LOCUS       sequence-407097-        7678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red fluorescent marker HcRed in Listeria monocytogenes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Andersen JB, Roldgaard BB, Lindner AB, Christensen BB, Licht TR
  TITLE     Construction of a multiple fluorescence labelling system for use in 
            co-invasion studies of Listeria monocytogenes.
  JOURNAL   BMC Microbiol. 2006 Oct 3;6:86. doi: 10.1186/1471-2180-6-86.
  PUBMED    17014739
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-10-3"; volume: "6:86. doi"; pages: " 
            10.1186/1471-2180-6-86"
COMMENT     Sequence Label: PJEBAN4
FEATURES             Location/Qualifiers
     source          1..7678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            4496..4605
                     /note="incP origin of transfer"
     CDS             4638..5009
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     5320..5342
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5334..5351
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5335..5351
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5373..6059)
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     primer_bind     complement(6535..6551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6535..6551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6548..6570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6559..6575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6583..6589,6590..6607,6608..6613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6628..6649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6766..6783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6937..7525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7017..7036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
       61 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      121 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      181 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcaattctat
      241 ttaatcactt tgactagcaa atactaacaa caagacacac acaccaaaaa tcaaaaattc
      301 actactttta gttaaaaacc acgtaaccac aagaactaat ccaatccatg taatcgggtt
      361 cttcaaatat ttctccaaga ttttcctcct ctaatatgct caacttaaat gacctattca
      421 ataaatctat tatgctgcta aatagtttat aggacaaata agtatactct aatgacctat
      481 aaaagataga aaattaaaaa atcaagtgtt cgcttcgctc tcactgcccc tcgacgtttt
      541 agtagccttt ccctcacttc gttcagtcca agccaactaa aagttttcgg gctactctct
      601 ccttctcccc ctaataatta attaaaatct tactctgtat atttctgcta atcattcgct
      661 aaacagcaaa gaaaaaacaa acacgtatca tagatataaa tgtaatggca tagtgcgggt
      721 tttattttca gcctgtatca tagctaaaca aatcgagttg tgtgtccgtt ttagggcgtt
      781 ctgctagctt gtttaaagtc tcttgaatga atgtatgctc taagtcaaaa gaatttgtca
      841 gcgcctttat atagctttct ttttcttctt tttttacttt aatgatcgat agcaacaatg
      901 atttaacact agcaagttga atgccaccat ttcttcctgg tttaatctta aagaaaattt
      961 cctgattcgc cttcagtacc ttcagcaatt tatctaatgt ccgttcagga atgcctagca
     1021 cttctctaat ctcttttttg gtcgtcacta aataaggctt gtatacatcg cttttttcgc
     1081 taatataagc cattaaatct tctttccatt ctgacaaatg aacacgttga cgttcgcttc
     1141 tttttttctt gaatttaaac cacccttgac ggacaaataa atctttactg gttaaatcac
     1201 ttgataccca agctttgcaa agaatggtaa tgtattccct attagcccct tgatagtttt
     1261 ctgaataggc acttctaaca attttgatta cttctttttc ttctaagggt tgatctaatc
