LOCUS       sequence-407120-       13580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NOT1 expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 13580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-mEGFP-NOT1 isoform C (HK53)
FEATURES             Location/Qualifiers
     source          1..13580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2517
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2537..2539,2540..2542,2543..3253)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2582..2603)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2843..2862)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3190..3211
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10838..10879
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10889..10906
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10929..10946)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10979..11113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11003..11022)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11057..11076
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11714..12302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11794..11813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12473..13264,13265..13333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13096..13115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13334..13438)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13506..13524
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaaaaatcat gaatggcatc aactctgaat caaatctttg cagatgcacc tacttctcat
       61 ttccactgtc acatcatttt tccagatctc gctgcctgtt atgtggccca caaaccaaga
      121 cacgttttat ggccattaaa gctggctgat cgtcgccaaa caccaaatac ataatgaata
      181 tgtacacatt cgagaaagaa gcgatcaaag aagcgtcttc gggcggagta ggagaatgcg
      241 gaggagaagg agaacgagct gatctagtat ctctccacaa tccaatgcca actgaccaac
      301 tggccatatt cggagcaatt tgaagccaat ttccatcgcc tggcgatcgc tccattcttg
      361 gctatatgtt tttcaccgtt acccggggcc attttcaaag actcgtcggc aagataagat
      421 tgtgtcactc gctgtctctc ttcatttgtc gaagaatgct gaggaatttc gcgatgacgt
      481 cggcgagtat tttgaagaat gagaataatt tgtatttata cgaaaatcag ttagtggaat
      541 tttctacaaa aacatgttat ctatagataa ttttgttgca aaatatgttg actatgacaa
      601 agattgtatg tatatacctt taatgtattc tcattttctt atgtatttat aatggcaatg
      661 atgatactga tgatatttta agatgatgcc agaccaaaag gcttgaattt ctgcgtcttt
      721 tgccgaacgc agtgcatgtg caattgttgt tttttggaat attcaatttt cggactgtcc
      781 gctttgattt cagtttcttg gcttattcaa aaagcaaagt aaagccaaaa aagcgagatg
      841 gcaataccaa atgcggcaaa acggtagtgg aaggaaaggg gtgcggggca gcggaaggaa
      901 gggtggggcg gggcgtggcg gggtctgtgg ctgggcgcga cgtcaccgac gttggagcca
      961 ctcctttgac catgtgtgcg tgtgtgtatt attcgtgtct cgccactcgc cggttgtttt
     1021 tttcttttta tgctgcgctc tctctagcgc catctcgctt acgcatgctc aacgcaccgc
     1081 atgttgccgt ttccttttat gcgtcatttt ggctcgaaat aggcaattat ttaaacaaag
     1141 attagtcaac gaaaacgcta aaataaataa gtctacaata tggttactta ttgccatgtg
     1201 tgtgcagcca acgatagcaa caaaagcaac aacacaggtg gctttccctc tttcactttt
     1261 tgtttgcaag ccgcgtgcga gcaagacggc acgaccggca aacgcaatta cgctgacaaa
     1321 gagcagacga agttttggcg aaaaacatca aggcgcctga tacgaatgca tttgcaataa
     1381 caattgcgat atttaatatt gtttatgaag ctgtttgact tcaaaacaca caaaaaaaaa
     1441 aataaaacaa attatttgaa agagaattag gaatcggacg cttatcgtta gggtaacaac
     1501 aagaaatgct tactgagtca cagcctctgg aaaactgccg caagccagag agagagagaa
