LOCUS       sequence-407089-       12211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescence and bioluminescence expression in Gram-negative 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Ozdemir E, Abdelhamed H, Ozdemir O, Lawrence M, Karsi A
  TITLE     Development of Bioluminescent Virulent Aeromonas hydrophila for 
            Understanding Pathogenicity.
  JOURNAL   Pathogens. 2023 May 2;12(5):670. doi: 10.3390/pathogens12050670.
  PUBMED    37242340
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pathogens."; date: "2023-05-2"; volume: "12(5):670. doi"; pages: " 
            10.3390/pathogens12050670"
COMMENT     Sequence Label: pAKgfplux3
FEATURES             Location/Qualifiers
     source          1..12211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             705..1817
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(1873..2532)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDSRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2447..2466
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(2576..2595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2577..2595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2605..2622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2605..2621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2614..2636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(3330..3992)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      3993..4764
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(join(6089..6880,6881..6949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6712..6731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6950..7021)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    7249..7270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7285..7290,7291..7308,7309..7315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7323..7339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7328..7350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7347..7363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7347..7363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7382..7402
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7384..7402
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             7456..8169
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7486..7514)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8109..8129
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             8230..9672
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             9685..10608
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             10657..11739
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             join(11754..12211,1..526)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
ORIGIN
        1 ccctaaaata tctgtaaatc cccatgctta tacgccaggc ggacctcgga aatatgtaac
       61 agcaaccagt catcatattg ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa
      121 gtgggatgat tctaatgatg ttagatatga atatgctgaa agatataaag ccgttgcgga
      181 taaatatgac gttgacctat cagagataga ccatcagtta atgatattag ttaactataa
      241 cgaagatagt aataaagcta aacaagagac gcgtgcattt attagtgatt atgttcttga
      301 aatgcaccct aatgaaaatt tcgaaaataa acttgaagaa ataattgcag aaaacgctgt
      361 cggaaattat acggagtgta taactgcggc taagttggca attgaaaagt gtggtgcgaa
      421 aagtgtattg ctgtcctttg aaccaatgaa tgatttgatg agccaaaaaa atgtaatcaa
      481 tattgttgat gataatatta agaagtacca catggaatat acctaataga tttcgagttg
      541 cagcgaggcg gcaagtgaac gaatccccag gagcatagat aactatgtga ctggggtgag
      601 tgaaagcagc caacaaagca gcagcttgaa agatgaaggg tataaaagag tatgacagca
      661 gtgctgccat actttctaat attatcttga ggagtaaaac aggtatgact tcatatgttg
      721 ataaacaaga aattacagca agctcagaaa ttgatgattt gattttttcg agcgatccat
      781 tagtgtggtc ttacgacgag caggaaaaaa tcagaaagaa acttgtgctt gatgcatttc
      841 gtaatcatta taaacattgt cgagaatatc gtcactactg tcaggcacac aaagtagatg
      901 acaatattac ggaaattgat gacatacctg tattcccaac atcggttttt aagtttactc
      961 gcttattaac ttctcaggaa aacgagattg aaagttggtt taccagtagc ggcacgaatg
     1021 gtttaaaaag tcaggtggcg cgtgacagat taagtattga gagactctta ggctctgtga
     1081 gttatggcat gaaatatgtt ggtagttggt ttgatcatca aatagaatta gtcaatttgg
     1141 gaccagatag atttaatgct cataatattt ggtttaaata tgttatgagt ttggtggaat
     1201 tgttatatcc tacgacattt accgtaacag aagaacgaat agattttgtt aaaacattga
     1261 atagtcttga acgaataaaa aatcaaggga aagatctttg tcttattggt tcgccatact
     1321 ttatttattt actctgccat tatatgaaag ataaaaaaat ctcattttct ggagataaaa
     1381 gcctttatat cataaccgga ggcggctgga aaagttacga aaaagaatct ctgaaacgtg
     1441 atgatttcaa tcatctttta tttgatactt tcaatctcag tgatattagt cagatccgag
     1501 atatatttaa tcaagttgaa ctcaacactt gtttctttga ggatgaaatg cagcgtaaac
     1561 atgttccgcc gtgggtatat gcgcgagcgc ttgatcctga aacgttgaaa cctgtacctg
     1621 atggaacgcc ggggttgatg agttatatgg atgcgtcagc aaccagttat ccagcattta
     1681 ttgttaccga tgatgtcggg ataattagca gagaatatgg taagtatccc ggcgtgctcg
     1741 ttgaaatttt acgtcgcgtc aatacgagga cgcagaaagg gtgtgcttta agcttaaccg
     1801 aagcgtttga tagttgatga cctgcagccc gggggatcca ctagtagggc accaataact
     1861 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1921 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1981 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     2041 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     2101 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2161 cacatcttgc gaatatatgt gtagaaactg ccgggaatcg tcgtggtatt cactccagag
     2221 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2281 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     2341 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2401 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2461 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2521 ttttttctcc attttagctt ccttagctcc tgaaaatctc gagagctcca attcgcccta
