LOCUS       sequence-407156-       13244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 13244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-KDM2B-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..13244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2613..2631,2632..2654,2655..2673,2674..2696,2697..2715,
                     2716..2738,2739..2757,2758..2780,2781..2799,2800..2822,
                     2823..2841,2842..2864,2865..2883)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2916..2954
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2951..2975
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3055..3064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7093..7149
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7159..7866
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7301..7319)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7569..7588
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7889..8477
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7942..7962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8360..8371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8480..8496)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8481..8497)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9048..9228
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9254..9271)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9260..9484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9530..9958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9617..9636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9827..9848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9967..9987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9972..10301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10152..10287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10214..10233
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10349..10396
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10415..10789
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10919..11040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10956..10975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11010..11029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11089..11105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11089..11105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11102..11124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11113..11129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11137..11143,11144..11161,11162..11167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11182..11203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11320..11337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11491..12079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11571..11590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12250..13041,13042..13110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12873..12892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13111..13215)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2881 agaaaagtgg aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc
     3061 atggcgggtc cgcaaatggg gggatctgca gaggatcacc ccccacgaaa aagacatgca
     3121 gcagaaaagc aaaaaaagaa aacagttata tatacaaaat gctttgaatt tgagtcggcc
     3181 acacagcgcc cgattgaccg ccagcgatac gacgagaacg aggacttgtc ggacgtggag
     3241 gagatcgtca gcgtccgcgg cttcagcctg gaggagaagc ttcgcagcca gctgtaccag
     3301 ggggacttcg tgcacgccat ggagggcaaa gatttcaact atgagtacgt acagagagaa
     3361 gctctcaggg ttcccctgat atttcgagaa aaggatggac tgggaattaa gatgcctgac
     3421 cctgatttca cagtccgaga cgtcaaactc ctagtgggga gccggcggct tgtggacgtg
     3481 atggatgtga acacccagaa gggcacggag atgagcatgt cccagtttgt gcgttactac
     3541 gagacgcccg aggcccagcg ggacaagctg tacaacgtca tcagcctaga gttcagccac
     3601 accaagctgg agcacttggt caagcgtccg actgtggtag acctggtgga ctgggtggac
     3661 aacatgtggc cccagcatct gaaggagaag cagacagaag ccacgaacgc cattgcagag
     3721 atgaagtacc cgaaagtgaa aaagtactgt ctgatgagcg tgaaaggttg tttcaccgac
     3781 ttccacatcg actttggagg cacttccgtt tggtaccatg ttttccgggg tgggaagatt
     3841 ttttggctga ttcctccaac gctgcacaat ttggcgctgt acgaggagtg ggtgctgtca
     3901 ggcaaacaga gtgacatctt tctgggagac cgtgtggaac gatgccaaag aattgagctg
     3961 aagcagggct acacattttt catcccttcc ggttggatcc atgccgtcta cacccctgta
     4021 gactctttgg tgttcggcgg aaacatcctg cacagcttta acgtgcccat gcagctgcgg
     4081 atctacgaga tcgaggacag gacgcgggtg cagcccaaat tccgttaccc cttctactat
     4141 gagatgtgct ggtatgtcct ggagagatac gtgtactgtg tgacccagcg ctcccacctc
     4201 actcaggaat accagaggga gtcgatgctt attgatgccc cgaggaagcc cagcatagac
     4261 ggcttctctt cggattcctg gctggagatg gaggaggagg cctgtgatca gcagcctcag
     4321 gaggaggagg agaaggacga ggagggcgag ggcagggaca gggcacccaa accgcccacc
     4381 gatggctcca cttcacccac cagcacgccc tctgaggacc aggaggccct cgggaagaag
     4441 cccaaagcac ctgccctgcg attcctcaaa aggactttgt ctaatgagtc ggaggaaagt
     4501 gtgaagtcca ccacattggc cgtagactac cccaagaccc ccaccggctc tcccgccacg
     4561 gaggtctctg ccaaatggac ccatctcact gagtttgaac tgaagggcct gaaagctctg
     4621 gtggagaaac tggaatccct cccggagaac aagaagtgtg tccccgaggg catcgaggac
     4681 ccccaggcac tcctggaggg tgtgaagaac gtcctgaagg agcacgcaga tgatgaccct
     4741 agtctggcca tcactggggt ccctgtggtg acttggccaa agaagactcc aaagaaccgg
     4801 gctgtgggtc ggcccaaggg gaagctgggc ccggcctccg cggtgaagtt ggccgccaac
     4861 cggacaacgg caggagctcg gcggcgccgg acgcgatgcc gcaagtgcga ggcctgcctg
     4921 cggaccgagt gcggagagtg ccacttctgc aaggacatga agaagttcgg gggccccggg
     4981 cgcatgaagc agagctgcat catgcggcag tgcatcgcgc cagtgctgcc ccacaccgcc
     5041 gtgtgccttg tgtgtggcga ggcggggaag gaagacacgg tggaagagga ggaaggcaag
     5101 tttaacctca tgctcatgga gtgctccatc tgcaatgaaa tcatccaccc tggatgcctt
     5161 aagattaagg agtcagaggg tgtggtcaac gacgagcttc caaactgctg ggagtgtccg
     5221 aagtgtaacc acgccggcaa gaccgggaaa caaaagcgtg gccctggctt taagtacgcc
     5281 tccaacctgc ccggctccct gctcaaggag cagaagatga accgggacaa caaggaaggg
     5341 caggaacctg ccaagcggag gagtgagtgt gaggaggcgc cccggcgcag gtcggatgag
     5401 cactcgaaga aggtgccgcc ggacggcctt ctgcgcagaa agtctgacga cgtgcacctg
     5461 aggaagaagc ggaaatacga gaagccccag gagctgagtg gacgcaagcg ggcctcatcg
     5521 cttcaaacgt cccccggttc ctcctctcac ctctcgccga ggccccctct aggcagcagc
     5581 ctcagcccct ggtggagatc cagtctcact tacttccagc agcagctcaa acctggcaaa
     5641 gaagataagc ttttcaggaa aaagcggcgg tcctggaaga acgccgagga ccgcatggcg
     5701 ctggccaaca agcccctccg gcgcttcaag caggaacccg aggacgaact gcccgaggcg
     5761 ccccccaaga ccagggagag cgaccactcc cgctccagct cccccaccgc gggacccagc