     1561 aaagagggag agcagcttag accgcatgtg cttgtgtgtg aggcgtctct ctcttcgtct
     1621 ctgttgcgca aacgcataga ctgcactgaa aaaatcgatt acctattttt tatgaatgaa
     1681 tatttgcact attactattc aaaactatta agatagcaat cacattcaat agccaaatac
     1741 tataccacct gagcgatgca acgaaatgat caatttgagc aaaaatgctg catatttagg
     1801 acggcatcat tatagaaatg cttcttgctg tgtacttttc tctcgtctgg cagctgtttc
     1861 gccgttattg ttaaaaccgg cttaagttag gtgtgttttc tacgactagt gaatgcccta
     1921 ctagaagatg tgtgttgcac aaaatgtccc tggaataacc aatttgaagt gcagatagca
     1981 gtaaacgtaa gctaatatga atattattta actgtaatgt tttaatatcg ctggacatta
     2041 ctaataaacc cactataaac acatgtacat atgtatgttt tggcatacaa tgagtagttg
     2101 gggaaaaaat gtgtaaaagc accgtgacca tcacagcata aagataacca gctgaagtat
     2161 cgaatatgag taacccccaa attgaatcac atgccgcaac tgataggacc catggaagta
     2221 cactcttcat ggcgatatac aagacacaca caagcacgaa cacccagttg cggaggaaat
     2281 tctccgtaaa tgaaaaccca atcggcgaac aattcatacc catatatggt aaaagttttg
     2341 aacgcgactt gagagcggag agcattgcgg ctgataaggt tttagcgcta agcgggcttt
     2401 ataaaacggg ctgcgggacc agttttcata tcactaccgt ttgagttctt gtgctgtgtg
     2461 gatactcctc ccgacacaaa gccgctccat cagccagcag tcgtctaatc cagagacccc
     2521 ggatcggggt accatcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2581 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2641 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2701 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2761 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2821 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2881 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2941 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3001 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3061 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3121 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3181 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3241 cgagctgtac aagccaagct tgaattctgc agatatgaac gtagagagcc aactgaagac
     3301 accgccaacg cacattcgaa atctggtcaa gcacgtgaac aagcgaaatt ttaatgagag
     3361 cagcgagcaa ataaatcagt tcgttaaaga gcacggcctg gaagcggatc gcagcagcct
     3421 gcgccacctg ttctccgtga tcaacttcag cgatctcgtg ccatcagtta cggtccagct
     3481 gcaggcgaag ctcctgggca tccagttgga gcgtcagctt cacagttcat cgtttgtgtc
     3541 caacatctgc tacgcttttg accagttctt cgccagcaac cagaagtccc tgaagcctgt
     3601 tgcggtagcg gatctcgttg gccaggtcgc ccgactgacc ggcatcaaca aggtttgtga
     3661 gtgcgtcttt gccttggcct tgacccactc ctcgtacaca gaactcaaac attctgccag
     3721 gaacaaccta aaaggtagcc tcagcgaact tatagactct tacttgggta ataaaggcac
     3781 cggcccagcc gacagcggtc tccgggagat ctccttcgat ctactgcagt atttgctgtg
     3841 ctgccttagc gagtacgtac aaccgcaggt ggaagcacag tttttgatca agcttcgtga
     3901 ggagtttccg cgccaggcgg taccgctggt tctagcgccg ttcctttacg gctcgacgac
     3961 ggcgacgatt gcgggagcag gtgccagcga aacggatgcg gaggccgaag cgacgactaa
     4021 tagcaacagc tccagtttcg aagcagatgc tttgaacgag gtgggaatcg aggatattta
     4081 tgaccattta agcgagataa tattcaccaa ccagggcaaa aataatatta tggacacatc
     4141 ttggattaat ctaatccttg aaatcggtta tgaatttaca tcgagcgttg aagagtgtaa
     4201 gaatcatttg tgttcccgcg agcgagaacg cgctgagctc cagtccaaag atgtcgccaa
     4261 gatcgtgggt cttatgtgcc gacggcactc ctccctgctc gattgtaatg tgaatctacc
     4321 gacgccggcg aacttctggc ctggtcaggg acagggcggt ggcagcaaca gcagtggctc
     4381 ttcgcagaca caaatcactc cgcagcaaca gaacccgggc agcagtaaca acaacgatgg