     2581 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2641 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2701 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2761 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2821 ttttttaacc aataggccgt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     2881 cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat
     2941 gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa
     3001 atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc
     3061 gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt
     3121 gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa tccgttagcg
     3181 aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg
     3241 cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc tggaacagcg
     3301 cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc
     3361 ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc
     3421 tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc
     3481 ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc
     3541 ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg
     3601 tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg
     3661 ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc
     3721 acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa
     3781 gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc
     3841 atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg
     3901 gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct
     3961 tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc
     4021 acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc
     4081 gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg
     4141 gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct
     4201 ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt
     4261 cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg
     4321 atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac
     4381 tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg cccttcgact gcccccactc
     4441 gcataggctt gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga
     4501 gccttgaccc gccttccact tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc
     4561 ggaggctttt ccccagagaa aattaaaaaa attgatgggg caaggccgca ggccgcgcag
     4621 ttggagccgg tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa
     4681 gctcgtgggc aggcgcagcc tgtccatcag cttgtccagc agggttgtcc acgggccgag
     4741 cgaagcgagc cagccggtgg ccgctcgcgg ccatcgtcca catatccacg ggctggcaag
     4801 ggagcgcagc gaccgcgcag ggcgaagccc ggagagcaag cccgtagggc gccgcagccg
     4861 ccgtaggcgg tcacgacttt gcgaagcaaa gtctagtgag tatactcaag cattgagtgg
     4921 cccgccggag gcaccgcctt gcgctgcccc cgtcgagccg gttggacacc aaaagggagg
     4981 ggcaggcatg gcggcatacg cgatcatgcg atgcaagaag ctggcgaaaa tgggcaacgt
     5041 ggcggccagt ctcaagcacg cctaccgcga gcgcgagacg cccaacgctg acgccagcag
     5101 gacgccagag aacgagcact gggcggccag cagcaccgat gaagcgatgg gccgactgcg
     5161 cgagttgctg ccagagaagc ggcgcaagga cgctgtgttg gcggtcgagt acgtcatgac
     5221 ggccagcccg gaatggtgga agtcggccag ccaagaacag caggcggcgt tcttcgagaa
     5281 ggcgcacaag tggctggcgg acaagtacgg ggcggatcgc atcgtgacgg ccagcatcca
     5341 ccgtgacgaa accagcccgc acatgaccgc gttcgtggtg ccgctgacgc aggacggcag
     5401 gctgtcggcc aaggagttca tcggcaacaa agcgcagatg acccgcgacc agaccacgtt
     5461 tgcggccgct gtggccgatc tagggctgca acggggcatc gagggcagca aggcacgtca
     5521 cacgcgcatt caggcgttct acgaggccct ggagcggcca ccagtgggcc acgtcaccat
     5581 cagcccgcaa gcggtcgagc cacgcgccta tgcaccgcag ggattggccg aaaagctggg
     5641 aatctcaaag cgcgttgaga cgccggaagc cgtggccgac cggctgacaa aagcggttcg
     5701 gcaggggtat gagcctgccc tacaggccgc cgcaggagcg cgtgagatgc gcaagaaggc
     5761 cgatcaagcc caagagacgg cccgagacct tcgggagcgc ctgaagcccg ttctggacgc
     5821 cctggggccg ttgaatcggg atatgcaggc caaggccgcc gcgatcatca aggccgtggg
     5881 cgaaaagctg ctgacggaac agcgggaagt ccagcgccag aaacaggccc agcgccagca
     5941 ggaacgcggg cgcgcacatt tccccgaaaa gtgccacctg gattttggtc atgagattat
     6001 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6061 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6121 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6181 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6241 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6301 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6361 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6421 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6481 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6541 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6601 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6661 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6721 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6781 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6841 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6901 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6961 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7021 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     7081 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtttttatg
     7141 catgcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7201 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7261 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7321 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     7381 cgcaattaac cctcactaaa gggaacaaaa gctgggtacc cggggatcct ctagatttaa
