LOCUS       sequence-407045-        8370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of HsPABPc1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pETM-41-HsPABPC1_J
FEATURES             Location/Qualifiers
     source          1..8370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..1215
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1189..1212
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1267..1287
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3235..3252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3305..3323)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3319..3366
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3403..3858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3490..3509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3700..3721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3950..4765)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3950..4765)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4673..4692
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4757..4776
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4887..5475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5376..5395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5629..5646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5661..5800
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5726..5745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5886..5908
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5902..6093)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6902..7984)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7946..7965
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7985..8062)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8268..8287
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg aaacatcacc atcaccatca ccccatgaaa
      121 atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct
      181 gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat
      241 aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc
      301 tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg
      361 gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc
      421 aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg
      481 ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg
      541 aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt
      601 gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg
      661 ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac
      721 aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa
      781 acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat
      841 tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg
      901 ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa
      961 aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc
     1021 gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccactatg
     1081 gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat
     1141 gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg
     1201 aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacccc
     1261 atgagcgaga atctttattt tcagggcgcc atggacggta ccggatccat gaaccccagt
     1321 gcccccagct accccatggc ctcgctctac gtgggggacc tccaccccga cgtgaccgag
     1381 gcgatgctct acgagaagtt cagcccggcc gggcccatcc tctccatccg ggtctgcagg
     1441 gacatgatca cccgccgctc cttgggctac gcgtatgtga acttccagca gccggcggac
     1501 gcggagcgtg ctttggacac catgaatttt gatgttataa agggcaagcc agtacgcatc
     1561 atgtggtctc agcgtgatcc atcacttcgc aaaagtggag taggcaacat attcattaaa
     1621 aatctggaca aatccattga taataaagca ctgtatgata cattttctgc ttttggtaac
     1681 atcctttcat gtaaggtggt ttgtgatgaa aatggttcca agggctatgg atttgtacac
     1741 tttgagacgc aggaagcagc tgaaagagct attgaaaaaa tgaatggaat gctcctaaat
     1801 gatcgcaaag tatttgttgg acgatttaag tctcgtaaag aacgagaagc tgaacttgga
     1861 gctagggcaa aagaattcac caatgtttac atcaagaatt ttggagaaga catggatgat
     1921 gagcgcctta aggatctctt tggcaagttt gggcctgcct taagtgtgaa agtaatgact
     1981 gatgaaagtg gaaaatccaa aggatttgga tttgtaagct ttgaaaggca tgaagatgca
     2041 cagaaagctg tggatgagat gaacggaaag gagctcaatg gaaaacaaat ttatgttggt
     2101 cgagctcaga aaaaggtgga acggcagacg gaacttaagc gcaaatttga acagatgaaa
     2161 caagatagga tcaccagata ccagggtgtt aatctttatg tgaaaaatct tgatgatggt
     2221 attgatgatg aacgtctccg gaaagagttt tctccatttg gtacaatcac tagtgcaaag
     2281 gttatgatgg agggtggtcg cagcaaaggg tttggttttg tatgtttctc ctccccagaa
     2341 gaagccacta aagcagttac agaaatgaac ggtagaattg tggccacaaa gccattgtat
     2401 gtagctttag ctcagcgcaa agaagagcgc caggctcacc tcactaacca gtatatgcag
     2461 agaatggcaa gtgtacgagc tgttcccaac cctgtaatca acccctacca gccagcaccc
     2521 ccttcaggtt acttcatggc agctatccca cagactcaga accgtgctgc atactatcct
     2581 cctagccaaa ttgctcaact aagaccaagt cctcgctgga ctgctcaggg tgccagacct
     2641 catccattcc aaaatatgcc cggtgctatc cgcccagctg ctcctagacc accatttagt
     2701 actatgagac cagcttcttc acaggttcca cgagtcatgt caacacagcg tgttgctaac
     2761 acatcaacac agacaatggg tccacgtcct gcagctgcag ccgctgcagc tactcctgct
     2821 gtccgcaccg ttccacagta taaatatgct gcaggagttc gcaatcctca gcaacatctt
     2881 aatgcacagc cacaagttac aatgcaacag cctgctgttc atgtacaagg tcaggaacct
     2941 ttgactgctt ccatgttggc atctgcccct cctcaagagc aaaagcaaat gttgggtgaa
     3001 cggctgtttc ctcttattca agccatgcac cctactcttg ctggtaaaat cactggcatg
     3061 ttgttggaga ttgataattc agaacttctt catatgctcg agtctccaga gtcactccgt
     3121 tctaaggttg atgaagctgt agctgtacta caagcccacc aagctaaaga ggctgcccag
     3181 aaagcagtta acagtgccac cggtgttcca actgtttaag cggccgcact cgagcaccac
     3241 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     3301 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     3361 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
     3421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3841 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3901 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
     3961 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     4021 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     4081 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     4141 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     4201 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     4261 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     4321 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     4381 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     4441 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     4501 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     4561 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     4621 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     4681 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     4741 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     4801 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5521 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5581 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5641 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5701 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     5761 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     5821 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5881 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     5941 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     6001 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     6061 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     6121 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     6181 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     6241 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     6301 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     6361 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     6421 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     6481 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     6541 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     6601 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     6661 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     6721 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     6781 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     6841 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     6901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6961 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     7021 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     7081 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     7141 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     7201 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     7261 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     7321 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     7381 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     7441 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     7501 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     7561 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     7621 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     7681 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     7741 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     7801 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     7861 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     7921 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     7981 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     8041 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     8101 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     8161 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     8221 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     8281 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     8341 tagaggatcg agatctcgat cccgcgaaat
//