     4441 cagcgatggc aattcgtcca gtgataagaa ggacaagaag gagacgacgg aggccacgca
     4501 aacttggaag ccagatgtct ttgtgcaggc gctcaaggag gtggtgcctc agctcaactg
     4561 gaaggacgtg tgcatggaac ttgatcatcc cgagttcgtg ctgaaggatc gcatcggttt
     4621 ggagcttttg ctcaccatcc ttcggctggc cactggctct aacatatttc cacatccaga
     4681 atgcatttac cgtcactggg caaacacgga aggtcagctg tcgcttatcg cgacaatgct
     4741 gaagaacccg gatctctttt ccttcgccga ctttgtattt agccagccag cgttggatgt
     4801 gcttaagacg gctccggatg cggacaataa ggagatctcg tcctggaagt cgcttcacct
     4861 ggtggaggtg ctgctctcca ttgcggacaa gggatactat acgcaggtcc atgagctatt
     4921 caagttccca gcgcagaact gtcccgatgt gctatttttg gccttattga acacaagtcc
     4981 gcctatgacg ccactgcgtc aggatctgtt taaccaactg ataccaacgt tccttggcaa
     5041 ccatcccaac tcgaacgtga tcttggccag cgcctggagc tcgaacaact tccaacttcg
     5101 ttccaatatc atgaatgcta tgtccgagtg gtatctccga ggcaatgagt tcgatcaggt
     5161 gaaactgtcg cgcatcctgg atcttgccca ggatttaaaa gcgctatcag cgctgctcaa
     5221 cgcccgttca tttttattca ttatcgacct ggcttgcctt gcctctcggc gcgagtacct
     5281 gaagctggaa aagtggctta ccgataaaat tcgcgaacat ggagagcctt ttatgcaggc
     5341 tataatcaag gttctgcacc gccgctgccc gcaggttatc aacgccaaag tgccagagga
     5401 tcagctgcct cccaagcagg cgcagctttt gcccgagaca gttacaacca tgatcaactg
     5461 tctgcaaacg tgcatcaata actgcatgca gccggagatg gtcgaagtca tcatgcaaat
     5521 gaccgcgaac gtggcgatta tggctaacaa ggcgcgtgcc caacagcagc agcagccagg
     5581 attggttccg ccgcctccac caacaattct gcgcggccat cgtggtatgg atctgcctgg
     5641 aggcatagtc cctccgccac cgcagcaacc gttctccgga aacctcaacg cgcagatgtt
     5701 tggaccagga atggatccgc taacgaatat gtccaacaac ttagccggcc tcaacctcag
     5761 cggcccgaac ggagcattta attttggaaa catgctaact tctccatcgc gtctgatgac
     5821 tccgggagcc aatccctatc cgctgaacct catgcagatg ccgcaggctc cgccaccacc
     5881 caacgtagga aacctaggcc gcatgctgcc agggggtcca caacagcaga ctccaacgcc
     5941 gactccaacg gcccccaatc caaacaaccc tgtgatggcc gatctccaga tacctgtgtc
     6001 aaaggaagtt gaagacgagg tcaactcata ctttcagcgc atctacaacc accagccgaa
     6061 tccaacgctg tccatcgatg aagtgctgga cattttgcaa agatttaaag agtcaagtaa
     6121 ccggcgcgag caggaagtct ttttgtgcat gctgcgcaat ctgttcgagg aatatcgctt
     6181 tttctgtcaa tacccagaga aagagctaca gattactgcg cagctatttg gtggcattat
     6241 tgatcgtaac ctggtgccca cttttgttgc actgggcctt tctctgcgct gtgtcttgga
     6301 tgccctccgc aagcccgatg gatctaagct ttactatttc ggtgtgacgg cactggacag
     6361 attcagaact cgattgcaca cctacaacaa atactgcgag catatccgct ccattcctca
     6421 cttctctgac ttcccgccgc accttattca gtatgtggaa tacggaatgc acggccagga
     6481 gccgccgccg cagaagttga ttggactcag caatacgatt ccttcggcga tatctagcgg
     6541 accaggaacg gaaccaattt acaggaatag ctccatgtta ggtaatatgc ccgctgccac
     6601 cccgggatcg ggaccgaaat caaacgctgc tgtatcgcat gccacgagaa tgaagtccat
     6661 cgccaatgcc accaatatcg acacactttt ggtggccaac caggaagaga aagtgactgt
     6721 gccaccagag cctgtgcagg acaaaactgc ctttattttc aacaacttga gccagctgaa
     6781 cataccgcag aagtgcgatg agataaagga gattatgacc aaggagtatt ggccgtggct
     6841 agcgcaatat ctggtcctca aacgcgcatc aatggagttc aacttccaca cactatatta
     6901 caactttttg gatgccctta aaaacggcga gatcaaccga ttcgtaacca aagagaccct
     6961 acgcaacatc aaggtactct tgcgctccga taagggagtt attaatttct ccgatcgaag
     7021 tctgttgaag aacctcggac actggttggg catgatgacc ttaggtcgca atcgccccat
     7081 cctgcagttg gatctggatc ttaaatccct tttggctgag gcttaccaca agggccagca
     7141 ggaactgctt tttgtggttc ctttcgtagc caagatcctt gagtcatccg ccaagtctcg