     5821 accgaagggg ccgagggccc ggaggagaag aagaaggtga agatgcgccg gaagcggcgg
     5881 cttcccaaca aggagctgag cagggagctg agcaaggagc tcaaccacga gatccagagg
     5941 acggagaaca gcctggccaa cgagaaccag cagcccatca agtcggagcc tgagagcgag
     6001 ggcgaggagc ccaagcggcc cccgggcatc tgcgagcgtc cccaccgctt cagcaagggg
     6061 ctcaacggca ccccccggga gctgcggcac cagctggggc ccagcctgcg cagcccgccc
     6121 cgtgtcatct cccggccccc accctccgtg tccccgccca agtgtatcca gatggagcgc
     6181 catgtgatcc ggccaccccc catcagcccc ccgcctgact cgctacccct ggacgatggg
     6241 gcagcccacg tcatgcacag ggaggtgtgg atggccgtct tcagctacct cagccaccaa
     6301 gacctgtgtg tgtgcatgcg ggtctgcagg acctggaacc gctggtgctg cgataagcgg
     6361 ttgtggaccc gcattgacct gaaccactgc aagtctatca cacccctgat gctgagtggc
     6421 atcatccggc gacagcccgt ctccctcgac ctcagctgga ccaatatctc caagaagcag
     6481 ctgagctggc tcatcaaccg gctgcctggg ctccgggact tggtgctgtc aggctgctca
     6541 tggatcgcgg tctcggccct ttgcagctcc agttgtccgc tgctccggac cctggatgtc
     6601 cagtgggtgg agggactaaa ggatgcccag atgcgggatc tcctgtcccc gcccacagac
     6661 aacaggccag gtcagatgga caatcggagc aagctccgga acatcgtgga gctgcgcctg
     6721 gcaggcctgg acatcacaga tgcctccctg cggctcatca tccgccacat gcccctgctc
     6781 tccaagctcc acctcagtta ctgtaaccac gtcaccgacc agtctatcaa cctgctcact
     6841 gctgttggca ccaccacccg agactcctta accgagatca acctgtctga ctgcaataag
     6901 gtcactgatc agtgcctgtc cttcttcaaa cgctgtggaa acatctgtca tattgacctg
     6961 aggtactgca agcaagtcac caaggaaggc tgtgagcagt tcatagccga gatgtctgtg
     7021 agtgtccagt ttgggcaagt agaagaaaaa ctcctgcaaa aactgagtac gcgtgaattc
     7081 gatggatccg gggccaccaa cttttccctg ctgaagcagg ctggcgacgt cgaggagaac
     7141 cctggccctg gctccggagt gagcaagggc gaggaggata acatggccat catcaaggag
     7201 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     7261 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     7321 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     7381 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     7441 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     7501 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     7561 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     7621 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     7681 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     7741 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     7801 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     7861 aagtgaatct agaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8281 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8461 ttgggccgct tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     8521 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     8581 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     8641 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8701 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     8761 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     8821 caaggctagg wtsksgsmgt acctttgatg tcctgattgg actagcagaa ctagtaccag
     8881 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     8941 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     9001 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     9061 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9121 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9181 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     9241 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     9301 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     9361 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     9421 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     9481 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     9541 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9601 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9661 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9721 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9781 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9841 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9901 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     9961 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    10021 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
    10081 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
    10141 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
    10201 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
    10261 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
    10321 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
    10381 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
    10441 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
    10501 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
    10561 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
    10621 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
    10681 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
    10741 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
    10801 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    10861 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    10921 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10981 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11041 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    11101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    11161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    11221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    11341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    11401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    11461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    12001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    12061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    12121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    12181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    12241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    12301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    12361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    12421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    12481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    12541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    12601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    13021 ttttaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    13081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    13141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    13201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacg
//
