LOCUS       sequence-407096-        7669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red fluorescent marker DsRed-Express in Listeria 
            monocytogenes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Andersen JB, Roldgaard BB, Lindner AB, Christensen BB, Licht TR
  TITLE     Construction of a multiple fluorescence labelling system for use in 
            co-invasion studies of Listeria monocytogenes.
  JOURNAL   BMC Microbiol. 2006 Oct 3;6:86. doi: 10.1186/1471-2180-6-86.
  PUBMED    17014739
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-10-3"; volume: "6:86. doi"; pages: " 
            10.1186/1471-2180-6-86"
COMMENT     Sequence Label: PJEBAN6
FEATURES             Location/Qualifiers
     source          1..7669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            4491..4600
                     /note="incP origin of transfer"
     CDS             4633..5004
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     5315..5337
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5329..5346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5330..5346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5368..6045)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(5430..5453)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5628..5647)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5845..5865
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5897..5915
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6520..6536)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6520..6536)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6533..6555)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6544..6560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6568..6574,6575..6592,6593..6598))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6613..6634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6751..6768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6922..7510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7002..7021)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
       61 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      121 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      181 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcaatt ctatttaatc
      241 actttgacta gcaaatacta acaacaagac acacacacca aaaatcaaaa attcactact
      301 tttagttaaa aaccacgtaa ccacaagaac taatccaatc catgtaatcg ggttcttcaa
      361 atatttctcc aagattttcc tcctctaata tgctcaactt aaatgaccta ttcaataaat
      421 ctattatgct gctaaatagt ttataggaca aataagtata ctctaatgac ctataaaaga
      481 tagaaaatta aaaaatcaag tgttcgcttc gctctcactg cccctcgacg ttttagtagc
      541 ctttccctca cttcgttcag tccaagccaa ctaaaagttt tcgggctact ctctccttct
      601 ccccctaata attaattaaa atcttactct gtatatttct gctaatcatt cgctaaacag
      661 caaagaaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      721 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgttttaggg cgttctgcta
      781 gcttgtttaa agtctcttga atgaatgtat gctctaagtc aaaagaattt gtcagcgcct
      841 ttatatagct ttctttttct tcttttttta ctttaatgat cgatagcaac aatgatttaa
      901 cactagcaag ttgaatgcca ccatttcttc ctggtttaat cttaaagaaa atttcctgat
      961 tcgccttcag taccttcagc aatttatcta atgtccgttc aggaatgcct agcacttctc
     1021 taatctcttt tttggtcgtc actaaataag gcttgtatac atcgcttttt tcgctaatat
     1081 aagccattaa atcttctttc cattctgaca aatgaacacg ttgacgttcg cttctttttt
     1141 tcttgaattt aaaccaccct tgacggacaa ataaatcttt actggttaaa tcacttgata
     1201 cccaagcttt gcaaagaatg gtaatgtatt ccctattagc cccttgatag ttttctgaat
     1261 aggcacttct aacaattttg attacttctt tttcttctaa gggttgatct aatcgattat
     1321 taaactcaaa catattatat tcgcacgttt cgattgaata gcctgaacta aagtaggcta
     1381 aagagagggt aaacatgacg ttattacgcc ctattaaacc cttttctcct gaaaatttcg
     1441 tttcgtgcaa taagagatta aaccagggtt catctacttg ttttttgcct tctgtaccgc
     1501 ttaaaaccgt tagacttgaa cgagtaaagc ccttattatc tgtttgtttg aaagaccaat
     1561 cttgccattc tttgaaagaa taacggtaat taggatcaaa aaattctaca ttgtccgttc
     1621 ttggtatgcg agcaatacca aaatgattac acgttagatc aactggcaaa gactttccaa
     1681 aatattctcg gatattttgc gaaattattt tggctgcttt gacagattta aattctgatt
     1741 ttgaagtcac atagactggc gtttctaaaa caaaatatgc ttgataacct ttatcagatt
     1801 tgataatcat agtaggcata aaacctaaat caatagcggt tgttaaaata tcgcttgctg
     1861 aaatagtttc ttttgccgtg tgaatatcaa aatcaataaa gaaggtattg atttgtctta
     1921 aattgttttc agaatgtcct ttcgtgtatg aacggttttc gtctgcatac gttccataac
     1981 gataaacgtt gggtgtccaa tgtgtaaatg tatcttgatt ttcttgaatc gcttcctcgg
     2041 aagtcagaac aacaccacga ccgccaatca tgcttgattt tgagcgatac gcaaaaatag
     2101 cccctttgct tttacctggc ttggtagtga ttgagcgaat tttactattt ttaaatttgt
     2161 actttaacaa gccgtcatga agcacagttt ctacaacaaa agggatattc attcagctgt
     2221 tctcctttcc tataaatcct ataaaatagg ttgtttaatt aacttggttt gctttttcat
     2281 tcaactgttt caatattgca tgttttgaaa aagatttttt tcctttataa gtcaattttt
     2341 ttccactaat cgaataaatt attttgttat tttctattaa cttatatata taatcttccc
     2401 cctccgaaga aaaatactta tctgattttg tttctaagta gatatttctc ttttctaact
     2461 ctttcttaaa cgtttctagt gtatagatat ttgctaattt tcttatctcc aataaactat
     2521 tttttatata agttttacat tcatcatgat tcatacaaac tccaccttct ataaatgaat
     2581 acaaaaaaag caatcaaacg atttccgatt gattgcttaa caattcttaa attcagtagc
