctgcgg cgagagcgct
     4321 gcaggttctc aagacacagg ccatatctgc ctctgaggag gctctcgagg attcgatgga
     4381 tgagggtgac aagaagtcgc ccatctcgca ggttcacgag atcggaatta agcgcaacat
     4441 gaccgttcat tttaaggtgt tgcgcgagga ggggccggcg cacatgaaga actttataac
     4501 agcctgcatt gtgggatcta ttgtcaccga aggagaagga aatggcaaaa aagtgtccaa
     4561 gaagcgtgct gcggaaaaga tgctcgtcga gttgcaaaag ctgccgcccc ttacgcccac
     4621 caagcagacg ccattgaagc ggattaaggt taagacacca ggcaaaagtg gtgcagctgc
     4681 ccgagaaggt tcggttgtgt cgggcactga tggtactatg caaacgggaa agccggagag
     4741 acgtaaacgc ctcaatccgc cgaaggacaa gctgatagac atggacgatg cggataatcc
     4801 cattacaaag ctaattcaac tgcagcagac gcgtaaggaa aaggaaccca ttttcgagct
     4861 gatagccaaa aacggcaatg aaaccgctcg acgacgggaa ttcgttatgg aggtctccgc
     4921 cagcgggagc acagcccgtg gaacaggcaa cagcaagaaa ttggcaaagc gaaatgctgc
     4981 acaggctcta tttgaactgc tggaagctgt tcaagtcaca ccaaccaacg aaacacagtc
     5041 atcggaggaa tgctgcactt cggcaacaat gtccgccgtt acagcgcccg cagtggaggc
     5101 aactgcggaa ggcaaagtgc ccatggtagc aacgccagtg ggtcccatgc caggcatttt
     5161 aatactgcgc cagaacaaga aaccagcaaa gaagagggat caaattgtaa ttgtcaagtc
     5221 taacgtggaa tccaaagagg aggaggccaa taaggaggtg gcagttgcag ctgaggagaa
     5281 tagcaacaac agtgcaaact ctggcgacag cagcaacagc agcagcggcg attcgcaagc
     5341 cacagaggcg gctagtgaga gtgcactgaa tacctcaacc ggcagcaata cgagtggtgt
     5401 gagtagcaac agcagcaatg ttggagccaa cacggatggt aacaaccatg ccgagagtaa
     5461 aaacaacacg gagagtagca gcaacagtac gagtaacaca caaagcgctg gagtgcacat
     5521 gaaggagcag ctcttgtacc ttagtaaact gctggacttt gaggtcaact tctcggacta
     5581 tccgaaaggc aatcacaacg agttcctgac catcgtgaca ttgtccacac atccgccgca
     5641 gatttgccat ggcgttggca agagctccga ggagtcgcag aatgatgctg caagcaatgc
     5701 cttgaaaatc ctcagcaagc tggggctcaa caatgccatg aaataagcgg ccgctcgagt
     5761 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     5821 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     5881 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
     5941 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     6001 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     6061 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     6121 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt
     6181 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     6241 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     6301 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     6361 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     6421 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     6481 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     6541 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     6601 acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7081 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     7741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     8041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8401 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8461 cacgaggccc tttcgtcttc aagaattaat tctatattct aaaaacacaa atgatacttc
     8521 taaaaaaaaa t
//
