LOCUS       sequence-407124-        9429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-Tagged human HNRNPQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9429)
  TITLE     Alarcon lab plasmids
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label:  pLenti-C-Myc-DDK-IRES-Puro HNRNPQ
FEATURES             Location/Qualifiers
     source          1..9429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(666..1325)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1240..1259
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1326..1430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1505..1524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1695..2074
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2076..2274
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2292..2472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2519..2644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3141..3374
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3559..3603
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3901..4018
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4101..4480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4481..4684
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4710..4729
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4710..4728
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6673..6702
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6721..6744
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6778..7351
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6801..7353
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6945..6962)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7172..7191
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7353..7952
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7353..7372)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7849..7869
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7982..8015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8071..8659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8124..8144)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8542..8553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8662..8678)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8663..8679)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9188..9368
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      721 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      781 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      841 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
      901 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      961 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1021 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1081 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
     1141 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1201 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1261 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1321 tccatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1441 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     1501 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     1561 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     1621 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     1681 ccagatatcg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1741 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1801 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1861 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1921 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1981 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2041 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2101 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2161 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2221 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     2281 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2341 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2401 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2461 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     2521 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     2581 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     2641 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     2701 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     2761 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     2821 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     2881 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     2941 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     3001 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3061 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3121 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3181 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3241 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3301 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3361 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3421 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3481 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3541 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3601 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3661 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3721 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     3781 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     3841 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     3901 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     3961 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4021 ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc tctagacatg
     4081 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     4141 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4201 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4261 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4321 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     4381 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     4441 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4501 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4561 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     4621 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4681 agctcgttta gtgaaccgtc agaattttgt aatacgactc actatagggc ggccgggaat
     4741 tcgtcgactg gatccggtac cgaggagatc tgccgccgcg atcgcatggc tacagaacat
     4801 gttaatggaa atggtactga agagcccatg gatactactt ctgcagttat ccattcagaa
     4861 aattttcaga cattgcttga tgctggttta ccacagaaag ttgctgaaaa actagatgaa
     4921 atttacgttg cagggctagt tgcacatagt gatttagatg aaagagctat tgaagcttta
     4981 aaagaattca atgaagacgg tgcattggca gttcttcaac agtttaaaga cagtgatctc
     5041 tctcatgttc agaacaaaag tgccttttta tgtggagtca tgaagactta caggcagaga
     5101 gaaaaacaag ggaccaaagt agcagattct agtaaaggac cagatgaggc aaaaattaag
     5161 gcactcttgg aaagaacagg ctacacactt gatgtgacca ctggacagag gaagtatgga
     5221 ggaccacctc cagattccgt ttattcaggt cagcagcctt ctgttggcac tgagatattt
     5281 gtgggaaaga tcccaagaga tctatttgag gatgaacttg ttccattatt tgagaaagct
     5341 ggacctatat gggatcttcg tctaatgatg gatccactca ctggtctcaa tagaggttat
     5401 gcgtttgtca ctttttgtac aaaagaagca gctcaggagg ctgttaaact gtataataat
     5461 catgaaattc gttctggaaa acatattggt gtctgcatct cagttgccaa caataggctt
     5521 tttgtgggct ctattcctaa gagtaaaacc aaggaacaga ttcttgaaga atttagcaaa
     5581 gtaacagagg gtcttacaga cgtcatttta taccaccaac cggatgacaa gaaaaaaaac
     5641 agaggctttt gctttcttga atatgaagat cacaaaacag ctgcccaggc aaggcgtagg
     5701 ttaatgagtg gtaaagtcaa ggtctggggg aatgttggaa ctgttgaatg ggctgatcct
     5761 atagaagatc ctgatcctga ggttatggca aaggtaaaag tgctgtttgt acgcaacctt
     5821 gccaatactg taacagaaga gattttagaa aaggcattta gtcagtttgg gaaactggaa
     5881 cgagtgaaga agttaaaaga ttatgcgttc attcattttg atgagcgaga tggtgctgtc
     5941 aaggctatgg aagaaatgaa tggcaaagac ttggagggag aaaatattga aattgttttt
     6001 gccaagccac cagatcagaa aaggaaagaa agaaaagctc agaggcaagc agcaaaaaat
     6061 caaatgtatg acgattacta ctattatggt ccacctcata tgccccctcc aacaagaggt
     6121 cgagggcgtg gaggtagagg tggttatgga tatcctccag attattatgg atatgaagat
     6181 tattatgatt attatggtta tgattaccat aactatcgtg gtggatatga agatccatac
     6241 tatggttatg aagattttca agttggagct agaggaaggg gtggtagagg agcaaggggt
     6301 gctgctccat ccagaggtcg tggggctgct cctccccgcg gtagagccgg ttattcacag
     6361 agaggaggtc ctggatcagc aagaggcgtt cgaggtgcga gaggaggtgc ccaacaacaa
     6421 agaggccgcg gggtacgtgg tgcgaggggt ggccgcggtg gaaatgtagg aggaaagcgc
     6481 aaagctgatg ggtacaacca gccagattcc aagcggcgcc agaccaataa tcagaactgg
     6541 ggctcccaac ccattgctca gcaaccgctc caaggtggtg atcattctgg taactatggt
     6601 tacaaatctg aaaaccagga gttttatcag gatacttttg ggcaacagtg gaagacgcgt
     6661 acgcggccgc tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg
     6721 gattacaagg atgacgacga taaggtttaa acctaggcgt agcggccgca aattccgccc
     6781 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6841 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6901 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6961 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     7021 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     7081 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     7141 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     7201 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     7261 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     7321 cgtggttttc ctttgaaaaa cacgatgata atatgaccga gtacaagccc acggtgcgcc
     7381 tcgccacccg cgacgacgtc ccccgggcag tacgcaccct cgccgccgcg ttcgccgact
     7441 accccgccac gcgccacacc gtcgatccag accgccacat cgagcgggtc accgagctgc
     7501 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     7561 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     7621 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     7681 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     7741 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     7801 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7861 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7921 cctggtgcat gacccgcaag cccggtgcct gatgtacaag taggattcgt cgagggacct
     7981 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
     8041 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     8101 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8161 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8221 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8281 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8341 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8401 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8461 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8521 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8581 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8641 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     8701 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     8761 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     8821 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8881 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     8941 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     9001 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     9061 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     9121 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     9181 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9241 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9301 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9361 ctctagcagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9421 gctggcgtt
//