     2641 ttagatactt gaaaactctc tgatttccct atataatgat agtacggtta tataccgtct
     2701 tcaaacaaag ttaattaaat aacttcttac gagggaagag ttcatctgac taactgataa
     2761 gcgttggttt ggcaatctta tcgggctatg catttataaa atgtcgtcaa acattttata
     2821 aatgtgtcat ggctcttttt tcgtttctat tcagttcgtt gtttcgttat atctagtata
     2881 ccgcttttaa aaaaaantaa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     2941 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     3001 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     3061 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     3121 tactcatttc taattcttgg gagaattttt cttttgattt gcccaaattc cccgtaggcg
     3181 ctagggacct ctttagcttc ttggaagctg tcagtagtat atctaataat ttatctccat
     3241 tccctttagt aacgtgtaac tttccaaatt taaaaaagcg actcatagaa ttatttcctc
     3301 ccgttaaata atagataact attaaaaata gacaatactt gctcataagt aatggtactt
     3361 aaattgttta ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga
     3421 cgatattctc gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct
     3481 ggaacatctg tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga
     3541 tgaaagcatt ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc
     3601 aaccctagtg ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga
     3661 tagatgtcag acgcatggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg
     3721 taaggaatac tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata
     3781 tcttggtgaa ttaaagtgac acgaatgttc agttttaatt tttctgacga taagttgaat
     3841 agatgactgt ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt
     3901 aaatgccctt tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attcaattgt
     3961 tttattattt ggttgagtac tttttcactc gttaaaaagt tttgagaata ttttatattt
     4021 ttgttcatgt aattactcct gaagtgatta catctgtaaa taaatacaga agttaaacga
     4081 tttgtttgta attttagtta tctgtttaaa aagtcataag attagtcact ggtaggaatt
     4141 aatctaacgt atttatttat ctgcgtaatc actgttttta gtctgtttca aaacagtaga
     4201 tgttttatct acattacgca tttggaatac caacatgacg aatccctcct tcttaattac
     4261 aaatttttag catctaattt aacttcaatt cctattatac acaaaatttt aagatactgc
     4321 actatcaaca cactcttaag tttgcttcgg gggatctttt ccgctgcata accctgcttc
     4381 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
     4441 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
     4501 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
     4561 gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat
     4621 gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag
     4681 gtgtactgcc ttccagacga acgaagagcg attgaggaaa aggcggcggc ggccggcatg
     4741 agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac
     4801 tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg
     4861 ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc
     4921 gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg
     4981 gtccgcccga gggcagagcc atgacttttt tagccgctaa aacggccggg gggtgcgcgt
     5041 gattgccaag cacgtcccca tgcgctccat caagaagagc gacttcgcgg agctggtgaa
     5101 gtacatcacc gacgagcaag gcaagaccga tcccctatgc ggtgtgaaat accgcacaga
     5161 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     5221 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5281 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     5341 gccagtgcca agcttgcatg cctgcagtta caggaacagg tggtggcggc cctcggcgcg
     5401 ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggagtc
     5461 cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg acttgaactc
     5521 caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc ccttcagcac
     5581 gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatagtct tcttctgcat
     5641 tacggggccg tcggagggga agttcacgcc gatgaacttc accttgtaga tgaaggagcc
     5701 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
     5761 gcgctcccac ttgaagccct cggggaagga cagcttcttg tagtcgggga tgtcggcggg
     5821 gtgcttcacg tacaccttgg agccgtactg gaactggggg gacaggatgt cccaggcgaa
     5881 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     5941 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgcgcac
     6001 cttgaagcgc atgaactcct tgatgacgtc ctcggaggag gccattttaa taaacctcct
     6061 tcggatccgt tgtaaatgaa tttccacctt cagggttttt gaaaccataa aaccaaagca
     6121 atccaagcaa aatagctagg tagaaaaaag ttttcattgt aaaaatagtt gctggatgat
     6181 gcaaaattag ttttatttta ttcataaatc cacctcttca ttgtttcatt agtgttacaa
     6241 atattaaaaa taaacgttta atacttactt actgtaatac atttgtcttt aaatgtctaa
     6301 ttacattttt gtaaggttag aaaaataaac atattttgaa cttgaaagta ggtacgagta
     6361 taatgaaaca atagatatag caatgtttta atgctagaaa agaggttgga ctatgattaa
     6421 tcaaaagaag attgcaggag aaaaggcttg tgaatggatt aaagatggaa tggttgtggg
     6481 tcttggaacg gggggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
     6541 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6601 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6721 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6901 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7321 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7441 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7501 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7561 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7621 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaact
//
