LOCUS       sequence-407153-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-EGR1-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(128..196,197..988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(346..365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1159..1747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1648..1667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1901..1918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2035..2056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2071..2076,2077..2094,2095..2101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2109..2125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2114..2136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2133..2149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2133..2149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2198..2319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2209..2228)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2263..2282
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2449..2823)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2842..2889)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2937..3266)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2951..3086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3005..3024)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3251..3271
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3280..3708)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3390..3411)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3602..3621
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3754..3978
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3967..3984
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4010..4190
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4741..4757
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4742..4758
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4761..5349
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4867..4878
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5276..5296
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5372..6079)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5650..5669)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(6089..6145)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      7795..7804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7884..7908)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7905..7943)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(7976..7994,7995..8017,8018..8036,8037..8059,8060..8078,
                     8079..8101,8102..8120,8121..8143,8144..8162,8163..8185,
                     8186..8204,8205..8227,8228..8246)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    8303..8420
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8718..8762)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8947..9180
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9673..9798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9845..10025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10043..10241)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        10243..10622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10794..10813
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct ggtaaaagta aaagatgctg aagatcagtt
      241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1861 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1921 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2041 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     2101 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     2161 caagctctag ctagaggtcg acggtataca gacatgataa gatacattga tgagtttgga
     2221 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2281 gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag
     2341 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     2401 caacctttca tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc
     2461 ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca
     2521 cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac
     2581 gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt
     2641 gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac
     2701 cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa
     2761 ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc
     2821 catggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta
     2881 attgtcaaca cgtgctgatc agatccgaaa atggatatac aagctcccgg gagctttttg
     2941 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag
     3001 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     3061 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     3121 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     3181 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     3241 gactttccac accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt
     3301 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     3361 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3421 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3481 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3541 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3601 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3661 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc
     3721 tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
     3781 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     3841 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     3901 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     3961 ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct gctagagatt
     4021 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     4081 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     4141 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc
     4201 ggatgcagct ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat
     4261 acttctctct ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc
     4321 tctccttcat tggcttcttc taccttctct tgctcaactg gtactagttc tgctaggtca
     4381 cccttttcaa tctacggaga tgaccagact agccttgtag caccatccaa aggtcagtgg
     4441 atatctgatc cctggccctg gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg
     4501 gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc cttccagtcc
     4561 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     4621 aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc
     4681 acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt tttctaggtc
     4741 tcgaggtcga cggtatcgat gcggggaagc ggcccaaagg gagatccgac tcgtctgagg
     4801 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     4861 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     4921 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     4981 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     5041 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     5101 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     5161 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     5221 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     5281 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     5341 aggttgattg tcgacttaac gcgttctaga ttcacttgta cagctcgtcc atgccgccgg
     5401 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
     5461 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
     5521 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
     5581 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     5641 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
     5701 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
     5761 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
     5821 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
     5881 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     5941 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     6001 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
     6061 cctcctcgcc cttgctcact ccggagccag ggccagggtt ctcctcgacg tcgccagcct
     6121 gcttcagcag ggaaaagttg gtggccccgg atccatcgaa ttcacgcgtg caaatttcaa
     6181 ttgtcctggg agaaaaggtt gctgtcatgt ccgaaagccc tgtggaggcg ctgaaggagt
     6241 tggtgacagc tgaggaaggg aagctgctga cctgggccgg gaaagcaggg ggaacagagg
     6301 agtacgtggt ggccaccgac ggggagggga agccactgtg cacaggggat gggtaggtcg
     6361 aggagccggg agaggagaag gaggtgggca caggggatgg gtatgaggtg gtggccgggg
     6421 atggataaga ggtagtaacc ggggacgggt aagaggtagc aaccggggac gggtaggaag
     6481 agagagagga ggtggccgaa gaggccacaa cacttttgtc tgctttcttg tccttctgcc
     6541 gcaagtggat cttggtatgc ctcttgcgtt catcgctcct ggcaaacttt cttccacaga
     6601 tgtcgcaggc gaagggcttt tcgcctgtgt gggtgcggat gtgggtggtg aggtggtcgc
     6661 tgcggctgaa gttgcgcatg cagatgcggc actggaaggg cttctggcct gtgtggatgc
     6721 ggatgtggcg ggtgagctcg tcggagcggg agaagcggcg atcacaggac tccactgggc
     6781 aagcgtaagg gcgttcgtgg gggggcgtct tgctgggccg gttggggtac ttgcgcatgc
     6841 ggctgggttt gatgagctgg gactggtagc tggtattgag ggccttcagg tcctgggagc
     6901 ccgactgagt ggcaaaggcc ttaatagtag acagaggggt tagcgaaggc tgctgggtgc
     6961 ggctctccag gccctggaag ggcttctggt ctggggtgcc caggcccaga tccccctgct
     7021 gctgtggaaa caggtagtcg gggatcatgg gaacctggaa gccacccttg gcggcagggt
     7081 aggcaggagg cgggtactgg agcgctgtcc ctgccgagcc cgggaaggcc tggctttgtg
     7141 gctcagggaa aatgtcagtg ttcggcgtgg ggaaggtggg tgccgctgag taaatgggac
     7201 tgctgtcgtt ggatggcact gcgcagctca ggggtgggct ctgggaggcg gaggcggagg
     7261 aggccgctgg agatggtgct gaggacgagg aggccggtgg gttggtcatg ctcactaggc
     7321 cactgaccaa gctgaagagg ggctcgggcc acaaggtgtt gccactgttg ggtgcaggct
     7381 ccagggaaaa gcggccagta taggtgatgg ggggcagtcg agtggtttgg ctggggtaac
     7441 tggtctccac cagcaccttc tcgttgttca gagagatgtc aggaaaagac tctgcggtca
     7501 ggtgctcgta gggctgctcg cccgtgtccg cctgagggtt gaaggtgctg ctgctgctgc
     7561 tgctgttgct gccgcccccg ccgcctccac cgcccccgct gctgctgctg ctgctgttgc
     7621 tgccgctgcc ctctggggcc ccggcggcgc cgaggaactg gggagccccg ttgctcagca
     7681 gcatcatctc ctccagctta gggtagttgt ccatggtggg cgagtgagga aaggatccga
     7741 acgggtcaga gatctgcagc ggggacatca gctgcatctc ggccttggcc gcggccatgg
     7801 tggcggaatt cggggccgcg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa
     7861 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
     7921 gcctcccacc gtacacgcct acctcgaccc gggtaccgag ctcgacttcc acttttctct
     7981 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     8041 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     8101 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     8161 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     8221 tcacttttct ctatcactga tagggagtgg taaactcgag atctaaccaa actggatctc
     8281 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     8341 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     8401 tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtctgcag aattggcgca
     8461 cgcagtgccg atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     8521 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     8581 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     8641 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     8701 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     8761 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     8821 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     8881 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     8941 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     9001 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     9061 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     9121 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     9181 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     9241 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     9301 atatctcctc ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt
     9361 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     9421 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     9481 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     9541 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     9601 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     9661 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     9721 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     9781 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     9841 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     9901 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     9961 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
    10021 gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac gcctaccgcc
    10081 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
    10141 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
    10201 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
    10261 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
    10321 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
    10381 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
    10441 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
    10501 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
    10561 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
    10621 tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
    10681 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
    10741 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
    10801 agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg
    10861 tcagg
//
