LOCUS       sequence-407163-        6359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAF1 expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-lambda-N-HA-CAF1 isoform A (HK101)
FEATURES             Location/Qualifiers
     source          1..6359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..81
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1096..1137
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1147..1164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1187..1204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1237..1371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1261..1280)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1315..1334
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(1972..2560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2052..2071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2731..3522,3523..3591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3354..3373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3592..3696)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3764..3782
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3846..6355
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 gatcggggta ccaacatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa
       61 gctcaatgga aagctgcaaa cggtggatct cctccacata tggcttaccc atacgatgtt
      121 ccagattacg ctcctccaag cttgaattct gcagatatga aatggacaat gccctcggcc
      181 atcagtggcg cgccacatgc ccacataccc agcaacgagg agtgcggcat ccgcgatgtg
      241 tggaagcaaa acctggagga ggagttccgc accatacgga aagtggtgca gaagtaccac
      301 tatgtggcca tggacaccga gtttccaggc gtggtagcac gtccagtcgg cgagttccgc
      361 tccacggcgg actatcatta ccagctgctg cgctgtaatg tcgatctgct gcggattatc
      421 caattgggcc ttaccttcat ggacgacgat ggaaagacac cacccggcta ctccacctgg
      481 cagttcaact tcaagtttaa cctgagcgag gacatgtatg cgcaggactc gatcgatctg
      541 ctgcagaact ccggaattca gttcaagaag cacgaggagg atggcatcga tcccatcgac
      601 ttcgccgaac tgctgatgag ctctggcatc gtgctggtgg aaaacatcaa gtggctgtgc
      661 ttccactccg gctacgattt tggctacctt ctaaagctgc tcaccgatca gaacctgccc
      721 ccggacgaat ctgaattttt cgatctgctg cacatctact ttcccaacat cttcgacatc
      781 aagtatctga tgaagtcgtg caagaacctg aaaggcggcc tgcaggaggt tgccgatcag
      841 ctggaactgc ggcgcgtggg cccccagcat caggccggct cggatgctct gctgacgggc
      901 atggccttct tcaaaatgcg tgagatgttc ttcgaggaca acatcgacca tgcgaagtac
      961 tccggacacc tgtacggcct gggtacctcc ttcatcgtca atggcaccaa cttccacgac
     1021 agcaacggtg agacgaacag cgcttcaatc cagcacagtg gcggccgctc gagtctagag
     1081 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     1141 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     1201 tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat aagatacatt
     1261 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1321 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1381 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1441 taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc
     1501 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     1561 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     1621 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     1681 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     1741 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     1801 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     1861 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     1921 tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2401 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     3061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     3361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3721 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3781 gccctttcgt cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa
     3841 aaaatcatga atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt
     3901 ccactgtcac atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca
     3961 cgttttatgg ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg
     4021 tacacattcg agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga
     4081 ggagaaggag aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg
     4141 gccatattcg gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc
     4201 tatatgtttt tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg
     4261 tgtcactcgc tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg
     4321 gcgagtattt tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt
     4381 tctacaaaaa catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag
     4441 attgtatgta tataccttta atgtattctc attttcttat gtatttataa tggcaatgat
     4501 gatactgatg atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg
     4561 ccgaacgcag tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc
     4621 tttgatttca gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc
     4681 aataccaaat gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg
     4741 gtggggcggg gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact
     4801 cctttgacca tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt
     4861 tctttttatg ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat
     4921 gttgccgttt ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat
     4981 tagtcaacga aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg
     5041 tgcagccaac gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg
     5101 tttgcaagcc gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga
     5161 gcagacgaag ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca
     5221 attgcgatat ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa
     5281 taaaacaaat tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa
     5341 gaaatgctta ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa
     5401 agagggagag cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct
     5461 gttgcgcaaa cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata
     5521 tttgcactat tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta
     5581 taccacctga gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac
     5641 ggcatcatta tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc
     5701 cgttattgtt aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact
     5761 agaagatgtg tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt
     5821 aaacgtaagc taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact
     5881 aataaaccca ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg
     5941 gaaaaaatgt gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg
     6001 aatatgagta acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca
     6061 ctcttcatgg cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc
     6121 tccgtaaatg aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa
     6181 cgcgacttga gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat
     6241 aaaacgggct gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga
     6301 tactcctccc gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccg
//