     1321 gattattaaa ctcaaacata ttatattcgc acgtttcgat tgaatagcct gaactaaagt
     1381 aggctaaaga gagggtaaac atgacgttat tacgccctat taaacccttt tctcctgaaa
     1441 atttcgtttc gtgcaataag agattaaacc agggttcatc tacttgtttt ttgccttctg
     1501 taccgcttaa aaccgttaga cttgaacgag taaagccctt attatctgtt tgtttgaaag
     1561 accaatcttg ccattctttg aaagaataac ggtaattagg atcaaaaaat tctacattgt
     1621 ccgttcttgg tatgcgagca ataccaaaat gattacacgt tagatcaact ggcaaagact
     1681 ttccaaaata ttctcggata ttttgcgaaa ttattttggc tgctttgaca gatttaaatt
     1741 ctgattttga agtcacatag actggcgttt ctaaaacaaa atatgcttga taacctttat
     1801 cagatttgat aatcatagta ggcataaaac ctaaatcaat agcggttgtt aaaatatcgc
     1861 ttgctgaaat agtttctttt gccgtgtgaa tatcaaaatc aataaagaag gtattgattt
     1921 gtcttaaatt gttttcagaa tgtcctttcg tgtatgaacg gttttcgtct gcatacgttc
     1981 cataacgata aacgttgggt gtccaatgtg taaatgtatc ttgattttct tgaatcgctt
     2041 cctcggaagt cagaacaaca ccacgaccgc caatcatgct tgattttgag cgatacgcaa
     2101 aaatagcccc tttgctttta cctggcttgg tagtgattga gcgaatttta ctatttttaa
     2161 atttgtactt taacaagccg tcatgaagca cagtttctac aacaaaaggg atattcattc
     2221 agctgttctc ctttcctata aatcctataa aataggttgt ttaattaact tggtttgctt
     2281 tttcattcaa ctgtttcaat attgcatgtt ttgaaaaaga tttttttcct ttataagtca
     2341 atttttttcc actaatcgaa taaattattt tgttattttc tattaactta tatatataat
     2401 cttccccctc cgaagaaaaa tacttatctg attttgtttc taagtagata tttctctttt
     2461 ctaactcttt cttaaacgtt tctagtgtat agatatttgc taattttctt atctccaata
     2521 aactattttt tatataagtt ttacattcat catgattcat acaaactcca ccttctataa
     2581 atgaatacaa aaaaagcaat caaacgattt ccgattgatt gcttaacaat tcttaaattc
     2641 agtagcttag atacttgaaa actctctgat ttccctatat aatgatagta cggttatata
     2701 ccgtcttcaa acaaagttaa ttaaataact tcttacgagg gaagagttca tctgactaac
     2761 tgataagcgt tggtttggca atcttatcgg gctatgcatt tataaaatgt cgtcaaacat
     2821 tttataaatg tgtcatggct cttttttcgt ttctattcag ttcgttgttt cgttatatct
     2881 agtataccgc ttttaaaaaa aataagcaac gatttcgtgc attattcaca cgaagtcatt
     2941 gcttttttct tcttccattt ctaaatccaa tgttacttgt tctgattctg tttctggttc
     3001 tggttctgtt ggctcatttg ggattaaatc cactactagc gttgagttag ttaactttgc
     3061 aatttgttct agtgttttta tggttggatc tgattttcct gattctattc gtgaataatt
     3121 tgatctactc atttctaatt cttgggagaa tttttctttt gatttgccca aattccccgt
     3181 aggcgctagg gacctcttta gcttcttgga agctgtcagt agtatatcta ataatttatc
     3241 tccattccct ttagtaacgt gtaactttcc aaatttaaaa aagcgactca tagaattatt
     3301 tcctcccgtt aaataataga taactattaa aaatagacaa tacttgctca taagtaatgg
     3361 tacttaaatt gtttactttg gcgtgtttca ttgcttgatg aaactgattt ttagtaaaca
     3421 gttgacgata ttctcgattg acccattttg aaacaaagta cgtatatagc ttccaatatt
     3481 tatctggaac atctgtggta tggcgggtaa gttttattaa gacactgttt acttttggtt
     3541 taggatgaaa gcattccgct ggcagcttaa gcaattgctg aatcgagact tgagtgtgca
     3601 agagcaaccc tagtgttcgg tgaatatcca aggtacgctt gtagaatcct tcttcaacaa
     3661 tcagatagat gtcagacgca tggctttcaa aaaccacttt tttaataatt tgtgtgctta
     3721 aatggtaagg aatactccca acaattttat acctctgttt gttagggaat tgaaactgta
     3781 gaatatcttg gtgaattaaa gtgacacgaa tgttcagttt taatttttct gacgataagt
     3841 tgaatagatg actgtctaat tcaatagacg ttacctgttt acttatttta gccagtttcg
     3901 tcgttaaatg ccctttacct gttccaattt cgtaaacggt atcggtttct tttaaattca
     3961 attgttttat tatttggttg agtacttttt cactcgttaa aaagttttga gaatatttta