     7201 catcttccga tcgcccaatc catggacaat gggtatcatg tacgtgcttg ctgagcttca
     7261 ccaggagcca gaccttaagc tgaacctgaa gtttgaaatc gaagtactct gcaaaacact
     7321 taatctggag ttggccaagt tgcgacaagt gatctacctg aaggatccca atcgaactca
     7381 ccggattgaa gagcagatgt cgcaaccaaa accgaaacaa cttgagccag tggcttctgc
     7441 gccagctcta ccgcgtgaac agcaatcccc ggcacaacct ccgccaccac cacaacagca
     7501 gcaaccaccg cagcagcagg tacctccacc gccatcctct gcggacgtgg acgcccaaaa
     7561 tgcggccgct atgatgatgg cagcgggcgg agctaatagt actcctggat cggtatcttc
     7621 gcccaatcta cccaccgatt ccagccaggt ggcactaccg ccaccagagc cgcgctattc
     7681 atacgtggac gtaaacgtga gcaacttcca gcttattggt cagcagttgg tactgccacc
     7741 caatactccg ttcctgcacg ccaatcccgg aatcaaacac atggtcgtca acgccgtgga
     7801 gcgcacgata accgactggt tgcagcctat tgtggaccga agcatccgca ttgcctgtgc
     7861 caccaccgag cagatcatcc gcaaggactt cgccctggat gctgacgaga accgaatgcg
     7921 cactgccgct catcagatgg tacgaaacct tgccgccggc atggccatga ttaccggtaa
     7981 ggatgagata gcgcgtgcta ttagccagaa tctgcacaag gccttgctgt cgggtctaaa
     8041 tgggatgccc agcatggctg agatccaagc tgctgctatg cagttagcca gcgaaaatgt
     8101 tgagctagtt tgcgctttca ttcagaaaac atctgctgaa aaggcagccg cagagatcga
     8161 taggcgtcta agcaccgatt tcgagaccag aaaaattgct cgtgaagagg gcaaccgctt
     8221 tgtggacgcc cagatcctca cctatcaaca ggagcgtctt ccagaagcag tgcgcatcaa
     8281 ggtgggcgcg gctccagcta cactttatgc tgtgtactcg gagttcgcta gaagcatacc
     8341 tggcttccag cagatgagcg atcgcgatat tgccctgttt gtgcccaagc cgacggattt
     8401 gtctcagcca aatgtgtttg ctaatgatga tagcatggta tacggggaac tggctagcaa
     8461 aatggaggcc ttcatgaaca cggcaatcgg agtgccgaca ctccagatcc aggctagcaa
     8521 gatgcacatg ctactcaacg ccctgatagc gacgcgtcga ctgcgtgacc aagagtctgc
     8581 ctttaatctg ctgacccgcg cggtcgaggg tttgaccgaa ggattggtca atatgcatga
     8641 aaacatggag caaatgaaga tgtaccaaaa catccatctc cgtatccttg gcttgctgaa
     8701 caacagtttc ggtgctccaa acacagagcg agcggtaaca aagtgtttct tcgatatccg
     8761 agaggaggtg cgatataacg tggaggcagc tcgcgctttg attacgtcgc actttgtcaa
     8821 tctgaatcag tttgacggaa tgctacgcga ctgcatggac aacggaaaca attatgtggc
     8881 tatatcgttc ggtatcgcac tgctggagcg cctcatcatg gacgatcgag tgattaatat
     8941 tgtttcggac aacgaattca tggcaactgt tgagctgcta ggtaggctca ctcagcatcg
     9001 ccatcggtat ccagaatgca ttgtgaatgc tattgatacg ctctggagcg gaaactttaa
     9061 ctcgagcagc gactacagcc cgttcaacgg caatgatcgt tacttgtcgg gagcctccca
     9121 ttacatccac tccggcatgc atcacgtaag gtcatgcgat acagatgatc cgccgggctt
     9181 acaagagaag acggagtttc tgcttaagga ttgggttgca ctgtacacgc aacagaacca
     9241 gcagtcaacg cgcgatgcac gcaacttcag tgcatttgtt cagaagatga acacatatgg
     9301 tatcctgaaa acggacgact tgatcacccg ttttttccgc caggccacac acatttgcac
     9361 ggatgtggtg tacagaatgt tcgccgagcc gagtttacca atcaaccagg ccaaaaacaa
     9421 gatattccag tggatcgatg cgttcgtcca tctgattgca atgctcgtgc gacactcagg
     9481 cgaggcaggt aatccaacca ccaagatcaa cctgttaaac aaggtgctgg gcattgtgct
     9541 gggaacgctg attaaggacc atgagatgcg tggcgttagc ttccagcagg ttggctacca
     9601 tcgcttcttc atgatgctgt tcatggagct gtgcacggcc gatgtgattc tcgagtccct
     9661 gatgcacagt attgtgtcgg ccttcgccta cacatatcac ttgttgaatc ccagcgtggc
     9721 tccgggattc tgctttgctt ggttggagct catttcgcat cgcgtcttcc tgggccgcat
     9781 cctggtccaa attcccgggc aaaagggctg gccactttac gctcaattgc tgcaggacct
     9841 ttttaaatat cttgcgccct tcttacgcaa cactgagctc ggcaaaccag tccagctttt
     9901 gtacaagggg atgctacgtg ttctgctcgt tctgcttcac gacttcccgg agttcttgtg
