LOCUS       sequence-407093-        7061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Cavbeta1 calcium channel
            subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7061)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CavB1/pFLAG-myc-CMV
FEATURES             Location/Qualifiers
     source          1..7061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(846..870)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(883..912)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(925..948)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          993..1819
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     primer_bind     complement(1934..1958)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1955..2158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1984..2004)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2159..2538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3104..3121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3104..3120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3113..3135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3367..3386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3486..3508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3546..3564)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(3774..3842,3843..4634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3992..4011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4805..5393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5294..5313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5547..5564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(5716..5721,5722..5739,5740..5746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5754..5770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5759..5781
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5778..5794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5778..5794
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5817..5951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5841..5860)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5895..5914
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    6088..6112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gttgaggacc gagcgcttgg ccagggaaat gtcagccgtt acccgggtga tggaaatcct
       61 gccatcaaac cgatgcttca agaagtcaaa caacgccttc tgcatcatgt cagttacctc
      121 atagcccttg agcgatggtc ccaccaggat gatgggcctc atggaaggca ccacgtcata
      181 ggggggcacg tgctctgtcg acttctgctt ctgtttggca ctggcggggg gtgtggggcg
      241 gcgggtgcca gtcaccacat ctcccagact ggaactggag ttgtcacctg acttgctgga
      301 gctgaggcgg ttttggcgca gcgtctgttc ctgcagcagg cgaaggctgt ccagtttgac
      361 agggctgggg atgaaaccaa cctcacagcc ttccttcacc agcctcccga tccaccagtc
      421 attattgtat ttctccttga tgtgcaggaa gtccttgggc tcaaaggtga tggccactcc
      481 ctgcactggc acctcatctc ctggagacgg attgtagcca acatttgtcc gaacagcaaa
      541 ggccactggt ttggtcttgg ctttctcgag ctgggctaag gcctggcgct ctgcctcctt
      601 ccttaaggct tcccggtcct cctccaggga cacgtcggag tctgacggcc ggctcgtgta
      661 ggactctgct gagccctgac ggacaaagct gttggatgtg gtatctgagg aggtgctccc
      721 atctgacctt ttgaaccgcc ctttcctctt gctgtatttg ccctgtgggc tggggtcgaa
      781 gacctccata gggatctctt gggaaggtgg gtaagggccc cgggacatgc cgctcttctg
      841 gacgaagcct gcttttttgt acaaacttgt tgattcgact ctcagatcct cttcagagat
      901 gagtttctgc tcgagatctc gcgatttatc gtcatcgtcc ttgtagtcca tggtggtacc
      961 tatcccggga agcttcgtgt caaggacggt gactgcagaa aagacccatg gaaaggaaca
     1021 gtctgttagt ctgtcagcta ttatgtctgg tggcgcgcgc ggcagcaacg agtactgctc
     1081 agactacact gccctccacc gttaacagca ccgcaacggg agttacctct gactcttatc
     1141 agaacacaac aactcagctg cctgcatctt cttctgccgc tgccttaagt cttccaaatg
     1201 cgtcagcggt gcaagcccgc tccccgagct cattttcaga cacataccct accgccacgg
     1261 ccttgtgcgg cacactggtg gtggtgggca tcgtgctgtg cctaagtctg gcctccactg
     1321 ttaggagcaa ggagctgccg agcgaccatg agtcgctgga ggcatgggag cagggctcgg
     1381 atgtagaagc tccgccgcta ccggagaaga gcccatgtcc ggaacacgta cccgagattc
     1441 gcgtggagat cccacgttat gtttaataaa aactgcgggc actggggacg gtggtgttgt
     1501 atatgtgaat ttgtaaataa taaatgagac cccatcctgt aaaaatacag agtccgtgtc