     4021 tatttttgtt catgtaatta ctcctgaagt gattacatct gtaaataaat acagaagtta
     4081 aacgatttgt ttgtaatttt agttatctgt ttaaaaagtc ataagattag tcactggtag
     4141 gaattaatct aacgtattta tttatctgcg taatcactgt ttttagtctg tttcaaaaca
     4201 gtagatgttt tatctacatt acgcatttgg aataccaaca tgacgaatcc ctccttctta
     4261 attacaaatt tttagcatct aatttaactt caattcctat tatacacaaa attttaagat
     4321 actgcactat caacacactc ttaagtttgc ttcgggggat cttttccgct gcataaccct
     4381 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     4441 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     4501 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     4561 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa
     4621 cagatgaggg caagcggatg gctgatgaaa ccaagccaac caggaagggc agcccaccta
     4681 tcaaggtgta ctgccttcca gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg
     4741 gcatgagcct gtcggcctac ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg
     4801 tggactatga gcacgtccgc gagctggccc gcatcaatgg cgacctgggc cgcctgggcg
     4861 gcctgctgaa actctggctc accgacgacc cgcgcacggc gcggttcggt gatgccacga
     4921 tcctcgccct gctggcgaag atcgaagaga agcaggacga gcttggcaag gtcatgatgg
     4981 gcgtggtccg cccgagggca gagccatgac ttttttagcc gctaaaacgg ccggggggtg
     5041 cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga agagcgactt cgcggagctg
     5101 gtgaagtaca tcaccgacga gcaaggcaag accgatcccc tatgcggtgt gaaataccgc
     5161 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     5221 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     5281 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     5341 cgacggccag tgccaagctt gcatgcctgc agttagttgg ccttctcggg caggtcgctg
     5401 taccgggcca cgctggcctc gtacagctcg aagtactcgt ccttcttctt ccgcagcatc
     5461 tggagccgga tgtcggtgaa gtggaagccg ggcatggtca gggcgcgcac ggccttcttg
     5521 ctccggtagc tggtgtagtg gtggcagatc aggtgccggt cgcccacctt cagggccatc
     5581 acgttccggc cgcacagcac gccgttctcg gggtacacca cctcggtgct gggctcccag
     5641 ccgccgctct tgttcttcat cacggggccg tcggcgggga agttggtgcc gtgcaccttc
     5701 accttgtaga tcaggcagtt gccctccagg ctggtgtcct ggtgggcggt caggatgccg
     5761 ccgtcctcgt aggtggtggt tctctcccag gtgaagccct cggggaagct ctgcttgaag
     5821 aagtcgggga tctcggcggt gtggtgcacg aaggtcctgc tgccgtactc gcagcagggg
     5881 gccaggatgt cgaaggcgaa gggcaggggg gcgccctcgg tcacgtggat tctcatgctc
     5941 tgggtgccgg cgaaggggtt gccgtcgccc tcgccctcgc acttgaagta gtggccgttc
     6001 acggtgccct ccatgtacat cttgatgcgc atactctcct tcagcaggcc gctcaccatt
     6061 ttaataaacc tcctttcgga tccgttgtaa atgaatttcc accttcaggg tttttgaaac
     6121 cataaaacca aagcaatcca agcaaaatag ctaggtagaa aaaagttttc attgtaaaaa
     6181 tagttgctgg atgatgcaaa attagtttta ttttattcat aaatccacct cttcattgtt
     6241 tcattagtgt tacaaatatt aaaaataaac gtttaatact tacttactgt aatacatttg
     6301 tctttaaatg tctaattaca tttttgtaag gttagaaaaa taaacatatt ttgaacttga
     6361 aagtaggtac gagtataatg aaacaataga tatagcaatg ttttaatgct agaaaagagg
     6421 ttggactatg attaatcaaa agaagattgc aggagaaaag gcttgtgaat ggattaaaga
     6481 tggaatggtt gtgggtcttg gaacgggggg taccgagctc gaattcgtaa tcatgtcata
     6541 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6601 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6661 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6721 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgag
//