     9961 cgactatcat tttgggttct gtgacaccat cccgcccaat tgcgtccaga tgcgtaacat
    10021 catcctgtcg gcgtttccgc gcaacatgcg cttgcccgat cccttcacgc ccaacctaaa
    10081 agtggacatg ctgtcggaca gtagtaatgc gcctaaggtg ctcagtagct acattatgaa
    10141 catccagccc gcgaacttta agaaggattt ggactcgtac ctcaaggccc gggcgccagt
    10201 tacttttcta tcggaattac gtggtcactt gcaggtgaca agcgagccag gaactcgtta
    10261 caacatggcg ctgatgaatg ctctggtcat gtatgtggga actcaggcga ttgctttgat
    10321 cagaaacaaa aactttgtgc ccaacacctc gaacatagca cacagcgccc acatggatat
    10381 cttccagaat cttgctgttg acttggatac ggagggtcgc tatctgttct tgaatgcgat
    10441 tgcgaaccag ttgcgctatc ccaacagcca cacgcactac ttcagctgtg cggtgctgca
    10501 tctgtttgcc gaggccaact cggaggcaat tcaggagcag atcactcgcg tacttctgga
    10561 acgattgatc gtgaaccgcc cgcatccttg gggactgttg atcactttta ttgagctgat
    10621 caaaaatccc atctacaagt tctgggacca tgactttgtg cactgtgcgc cagagatcac
    10681 caagctcttt gaatcggtgg cccgttcctg tctggccaag tcgaacgtca cccagcagtt
    10741 gaacatgccc gtcgtcgatg gcgagggcca ggaggtagcc accatcaaca tccagcacag
    10801 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
    10861 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
    10921 ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga
    10981 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
    11041 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
    11101 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
    11161 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
    11221 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
    11281 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
    11341 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
    11401 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
    11461 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
    11521 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
    11581 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
    11641 atgacgacca tcagggacag cttcaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    12001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    12061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    12121 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    12181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    12241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    12301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    12361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    12421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    12481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    12541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    12601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    12661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12781 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    13021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    13081 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    13141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    13201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    13261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    13321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    13381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    13441 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    13501 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attaattcta tattctaaaa
    13561 acacaaatga tacttctaaa
//