     1561 agtctctgaa ggacagtgta ttggcatata gccaataaag agagttgtgg caaagagcca
     1621 tgttatggat tagtaatgga aagtatcgtc accaataggg gagtggtcaa taatggtcaa
     1681 taacccacac ctataggcta agctatacca tcacctataa catgaggaag cgggggtgta
     1741 tagaccccaa gccaaaaaca gtatagcatg cataagaagc caagggggtg ggcctataga
     1801 ctctataggc ggtacttacg tcactcttgg cacggggaat ccgcgttcca atgcaccgtt
     1861 cccggccgcg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat
     1921 ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag acctcccacc
     1981 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     2041 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     2101 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     2161 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     2221 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     2281 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     2341 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     2401 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     2461 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     2521 tagtcaataa tcaatgtcaa catggcggta atgttggaca tgagccaata taaatgtaca
     2581 tattatgata tggatacaac gtatgcaatg gccaatagcc aatattgatt tatgctatat
     2641 aaccaatgaa taatatggct aatggccaat attgattcaa tgtatagatc gatatgcatt
     2701 ggccatgtgc cagcttgatg tcgcctctat cggcgatata gcctcatatc gtctgtcacc
     2761 tatatcgaaa ctgcgatatt tgcgacacac agaatcgccc aagtcaccaa agtcgtctat
     2821 cgccatcccc cgtaaacgat ataagcgcta tcgccagata tcgcgtatgc ccaaaaatca
     2881 cttttggaaa aatggcgata tcagttacac agaaactcac atcggcgaca ttttcaatat
     2941 gccatatttt caaatatcga tttttccaat atcgccatct ctatcggcga taaacaccac
     3001 tatcgcgcga catgaattta gtcggcgaca gaaatctcaa aacgcgtatt tcggacaaac
     3061 acacatttta ttattcactg cagtcaccgt ccttgacacg ggcactggcc gtcgttttac
     3121 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3181 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3241 gcagcctgaa tggcgaatgg cgccctgact aatttttttt atttatgcag aggccgaggc
     3301 cgcctcggcc tctgagctat tccagaagta gtgaggaggc gacgtcttca tatggtgcac
     3361 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3541 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3601 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3661 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3721 actcgagact agtggccggc atggccgcgg ccgcttattg aaaaaggaag agtatgagta
     3781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5641 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtagtt agctcactca
     5701 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     5761 cggataacaa tttcacacag gaaacagcta tgacatgatt acgcgagctc ggtacggatc
     5821 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     5881 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     5941 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     6001 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcaat
     6061 gaattcgata tcctgcaggt cgaatcaacc actttgtaca agaaagctgg gtcctagcgg
     6121 atgtagacgc cttgtcccca gccctccagc tcattcttat tgcgccccag aaccggccca
     6181 ccaccctccg cacagtagcg ggccttattc cggccccggt tcctgttgtc ggtcatctcc
     6241 tcctcataat cttcctcctc ttcccaggag ccctgccgag ctggtggtgt acctccccct
     6301 gcagggtctc caggccctgg gccctctgag gggtctgtct ccatgtccac acacgagtct
     6361 cctggctccg tgtaggcaga gtttcggctg ccgggggtgt cagcatcaaa ggtgtcttgg
     6421 cgggatagcg cccgcagggt gcctgcccgg ccaggtgggt ggttgctggg gtaaaggcct
     6481 gggggctggc ccagctcccc ggggtactcg tgcacactgg catgctcccc agtggcccgg
     6541 tccagcggct ggtccccgga agcaaggtag ggtccctgga ggttggagac gggggcaggg
     6601 ctggcagcca gagcggcggt agccatggtg cggttcagca gcggattggg tggcgtgctg
     6661 ctaggcgggt gtgtggcctt ccagtaggct tccaagtact ccgccaggtg ctcgcaggca
     6721 tcttccaatt ggttctcgtc caggattatg tcaaacattt cggggggaca ctgtgccagc
     6781 ttctccgagg ctgctatttg gacattgagg tgtttggatt gagacttccc tcgggatttg
     6841 atgagcctct gcagtacctt gggggatgtg atcttgatgt aaacaatgat gggggccagc
     6901 gaggttttag agagctgggc tgggtggttg atggtgtcgg cgtccagggc aaccagctgc
     6961 aaggtccggg ccagctcgaa gatccgctcg atttcactct gtacctcagc caggctggag
     7021 cgagtgttgg agcgctcgat gataatgtgt ttgctggggt t
//