     7441 gaaggagata tacatatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     7501 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     7561 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     7621 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     7681 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     7741 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     7801 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     7861 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     7921 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     7981 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     8041 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     8101 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     8161 ctatacaaat aaatgtccag acctgcaggc atgcaagctt gatatcgaat tcaggcttgg
     8221 aggatacgta tgactaaaaa aatttcattc attattaacg gccaggttga aatctttccc
     8281 gaaggtgatg atttagtgca atccattaat tttggtgata atagtgttta cctgccaata
     8341 ttgaatgact ctcatgtaaa aaacattatt gattgtaatg gaaataacga attacggttg
     8401 cataacattg tcaattttct ctatacggta gggcaaagat ggaaaaatga agaatactca
     8461 agacgcagga catacattcg tgacttaaaa aaatatatgg gatattcaga agaaatggct
     8521 aagctagagg ccaattggat atctatgatt ttatgttcta aaggcggcct ttatgatgtt
     8581 gtagaaaatg aacttggttc tcgccatatc atggatgaat ggctacctca ggatgaaagt
     8641 tatgttcggg cttttccgaa aggtaaatct gtacatctgt tggcaggtaa tgttccatta
     8701 tctgggatca tgtctatatt acgcgcaatt ttaactaaga atcagtgtat tataaaaaca
     8761 tcgtcaaccg atccttttac cgctaatgca ttagcgttaa gttttattga tgtagaccct
     8821 aatcatccga taacgcgctc tttatctgtt atatattggc cccaccaagg tgatacatca
     8881 ctcgcaaaag aaattatgca acatgcggat gttattgtcg cttggggagg gccagatgcg
     8941 attaattggg cggtagagca tgcgccatct tatgctgatg tgattaaatt tggttctaaa
     9001 aagagtcttt gcattatcga taatcctgtt gatttgacgt ccgcagcgac aggtgcggct
     9061 catgatgttt gtttttacga tcagcgagct tgtttttctg cccaaaacat atattacatg
     9121 ggaaatcatt atgaggaatt taagttagcg ttgatagaaa aacttaatct atatgcgcat
     9181 atattaccga atgccaaaaa agattttgat gaaaaggcgg cctattcttt agttcaaaaa
     9241 gaaagcttgt ttgctggatt aaaagtagag gtggatattc atcaacgttg gatgattatt
     9301 gagtcaaatg caggtgtgga atttaatcaa ccacttggca gatgtgtgta ccttcatcac
     9361 gtcgataata ttgagcaaat attgccttat gttcaaaaaa ataagacgca aaccatatct
     9421 atttttcctt gggagtcatc atttaaatat cgagatgcgt tagcattaaa aggtgcggaa
     9481 aggattgtag aagcaggaat gaataacata tttcgagttg gtggatctca tgacggaatg
     9541 agaccgttgc aacgattagt gacatatatt tctcatgaaa ggccatctaa ctatacggct
     9601 aaggatgttg cggttgaaat agaacagact cgattcctgg aagaagataa gttccttgta
     9661 tttgtcccat aataggtaaa aagtatggaa aatgaatcaa aatataaaac catcgaccac
     9721 gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc agaagaaaac
     9781 agcccaaaga gaaagaatgc cattattatt gcgtctggtt ttgcccgcag gatggatcat
     9841 tttgctggtc tggcggaata tttatcgcgg aatggatttc atgtgatccg ctatgattcg
     9901 cttcaccacg ttggattgag ttcagggaca attgatgaat ttacaatgtc tataggaaag
     9961 cagagcttgt tagcagtggt tgattggtta actacacgaa aaataaataa cttcggtatg
    10021 ttggcttcaa gcttatctgc gcggatagct tatgcaagcc tatctgaaat caatgcttcg
    10081 tttttaatca ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag agctttaggg
    10141 tttgattatc tcagtctacc cattaatgaa ttgccgaata atctagattt tgaaggccat
    10201 aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt ttggttggga agatttagct
    10261 tctacaatta ataacatgat gtatcttgat ataccgttta ttgcttttac tgcaaataac
    10321 gataattggg tcaagcaaga tgaagttatc acattgttat caaatattcg tagtaatcga
    10381 tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt agtggtcctg
    10441 cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga tcatctggat
    10501 attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac agtcaatgaa
    10561 cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa tctattgaga
    10621 tattctatca ctcaaatagc aatataagga ctctctatga aatttggaaa ctttttgctt
    10681 acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt taaattaggt
    10741 cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca tttcacggag
    10801 tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc gactaaaaaa
    10861 ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg ccaacttgaa
    10921 gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat ttgccgaggg
    10981 ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg cgccttagcg
    11041 gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga agctgataat
    11101 gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag aggtggcgca
    11161 ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca atttggccta
    11221 ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact tgagctttat
    11281 aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg cttatcatat
    11341 ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa atttctgggg
    11401 cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga ccaaacaaga
    11461 ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca taaagatact
    11521 aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca ggaatgtatt
    11581 gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg tggatttgaa
    11641 gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc tgatgtcatg
    11701 ccatttctta aagaaaaaca acgttcgcta ttatattagc taaggagaaa gaaatgaaat
    11761 ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa agtatagttc
    11821 gcatgcagga aataacggag tatgttgata agttgaattt tgaacagatt ttagtgtatg
    11881 aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct ggttttctgc
    11941 tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca actcatcatc
    12001 ctgtccgcat agcggaggaa gcttgcttat tggatcagtt aagtgaaggg agatttattt
    12061 tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc ccggttgaat
    12121 atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta acaacaggct
    12181 attgtaatcc agataacgat ttttatagct t
//
